Rroxscaffold_4G00327070

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000466
Physical Location & Seq
Forward (+)
59508161 .. 59508544
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327070.1

Sequence Viewer

Length: 384 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTATAAGTTTCGGCGGGATCGATACCCGCGATACTTTTACCAATTATCTGTATTCTGCACTAAATGCGAGAGGAATTCTCACCTTTACGGATGACCGAGAGCTTGAGAAAGGAAAATCCATTAGACCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACGGAACTACGCTCGTTCGTCATGGTGCTTGGATGAACTGGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTCCCCATCTTCTACGGGGTGGATCCTTCGATGTGCGGCACCAAAGGGGACATTTTGAGCTCAAAAGGAAAACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

127

Amino Acids

14.33

Weight (kDa)

8.81

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 117 1.1e-39 TIR domain
TIR_2 PF13676 14 - 107 2.2e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 341
AccII CGCG 1 cut(s) 72
AciI CCGC 3 cut(s) 57, 70, 339
AclWI GGATC 3 cut(s) 68, 320, 333
AcsI RAATTY 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 5
AgsI TTSAA 1 cut(s) 275
AluBI AGCT 3 cut(s) 145, 182, 363
AluI AGCT 3 cut(s) 145, 182, 363
Alw21I GWGCWC 1 cut(s) 365
AlwI GGATC 3 cut(s) 68, 320, 333
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 115
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 169
BccI CCATC 2 cut(s) 283, 317
BfaI CTAG 1 cut(s) 198
BglII AGATCT 1 cut(s) 200
BisI GCNGC 2 cut(s) 183, 340
BlsI GCNGC 2 cut(s) 184, 341
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 2 cut(s) 327, 343
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpuEI CTTGAG 1 cut(s) 167
Bsa29I ATCGAT 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bse1I ACTGG 1 cut(s) 264
BseCI ATCGAT 1 cut(s) 63
BseGI GGATG 2 cut(s) 139, 259
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 169
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 365
BslFI GGGAC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 3 cut(s) 60, 200, 325
BspACI CCGC 3 cut(s) 57, 70, 339
BspANI GGCC 1 cut(s) 294
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 327, 343
BspPI GGATC 3 cut(s) 68, 320, 333
BspT107I GGYRCC 1 cut(s) 341
BsrI ACTGG 1 cut(s) 264
BssMI GATC 3 cut(s) 60, 200, 325
BssNI GRCGYC 1 cut(s) 5
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 107
BstF5I GGATG 2 cut(s) 139, 259
BstFNI CGCG 1 cut(s) 72
BstKTI GATC 3 cut(s) 63, 203, 328
BstMBI GATC 3 cut(s) 60, 200, 325
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 169
BstX2I RGATCY 2 cut(s) 200, 325
BstYI RGATCY 2 cut(s) 200, 325
Bsu15I ATCGAT 1 cut(s) 63
BsuRI GGCC 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 63
BtsCI GGATG 2 cut(s) 139, 259
Cfr13I GGNCC 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 63
CviAII CATG 2 cut(s) 243, 280
CviJI RGCY 4 cut(s) 145, 182, 294, 363
CviKI_1 RGCY 4 cut(s) 145, 182, 294, 363
DpnI GATC 3 cut(s) 62, 202, 327
DpnII GATC 3 cut(s) 60, 200, 325
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 365
FaeI CATG 2 cut(s) 246, 283
FaiI YATR 3 cut(s) 47, 244, 281
FaqI GGGAC 1 cut(s) 365
FatI CATG 2 cut(s) 242, 279
FauI CCCGC 2 cut(s) 50, 77
Fnu4HI GCNGC 2 cut(s) 183, 340
FokI GGATG 2 cut(s) 146, 266
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 2 cut(s) 183, 340
FspBI CTAG 1 cut(s) 198
GluI GCNGC 2 cut(s) 183, 340
HaeIII GGCC 1 cut(s) 294
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 246, 283
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 1 cut(s) 194
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 1 cut(s) 9
Hpy188III TCNNGA 2 cut(s) 198, 265
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 101, 185, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 246, 283
Kzo9I GATC 3 cut(s) 60, 200, 325
LpnPI CCDG 2 cut(s) 183, 245
Lsp1109I GCAGC 1 cut(s) 169
MaeI CTAG 1 cut(s) 198
MalI GATC 3 cut(s) 62, 202, 327
MboI GATC 3 cut(s) 60, 200, 325
MboII GAAGA 2 cut(s) 6, 304
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 2 cut(s) 200, 325
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 3 cut(s) 85, 117, 186
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 4 cut(s) 22, 107, 184, 314
Mph1103I ATGCAT 1 cut(s) 281
MunI CAATTG 1 cut(s) 186
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 3 cut(s) 60, 200, 325
NlaIII CATG 2 cut(s) 246, 283
NlaIV GGNNCC 2 cut(s) 327, 343
NsiI ATGCAT 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 69
PkrI GCNGC 2 cut(s) 184, 341
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PsiI TTATAA 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 365
PspN4I GGNNCC 2 cut(s) 327, 343
PspPI GGNCC 1 cut(s) 292
PsuI RGATCY 2 cut(s) 200, 325
SacI GAGCTC 1 cut(s) 365
SatI GCNGC 2 cut(s) 183, 340
Sau3AI GATC 3 cut(s) 60, 200, 325
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 1 cut(s) 365
SetI ASST 6 cut(s) 14, 128, 147, 172, 184, 365
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 3 cut(s) 85, 117, 186
SsiI CCGC 3 cut(s) 57, 70, 339
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 1 cut(s) 365
TaqI TCGA 2 cut(s) 63, 332
TaqII GACCGA 1 cut(s) 153
TasI AATT 3 cut(s) 85, 117, 186
TauI GCSGC 1 cut(s) 342
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 270, 296
TspGWI ACGGA 2 cut(s) 146, 238
XapI RAATTY 1 cut(s) 117
XbaI TCTAGA 1 cut(s) 197
XspI CTAG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.