Rorug07G0125600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000466
Physical Location & Seq
Forward (+)
9896262 .. 9901482
5221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0125600.1

Sequence Viewer

Length: 3336 bp
ATGCCTGTAAATCCATGGACCTTCCTCTTCTTCTTAACCCTCTCTTCTATTCTTCTGGTTCACTTTCCTGCTCTGGCCTTGAACCAACAAGGCCAGGCTCTTGTTTCATGGAAGCAGAGTCTCAATGGCTCCCCTGAGGGGCTGAGCAATTGGGACCCAAGTGATGAAACTCCATGCGGGTGGTCCGGGGTCACCTGCAACTTCAACAACCAAGTTGTGGAGCTGAATTTGAAGTATATTGATCTTCTAGGTAAGGTGCCCTGGAATTTCACTTCGTTGCTGACCCTAAACAAGCTCATCTTGTCCGGCACAAACCTCACCGGCTCAATCCCACGAGAAATCTCTACTCTTAAACAACTGACCTCACTGGACCTGAGCGACAATGCACTGAGCGGCGAAATCCCAGTTGAGATCTGCGAGCTGCCTAAGCTCGAAGAACTCTACCTCAGCACGAACCGGCTGGAGGGCTCAATCCCAGTCCAAATCGGAAACCTCACAAACCTGAAGTGGCTGGTTCTCTACGACAATCAGCTCAGCGGGAAGATTCCTAGCACCATTGGAAATCTGCCTCAACTTCAAGTAATCAGAGCTGGAGGGAACAAGAACCTTGAAGGTCCTGTGCCTCATGAGATTGGGAACTGCACCGATTTGGTCATGTTGGGATTAGCAGAAACCAGCATTTCCGGCTTCCTCCCTCCAAGCCTTGGCCTCCTCAAAAAACTCGAGACATTGGCAATCTACACCACCCTCGTCTCCGGTCCGATCCCCCAAGAGCTCGGAGACTGCACCAAGCTCCGGGACATCTACTTGTACGAAAACTCCATTAGCAGCTCTGTTCCAAGCAAATTAGGCAACTTGAAAAATCTTCAAAACCTTCTGCTATGGCAAAACAGCCTTGTAGGGGTTATTCCACCGGAGCTCGGCAATTGTCACCAGCTATTGGTCATTGACATTTCTATGAATTCCTTGACCGGAAGCATTCCTCAGTCTTTTGGGAACTTGACTTCGCTTCAGGAGCTCCAATTGAGTGTCAATCAAATCTCAGGCGAGATCCCGGCGCAGCTAGGAAACTGCCGGACCCTCACTCATATCGAGCTGGATAACAACCAGATCACCGGAACGATTCCTTTCGAATTCGGCAGCTTATCCAATCTCACGGTCCTCTTCTTGTGGCAAAACAAGCTTGAAGGAGCCATTCCTTCGTCGATTTCCAACTGTGGCAATCTTGAAGCGGTTGATTTCTCACAAAACTTTTTGACCGGTCCGATTCCGGGTGGGATTTTCCAGCTCCAGAATCTCACCAAGCTTCTACTACTGTCGAACAATCTCTCCAGCGAAATCCCGCCGGAGATCGGAAACTGTTCCTCGTTGATTCGGTTTCGGGCGAACAACAACAAGCTCACCGGAGCAATACCGCAGCAGATTGGGAGTTTGAAGAGCTTGAATTTCCTCGATCTCGGATCGAATCGGCTCAACCGAAACATACCGGAGGGGATCGCCGGCTGCCGGAATCTGACGTTTCTCGACCTGCATTCCAATTCGATCACCGGAAACTTGCCTGCAGGTTTCAATCTGCTTGTGTCTCTCCAGTTCGTCGATTTCTCGGATAATATGATCGAAGGGACGTTGAGTCCTGGACTCGGTTCGCTGACGTCACTCACCAAGCTCGTTTTGGGGAACAACCGGTTCGCCGGTTCCATTCCCAGTCAACTCGGTTCGTGTGGGAAGTTACAGTTACTGGACCTTGGCGGGAACGAGCTAACAGGTACAATCCCGGTGAGTTTGGGAAAGATTCCGGCACTGGAAATCTCTCTGAACCTGAGCTGGAACCAACTCTCCGGCGAGTTACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGCATTCTGGACCTCTCTTACAACCAGCTCTCCGGCGACCTTCAATTTCTCGCTGACATGCAAAATCTCGTCGTCCTTAACGTCTCGCACAACAACTTCACCGGGCGCGTGCCGGACACGCCCTTCTTCGCGAAGCTCCCTCTCAGCGTCATGTCCGGCAACCCCTCCCTCTGCCTCACCGGCAGCCAGTGCGCCGACGCAGGAAACCCCGGCTGGTCCCACCGCCGCAACGCGGCCGCGCGCGTGGCGATGGTCATCCTCCTCTGCACCGCCTGCACACTCCTCTTAGCGGCGCTCTACATCATCCTCGCAGCCCGAAAGCGGGCCCGGCCAGGGTTCTTCAGCGGGGCCCACGAGCCCGATCCGGAGGACGACACCGAAGTAGACGTGGGCCCACCGTGGGAGGTGACGCTCTACCAGAAACTCGACCTGTCGATCGCCAACGTGGCGAAAAGCCTGACGCCTGACAACGTCATAGGCCGGGGGCGATCCGGCGTCGTTTACCACGTGTCAATCGCCTCGGGACTCTCCCTTGCCGTGAAAAGATTCCGTACGGCCGAGAAACACTCCGCCGCCGCTTTTTCCTCCGAGATTGCCACGCTGGCAAGGATACGTCACCGTAACATTGTCAGGCTACTGGGCTGGGGGGCCAACCGGAGAACGAAGTTACTGTTTTACGATTACTTGGCACAGGGTAATTTGGGCTCGTTGCTCCACGAGGGGTGCGCAGGATTAGTAGAGTGGGACACGCGGTTCAAGATAGCACTAGGCGTGGCGGAGGGTTTGGCTTATTTGCACCACGATTGCCAGCCGGCTATTTTGCACCGGGATGTGAAGGCCCAGAACATTTTGCTGGGGGATCAATACGAGGCCGTTTTGGCAGACTTTGGGCTCGCTAGGTTGGTTGAAGACGATCGAAACGGTCCGTTTTCGGCCAACCCCCAGTTTGCTGGTTCATACGGCTACATTGCGCCTGAATATGCCTGTATGCTGAAAATTACCGCGAAAAGTGATGTGTATAGCTATGGGGTAGTCCTTTTGGAGATCATAACTGGGAAAAGGCCAGCAGACCCCTCATTCACCGAAGGCCAGCATGTGATTCAGTGGGTGCGAGACCACTTGAAGAGCAAGAAGGATCCAATAGAGATTCTTGATCAGAAGCTCCAAGGCTACCCGGATACGCAGATACAAGAAATGCTCCAAGCGCTTGGAATTTCGCTCCTATGTACCAGCAACAGGGCGGAAGATCGTCCCACGATGAAGGATGTGGCTGCATTGTTGAGAGAAATTCGACATGATCAGCCTGCGACAGGAAGCGAGGCATACAAGCCTGCTAGCACCGCGTTGAAGAACTCATCTTCTTCCGTGACTCCAGCTCAACTGCTGATGATGCAAGGATCTTCTCATTGCTCACTTGCCTACTCTTCTTCGGCTGGTTATCTATCAGGAACCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1111

Amino Acids

120.34

Weight (kDa)

5.65

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 8.2e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 196 6.1e-11 Leucine-rich repeat region
LRR_8 PF13855 96 - 154 2.1e-07 Leucine rich repeat
LRR_8 PF13855 142 - 201 8e-07 Leucine rich repeat
LRR_14 PF23598 152 - 248 1.9e-08 Leucine-rich repeat region
LRR_14 PF23598 257 - 367 2.5e-09 Leucine-rich repeat region
LRR_8 PF13855 288 - 347 1e-07 Leucine rich repeat
LRR_8 PF13855 463 - 515 6.8e-06 Leucine rich repeat
LRR_14 PF23598 469 - 661 1.2e-10 Leucine-rich repeat region
LRR_8 PF13855 552 - 612 2.6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 782 - 1051 1e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 783 - 1049 6.3e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
AatII GACGTC 1 cut(s) 1655
Acc16I TGCGCA 1 cut(s) 2605
Acc36I ACCTGC 3 cut(s) 203, 1536, 1553
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 3 cut(s) 217, 704, 940
AccBSI CCGCTC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 2262
AccII CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
AccIII TCCGGA 1 cut(s) 2242
AcoI YGGCCR 4 cut(s) 2112, 2207, 2433, 2811
AcsI RAATTY 7 cut(s) 226, 265, 961, 1133, 1444, 3090, 3165
AcuI CTGAAG 3 cut(s) 524, 995, 2203
AcvI CACGTG 1 cut(s) 2386
AcyI GRCGYC 3 cut(s) 1652, 2339, 2373
AfaI GTAC 4 cut(s) 812, 1768, 2431, 3106
AfeI AGCGCT 1 cut(s) 3084
AflIII ACRYGT 1 cut(s) 2385
AgeI ACCGGT 2 cut(s) 1259, 1683
AhdI GACNNNNNGTC 1 cut(s) 1629
AjiI CACGTC 1 cut(s) 2266
AjnI CCWGG 4 cut(s) 93, 260, 1633, 2209
AloI GAACNNNNNNTCC 6 cut(s) 1313, 1345, 1820, 1852, 2615, 2647
Alw21I GWGCWC 3 cut(s) 777, 921, 1020
Alw26I GTCTC 7 cut(s) 125, 719, 757, 774, 1587, 1966, 2985
AlwNI CAGNNNCTG 1 cut(s) 1738
Ama87I CYCGRG 2 cut(s) 722, 2398
Aor13HI TCCGGA 1 cut(s) 2242
Aor51HI AGCGCT 1 cut(s) 3084
ApaI GGGCCC 3 cut(s) 2206, 2230, 2273
ApeKI GCWGC 9 cut(s) 421, 828, 1060, 1140, 1417, 1503, 2061, 2189, 3149
ApoI RAATTY 7 cut(s) 226, 265, 961, 1133, 1444, 3090, 3165
AsiGI ACCGGT 2 cut(s) 1259, 1683
Asp700I GAANNNNTTC 3 cut(s) 1360, 1856, 2423
AspLEI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
AsuII TTCGAA 1 cut(s) 1131
AsuNHI GCTAGC 1 cut(s) 3212
AvaI CYCGRG 2 cut(s) 722, 2398
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BamHI GGATCC 1 cut(s) 3013
BanI GGYRCC 1 cut(s) 256
BauI CACGAG 3 cut(s) 333, 2231, 2594
BbrPI CACGTG 1 cut(s) 2386
BbsI GAAGAC 1 cut(s) 2793
Bbv12I GWGCWC 3 cut(s) 777, 921, 1020
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 9 cut(s) 408, 840, 1072, 1152, 1429, 1490, 2073, 2201, 3136
BccI CCATC 1 cut(s) 2122
BceAI ACGGC 4 cut(s) 2399, 2448, 2735, 2854
BciT130I CCWGG 4 cut(s) 95, 262, 1635, 2211
BciVI GTATCC 2 cut(s) 2481, 3049
BclI TGATCA 2 cut(s) 3031, 3175
BcoDI GTCTC 7 cut(s) 125, 719, 757, 774, 1587, 1966, 2985
BfaI CTAG 6 cut(s) 248, 549, 1064, 2645, 2775, 3213
BfmI CTRYAG 1 cut(s) 1560
BfoI RGCGCY 2 cut(s) 2174, 3086
BfuAI ACCTGC 3 cut(s) 203, 1536, 1553
BfuI GTATCC 2 cut(s) 2481, 3049
BglI GCCNNNNNGGC 4 cut(s) 2058, 2324, 2480, 2756
BglII AGATCT 1 cut(s) 411
BlpI GCTNAGC 2 cut(s) 143, 533
BmeRI GACNNNNNGTC 1 cut(s) 1629
BmeT110I CYCGRG 2 cut(s) 722, 2398
BmgBI CACGTC 1 cut(s) 2266
BmrI ACTGGG 6 cut(s) 398, 470, 1698, 2525, 2815, 2940
BmsI GCATC 1 cut(s) 3258
BmtI GCTAGC 1 cut(s) 3216
BmuI ACTGGG 6 cut(s) 398, 470, 1698, 2525, 2815, 2940
BpiI GAAGAC 1 cut(s) 2793
BplI GAGNNNNNCTC 2 cut(s) 2429, 2461
BpmI CTGGAG 6 cut(s) 482, 612, 1274, 1315, 1571, 3234
Bpu10I CCTNAGC 4 cut(s) 374, 426, 446, 1820
Bpu1102I GCTNAGC 2 cut(s) 143, 533
Bpu14I TTCGAA 1 cut(s) 1131
BsaAI YACGTR 1 cut(s) 2386
BsaBI GATNNNNATC 1 cut(s) 2132
BsaHI GRCGYC 3 cut(s) 1652, 2339, 2373
BsaI GGTCTC 1 cut(s) 2985
BsaXI ACNNNNNCTCC 8 cut(s) 803, 833, 1313, 1343, 1820, 1850, 1892, 1922
Bse118I RCCGGY 8 cut(s) 320, 456, 1259, 1499, 1683, 1691, 2057, 2689
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 2 cut(s) 2844, 3283
Bse8I GATNNNNATC 1 cut(s) 2132
BseAI TCCGGA 1 cut(s) 2242
BseBI CCWGG 4 cut(s) 95, 262, 1635, 2211
BseGI GGATG 4 cut(s) 2133, 2181, 2713, 3148
BseJI GATNNNNATC 1 cut(s) 2132
BseMI GCAATG 2 cut(s) 2844, 3283
BsePI GCGCGC 1 cut(s) 2117
BseRI GAGGAG 3 cut(s) 701, 2129, 2150
BseSI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BseX3I CGGCCG 2 cut(s) 2112, 2433
BseXI GCAGC 9 cut(s) 408, 840, 1072, 1152, 1429, 1490, 2073, 2201, 3136
BseYI CCCAGC 2 cut(s) 2520, 2731
BsgI GTGCAG 4 cut(s) 625, 769, 2128, 2137
Bsh1236I CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
Bsh1285I CGRYCG 4 cut(s) 2115, 2316, 2436, 2794
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 2 cut(s) 1259, 1683
BsiEI CGRYCG 4 cut(s) 2115, 2316, 2436, 2794
BsiHKAI GWGCWC 3 cut(s) 777, 921, 1020
BsiHKCI CYCGRG 2 cut(s) 722, 2398
BsiWI CGTACG 1 cut(s) 2429
BslFI GGGAC 7 cut(s) 167, 812, 1636, 2080, 2415, 2636, 3114
BsmAI GTCTC 7 cut(s) 125, 719, 757, 774, 1587, 1966, 2985
BsmBI CGTCTC 2 cut(s) 757, 1966
BsmFI GGGAC 7 cut(s) 167, 812, 1636, 2080, 2415, 2636, 3114
BsmI GAATGC 3 cut(s) 978, 1531, 1881
Bso31I GGTCTC 1 cut(s) 2985
BsoBI CYCGRG 2 cut(s) 722, 2398
Bsp119I TTCGAA 1 cut(s) 1131
Bsp120I GGGCCC 3 cut(s) 2202, 2226, 2269
Bsp13I TCCGGA 1 cut(s) 2242
Bsp1720I GCTNAGC 2 cut(s) 143, 533
Bsp19I CCATGG 1 cut(s) 14
Bsp68I TCGCGA 1 cut(s) 2009
BspEI TCCGGA 1 cut(s) 2242
BspFNI CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
BspHI TCATGA 1 cut(s) 625
BspMAI CTGCAG 1 cut(s) 1564
BspMI ACCTGC 3 cut(s) 203, 1536, 1553
BspOI GCTAGC 1 cut(s) 3216
BspQI GCTCTTC 2 cut(s) 1430, 2996
BspT104I TTCGAA 1 cut(s) 1131
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 2985
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 2844, 3283
BsrFI RCCGGY 8 cut(s) 320, 456, 1259, 1499, 1683, 1691, 2057, 2689
BssAI RCCGGY 8 cut(s) 320, 456, 1259, 1499, 1683, 1691, 2057, 2689
BssHII GCGCGC 1 cut(s) 2117
BssNI GRCGYC 3 cut(s) 1652, 2339, 2373
BssSI CACGAG 3 cut(s) 333, 2231, 2594
BssT1I CCWWGG 4 cut(s) 14, 703, 1744, 3043
Bst2BI CACGAG 3 cut(s) 333, 2231, 2594
Bst2UI CCWGG 4 cut(s) 95, 262, 1635, 2211
Bst4CI ACNGT 9 cut(s) 1159, 1217, 1317, 1361, 1734, 2277, 2498, 2550, 2801
Bst6I CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
BstACI GRCGYC 3 cut(s) 1652, 2339, 2373
BstAPI GCANNNNNTGC 2 cut(s) 2067, 2151
BstBAI YACGTR 1 cut(s) 2386
BstBI TTCGAA 1 cut(s) 1131
BstDSI CCRYGG 2 cut(s) 14, 2276
BstEII GGTNACC 1 cut(s) 190
BstENI CCTNNNNNAGG 1 cut(s) 3186
BstF5I GGATG 4 cut(s) 2133, 2181, 2713, 3148
BstFNI CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
BstH2I RGCGCY 2 cut(s) 2174, 3086
BstHHI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
BstMAI GTCTC 7 cut(s) 125, 719, 757, 774, 1587, 1966, 2985
BstMCI CGRYCG 4 cut(s) 2115, 2316, 2436, 2794
BstNI CCWGG 4 cut(s) 95, 262, 1635, 2211
BstNSI RCATGY 2 cut(s) 1939, 2975
BstPI GGTNACC 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 1560
BstSLI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BstUI CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
BstV1I GCAGC 9 cut(s) 408, 840, 1072, 1152, 1429, 1490, 2073, 2201, 3136
BstV2I GAAGAC 1 cut(s) 2793
BstX2I RGATCY 4 cut(s) 411, 1050, 3013, 3275
BstXI CCANNNNNNTGG 3 cut(s) 180, 2828, 3086
BstYI RGATCY 4 cut(s) 411, 1050, 3013, 3275
BstZI CGGCCG 2 cut(s) 2112, 2433
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 2 cut(s) 2481, 3049
BtgI CCRYGG 2 cut(s) 14, 2276
BtgZI GCGATG 1 cut(s) 2141
BtrI CACGTC 1 cut(s) 2266
BtsCI GGATG 4 cut(s) 2133, 2181, 2713, 3148
BtsIMutI CAGTG 5 cut(s) 365, 386, 1799, 2072, 2987
BtuMI TCGCGA 1 cut(s) 2009
BveI ACCTGC 3 cut(s) 203, 1536, 1553
CaiI CAGNNNCTG 1 cut(s) 1738
CciI TCATGA 1 cut(s) 625
CciNI GCGGCCGC 1 cut(s) 2112
CfoI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
Cfr10I RCCGGY 8 cut(s) 320, 456, 1259, 1499, 1683, 1691, 2057, 2689
CpoI CGGWCCG 3 cut(s) 758, 1262, 2801
CseI GACGC 5 cut(s) 2014, 2084, 2296, 2347, 2362
Csp6I GTAC 4 cut(s) 811, 1767, 2430, 3105
CspAI ACCGGT 2 cut(s) 1259, 1683
CspI CGGWCCG 3 cut(s) 758, 1262, 2801
CviAII CATG 9 cut(s) 15, 108, 174, 626, 655, 1936, 2029, 2972, 3173
CviQI GTAC 4 cut(s) 811, 1767, 2430, 3105
DriI GACNNNNNGTC 1 cut(s) 1629
EaeI YGGCCR 4 cut(s) 2112, 2207, 2433, 2811
EagI CGGCCG 2 cut(s) 2112, 2433
Eam1104I CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
Eam1105I GACNNNNNGTC 1 cut(s) 1629
EarI CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
EciI GGCGGA 3 cut(s) 2437, 2669, 3134
Ecl136II GAGCTC 3 cut(s) 775, 919, 1018
EclXI CGGCCG 2 cut(s) 2112, 2433
Eco130I CCWWGG 4 cut(s) 14, 703, 1744, 3043
Eco31I GGTCTC 1 cut(s) 2985
Eco47III AGCGCT 1 cut(s) 3084
Eco52I CGGCCG 2 cut(s) 2112, 2433
Eco53kI GAGCTC 3 cut(s) 775, 919, 1018
Eco57I CTGAAG 3 cut(s) 524, 995, 2203
Eco72I CACGTG 1 cut(s) 2386
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 2 cut(s) 722, 2398
Eco91I GGTNACC 1 cut(s) 190
EcoICRI GAGCTC 3 cut(s) 775, 919, 1018
EcoNI CCTNNNNNAGG 1 cut(s) 3186
EcoO109I RGGNCCY 3 cut(s) 154, 614, 2226
EcoO65I GGTNACC 1 cut(s) 190
EcoRI GAATTC 2 cut(s) 961, 1133
EcoRII CCWGG 4 cut(s) 93, 260, 1633, 2209
EcoT14I CCWWGG 4 cut(s) 14, 703, 1744, 3043
ErhI CCWWGG 4 cut(s) 14, 703, 1744, 3043
Esp3I CGTCTC 2 cut(s) 757, 1966
FaeI CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 7 cut(s) 167, 812, 1636, 2080, 2415, 2636, 3114
FatI CATG 9 cut(s) 14, 107, 173, 625, 654, 1935, 2028, 2971, 3172
FauI CCCGC 6 cut(s) 170, 530, 1350, 1742, 2193, 2216
FbaI TGATCA 2 cut(s) 3031, 3175
FblI GTMKAC 1 cut(s) 2262
FokI GGATG 4 cut(s) 2120, 2168, 2720, 3155
FspBI CTAG 6 cut(s) 248, 549, 1064, 2645, 2775, 3213
FspI TGCGCA 1 cut(s) 2605
GlaI GCGC 9 cut(s) 1059, 1985, 2071, 2118, 2120, 2172, 2605, 2850, 3084
GsaI CCCAGC 2 cut(s) 2524, 2735
GsuI CTGGAG 6 cut(s) 482, 612, 1274, 1315, 1571, 3234
HaeII RGCGCY 2 cut(s) 2174, 3086
HgaI GACGC 5 cut(s) 2014, 2084, 2296, 2347, 2362
HhaI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
Hin1I GRCGYC 3 cut(s) 1652, 2339, 2373
Hin1II CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
Hin6I GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
HinP1I GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
HincII GTYRAC 1 cut(s) 1709
HindII GTYRAC 1 cut(s) 1709
HindIII AAGCTT 2 cut(s) 1181, 1304
Hpy166II GTNNAC 4 cut(s) 61, 1709, 2263, 2380
Hpy8I GTNNAC 4 cut(s) 61, 1709, 2263, 2380
Hpy99I CGWCG 5 cut(s) 1207, 1598, 1952, 2078, 2378
HpyCH4III ACNGT 9 cut(s) 1159, 1217, 1317, 1361, 1734, 2277, 2498, 2550, 2801
HpyCH4IV ACGT 9 cut(s) 1517, 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
HpySE526I ACGT 9 cut(s) 1517, 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
Hsp92I GRCGYC 3 cut(s) 1652, 2339, 2373
Hsp92II CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
HspAI GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 2242
KroI GCCGGC 2 cut(s) 1499, 2689
KroNI GCCGGC 2 cut(s) 1501, 2691
Ksp22I TGATCA 2 cut(s) 3031, 3175
LguI GCTCTTC 2 cut(s) 1430, 2996
Lsp1109I GCAGC 9 cut(s) 408, 840, 1072, 1152, 1429, 1490, 2073, 2201, 3136
LweI GCATC 1 cut(s) 3258
MaeI CTAG 6 cut(s) 248, 549, 1064, 2645, 2775, 3213
MaeII ACGT 9 cut(s) 1517, 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
MauBI CGCGCGCG 1 cut(s) 2117
MbiI CCGCTC 1 cut(s) 393
MfeI CAATTG 3 cut(s) 148, 925, 1022
MflI RGATCY 4 cut(s) 411, 1050, 3013, 3275
MlyI GAGTC 5 cut(s) 127, 1632, 1639, 2397, 3241
MmeI TCCRAC 1 cut(s) 1236
MroI TCCGGA 1 cut(s) 2242
MroNI GCCGGC 2 cut(s) 1499, 2689
MroXI GAANNNNTTC 3 cut(s) 1360, 1856, 2423
MseI TTAA 3 cut(s) 35, 351, 1956
MslI CAYNNNNRTG 3 cut(s) 178, 956, 2706
MspA1I CMGCKG 2 cut(s) 537, 2223
MunI CAATTG 3 cut(s) 148, 925, 1022
Mva1269I GAATGC 3 cut(s) 978, 1531, 1881
MvaI CCWGG 4 cut(s) 95, 262, 1635, 2211
MvnI CGCG 9 cut(s) 1986, 2009, 2111, 2117, 2119, 2121, 2629, 2882, 3221
NaeI GCCGGC 2 cut(s) 1501, 2691
NcoI CCATGG 1 cut(s) 14
NgoMIV GCCGGC 2 cut(s) 1499, 2689
NheI GCTAGC 1 cut(s) 3212
NlaIII CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
NmeAIII GCCGAG 3 cut(s) 900, 1857, 2461
NmuCI GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
NotI GCGGCCGC 1 cut(s) 2112
NruI TCGCGA 1 cut(s) 2009
NsbI TGCGCA 1 cut(s) 2605
NspI RCATGY 2 cut(s) 1939, 2975
NspV TTCGAA 1 cut(s) 1131
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 203
PauI GCGCGC 1 cut(s) 2117
PciSI GCTCTTC 2 cut(s) 1430, 2996
PcsI WCGNNNNNNNCGW 3 cut(s) 1474, 1956, 2382
PctI GAATGC 3 cut(s) 978, 1531, 1881
PdiI GCCGGC 2 cut(s) 1501, 2691
PdmI GAANNNNTTC 3 cut(s) 1360, 1856, 2423
Pfl23II CGTACG 1 cut(s) 2429
PflFI GACNNNGTC 1 cut(s) 2348
PflMI CCANNNNNTGG 3 cut(s) 217, 704, 940
PfoI TCCNGGA 2 cut(s) 795, 1633
PinAI ACCGGT 2 cut(s) 1259, 1683
Ple19I CGATCG 2 cut(s) 2316, 2794
PleI GAGTC 5 cut(s) 126, 1632, 1638, 2397, 3241
PmaCI CACGTG 1 cut(s) 2386
PmlI CACGTG 1 cut(s) 2386
PpsI GAGTC 5 cut(s) 126, 1632, 1638, 2397, 3241
Ppu21I YACGTR 1 cut(s) 2386
PpuMI RGGWCCY 2 cut(s) 154, 614
Psp124BI GAGCTC 3 cut(s) 777, 921, 1020
Psp5II RGGWCCY 2 cut(s) 154, 614
Psp6I CCWGG 4 cut(s) 93, 260, 1633, 2209
PspCI CACGTG 1 cut(s) 2386
PspEI GGTNACC 1 cut(s) 190
PspFI CCCAGC 2 cut(s) 2520, 2731
PspGI CCWGG 4 cut(s) 93, 260, 1633, 2209
PspLI CGTACG 1 cut(s) 2429
PspOMI GGGCCC 3 cut(s) 2202, 2226, 2269
PspPPI RGGWCCY 2 cut(s) 154, 614
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 1738
PsuI RGATCY 4 cut(s) 411, 1050, 3013, 3275
PsyI GACNNNGTC 1 cut(s) 2348
PteI GCGCGC 1 cut(s) 2117
PvuI CGATCG 2 cut(s) 2316, 2794
RruI TCGCGA 1 cut(s) 2009
RsaI GTAC 4 cut(s) 812, 1768, 2431, 3106
RsaNI GTAC 4 cut(s) 811, 1767, 2430, 3105
RseI CAYNNNNRTG 3 cut(s) 178, 956, 2706
Rsr2I CGGWCCG 3 cut(s) 758, 1262, 2801
RsrII CGGWCCG 3 cut(s) 758, 1262, 2801
SacI GAGCTC 3 cut(s) 777, 921, 1020
SapI GCTCTTC 2 cut(s) 1430, 2996
SaqAI TTAA 3 cut(s) 35, 351, 1956
SbfI CCTGCAGG 1 cut(s) 1564
SchI GAGTC 5 cut(s) 127, 1632, 1639, 2397, 3241
SdaI CCTGCAGG 1 cut(s) 1564
SfaNI GCATC 1 cut(s) 3258
SfcI CTRYAG 1 cut(s) 1560
Sfr274I CTCGAG 1 cut(s) 722
SfuI TTCGAA 1 cut(s) 1131
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 3 cut(s) 178, 956, 2706
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse8387I CCTGCAGG 1 cut(s) 1564
SspMI CTAG 6 cut(s) 248, 549, 1064, 2645, 2775, 3213
SstI GAGCTC 3 cut(s) 777, 921, 1020
StyI CCWWGG 4 cut(s) 14, 703, 1744, 3043
TaaI ACNGT 9 cut(s) 1159, 1217, 1317, 1361, 1734, 2277, 2498, 2550, 2801
TaiI ACGT 9 cut(s) 1520, 1628, 1655, 1962, 2268, 2325, 2352, 2388, 2494
TauI GCSGC 7 cut(s) 396, 2106, 2114, 2117, 2171, 2453, 2456
Tru1I TTAA 3 cut(s) 35, 351, 1956
Tru9I TTAA 3 cut(s) 35, 351, 1956
TscAI CASTG 5 cut(s) 372, 393, 1806, 2072, 2987
TseFI GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
TseI GCWGC 9 cut(s) 421, 828, 1060, 1140, 1417, 1503, 2061, 2189, 3149
Tsp45I GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
TspDTI ATGAA 5 cut(s) 96, 180, 974, 2823, 3152
TspGWI ACGGA 3 cut(s) 2417, 2793, 3232
TspRI CASTG 5 cut(s) 372, 393, 1806, 2072, 2987
Tth111I GACNNNGTC 1 cut(s) 2348
Van91I CCANNNNNTGG 3 cut(s) 217, 704, 940
XagI CCTNNNNNAGG 1 cut(s) 3186
XapI RAATTY 7 cut(s) 226, 265, 961, 1133, 1444, 3090, 3165
XceI RCATGY 2 cut(s) 1939, 2975
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XhoI CTCGAG 1 cut(s) 722
XmiI GTMKAC 1 cut(s) 2262
XmnI GAANNNNTTC 3 cut(s) 1360, 1856, 2423
XspI CTAG 6 cut(s) 248, 549, 1064, 2645, 2775, 3213
ZraI GACGTC 1 cut(s) 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.