RLG00000036833

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000466
Physical Location & Seq
Reverse (-)
85471278 .. 85472138
861 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036833

Sequence Viewer

Length: 750 bp
ATGGAGTTATTCTTCCAGATTTTGTTGGCGATCGTTGTGGTAATTGTGACATGGATCGGTGCCATAGCTACTATTAAGTGGATATGGACTCTACTGAAGCATTGGTTCCGTTACATGATTGAAAGGCGTGAACCAATATCTCAGGATAATCAAGAATTGATCGACATCGCTACTGAGCCCGAGAGAGAAGAAGAAGAAAAGATGTGGGATCCCAGTGACTCTCCTGATCAGGGAAACAATGGCACCACCTCGGCCGCCGCACCGCTTGCTTCTTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTGTTTTTGAGTTTTAGGGGTCCTGACACTCGCAAGGGTATCACATTTGAACTATACGATCGACTGCAGAGGAGGGGAATTAAAGCATTCATGGATGATCGAGATCTTGAAGTAGGGGATGTTATTTCTCCAACTCTCTTAAGAGCAATTAGAGAATCAAGGTTTGCAATCGTTATTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACAACAGTAGAATCCTGCCACTCTTTTATCAGGTTGATCCTACTGATGTTCGATATCAGAAGAGGAGTTTTGAAGAAGCTTTCACTAGGCATGAAACCTCTGGGCGACATGAATCAGACAAGGTGGAAAAGTGGAAAGCTGCTTTAAACAAAGTGGCCAATATCTCTGGGTGGAATACAAATGATCATAAGTAA

Protein Analysis

250

Amino Acids

28.97

Weight (kDa)

5.11

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 101 - 246 8.5e-47 TIR domain
TIR_2 PF13676 104 - 199 3e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 59, 242
AciI CCGC 3 cut(s) 255, 258, 263
AclWI GGATC 4 cut(s) 62, 203, 216, 587
AcoI YGGCCR 2 cut(s) 252, 711
AcuI CTGAAG 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 230
AflII CTTAAG 1 cut(s) 448
AgsI TTSAA 4 cut(s) 122, 359, 419, 629
AluBI AGCT 4 cut(s) 68, 290, 635, 695
AluI AGCT 4 cut(s) 68, 290, 635, 695
AlwI GGATC 4 cut(s) 62, 203, 216, 587
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 2 cut(s) 252, 711
ApeKI GCWGC 1 cut(s) 695
AspS9I GGNCC 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 713
BamHI GGATCC 1 cut(s) 208
BanI GGYRCC 2 cut(s) 59, 242
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 682
BclI TGATCA 2 cut(s) 226, 739
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 374
BfrI CTTAAG 1 cut(s) 448
BglII AGATCT 1 cut(s) 412
BisI GCNGC 3 cut(s) 255, 258, 696
BlsI GCNGC 3 cut(s) 256, 259, 697
Bme18I GGWCC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 5 cut(s) 61, 107, 210, 244, 330
BmrI ACTGGG 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 561
BsaBI GATNNNNATC 2 cut(s) 164, 411
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 213
Bse8I GATNNNNATC 2 cut(s) 164, 411
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 2 cut(s) 409, 433
BseJI GATNNNNATC 2 cut(s) 164, 411
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 2 cut(s) 155, 165
BseNI ACTGG 1 cut(s) 213
BseRI GAGGAG 2 cut(s) 394, 634
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 1 cut(s) 682
Bsh1285I CGRYCG 3 cut(s) 33, 255, 370
BshFI GGCC 2 cut(s) 254, 713
BshNI GGYRCC 2 cut(s) 59, 242
BsiEI CGRYCG 3 cut(s) 33, 255, 370
BsiHKCI CYCGRG 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 230
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 2 cut(s) 254, 713
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 180
BspACI CCGC 3 cut(s) 255, 258, 263
BspANI GGCC 2 cut(s) 254, 713
BspCNI CTCAG 2 cut(s) 154, 166
BspLI GGNNCC 5 cut(s) 61, 107, 210, 244, 330
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 4 cut(s) 62, 203, 216, 587
BspT107I GGYRCC 2 cut(s) 59, 242
BspTI CTTAAG 1 cut(s) 448
BsrI ACTGG 1 cut(s) 213
BssECI CCNNGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 563
Bst6I CTCTTC 1 cut(s) 611
BstAFI CTTAAG 1 cut(s) 448
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 3 cut(s) 141, 174, 530
BstF5I GGATG 2 cut(s) 409, 433
BstMCI CGRYCG 3 cut(s) 33, 255, 370
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 682
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 208, 412
BstYI RGATCY 2 cut(s) 208, 412
BstZI CGGCCG 1 cut(s) 252
BsuRI GGCC 2 cut(s) 254, 713
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 2 cut(s) 409, 433
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 329
CviAII CATG 6 cut(s) 51, 115, 400, 550, 647, 665
CviJI RGCY 7 cut(s) 68, 178, 254, 290, 635, 695, 713
CviKI_1 RGCY 7 cut(s) 68, 178, 254, 290, 635, 695, 713
DdeI CTNAG 3 cut(s) 141, 174, 530
DraI TTTAAA 1 cut(s) 702
EaeI YGGCCR 2 cut(s) 252, 711
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
EclXI CGGCCG 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 329
Eco52I CGGCCG 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 329
EcoRV GATATC 1 cut(s) 611
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 180
FaeI CATG 6 cut(s) 54, 118, 403, 553, 650, 668
FatI CATG 6 cut(s) 50, 114, 399, 549, 646, 664
FbaI TGATCA 2 cut(s) 226, 739
Fnu4HI GCNGC 3 cut(s) 255, 258, 696
FokI GGATG 2 cut(s) 416, 440
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 255, 258, 696
FspBI CTAG 1 cut(s) 642
GluI GCNGC 3 cut(s) 255, 258, 696
HaeIII GGCC 2 cut(s) 254, 713
Hin1II CATG 6 cut(s) 54, 118, 403, 553, 650, 668
HindIII AAGCTT 1 cut(s) 633
HinfI GANTC 5 cut(s) 88, 218, 464, 567, 668
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 2 cut(s) 615, 673
Hpy188III TCNNGA 7 cut(s) 16, 143, 152, 224, 332, 410, 416
Hpy8I GTNNAC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4V TGCA 3 cut(s) 376, 476, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 3 cut(s) 141, 174, 530
Hsp92II CATG 6 cut(s) 54, 118, 403, 553, 650, 668
Ksp22I TGATCA 2 cut(s) 226, 739
Lsp1109I GCAGC 1 cut(s) 682
MaeI CTAG 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 46, 110, 215
MflI RGATCY 2 cut(s) 208, 412
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 713
MluCI AATT 5 cut(s) 42, 155, 387, 456, 496
MluNI TGGCCA 1 cut(s) 713
MlyI GAGTC 2 cut(s) 82, 212
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 6 cut(s) 259, 372, 375, 517, 612, 664
Mox20I TGGCCA 1 cut(s) 713
Mph1103I ATGCAT 1 cut(s) 551
MscI TGGCCA 1 cut(s) 713
MseI TTAA 4 cut(s) 75, 390, 449, 701
Msp20I TGGCCA 1 cut(s) 713
MspA1I CMGCKG 1 cut(s) 290
MspCI CTTAAG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 266
NlaIII CATG 6 cut(s) 54, 118, 403, 553, 650, 668
NlaIV GGNNCC 5 cut(s) 61, 107, 210, 244, 330
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 2 cut(s) 46, 215
NsiI ATGCAT 1 cut(s) 551
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 3 cut(s) 464, 567, 668
PkrI GCNGC 3 cut(s) 256, 259, 697
Ple19I CGATCG 2 cut(s) 33, 370
PleI GAGTC 2 cut(s) 82, 212
PpsI GAGTC 2 cut(s) 82, 212
PpuMI RGGWCCY 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 329
PspN4I GGNNCC 5 cut(s) 61, 107, 210, 244, 330
PspPI GGNCC 1 cut(s) 329
PspPPI RGGWCCY 1 cut(s) 329
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 208, 412
PvuI CGATCG 2 cut(s) 33, 370
PvuII CAGCTG 1 cut(s) 290
SaqAI TTAA 4 cut(s) 75, 390, 449, 701
SatI GCNGC 3 cut(s) 255, 258, 696
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 82, 212
SduI GDGCHC 1 cut(s) 180
SetI ASST 9 cut(s) 70, 251, 292, 473, 591, 637, 656, 681, 697
SfcI CTRYAG 1 cut(s) 374
SinI GGWCC 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
Sse9I AATT 5 cut(s) 42, 155, 387, 456, 496
SsiI CCGC 3 cut(s) 255, 258, 263
SspMI CTAG 1 cut(s) 642
TaaI ACNGT 1 cut(s) 563
TaqI TCGA 4 cut(s) 162, 370, 409, 607
TasI AATT 5 cut(s) 42, 155, 387, 456, 496
TauI GCSGC 2 cut(s) 257, 260
TfiI GAWTC 3 cut(s) 464, 567, 668
Tru1I TTAA 4 cut(s) 75, 390, 449, 701
Tru9I TTAA 4 cut(s) 75, 390, 449, 701
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 46, 215
TseI GCWGC 1 cut(s) 695
Tsp45I GTSAC 2 cut(s) 46, 215
TspDTI ATGAA 3 cut(s) 388, 663, 681
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 329
XspI CTAG 1 cut(s) 642
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.