RLG00000034792

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000466
Physical Location & Seq
Forward (+)
54628594 .. 54632672
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034792

Sequence Viewer

Length: 741 bp
ATGGTGTCGCCGCAAGATCTATTTAGTAAAAGGGTAGTTTCCTACGATTCAACTCCGAAGGCGATGGTGTCGCCGGCAGATCAATTTGGTAAAGGGGGGCTAGCAATAATAATCATAATCCGGCGCCGGGCCGATTTTCTCGCGGAGCTCCAGCTTCAGCAGCTTGTACCCGTGCTTGACGGACAACTGGGGGGAGCTGCCGTTGAAGGAGGACGACTCCGAGGACATGTTGCTCTACGGCGTTCTAAGAGACGTCATCAGCGTGGGGTGGCTCCCGTCTCTCGGGTCCGGGTGCGGCTCGGCCGACGGGTTGCGGTGAAGTCGGAGCTGTGCTCTTCTCGTCGTCTATGGAAGTACGATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCTCCGAGCTGTATGATAGACTAGAACGGAGAGGAATTAAAACCTTTATGGATGACCCCGATCTTCATGTAGGAGATTCCATTAGCCCGTCTCTTGTTTCAGCAATTGAGGAGTCTAAGATTTGCCATTGTCATTCTTTCACCCAACTATGCTTCTTACTCGTCACGATTATTATTCAATGCATGAAAGAGAAGGGGCTACGAGTCATGCCCGTCTTCTACAAAGTGGTGCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAGGGAAACCCCGACCCCGAGTACAAGTAAGGGAACATGAAGAAGAAGTTTATGGCAAGAAAGAATGA

Protein Analysis

247

Amino Acids

27.95

Weight (kDa)

10.12

Isoelectric Point (pI)

64.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 118 - 223 1e-25 TIR domain
TIR_2 PF13676 121 - 209 4.8e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 256
AccB1I GGYRCC 3 cut(s) 123, 631, 648
AccII CGCG 2 cut(s) 143, 391
AciI CCGC 5 cut(s) 11, 143, 295, 314, 646
AcoI YGGCCR 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 2 cut(s) 124, 253
AfaI GTAC 3 cut(s) 168, 356, 696
AfiI CCNNNNNNNGG 2 cut(s) 127, 282
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 3 cut(s) 51, 206, 581
AluBI AGCT 7 cut(s) 148, 154, 163, 197, 328, 412, 670
AluI AGCT 7 cut(s) 148, 154, 163, 197, 328, 412, 670
Alw21I GWGCWC 3 cut(s) 150, 335, 672
Alw26I GTCTC 3 cut(s) 244, 283, 498
Ama87I CYCGRG 2 cut(s) 282, 690
AoxI GGCC 3 cut(s) 129, 301, 380
ApeKI GCWGC 2 cut(s) 160, 197
AspLEI GCGC 2 cut(s) 126, 393
AspS9I GGNCC 3 cut(s) 129, 286, 380
AsuC2I CCSGG 2 cut(s) 128, 290
AsuHPI GGTGA 2 cut(s) 328, 535
AsuNHI GCTAGC 1 cut(s) 100
AvaI CYCGRG 2 cut(s) 282, 690
AvaII GGWCC 1 cut(s) 286
BanI GGYRCC 3 cut(s) 123, 631, 648
BanII GRGCYC 2 cut(s) 150, 672
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 3 cut(s) 150, 335, 672
BbvI GCAGC 2 cut(s) 172, 184
BccI CCATC 1 cut(s) 58
BceAI ACGGC 2 cut(s) 185, 254
BcnI CCSGG 2 cut(s) 128, 290
BcoDI GTCTC 3 cut(s) 244, 283, 498
BfaI CTAG 2 cut(s) 101, 425
BfoI RGCGCY 1 cut(s) 127
BglII AGATCT 1 cut(s) 16
BisI GCNGC 5 cut(s) 11, 161, 198, 296, 647
BlsI GCNGC 5 cut(s) 12, 162, 199, 297, 648
Bme1390I CCNGG 2 cut(s) 128, 290
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 2 cut(s) 282, 690
BmgT120I GGNCC 3 cut(s) 129, 286, 380
BmiI GGNNCC 5 cut(s) 125, 273, 287, 633, 650
BmrFI CCNGG 2 cut(s) 128, 290
BmrI ACTGGG 1 cut(s) 197
BmtI GCTAGC 1 cut(s) 104
BmuI ACTGGG 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 610
BplI GAGNNNNNCTC 4 cut(s) 201, 233, 317, 349
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 128, 290
BsaHI GRCGYC 2 cut(s) 124, 253
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 282
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 127, 282
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 527
BseX3I CGGCCG 1 cut(s) 301
BseXI GCAGC 2 cut(s) 172, 184
Bsh1236I CGCG 2 cut(s) 143, 391
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 131, 303, 382
BshNI GGYRCC 3 cut(s) 123, 631, 648
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 3 cut(s) 150, 335, 672
BsiHKCI CYCGRG 2 cut(s) 282, 690
BsiSI CCGG 4 cut(s) 74, 121, 127, 289
BslI CCNNNNNNNGG 2 cut(s) 127, 282
BsmAI GTCTC 3 cut(s) 244, 283, 498
BsmBI CGTCTC 3 cut(s) 244, 283, 498
BsnI GGCC 3 cut(s) 131, 303, 382
BsoBI CYCGRG 2 cut(s) 282, 690
Bsp1286I GDGCHC 3 cut(s) 150, 335, 672
Bsp143I GATC 3 cut(s) 16, 79, 463
BspACI CCGC 5 cut(s) 11, 143, 295, 314, 646
BspANI GGCC 3 cut(s) 131, 303, 382
BspFNI CGCG 2 cut(s) 143, 391
BspLI GGNNCC 5 cut(s) 125, 273, 287, 633, 650
BspOI GCTAGC 1 cut(s) 104
BspQI GCTCTTC 1 cut(s) 340
BspT107I GGYRCC 3 cut(s) 123, 631, 648
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 16, 79, 463
BssNI GRCGYC 2 cut(s) 124, 253
Bst6I CTCTTC 1 cut(s) 340
BstACI GRCGYC 2 cut(s) 124, 253
BstC8I GCNNGC 2 cut(s) 75, 102
BstDEI CTNAG 3 cut(s) 246, 368, 519
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 2 cut(s) 143, 391
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 2 cut(s) 126, 393
BstKTI GATC 3 cut(s) 19, 82, 466
BstMAI GTCTC 3 cut(s) 244, 283, 498
BstMBI GATC 3 cut(s) 16, 79, 463
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 2 cut(s) 160, 388
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 126, 288
BstUI CGCG 2 cut(s) 143, 391
BstV1I GCAGC 2 cut(s) 172, 184
BstV2I GAAGAC 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BstZI CGGCCG 1 cut(s) 301
BsuRI GGCC 3 cut(s) 131, 303, 382
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 75, 102
CfoI GCGC 2 cut(s) 126, 393
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 3 cut(s) 129, 286, 380
Csp6I GTAC 3 cut(s) 167, 355, 695
CviAII CATG 5 cut(s) 227, 470, 586, 610, 710
CviQI GTAC 3 cut(s) 167, 355, 695
DdeI CTNAG 3 cut(s) 246, 368, 519
DinI GGCGCC 1 cut(s) 125
DpnI GATC 3 cut(s) 18, 81, 465
DpnII GATC 3 cut(s) 16, 79, 463
EaeI YGGCCR 1 cut(s) 301
EagI CGGCCG 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Ecl136II GAGCTC 2 cut(s) 148, 670
EclXI CGGCCG 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 150, 672
Eco47I GGWCC 1 cut(s) 286
Eco52I CGGCCG 1 cut(s) 301
Eco53kI GAGCTC 2 cut(s) 148, 670
Eco57I CTGAAG 1 cut(s) 140
Eco88I CYCGRG 2 cut(s) 282, 690
EcoICRI GAGCTC 2 cut(s) 148, 670
EcoT22I ATGCAT 1 cut(s) 587
EcoT38I GRGCYC 2 cut(s) 150, 672
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
Esp3I CGTCTC 3 cut(s) 244, 283, 498
FaeI CATG 5 cut(s) 230, 473, 589, 613, 713
FatI CATG 5 cut(s) 226, 469, 585, 609, 709
Fnu4HI GCNGC 5 cut(s) 11, 161, 198, 296, 647
FokI GGATG 1 cut(s) 467
FriOI GRGCYC 2 cut(s) 150, 672
Fsp4HI GCNGC 5 cut(s) 11, 161, 198, 296, 647
FspBI CTAG 2 cut(s) 101, 425
GlaI GCGC 2 cut(s) 125, 392
GluI GCNGC 5 cut(s) 11, 161, 198, 296, 647
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 3 cut(s) 131, 303, 382
HapII CCGG 4 cut(s) 74, 121, 127, 289
HhaI GCGC 2 cut(s) 126, 393
Hin1I GRCGYC 2 cut(s) 124, 253
Hin1II CATG 5 cut(s) 230, 473, 589, 613, 713
Hin6I GCGC 2 cut(s) 124, 391
HinP1I GCGC 2 cut(s) 124, 391
HinfI GANTC 5 cut(s) 47, 216, 479, 515, 606
HpaII CCGG 4 cut(s) 74, 121, 127, 289
HphI GGTGA 2 cut(s) 328, 535
Hpy188I TCNGA 6 cut(s) 57, 221, 325, 377, 409, 640
Hpy188III TCNNGA 1 cut(s) 568
Hpy99I CGWCG 2 cut(s) 309, 345
HpyAV CCTTC 4 cut(s) 52, 200, 589, 645
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 1 cut(s) 585
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 388
HpyF3I CTNAG 3 cut(s) 246, 368, 519
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 2 cut(s) 124, 253
Hsp92II CATG 5 cut(s) 230, 473, 589, 613, 713
HspAI GCGC 2 cut(s) 124, 391
KasI GGCGCC 1 cut(s) 123
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Kzo9I GATC 3 cut(s) 16, 79, 463
LguI GCTCTTC 1 cut(s) 340
LmnI GCTCC 5 cut(s) 145, 153, 194, 277, 325
LpnPI CCDG 7 cut(s) 87, 134, 140, 164, 173, 302, 396
Lsp1109I GCAGC 2 cut(s) 172, 184
MaeI CTAG 2 cut(s) 101, 425
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 565
MalI GATC 3 cut(s) 18, 81, 465
MboI GATC 3 cut(s) 16, 79, 463
MboII GAAGA 5 cut(s) 327, 458, 610, 725, 728
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 3 cut(s) 150, 335, 672
MluCI AATT 3 cut(s) 83, 438, 507
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 3 cut(s) 210, 524, 615
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 6 cut(s) 203, 215, 371, 415, 428, 505
Mph1103I ATGCAT 1 cut(s) 587
MroNI GCCGGC 1 cut(s) 73
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 261
MspI CCGG 4 cut(s) 74, 121, 127, 289
MspR9I CCNGG 2 cut(s) 128, 290
MunI CAATTG 1 cut(s) 507
MvnI CGCG 2 cut(s) 143, 391
MwoI GCNNNNNNNGC 2 cut(s) 160, 388
NaeI GCCGGC 1 cut(s) 75
NarI GGCGCC 1 cut(s) 124
NciI CCSGG 2 cut(s) 128, 290
NdeII GATC 3 cut(s) 16, 79, 463
NgoMIV GCCGGC 1 cut(s) 73
NheI GCTAGC 1 cut(s) 100
NlaIII CATG 5 cut(s) 230, 473, 589, 613, 713
NlaIV GGNNCC 5 cut(s) 125, 273, 287, 633, 650
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 565
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 230
PciI ACATGT 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 47, 479
PkrI GCNGC 5 cut(s) 12, 162, 199, 297, 648
PleI GAGTC 3 cut(s) 210, 523, 614
PluTI GGCGCC 1 cut(s) 127
PpsI GAGTC 3 cut(s) 210, 523, 614
PscI ACATGT 1 cut(s) 226
Psp124BI GAGCTC 2 cut(s) 150, 672
PspN4I GGNNCC 5 cut(s) 125, 273, 287, 633, 650
PspPI GGNCC 3 cut(s) 129, 286, 380
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 3 cut(s) 168, 356, 696
RsaNI GTAC 3 cut(s) 167, 355, 695
RseI CAYNNNNRTG 1 cut(s) 261
SacI GAGCTC 2 cut(s) 150, 672
SapI GCTCTTC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 5 cut(s) 11, 161, 198, 296, 647
Sau3AI GATC 3 cut(s) 16, 79, 463
Sau96I GGNCC 3 cut(s) 129, 286, 380
SchI GAGTC 3 cut(s) 210, 524, 615
ScrFI CCNGG 2 cut(s) 128, 290
SduI GDGCHC 3 cut(s) 150, 335, 672
SfoI GGCGCC 1 cut(s) 125
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 261
Sse9I AATT 3 cut(s) 83, 438, 507
SsiI CCGC 5 cut(s) 11, 143, 295, 314, 646
SspDI GGCGCC 1 cut(s) 123
SspMI CTAG 2 cut(s) 101, 425
SstI GAGCTC 2 cut(s) 150, 672
StyD4I CCNGG 2 cut(s) 126, 288
TaiI ACGT 1 cut(s) 256
TasI AATT 3 cut(s) 83, 438, 507
TatI WGTACW 1 cut(s) 694
TauI GCSGC 3 cut(s) 13, 298, 649
TfiI GAWTC 2 cut(s) 47, 479
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 2 cut(s) 160, 197
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 3 cut(s) 458, 602, 726
TspGWI ACGGA 2 cut(s) 195, 445
VpaK11BI GGWCC 1 cut(s) 286
XceI RCATGY 1 cut(s) 230
XspI CTAG 2 cut(s) 101, 425
ZraI GACGTC 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.