Rorug01G0038900

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000466
Physical Location & Seq
Forward (+)
6387469 .. 6393406
5938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0038900.1

Sequence Viewer

Length: 4071 bp
ATGGTAGCGGAGGTCTTCCTCGGTGCATTCCTTCAGTTACTGCTCGACAGGTTAACTCCTCATCACGATTTGCTCAACTTGGCGCGCCTGCATGGCGTCGACAAGAAACTCAAGAAATGGAGTGCAGCGCTGTCTGCAATCGGAGCGGTGCTGCAGGACGCGGAGGACAAGCAACTGACGAGCGAGGCTGTGAAGCTATGGCTGGATGAACTCAAACACTTGGCTTTTGACATGGATGACATCTTGGACACCTTTTCCACCGAGCTGTTGCGGCGCAACGTGATGAATCAGCAACATGCGTGGCTTACGAGTAAGGTACGGGGAGCTAAGTTCAATTTCAATATGAATTCGGAGATCAAGGAGATTAGTGATAGGTTGGTAGAGATATCTGAAAGAAAGGACTTGCTTGGGTTGAATTATGTTGTGAATAAATGTAGTCGGGTATGGCAAAGGCCGCCGAGTTCATGTGTGTTAGATGGGCCTGTGGTTGGTAGGGATGATGATAAAAGGAAGATTGTTGAGGTGTTATTGAGGGATGATCAGCCTAGTAGTGTCAATTTCCATGTGGTTGCCATTGTGGGTATGCCGGGACTTGGAAAGACTACAATTGCAGGTCATGTCTTCAATGATGATGCAATGGAACGGTTTAGTCCCAAAGTATGGGTATCTGTGTCTGATAACTTCAATCTTGTGAGAGTGACAAAGGCAATTCTGGAATCAGTTATATCTGCACACTGTGATTTGGAGGAGTTCAGTCAAATTCAGGAGAGTCTGAGCAAGGCATTGGCAAGTAAAAAGTTTTTGGTTGTTTTAGATGATGTCTGGAACACATGTGACTATGATCTATGGGCAAAGCTGCAGTCTGCCTTTCGTGGCGGAGCATTGGGAAGTAAGGTAATTGTGACAACTCGTGACACACAAGTTGCTAAGATGATGAGATCCATTGAAGTTCATAACTTGGGGTGCATGTCAGATGATGATTGTTGGGAAGTATTTGGGCAACATGCATTCTTGAATGTTGAGAATGGGAGACTACAAAGCTTTGAGTTGTTTCGGGAGAAAGTTGTAGCAAAATGTGGTGGGTTGCCCTTGGCTGCAAGAGTCCTTGGTGGTCTTTTAGGCTGTAAAGAGATAGACGAATGGGAAGAAATATTGAACAGTAAATTATGGAGTCTATGTGATAACAGTGGCATTCTCCCAGTATTAAAATTGAGCTATCACTATCTCTCTTCAAATTTGAAAAGGTGCTTTGCCTATTGCTCAATACTTCCAAATGACTATGAATTTGGGGAGAGGCAGTTGATCCTTCTATGGATGGCAGAGGGTCTCGTTCAGCAGTCACATGAATGTAAACAACTGGAACATTTAGGGAGAACCTATTTTCGGGAGCTATTGTCCAGGTCATTGTTTCAAAGGTCTAGTAAAAACCATTCACAATTTGTTATGCATGACCTTGTTGGTGATTTGTCACGGTGGGCTGCAGGAGACATGTGTTTCAGATTGGAGGATAAGCTGGATGTTAGATGTTCTTCAAAGGTTCGCCATTCCTCTTACATTTCTGGTCAATTTGATGGTGTTCAAAAATTCAAAGAATTTTTTGAAGTCAGGCGTTTGCGGACTTTCTTACCACTTTCAGTTTCAGGTCGATCAAATTATCTAACTCGTAATGTTACTTTTGTTCTCTTACCAAAGTTGAAGTACTTGAGGTCACTCTCTTTTAATGGCTATCAAATAACTGAGTTACCCAATAGAATTGGTAAGCTGAAGCATCTGCGGTACCTTGATCTTTCTCACACAGAGATAACAACTTTGCCAGAATCCACCAGCAATCTGTACAACTTACAGACGTTGATATTAGAAAATTGTGCTCGTTTGAAGGCACTGCCTGCAGACATGAGGAATCTAACTGATCTGCGGCACCTTAACAATTCAGATGTACCTTCATTGGAAGAAATGCCTGTGAAACTAGGTAGATTGACTAATCTTCAAACACTGAGTACTTTTGTGCTGGGCAAAGGTAGCAAGTCAGGAATAAGTGAGATAGGTCCTTTATTGTGTCTCCAAGGGACATTGTGCCTCTCAAGATTGGAGAATGTGACTGATGTTGATGATGCAAGGAGGGCTGACTTGAATAGCAAGGAAGGGATTGATTCATTGCAACTAGAATGGAGAGAATCAGGAGAAAGGGAATCAGATGTTTTTGAAATGTTAAAACCTCATAGGAAGCTCAAAGTGCTCACTGTAAAAGGTTATGGAGGTCTTACATTTTCAACATGGACTGGACACCCTTCATTCTCTTATATGACGCATGTAAAGTTGGAACATTGTAAAAATTGTCGATTCTTGCCTCCACTTGGACAGTTGTCTTCTCTGAAAGAACTTTGTATAAAAGGAATGTCTACAGTGGAAAGCGTGGGTCTTGAGTTTTATGGAGAGAGTAGCCTGCCTTTTCCTGTATTGGAGACCCTGGAGTTCAAGGATATGCAGAAATGGAGGGAGTGGCTTCCTCGGGTGCAAGATCAAGGAATTGAAGTTTTCCCTTGCTTGAAAATGGTTTGTATCTCTGGATGCCCCATACTGGAGGGGAGATTACCTGAAAACCTTGATCTATTATCGAAGCTTGCCATTGATGGATGCGGGCAATTGGTGGTTTCAATTACCAGTTATAAACGTCTTCGTGATTTGAAAATTCATGTTTGCAAAATGCTGGTACATACAAGTGGGGTTGAATTTGAGTTACTAGAGGCGATGGAGCTTTCATGTATTTCAGAGTTTAGACTCCACATGGGCGGATTCAAGAGAGACTTAACAAAGCTTAAAAATCTGAGAATTACTAGTTGTGAGGAGCTGACATCTTTATGGAAAAATGAGGATAGATTGCTGCAACGTCCGATTTATAAAGGAAACAGCTCTGACTCTTACTTTGTTCAAGAGCTGAAGTCACTTCAATGTCTTCACATAAATGATTGCTCAAATCTAGTTTCTTTTTCAAAGGTTAGCCTGCCACCTTCTCTTAAAGAAATAAAGATTGAGTGGTGCAGTTCTCTTATAAGATACCAGATACCCCCAAACGTAAGAAGTCTATACATCGATTCCTGCATAAACTTGGAATCATTAGTGGGGGAGGACAAGGAAGGTGAGGATCCTTCATCTTCCTTTTCTCCTTGTTTGATACAAGAGGAGGCATCCTGCCTCGAGTACTTGAGCATAGTGAATTGTATGAGATTTACAACCTTATCATCCACAGGCCAGCTAGCCAGGGGACTTAAACATATTATCATAAATCATTGTCACCAGCTAGAATCAATAACCGATAGCTTCCATGTTAACACCTTCCTTGAAGAAATTGTGATTAGGAGTTGTGGAAATCTTAAATCGTTACCAGAGGGCTTATGTGACCTCACCAATCTTCAAGTCTTTAAGATTTGGGACTGTGAAAGTCTTGTTTCTTTCCCAAGAGGAGGGTTGCCAACAAGAACCTCCAGCTTGAAAGAGATCGAGGTTATGAATTGTGATAACTTAGAGGCCCTTCCTAAAGACATGCAGAACCTCCACTGTCTTCAGAGTTTGAGGATTTCATACTGTGAAGGTTTCGCGCCATTTCTAGACAAGGGTTTGCCACCCAACCTAACATTCCTTGAGATTCGAAATCTCAACAGTTGTAGGCCACTCCTGGAAGCTCAAGGATTGCACAGACTCACTTCTCTTAGAGCGTTGGATATCAGGGGAAATGATTCACAACTAGTGTCATTTCCACCTGACAAGGAGATGGTGCTCCCCAAATCTCTCATTCTACTTCATATTGAAGGGTTCCCGAATCTGAGGTACCTATCCAGCAAGGGCTTTCAGTTCCTCCCCTCTCTTGAATCTCTTCAAGTCATCAGCTGTCCAAAGCTAGCATCCATTCCTGAGGAGGGACTCCCTCTTTCACTTACACAACTTTATATCTTTGGTTGTCCACTGCTAGTAAAGAACTGCAGACCTGGAAAAGGACAATACTGGCGCTCCATAGCTCACATCCCTTTCATATGGATTAAGGATGATAAGGGAGCAGACATATTTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1356

Amino Acids

153.74

Weight (kDa)

6.68

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 97 5e-21 Rx N-terminal domain
NB-ARC PF00931 166 - 337 8.9e-37 NB-ARC domain
WHD_DRP PF23559 422 - 489 2.7e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 558 - 669 1.5e-19 Leucine-rich repeat region
LRR_8 PF13855 568 - 622 7.7e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 678 - 799 2.3e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2667, 2898, 3050
Acc36I ACCTGC 1 cut(s) 602
Acc65I GGTACC 2 cut(s) 1776, 3825
AccB1I GGYRCC 3 cut(s) 1776, 1917, 3825
AccB7I CCANNNNNTGG 1 cut(s) 660
AccBSI CCGCTC 1 cut(s) 146
AccI GTMKAC 2 cut(s) 99, 2399
AccII CGCG 3 cut(s) 85, 161, 3596
AclWI GGATC 4 cut(s) 933, 1297, 3137, 3150
AcsI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 2729
AcuI CTGAAG 4 cut(s) 17, 1784, 2957, 3545
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 9 cut(s) 318, 1700, 1778, 1835, 1938, 1999, 2712, 3200, 3827
AfeI AGCGCT 1 cut(s) 129
AfiI CCNNNNNNNGG 9 cut(s) 488, 593, 660, 1383, 2354, 2578, 3461, 3914, 3989
AflIII ACRYGT 2 cut(s) 830, 1488
AhlI ACTAGT 2 cut(s) 2834, 3742
AjnI CCWGG 5 cut(s) 1397, 2466, 3257, 3672, 3982
AjuI GAANNNNNNNTTGG 2 cut(s) 3617, 3649
Alw21I GWGCWC 3 cut(s) 1870, 2238, 3777
Alw26I GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
AlwI GGATC 4 cut(s) 933, 1297, 3137, 3150
AlwNI CAGNNNCTG 1 cut(s) 40
Ama87I CYCGRG 2 cut(s) 2508, 3194
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 5 cut(s) 452, 479, 3247, 3525, 3665
ApeKI GCWGC 6 cut(s) 125, 151, 856, 1094, 1478, 2881
ApoI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 2729
ArsI GACNNNNNNTTYG 6 cut(s) 845, 877, 1268, 1300, 2906, 2938
AscI GGCGCGCC 1 cut(s) 83
Asp700I GAANNNNTTC 2 cut(s) 3733, 3870
Asp718I GGTACC 2 cut(s) 1776, 3825
AspLEI GCGC 6 cut(s) 85, 87, 130, 276, 3598, 4005
AspS9I GGNCC 3 cut(s) 479, 2045, 3526
AsuC2I CCSGG 1 cut(s) 588
AsuHPI GGTGA 4 cut(s) 1472, 3149, 3284, 3394
AsuII TTCGAA 1 cut(s) 3646
AsuNHI GCTAGC 2 cut(s) 3253, 3895
AvaI CYCGRG 2 cut(s) 2508, 3194
AvaII GGWCC 1 cut(s) 2045
AxyI CCTNAGG 1 cut(s) 3909
BamHI GGATCC 1 cut(s) 3142
BanI GGYRCC 3 cut(s) 1776, 1917, 3825
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 6 cut(s) 7, 613, 2358, 2666, 2945, 3551
Bbv12I GWGCWC 3 cut(s) 1870, 2238, 3777
BbvI GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BccI CCATC 6 cut(s) 470, 1309, 1565, 2624, 2743, 3763
BciT130I CCWGG 5 cut(s) 1399, 2468, 3259, 3674, 3984
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 588
BcoDI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BcuI ACTAGT 2 cut(s) 2834, 3742
BfmI CTRYAG 6 cut(s) 152, 857, 1479, 1887, 2400, 3976
BfoI RGCGCY 2 cut(s) 131, 4006
BfuAI ACCTGC 1 cut(s) 602
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
BlsI GCNGC 9 cut(s) 127, 153, 273, 456, 858, 1096, 1480, 1917, 2883
BmcAI AGTACT 3 cut(s) 1700, 1999, 3200
Bme1390I CCNGG 6 cut(s) 588, 1399, 2468, 3259, 3674, 3984
Bme18I GGWCC 1 cut(s) 2045
BmeT110I CYCGRG 2 cut(s) 2508, 3194
BmgT120I GGNCC 3 cut(s) 479, 2045, 3526
BmiI GGNNCC 5 cut(s) 1778, 1919, 3144, 3812, 3827
BmrFI CCNGG 6 cut(s) 588, 1399, 2468, 3259, 3674, 3984
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 7 cut(s) 622, 1777, 2101, 2558, 2624, 3194, 3908
BmtI GCTAGC 2 cut(s) 3257, 3899
BmuI ACTGGG 1 cut(s) 1193
BoxI GACNNNNGTC 1 cut(s) 2362
BpiI GAAGAC 6 cut(s) 7, 613, 2358, 2666, 2945, 3551
BpmI CTGGAG 3 cut(s) 2489, 2600, 3466
Bpu14I TTCGAA 1 cut(s) 3646
BpuEI CTTGAG 7 cut(s) 95, 1723, 2065, 2441, 3223, 3659, 3666
BpuMI CCSGG 1 cut(s) 588
Bsa29I ATCGAT 1 cut(s) 3090
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 1331, 2456
BsaJI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BsaXI ACNNNNNCTCC 8 cut(s) 1162, 1192, 1283, 1313, 1379, 1409, 3989, 4019
Bsc4I CCNNNNNNNGG 9 cut(s) 488, 593, 660, 1383, 2354, 2578, 3461, 3914, 3989
Bse1I ACTGG 6 cut(s) 1199, 1362, 2284, 2583, 2661, 4004
Bse21I CCTNAGG 1 cut(s) 3909
Bse3DI GCAATG 2 cut(s) 642, 2153
BseBI CCWGG 5 cut(s) 1399, 2468, 3259, 3674, 3984
BseCI ATCGAT 1 cut(s) 3090
BseDI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BseLI CCNNNNNNNGG 9 cut(s) 488, 593, 660, 1383, 2354, 2578, 3461, 3914, 3989
BseMI GCAATG 2 cut(s) 642, 2153
BseMII CTCAG 6 cut(s) 764, 1728, 1985, 2816, 3812, 3900
BseNI ACTGG 6 cut(s) 1199, 1362, 2284, 2583, 2661, 4004
BsePI GCGCGC 1 cut(s) 83
BseRI GAGGAG 6 cut(s) 48, 761, 2858, 3194, 3474, 3926
BseXI GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BseYI CCCAGC 1 cut(s) 2008
BsgI GTGCAG 3 cut(s) 144, 714, 3058
Bsh1236I CGCG 3 cut(s) 85, 161, 3596
BshFI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BshNI GGYRCC 3 cut(s) 1776, 1917, 3825
BshVI ATCGAT 1 cut(s) 3090
BsiHKAI GWGCWC 3 cut(s) 1870, 2238, 3777
BsiHKCI CYCGRG 2 cut(s) 2508, 3194
BsiSI CCGG 1 cut(s) 587
BslFI GGGAC 6 cut(s) 603, 636, 2080, 3276, 3443, 3930
BslI CCNNNNNNNGG 9 cut(s) 488, 593, 660, 1383, 2354, 2578, 3461, 3914, 3989
BsmAI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BsmFI GGGAC 6 cut(s) 603, 636, 2080, 3276, 3443, 3930
BsmI GAATGC 3 cut(s) 26, 1007, 1191
BsnI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
Bso31I GGTCTC 2 cut(s) 1331, 2456
BsoBI CYCGRG 2 cut(s) 2508, 3194
Bsp119I TTCGAA 1 cut(s) 3646
Bsp1286I GDGCHC 3 cut(s) 1870, 2238, 3777
Bsp1407I TGTACA 1 cut(s) 1833
BspANI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BspCNI CTCAG 6 cut(s) 765, 1729, 1986, 2817, 3813, 3901
BspDI ATCGAT 1 cut(s) 3090
BspFNI CGCG 3 cut(s) 85, 161, 3596
BspLI GGNNCC 5 cut(s) 1778, 1919, 3144, 3812, 3827
BspMAI CTGCAG 5 cut(s) 156, 861, 1483, 1891, 3980
BspMI ACCTGC 1 cut(s) 602
BspOI GCTAGC 2 cut(s) 3257, 3899
BspPI GGATC 4 cut(s) 933, 1297, 3137, 3150
BspT104I TTCGAA 1 cut(s) 3646
BspT107I GGYRCC 3 cut(s) 1776, 1917, 3825
BspTNI GGTCTC 2 cut(s) 1331, 2456
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 2 cut(s) 642, 2153
BsrGI TGTACA 1 cut(s) 1833
BsrI ACTGG 6 cut(s) 1199, 1362, 2284, 2583, 2661, 4004
BssECI CCNNGG 7 cut(s) 19, 1089, 1105, 2062, 2466, 2507, 3258
BssHII GCGCGC 1 cut(s) 83
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 3 cut(s) 1089, 1105, 2062
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 5 cut(s) 1399, 2468, 3259, 3674, 3984
Bst6I CTCTTC 2 cut(s) 1234, 3876
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 1886
BstAUI TGTACA 1 cut(s) 1833
BstBI TTCGAA 1 cut(s) 3646
BstENI CCTNNNNNAGG 1 cut(s) 3987
BstFNI CGCG 3 cut(s) 85, 161, 3596
BstH2I RGCGCY 2 cut(s) 131, 4006
BstHHI GCGC 6 cut(s) 85, 87, 130, 276, 3598, 4005
BstMAI GTCTC 6 cut(s) 1024, 1331, 1479, 2063, 2456, 2796
BstMWI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 454, 1886, 2019, 2120, 2233
BstNI CCWGG 5 cut(s) 1399, 2468, 3259, 3674, 3984
BstNSI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
BstPAI GACNNNNGTC 1 cut(s) 2362
BstSCI CCNGG 6 cut(s) 586, 1397, 2466, 3257, 3672, 3982
BstSFI CTRYAG 6 cut(s) 152, 857, 1479, 1887, 2400, 3976
BstUI CGCG 3 cut(s) 85, 161, 3596
BstV1I GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
BstV2I GAAGAC 6 cut(s) 7, 613, 2358, 2666, 2945, 3551
BstX2I RGATCY 2 cut(s) 938, 3142
BstYI RGATCY 2 cut(s) 938, 3142
Bsu15I ATCGAT 1 cut(s) 3090
Bsu36I CCTNAGG 1 cut(s) 3909
BsuRI GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
BsuTUI ATCGAT 1 cut(s) 3090
BtgZI GCGATG 1 cut(s) 2762
BtsI GCAGTG 2 cut(s) 1880, 3959
BtsIMutI CAGTG 8 cut(s) 733, 1192, 1880, 1991, 2238, 2409, 3553, 3959
BveI ACCTGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 6 cut(s) 85, 87, 130, 276, 3598, 4005
Cfr13I GGNCC 3 cut(s) 479, 2045, 3526
ClaI ATCGAT 1 cut(s) 3090
CseI GACGC 3 cut(s) 85, 167, 2314
Csp6I GTAC 9 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199, 3826
CspCI CAANNNNNGTGG 2 cut(s) 281, 316
CviQI GTAC 9 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199, 3826
DraIII CACNNNGTG 1 cut(s) 737
Eam1104I CTCTTC 2 cut(s) 1234, 3876
EarI CTCTTC 2 cut(s) 1234, 3876
EciI GGCGGA 2 cut(s) 891, 2805
Eco130I CCWWGG 3 cut(s) 1089, 1105, 2062
Eco31I GGTCTC 2 cut(s) 1331, 2456
Eco32I GATATC 2 cut(s) 387, 3721
Eco47I GGWCC 1 cut(s) 2045
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 4 cut(s) 17, 1784, 2957, 3545
Eco81I CCTNAGG 1 cut(s) 3909
Eco88I CYCGRG 2 cut(s) 2508, 3194
EcoNI CCTNNNNNAGG 1 cut(s) 3987
EcoO109I RGGNCCY 2 cut(s) 2045, 3526
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 5 cut(s) 1397, 2466, 3257, 3672, 3982
EcoRV GATATC 2 cut(s) 387, 3721
EcoT14I CCWWGG 3 cut(s) 1089, 1105, 2062
EcoT22I ATGCAT 2 cut(s) 1009, 1449
ErhI CCWWGG 3 cut(s) 1089, 1105, 2062
FalI AAGNNNNNCTT 2 cut(s) 2789, 2821
FaqI GGGAC 6 cut(s) 603, 636, 2080, 3276, 3443, 3930
FauI CCCGC 1 cut(s) 2630
FauNDI CATATG 1 cut(s) 4028
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 2 cut(s) 99, 2399
Fnu4HI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
Fsp4HI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
GlaI GCGC 6 cut(s) 84, 86, 129, 275, 3597, 4004
GluI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
GsaI CCCAGC 1 cut(s) 2012
GsuI CTGGAG 3 cut(s) 2489, 2600, 3466
HaeII RGCGCY 2 cut(s) 131, 4006
HaeIII GGCC 5 cut(s) 454, 481, 3249, 3527, 3667
HapII CCGG 1 cut(s) 587
HgaI GACGC 3 cut(s) 85, 167, 2314
HhaI GCGC 6 cut(s) 85, 87, 130, 276, 3598, 4005
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 6 cut(s) 83, 85, 128, 274, 3596, 4003
HinP1I GCGC 6 cut(s) 83, 85, 128, 274, 3596, 4003
HincII GTYRAC 3 cut(s) 54, 100, 3328
HindII GTYRAC 3 cut(s) 54, 100, 3328
HindIII AAGCTT 3 cut(s) 1039, 2618, 2813
HpaI GTTAAC 2 cut(s) 54, 3328
HpaII CCGG 1 cut(s) 587
HphI GGTGA 4 cut(s) 1472, 3149, 3284, 3394
Hpy166II GTNNAC 6 cut(s) 54, 100, 1352, 2400, 3328, 3959
Hpy8I GTNNAC 6 cut(s) 54, 100, 1352, 2400, 3328, 3959
Hpy99I CGWCG 1 cut(s) 101
HpyCH4IV ACGT 5 cut(s) 279, 1847, 2671, 2887, 3072
HpyF10VI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 454, 1886, 2019, 2120, 2233
HpySE526I ACGT 5 cut(s) 279, 1847, 2671, 2887, 3072
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 6 cut(s) 83, 85, 128, 274, 3596, 4003
KpnI GGTACC 2 cut(s) 1780, 3829
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 2 cut(s) 54, 3328
LmnI GCTCC 9 cut(s) 143, 323, 878, 1387, 2752, 2845, 3780, 4010, 4049
Lsp1109I GCAGC 6 cut(s) 137, 138, 843, 1081, 1465, 2868
LweI GCATC 7 cut(s) 622, 1777, 2101, 2558, 2624, 3194, 3908
MaeII ACGT 5 cut(s) 279, 1847, 2671, 2887, 3072
MbiI CCGCTC 1 cut(s) 146
MfeI CAATTG 2 cut(s) 606, 2642
MflI RGATCY 2 cut(s) 938, 3142
MhlI GDGCHC 3 cut(s) 1870, 2238, 3777
MlyI GAGTC 7 cut(s) 778, 1110, 1180, 2772, 2909, 3690, 3912
MmeI TCCRAC 2 cut(s) 2298, 3696
Mph1103I ATGCAT 2 cut(s) 1009, 1449
MroXI GAANNNNTTC 2 cut(s) 3733, 3870
MslI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
MspA1I CMGCKG 1 cut(s) 3885
MspI CCGG 1 cut(s) 587
MspR9I CCNGG 6 cut(s) 588, 1399, 2468, 3259, 3674, 3984
MunI CAATTG 2 cut(s) 606, 2642
Mva1269I GAATGC 3 cut(s) 26, 1007, 1191
MvaI CCWGG 5 cut(s) 1399, 2468, 3259, 3674, 3984
MvnI CGCG 3 cut(s) 85, 161, 3596
MwoI GCNNNNNNNGC 9 cut(s) 93, 134, 143, 271, 454, 1886, 2019, 2120, 2233
NciI CCSGG 1 cut(s) 588
NdeI CATATG 1 cut(s) 4028
NheI GCTAGC 2 cut(s) 3253, 3895
NlaIV GGNNCC 5 cut(s) 1778, 1919, 3144, 3812, 3827
NmeAIII GCCGAG 1 cut(s) 483
NsiI ATGCAT 2 cut(s) 1009, 1449
NspI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
NspV TTCGAA 1 cut(s) 3646
PaeR7I CTCGAG 1 cut(s) 3194
PalAI GGCGCGCC 1 cut(s) 83
PauI GCGCGC 1 cut(s) 83
PciI ACATGT 2 cut(s) 830, 1488
PctI GAATGC 3 cut(s) 26, 1007, 1191
PdmI GAANNNNTTC 2 cut(s) 3733, 3870
PflMI CCANNNNNTGG 1 cut(s) 660
PfoI TCCNGGA 1 cut(s) 3672
PkrI GCNGC 9 cut(s) 127, 153, 273, 456, 858, 1096, 1480, 1917, 2883
PleI GAGTC 7 cut(s) 777, 1109, 1179, 2772, 2909, 3690, 3912
PpsI GAGTC 7 cut(s) 777, 1109, 1179, 2772, 2909, 3690, 3912
PpuMI RGGWCCY 1 cut(s) 2045
PscI ACATGT 2 cut(s) 830, 1488
PshAI GACNNNNGTC 1 cut(s) 2362
PsiI TTATAA 3 cut(s) 2667, 2898, 3050
Psp5II RGGWCCY 1 cut(s) 2045
Psp6I CCWGG 5 cut(s) 1397, 2466, 3257, 3672, 3982
PspFI CCCAGC 1 cut(s) 2008
PspGI CCWGG 5 cut(s) 1397, 2466, 3257, 3672, 3982
PspN4I GGNNCC 5 cut(s) 1778, 1919, 3144, 3812, 3827
PspPI GGNCC 3 cut(s) 479, 2045, 3526
PspPPI RGGWCCY 1 cut(s) 2045
PspXI VCTCGAGB 1 cut(s) 3194
PstI CTGCAG 5 cut(s) 156, 861, 1483, 1891, 3980
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 2 cut(s) 938, 3142
PteI GCGCGC 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 3885
RsaI GTAC 9 cut(s) 318, 1700, 1778, 1835, 1938, 1999, 2712, 3200, 3827
RsaNI GTAC 9 cut(s) 317, 1699, 1777, 1834, 1937, 1998, 2711, 3199, 3826
RseI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
SalI GTCGAC 1 cut(s) 98
SatI GCNGC 9 cut(s) 126, 152, 272, 455, 857, 1095, 1479, 1916, 2882
Sau96I GGNCC 3 cut(s) 479, 2045, 3526
ScaI AGTACT 3 cut(s) 1700, 1999, 3200
SchI GAGTC 7 cut(s) 778, 1110, 1180, 2772, 2909, 3690, 3912
ScrFI CCNGG 6 cut(s) 588, 1399, 2468, 3259, 3674, 3984
SduI GDGCHC 3 cut(s) 1870, 2238, 3777
SfaNI GCATC 7 cut(s) 622, 1777, 2101, 2558, 2624, 3194, 3908
SfcI CTRYAG 6 cut(s) 152, 857, 1479, 1887, 2400, 3976
Sfr274I CTCGAG 1 cut(s) 3194
SfuI TTCGAA 1 cut(s) 3646
SgsI GGCGCGCC 1 cut(s) 83
SinI GGWCC 1 cut(s) 2045
SlaI CTCGAG 1 cut(s) 3194
SmiMI CAYNNNNRTG 5 cut(s) 1345, 2718, 2857, 2947, 2961
SmlI CTYRAG 8 cut(s) 110, 1702, 2080, 2420, 3194, 3202, 3638, 3681
SmoI CTYRAG 8 cut(s) 110, 1702, 2080, 2420, 3194, 3202, 3638, 3681
SpeI ACTAGT 2 cut(s) 2834, 3742
SspI AATATT 1 cut(s) 1152
StyD4I CCNGG 6 cut(s) 586, 1397, 2466, 3257, 3672, 3982
StyI CCWWGG 3 cut(s) 1089, 1105, 2062
TaiI ACGT 5 cut(s) 282, 1850, 2674, 2890, 3075
TaqI TCGA 9 cut(s) 45, 99, 1645, 2338, 2615, 3090, 3195, 3498, 3646
TatI WGTACW 4 cut(s) 1698, 1833, 1997, 3198
TauI GCSGC 3 cut(s) 274, 457, 1918
TscAI CASTG 8 cut(s) 740, 1192, 1887, 1998, 2245, 2409, 3560, 3966
TseI GCWGC 6 cut(s) 125, 151, 856, 1094, 1478, 2881
TspRI CASTG 8 cut(s) 740, 1192, 1887, 1998, 2245, 2409, 3560, 3966
Van91I CCANNNNNTGG 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 2045
XagI CCTNNNNNAGG 1 cut(s) 3987
XapI RAATTY 8 cut(s) 346, 759, 1234, 1283, 1583, 1592, 2688, 2729
XbaI TCTAGA 1 cut(s) 3604
XceI RCATGY 7 cut(s) 299, 834, 970, 1007, 1492, 2312, 3544
XhoI CTCGAG 1 cut(s) 3194
XmiI GTMKAC 2 cut(s) 99, 2399
XmnI GAANNNNTTC 2 cut(s) 3733, 3870
ZrmI AGTACT 3 cut(s) 1700, 1999, 3200
Zsp2I ATGCAT 2 cut(s) 1009, 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.