RLG00000030327

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000466
Physical Location & Seq
Reverse (-)
59646599 .. 59647514
916 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030327

Sequence Viewer

Length: 738 bp
ATGGCTATGGATTTCTACAGTAGCAGACCTCTTCAGTCATCTTCGGATCCTTTTACGGATGAGCTCATGGAAGCACTGGAACCTTTTATGAAAAGTGCGTCTCCACCGCCACCTTCTTCTTCTTCTTTCTCCCTTCGATCCCTACTCCCCAGCCCAATTTGTACCAAGACGGTGGCTCAACCTTTTCCATGGGTTTCGAGCAGCAAGATCCAGGCCCAGATCCAGCTCCAGAATCAACCCCAGATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGAGAAGATAAGCGCAATGTTTTTACCGGTCTTCTGTATTCTGAACTAATTGGGAAAGGAATTCTGACGTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCTTGCGGCCCGAACTTTTAGGTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACTGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAAATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTCCCCGTCTTCTCCTGGGTGGATACTTCTGATGTGCGGCACCAAACGCGAAGTTTTGAGCTCAAATGGGAACACCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGAGGACAGACTAAATTCCTGGAGAGCTGCTTTGACAGAGCCAAGAATTATCCATACGAATCAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

246

Amino Acids

28.05

Weight (kDa)

5.36

Isoelectric Point (pI)

63.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 92 - 236 2.2e-37 TIR domain
TIR_2 PF13676 95 - 187 7e-13 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 585
AccII CGCG 1 cut(s) 595
AciI CCGC 3 cut(s) 107, 409, 583
AclWI GGATC 5 cut(s) 41, 54, 132, 202, 214
AcsI RAATTY 2 cut(s) 360, 682
AcuI CTGAAG 1 cut(s) 17
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 1 cut(s) 163
AgeI ACCGGT 1 cut(s) 326
AgsI TTSAA 1 cut(s) 518
AjnI CCWGG 3 cut(s) 210, 560, 686
AluBI AGCT 5 cut(s) 64, 226, 388, 607, 695
AluI AGCT 5 cut(s) 64, 226, 388, 607, 695
Alw21I GWGCWC 2 cut(s) 66, 609
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 5 cut(s) 41, 54, 132, 202, 214
AoxI GGCC 3 cut(s) 213, 410, 535
ApeKI GCWGC 2 cut(s) 201, 695
ApoI RAATTY 2 cut(s) 360, 682
AsiGI ACCGGT 1 cut(s) 326
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 3 cut(s) 214, 411, 535
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 585
BanII GRGCYC 2 cut(s) 66, 609
BbsI GAAGAC 2 cut(s) 323, 547
Bbv12I GWGCWC 2 cut(s) 66, 609
BbvI GCAGC 2 cut(s) 213, 682
BccI CCATC 2 cut(s) 238, 526
BciT130I CCWGG 3 cut(s) 212, 562, 688
BciVI GTATCC 1 cut(s) 562
BcoDI GTCTC 1 cut(s) 105
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 16
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 1 cut(s) 443
BisI GCNGC 4 cut(s) 202, 410, 584, 696
BlsI GCNGC 4 cut(s) 203, 411, 585, 697
Bme1390I CCNGG 3 cut(s) 212, 562, 688
BmgT120I GGNCC 3 cut(s) 214, 411, 535
BmiI GGNNCC 3 cut(s) 48, 81, 587
BmrFI CCNGG 3 cut(s) 212, 562, 688
BpiI GAAGAC 2 cut(s) 323, 547
BpmI CTGGAG 2 cut(s) 212, 709
BpuEI CTTGAG 1 cut(s) 410
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 188, 561
BsaWI WCCGGW 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 322
BseBI CCWGG 3 cut(s) 212, 562, 688
BseDI CCNNGG 2 cut(s) 188, 561
BseGI GGATG 3 cut(s) 64, 382, 502
BseMI GCAATG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 213, 682
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 3 cut(s) 215, 412, 537
BshNI GGYRCC 1 cut(s) 585
BshTI ACCGGT 1 cut(s) 326
BsiHKAI GWGCWC 2 cut(s) 66, 609
BsiSI CCGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 105
BsmBI CGTCTC 1 cut(s) 105
BsnI GGCC 3 cut(s) 215, 412, 537
Bsp1286I GDGCHC 2 cut(s) 66, 609
Bsp143I GATC 5 cut(s) 46, 137, 207, 219, 443
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 3 cut(s) 107, 409, 583
BspANI GGCC 3 cut(s) 215, 412, 537
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 3 cut(s) 48, 81, 587
BspPI GGATC 5 cut(s) 41, 54, 132, 202, 214
BspT107I GGYRCC 1 cut(s) 585
BsrDI GCAATG 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 188, 561
BssMI GATC 5 cut(s) 46, 137, 207, 219, 443
BssNI GRCGYC 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 212, 562, 688
Bst4CI ACNGT 2 cut(s) 20, 172
Bst6I CTCTTC 2 cut(s) 36, 262
BstACI GRCGYC 1 cut(s) 248
BstDEI CTNAG 1 cut(s) 290
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 3 cut(s) 64, 382, 502
BstFNI CGCG 1 cut(s) 595
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 5 cut(s) 49, 140, 210, 222, 446
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 5 cut(s) 46, 137, 207, 219, 443
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNI CCWGG 3 cut(s) 212, 562, 688
BstSCI CCNGG 3 cut(s) 210, 560, 686
BstSFI CTRYAG 1 cut(s) 16
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 213, 682
BstV2I GAAGAC 2 cut(s) 323, 547
BstX2I RGATCY 4 cut(s) 46, 207, 219, 443
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 4 cut(s) 46, 207, 219, 443
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 3 cut(s) 215, 412, 537
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 3 cut(s) 64, 382, 502
BtsIMutI CAGTG 2 cut(s) 74, 464
CfoI GCGC 1 cut(s) 315
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 214, 411, 535
CseI GACGC 3 cut(s) 87, 237, 389
Csp6I GTAC 1 cut(s) 162
CspAI ACCGGT 1 cut(s) 326
CviAII CATG 5 cut(s) 67, 189, 486, 523, 647
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 1 cut(s) 290
DpnI GATC 5 cut(s) 48, 139, 209, 221, 445
DpnII GATC 5 cut(s) 46, 137, 207, 219, 443
Eam1104I CTCTTC 2 cut(s) 36, 262
EarI CTCTTC 2 cut(s) 36, 262
Ecl136II GAGCTC 2 cut(s) 64, 607
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 2 cut(s) 66, 609
Eco53kI GAGCTC 2 cut(s) 64, 607
Eco57I CTGAAG 1 cut(s) 17
EcoICRI GAGCTC 2 cut(s) 64, 607
EcoRI GAATTC 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 210, 560, 686
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 1 cut(s) 524
EcoT38I GRGCYC 2 cut(s) 66, 609
ErhI CCWWGG 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 5 cut(s) 70, 192, 489, 526, 650
FatI CATG 5 cut(s) 66, 188, 485, 522, 646
Fnu4HI GCNGC 4 cut(s) 202, 410, 584, 696
FokI GGATG 3 cut(s) 71, 389, 509
FriOI GRGCYC 2 cut(s) 66, 609
Fsp4HI GCNGC 4 cut(s) 202, 410, 584, 696
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 314
GluI GCNGC 4 cut(s) 202, 410, 584, 696
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 2 cut(s) 212, 709
HaeIII GGCC 3 cut(s) 215, 412, 537
HapII CCGG 1 cut(s) 327
HgaI GACGC 3 cut(s) 87, 237, 389
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 5 cut(s) 70, 192, 489, 526, 650
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HinfI GANTC 3 cut(s) 232, 437, 727
HpaII CCGG 1 cut(s) 327
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 4 cut(s) 46, 343, 366, 577
Hpy188III TCNNGA 2 cut(s) 229, 441
Hpy8I GTNNAC 1 cut(s) 252
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 123, 143
HpyCH4III ACNGT 2 cut(s) 20, 172
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 2 cut(s) 428, 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 1 cut(s) 368
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 5 cut(s) 70, 192, 489, 526, 650
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 5 cut(s) 46, 137, 207, 219, 443
LmnI GCTCC 1 cut(s) 231
Lsp1109I GCAGC 2 cut(s) 213, 682
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 368
MalI GATC 5 cut(s) 48, 139, 209, 221, 445
MboI GATC 5 cut(s) 46, 137, 207, 219, 443
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 4 cut(s) 46, 207, 219, 443
MhlI GDGCHC 2 cut(s) 66, 609
MluCI AATT 7 cut(s) 156, 348, 360, 429, 510, 682, 714
MlyI GAGTC 1 cut(s) 446
MnlI CCTC 5 cut(s) 39, 265, 427, 557, 664
Mph1103I ATGCAT 1 cut(s) 524
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 3 cut(s) 212, 562, 688
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 3 cut(s) 212, 562, 688
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 1 cut(s) 592
NcoI CCATGG 1 cut(s) 188
NdeII GATC 5 cut(s) 46, 137, 207, 219, 443
NlaIII CATG 5 cut(s) 70, 192, 489, 526, 650
NlaIV GGNNCC 3 cut(s) 48, 81, 587
NsiI ATGCAT 1 cut(s) 524
PfeI GAWTC 2 cut(s) 232, 727
PfoI TCCNGGA 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 326
PkrI GCNGC 4 cut(s) 203, 411, 585, 697
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
Psp124BI GAGCTC 2 cut(s) 66, 609
Psp6I CCWGG 3 cut(s) 210, 560, 686
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 210, 560, 686
PspN4I GGNNCC 3 cut(s) 48, 81, 587
PspPI GGNCC 3 cut(s) 214, 411, 535
PsuI RGATCY 4 cut(s) 46, 207, 219, 443
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SacI GAGCTC 2 cut(s) 66, 609
SatI GCNGC 4 cut(s) 202, 410, 584, 696
Sau3AI GATC 5 cut(s) 46, 137, 207, 219, 443
Sau96I GGNCC 3 cut(s) 214, 411, 535
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 3 cut(s) 212, 562, 688
SduI GDGCHC 2 cut(s) 66, 609
SfcI CTRYAG 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 7 cut(s) 156, 348, 360, 429, 510, 682, 714
SsiI CCGC 3 cut(s) 107, 409, 583
SspMI CTAG 1 cut(s) 441
SstI GAGCTC 2 cut(s) 66, 609
StyD4I CCNGG 3 cut(s) 210, 560, 686
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 20, 172
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 136, 197
TasI AATT 7 cut(s) 156, 348, 360, 429, 510, 682, 714
TauI GCSGC 2 cut(s) 412, 586
TfiI GAWTC 2 cut(s) 232, 727
TscAI CASTG 2 cut(s) 81, 471
TseI GCWGC 2 cut(s) 201, 695
TspDTI ATGAA 4 cut(s) 104, 513, 539, 663
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 2 cut(s) 81, 471
XapI RAATTY 2 cut(s) 360, 682
XbaI TCTAGA 1 cut(s) 440
XspI CTAG 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.