Rh6BG033900

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000466
Physical Location & Seq
Forward (+)
5210413 .. 5211084
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG033900.1

Sequence Viewer

Length: 672 bp
ATGTTGGCTGGTTGCTCTAGTTCTACACTGTTGTCACCGAGGCATAGATTGAGGAGCGAAGCAGCAGCCCAGTTACAGGCTTGCCATTTCCAGCTACCATCAATGAGCACACAGAGACTCGACTTACCATGCAGCTTTCCTCGAAAAGACTCATCTCGCTCCAATGGCTCCCATTCAATTCCGAGGTCCGATTCAATTCCGAAGGAGATGGTGTCGCCGAGAAGGCGAGATACGCGATGGAAGTACAATGTGGCCAGCTTCCGGGTCGTAGGAAAGGACGGCGAGGTCGAACGGTTTTGCTCCGAGCTGGGCTTATCCCGCCCAGAAGACTTCGCTATACCCGTGGCGGCCCGGGAGTCCCTGAGGATCCGGTCCGGAAGGCCAGATACGCGATGGAAGTACAATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCTCCGAGCTGTGTGATAGACTAGAACGGAGAGGAATTAAAACCTTTATGGATGACCCCGATCTTCGTGTAGGAGATTCCATTAGCCCGTCTCTTGTTTCAGCAATTGAGGAGTCAAGATTTGCCATTGTCATTCTTTCACCCAACTATGCTTCTTCGCCATGGTGCTTGGATGAACTCCTCACGATTATTCGGTGCACCAAAGAGAAGGGGCAGCAGAGTTGA

Protein Analysis

223

Amino Acids

25.18

Weight (kDa)

9.47

Isoelectric Point (pI)

72.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 133 - 221 1.1e-31 TIR domain
TIR_2 PF13676 136 - 220 7.3e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccII CGCG 3 cut(s) 235, 391, 436
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 2 cut(s) 319, 347
AclWI GGATC 2 cut(s) 361, 374
AcoI YGGCCR 1 cut(s) 252
AfaI GTAC 2 cut(s) 245, 401
AfiI CCNNNNNNNGG 2 cut(s) 76, 261
AgsI TTSAA 2 cut(s) 177, 195
AluBI AGCT 5 cut(s) 94, 135, 258, 307, 457
AluI AGCT 5 cut(s) 94, 135, 258, 307, 457
Alw21I GWGCWC 2 cut(s) 110, 647
Alw26I GTCTC 2 cut(s) 109, 543
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 2 cut(s) 361, 374
Ama87I CYCGRG 1 cut(s) 351
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 4 cut(s) 252, 348, 380, 425
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 4 cut(s) 62, 65, 132, 661
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 186, 349, 372, 425
AsuC2I CCSGG 3 cut(s) 263, 352, 353
AsuHPI GGTGA 2 cut(s) 27, 579
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 2 cut(s) 186, 372
AxyI CCTNAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 647
BalI TGGCCA 1 cut(s) 254
BamHI GGATCC 1 cut(s) 366
BarI GAAGNNNNNNTAC 4 cut(s) 214, 246, 370, 402
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 110, 647
BbvI GCAGC 3 cut(s) 74, 77, 144
BccI CCATC 4 cut(s) 106, 202, 231, 387
BceAI ACGGC 1 cut(s) 295
BcnI CCSGG 3 cut(s) 263, 352, 353
BcoDI GTCTC 2 cut(s) 109, 543
BfaI CTAG 2 cut(s) 18, 470
BglI GCCNNNNNGGC 1 cut(s) 223
BisI GCNGC 5 cut(s) 63, 66, 133, 348, 662
BlsI GCNGC 5 cut(s) 64, 67, 134, 349, 663
Bme1390I CCNGG 3 cut(s) 263, 352, 353
Bme18I GGWCC 2 cut(s) 186, 372
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 4 cut(s) 186, 349, 372, 425
BmiI GGNNCC 2 cut(s) 169, 368
BmrFI CCNGG 3 cut(s) 263, 352, 353
BmrI ACTGGG 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 333
BpuMI CCSGG 3 cut(s) 263, 352, 353
BsaJI CCNNGG 5 cut(s) 38, 182, 342, 351, 608
BsaWI WCCGGW 2 cut(s) 369, 374
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 261
Bse1I ACTGG 1 cut(s) 70
Bse21I CCTNAGG 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 374
BseDI CCNNGG 5 cut(s) 38, 182, 342, 351, 608
BseGI GGATG 2 cut(s) 505, 625
BseLI CCNNNNNNNGG 2 cut(s) 76, 261
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 3 cut(s) 67, 572, 617
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 3 cut(s) 74, 77, 144
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 3 cut(s) 235, 391, 436
BshFI GGCC 4 cut(s) 254, 350, 382, 427
BsiHKAI GWGCWC 2 cut(s) 110, 647
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 4 cut(s) 262, 352, 370, 375
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 2 cut(s) 76, 261
BsmAI GTCTC 2 cut(s) 109, 543
BsmBI CGTCTC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 4 cut(s) 254, 350, 382, 427
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 110, 647
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 2 cut(s) 366, 508
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 2 cut(s) 319, 347
BspANI GGCC 4 cut(s) 254, 350, 382, 427
BspCNI CTCAG 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 374
BspFNI CGCG 3 cut(s) 235, 391, 436
BspLI GGNNCC 2 cut(s) 169, 368
BspPI GGATC 2 cut(s) 361, 374
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 5 cut(s) 38, 182, 342, 351, 608
BssMI GATC 2 cut(s) 366, 508
BssT1I CCWWGG 1 cut(s) 608
Bst4CI ACNGT 2 cut(s) 30, 294
BstC8I GCNNGC 2 cut(s) 82, 256
BstDEI CTNAG 2 cut(s) 362, 413
BstDSI CCRYGG 2 cut(s) 342, 608
BstF5I GGATG 2 cut(s) 505, 625
BstFNI CGCG 3 cut(s) 235, 391, 436
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 2 cut(s) 369, 511
BstMAI GTCTC 2 cut(s) 109, 543
BstMBI GATC 2 cut(s) 366, 508
BstMWI GCNNNNNNNGC 6 cut(s) 165, 223, 232, 318, 388, 433
BstSCI CCNGG 3 cut(s) 261, 350, 351
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 3 cut(s) 235, 391, 436
BstV1I GCAGC 3 cut(s) 74, 77, 144
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
Bsu36I CCTNAGG 1 cut(s) 362
BsuRI GGCC 4 cut(s) 254, 350, 382, 427
BtgI CCRYGG 2 cut(s) 342, 608
BtgZI GCGATG 2 cut(s) 250, 406
BtsCI GGATG 2 cut(s) 505, 625
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 82, 256
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 4 cut(s) 186, 349, 372, 425
Cfr9I CCCGGG 1 cut(s) 351
CpoI CGGWCCG 1 cut(s) 372
Csp6I GTAC 2 cut(s) 244, 400
CspI CGGWCCG 1 cut(s) 372
CviAII CATG 2 cut(s) 129, 609
CviQI GTAC 2 cut(s) 244, 400
DdeI CTNAG 2 cut(s) 362, 413
DpnI GATC 2 cut(s) 368, 510
DpnII GATC 2 cut(s) 366, 508
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 186, 372
Eco81I CCTNAGG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 608
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 2 cut(s) 132, 612
FaiI YATR 6 cut(s) 45, 130, 338, 497, 597, 610
FaqI GGGAC 1 cut(s) 343
FatI CATG 2 cut(s) 128, 608
FauI CCCGC 1 cut(s) 326
Fnu4HI GCNGC 5 cut(s) 63, 66, 133, 348, 662
FokI GGATG 2 cut(s) 512, 632
Fsp4HI GCNGC 5 cut(s) 63, 66, 133, 348, 662
FspBI CTAG 2 cut(s) 18, 470
GlaI GCGC 1 cut(s) 437
GluI GCNGC 5 cut(s) 63, 66, 133, 348, 662
GsaI CCCAGC 1 cut(s) 311
HaeIII GGCC 4 cut(s) 254, 350, 382, 427
HapII CCGG 4 cut(s) 262, 352, 370, 375
HhaI GCGC 1 cut(s) 438
Hin1II CATG 2 cut(s) 132, 612
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HinfI GANTC 6 cut(s) 117, 149, 191, 356, 524, 560
HpaII CCGG 4 cut(s) 262, 352, 370, 375
HphI GGTGA 2 cut(s) 27, 579
Hpy166II GTNNAC 1 cut(s) 645
Hpy188I TCNGA 6 cut(s) 183, 190, 201, 304, 422, 454
Hpy188III TCNNGA 3 cut(s) 375, 564, 631
Hpy8I GTNNAC 1 cut(s) 645
HpyAV CCTTC 4 cut(s) 196, 216, 372, 649
HpyCH4III ACNGT 2 cut(s) 30, 294
HpyCH4V TGCA 2 cut(s) 132, 645
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 223, 232, 318, 388, 433
HpyF3I CTNAG 2 cut(s) 362, 413
Hsp92II CATG 2 cut(s) 132, 612
HspAI GCGC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 374
Kzo9I GATC 2 cut(s) 366, 508
LmnI GCTCC 4 cut(s) 54, 164, 173, 305
Lsp1109I GCAGC 3 cut(s) 74, 77, 144
MaeI CTAG 2 cut(s) 18, 470
MaeIII GTNAC 2 cut(s) 33, 72
MalI GATC 2 cut(s) 368, 510
MboI GATC 2 cut(s) 366, 508
MboII GAAGA 3 cut(s) 338, 503, 594
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 110, 647
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 4 cut(s) 177, 195, 483, 552
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 4 cut(s) 111, 143, 365, 569
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 4 cut(s) 262, 352, 370, 375
MspR9I CCNGG 3 cut(s) 263, 352, 353
MunI CAATTG 1 cut(s) 552
MvnI CGCG 3 cut(s) 235, 391, 436
MwoI GCNNNNNNNGC 6 cut(s) 165, 223, 232, 318, 388, 433
NciI CCSGG 3 cut(s) 263, 352, 353
NcoI CCATGG 1 cut(s) 608
NdeII GATC 2 cut(s) 366, 508
NlaIII CATG 2 cut(s) 132, 612
NlaIV GGNNCC 2 cut(s) 169, 368
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 339
PfeI GAWTC 2 cut(s) 191, 524
PkrI GCNGC 5 cut(s) 64, 67, 134, 349, 663
PleI GAGTC 4 cut(s) 111, 143, 364, 568
PpsI GAGTC 4 cut(s) 111, 143, 364, 568
PspFI CCCAGC 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 169, 368
PspPI GGNCC 4 cut(s) 186, 349, 372, 425
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 2 cut(s) 245, 401
RsaNI GTAC 2 cut(s) 244, 400
Rsr2I CGGWCCG 1 cut(s) 372
RsrII CGGWCCG 1 cut(s) 372
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 5 cut(s) 63, 66, 133, 348, 662
Sau3AI GATC 2 cut(s) 366, 508
Sau96I GGNCC 4 cut(s) 186, 349, 372, 425
SchI GAGTC 4 cut(s) 111, 143, 365, 569
ScrFI CCNGG 3 cut(s) 263, 352, 353
SduI GDGCHC 2 cut(s) 110, 647
SetI ASST 9 cut(s) 96, 137, 188, 260, 288, 309, 452, 459, 494
SinI GGWCC 2 cut(s) 186, 372
SmaI CCCGGG 1 cut(s) 353
Sse9I AATT 4 cut(s) 177, 195, 483, 552
SsiI CCGC 2 cut(s) 319, 347
SspMI CTAG 2 cut(s) 18, 470
StyD4I CCNGG 3 cut(s) 261, 350, 351
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 2 cut(s) 30, 294
TaqI TCGA 3 cut(s) 120, 142, 288
TasI AATT 4 cut(s) 177, 195, 483, 552
TatI WGTACW 2 cut(s) 243, 399
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 2 cut(s) 191, 524
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 1 cut(s) 33
TseI GCWGC 4 cut(s) 62, 65, 132, 661
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 1 cut(s) 636
TspGWI ACGGA 1 cut(s) 490
TspMI CCCGGG 1 cut(s) 351
TspRI CASTG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 186, 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmaI CCCGGG 1 cut(s) 351
XspI CTAG 2 cut(s) 18, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.