Rw0G008150

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00301
OG0000466
Physical Location & Seq
Forward (+)
51768 .. 52767
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008150.1

Sequence Viewer

Length: 900 bp
ATGGTGCAATGGTTCGGTGTCATAGCCTGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACATGGTTGAAACCCCACAGTCAATATCAGAGGATAATCAAGAACCGGTTGACGTGGATGCCTTTGCATCTCTTCCTCCATCATCAGCTGAATCATCAGCTCCTAGGTGGAAGCATGATGTATTTTTGAGTTTCCGGGGTGTAGACACTCGCGGAGGTATTACGTTTGAAATATACGATCGACTGCAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAATGGCAATTAAAGAATCAAGGTTTGCAATTATTGCTCTCTCTCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGAAAGAAGACAACAATAGAATTCTTCCACTTTTTTATAATGTGGATCCTAGTGATGTACGAAATCAGAAGAGGAGTTTTGGAGATGCTTTCACTAAGCATGAAAAATCTGGGAAACACAAATCAGAAAAGGTGCAGCAGTGGAGAGATGCTTTAACAAAAGTGGCAAATTTCTCTGGATGGCATACACTGAATTATAAAACTGATAGAGAACTTGTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGATTGACTGAACCTGAGCTTAAAATGTGCATCGGAGGTTTTAAAGAATTTGCAGCAACAAAACAAGCCATTGATAAGGTCATGAACGCGCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGCGTTGGAAAGACGACAATGGTGA

Protein Analysis

299

Amino Acids

34.41

Weight (kDa)

6.16

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 80 - 253 2.7e-51 TIR domain
TIR_2 PF13676 83 - 194 9.3e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 528, 687
AccI GTMKAC 2 cut(s) 270, 864
AccII CGCG 2 cut(s) 279, 830
AciI CCGC 1 cut(s) 279
AclWI GGATC 2 cut(s) 530, 543
AcsI RAATTY 3 cut(s) 510, 658, 788
AdeI CACNNNGTG 1 cut(s) 456
AfaI GTAC 1 cut(s) 549
AfiI CCNNNNNNNGG 1 cut(s) 99
AflII CTTAAG 1 cut(s) 32
AgeI ACCGGT 1 cut(s) 172
AgsI TTSAA 3 cut(s) 137, 296, 359
AjiI CACGTC 1 cut(s) 181
AluBI AGCT 3 cut(s) 215, 227, 760
AluI AGCT 3 cut(s) 215, 227, 760
AlwI GGATC 2 cut(s) 530, 543
AoxI GGCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 625, 794
ApoI RAATTY 3 cut(s) 510, 658, 788
AsiGI ACCGGT 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 53
AspA2I CCTAGG 1 cut(s) 230
AspLEI GCGC 1 cut(s) 832
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 79
AvrII CCTAGG 1 cut(s) 230
BamHI GGATCC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 2 cut(s) 637, 806
BccI CCATC 2 cut(s) 214, 663
BcnI CCSGG 1 cut(s) 263
BfaI CTAG 2 cut(s) 231, 540
BfrI CTTAAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 626, 795
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 2 cut(s) 627, 796
Bme1390I CCNGG 1 cut(s) 263
BmgBI CACGTC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 6 cut(s) 175, 203, 565, 628, 700, 780
BpiI GAAGAC 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 230, 262
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 230, 262
BseGI GGATG 5 cut(s) 190, 349, 373, 674, 715
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 1 cut(s) 747
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 577
BseXI GCAGC 2 cut(s) 637, 806
BsgI GTGCAG 1 cut(s) 644
Bsh1236I CGCG 2 cut(s) 279, 830
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 1 cut(s) 848
BshTI ACCGGT 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 307
BsiSI CCGG 2 cut(s) 173, 262
BslI CCNNNNNNNGG 1 cut(s) 99
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 1 cut(s) 848
Bsp143I GATC 2 cut(s) 304, 535
BspACI CCGC 1 cut(s) 279
BspANI GGCC 1 cut(s) 848
BspCNI CTCAG 1 cut(s) 748
BspFNI CGCG 2 cut(s) 279, 830
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 1 cut(s) 537
BspPI GGATC 2 cut(s) 530, 543
BspTI CTTAAG 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 172
BsrI ACTGG 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 230, 262
BssMI GATC 2 cut(s) 304, 535
BssT1I CCWWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 4 cut(s) 107, 204, 451, 554
BstAFI CTTAAG 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 422
BstDEI CTNAG 3 cut(s) 470, 585, 756
BstF5I GGATG 5 cut(s) 190, 349, 373, 674, 715
BstFNI CGCG 2 cut(s) 279, 830
BstHHI GCGC 1 cut(s) 832
BstKTI GATC 2 cut(s) 307, 538
BstMBI GATC 2 cut(s) 304, 535
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 261
BstUI CGCG 2 cut(s) 279, 830
BstV1I GCAGC 2 cut(s) 637, 806
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BsuRI GGCC 1 cut(s) 848
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 5 cut(s) 190, 349, 373, 674, 715
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 2 cut(s) 635, 677
CciI TCATGA 1 cut(s) 822
CfoI GCGC 1 cut(s) 832
Cfr10I RCCGGY 1 cut(s) 172
Csp6I GTAC 1 cut(s) 548
CspAI ACCGGT 1 cut(s) 172
CspCI CAANNNNNGTGG 2 cut(s) 838, 873
CviAII CATG 7 cut(s) 78, 130, 242, 340, 490, 590, 823
CviJI RGCY 7 cut(s) 26, 92, 215, 227, 760, 809, 848
CviKI_1 RGCY 7 cut(s) 26, 92, 215, 227, 760, 809, 848
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 3 cut(s) 470, 585, 756
DpnI GATC 2 cut(s) 306, 537
DpnII GATC 2 cut(s) 304, 535
DraI TTTAAA 1 cut(s) 784
DraIII CACNNNGTG 1 cut(s) 456
Eam1104I CTCTTC 4 cut(s) 107, 204, 451, 554
EarI CTCTTC 4 cut(s) 107, 204, 451, 554
Eco130I CCWWGG 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 510
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 7 cut(s) 81, 133, 245, 343, 493, 593, 826
FatI CATG 7 cut(s) 77, 129, 241, 339, 489, 589, 822
FblI GTMKAC 2 cut(s) 270, 864
Fnu4HI GCNGC 2 cut(s) 626, 795
FokI GGATG 5 cut(s) 197, 356, 380, 681, 722
Fsp4HI GCNGC 2 cut(s) 626, 795
FspBI CTAG 2 cut(s) 231, 540
GlaI GCGC 1 cut(s) 831
GluI GCNGC 2 cut(s) 626, 795
HaeIII GGCC 1 cut(s) 848
HapII CCGG 2 cut(s) 173, 262
HhaI GCGC 1 cut(s) 832
Hin1II CATG 7 cut(s) 81, 133, 245, 343, 493, 593, 826
Hin6I GCGC 1 cut(s) 830
HinP1I GCGC 1 cut(s) 830
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 5 cut(s) 218, 327, 404, 722, 861
HpaII CCGG 2 cut(s) 173, 262
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 3 cut(s) 178, 271, 865
Hpy188I TCNGA 4 cut(s) 157, 558, 616, 776
Hpy188III TCNNGA 3 cut(s) 167, 666, 823
Hpy8I GTNNAC 3 cut(s) 178, 271, 865
HpyAV CCTTC 1 cut(s) 365
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 3 cut(s) 84, 180, 290
HpyCH4V TGCA 9 cut(s) 7, 194, 313, 416, 489, 625, 732, 771, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 3 cut(s) 470, 585, 756
HpySE526I ACGT 3 cut(s) 84, 180, 290
Hsp92II CATG 7 cut(s) 81, 133, 245, 343, 493, 593, 826
HspAI GCGC 1 cut(s) 830
Kzo9I GATC 2 cut(s) 304, 535
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 8 cut(s) 40, 106, 186, 275, 304, 585, 651, 768
Lsp1109I GCAGC 2 cut(s) 637, 806
LweI GCATC 6 cut(s) 175, 203, 565, 628, 700, 780
MaeI CTAG 2 cut(s) 231, 540
MaeII ACGT 3 cut(s) 84, 180, 290
MaeIII GTNAC 2 cut(s) 73, 125
MalI GATC 2 cut(s) 306, 537
MboI GATC 2 cut(s) 304, 535
MboII GAAGA 7 cut(s) 58, 124, 191, 438, 506, 509, 571
MflI RGATCY 1 cut(s) 535
MluCI AATT 8 cut(s) 49, 396, 417, 510, 658, 682, 737, 788
MlyI GAGTC 1 cut(s) 870
MmeI TCCRAC 1 cut(s) 861
Mph1103I ATGCAT 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 5 cut(s) 33, 399, 644, 762, 783
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 2 cut(s) 173, 262
MspR9I CCNGG 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 491
MvnI CGCG 2 cut(s) 279, 830
MwoI GCNNNNNNNGC 1 cut(s) 422
NciI CCSGG 1 cut(s) 263
NdeII GATC 2 cut(s) 304, 535
NlaIII CATG 7 cut(s) 81, 133, 245, 343, 493, 593, 826
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 1 cut(s) 73
NsiI ATGCAT 1 cut(s) 491
PagI TCATGA 1 cut(s) 822
PctI GAATGC 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 4 cut(s) 218, 327, 404, 722
PinAI ACCGGT 1 cut(s) 172
PkrI GCNGC 2 cut(s) 627, 796
Ple19I CGATCG 1 cut(s) 307
PleI GAGTC 1 cut(s) 869
PpsI GAGTC 1 cut(s) 869
PsiI TTATAA 2 cut(s) 528, 687
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 1 cut(s) 535
PvuI CGATCG 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 5 cut(s) 33, 399, 644, 762, 783
SatI GCNGC 2 cut(s) 626, 795
Sau3AI GATC 2 cut(s) 304, 535
SchI GAGTC 1 cut(s) 870
ScrFI CCNGG 1 cut(s) 263
SfaNI GCATC 6 cut(s) 175, 203, 565, 628, 700, 780
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 8 cut(s) 49, 396, 417, 510, 658, 682, 737, 788
SsiI CCGC 1 cut(s) 279
SspMI CTAG 2 cut(s) 231, 540
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 3 cut(s) 87, 183, 293
TaqI TCGA 1 cut(s) 307
TasI AATT 8 cut(s) 49, 396, 417, 510, 658, 682, 737, 788
TfiI GAWTC 4 cut(s) 218, 327, 404, 722
Tru1I TTAA 5 cut(s) 33, 399, 644, 762, 783
Tru9I TTAA 5 cut(s) 33, 399, 644, 762, 783
TscAI CASTG 2 cut(s) 635, 684
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 625, 794
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 5 cut(s) 46, 328, 506, 606, 839
TspGWI ACGGA 1 cut(s) 882
TspRI CASTG 2 cut(s) 635, 684
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 3 cut(s) 510, 658, 788
XcmI CCANNNNNNNNNTGG 2 cut(s) 388, 457
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 2 cut(s) 270, 864
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 231, 540
Zsp2I ATGCAT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.