RLG00000024011

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000466
Physical Location & Seq
Reverse (-)
30873206 .. 30873736
531 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024011

Sequence Viewer

Length: 531 bp
ATGTCGGAGGATAATCAAGAACCGGTTGATGTTGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTCGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGCAGACACTCGCAAGGGTATTACATTTGAATTATACGATCGACTGAAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTGGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGGTCCTACTGATGTACGATATCAGAAGAGGAGTTTCGGGGATGCTTTTACTAAGCATGAAAAATCAGAGCAACAAAGATCAGAGAAGGTGCCGCGGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCACACACAGAATTATAGGTAA

Protein Analysis

177

Amino Acids

20.46

Weight (kDa)

6.84

Isoelectric Point (pI)

64.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 175 3.5e-48 TIR domain
TIR_2 PF13676 29 - 142 3.4e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 460
AccII CGCG 1 cut(s) 466
AciI CCGC 2 cut(s) 464, 466
AcsI RAATTY 2 cut(s) 348, 496
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 1 cut(s) 387
AgeI ACCGGT 1 cut(s) 22
AgsI TTSAA 2 cut(s) 134, 197
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
ApoI RAATTY 2 cut(s) 348, 496
AsiGI ACCGGT 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 374
AvaII GGWCC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 342
BccI CCATC 1 cut(s) 501
BceAI ACGGC 1 cut(s) 246
BisI GCNGC 1 cut(s) 464
BlsI GCNGC 1 cut(s) 465
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 375, 462
BmsI GCATC 4 cut(s) 25, 53, 403, 466
BpiI GAAGAC 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 68, 464
BsaWI WCCGGW 1 cut(s) 22
Bse118I RCCGGY 1 cut(s) 22
BseDI CCNNGG 2 cut(s) 68, 464
BseGI GGATG 4 cut(s) 187, 211, 418, 512
BseRI GAGGAG 2 cut(s) 57, 415
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 145
BshNI GGYRCC 1 cut(s) 460
BshTI ACCGGT 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 145
BsiSI CCGG 1 cut(s) 23
BsmI GAATGC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 142, 449
BspACI CCGC 2 cut(s) 464, 466
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 375, 462
BspT107I GGYRCC 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 2 cut(s) 68, 464
BssMI GATC 2 cut(s) 142, 449
Bst6I CTCTTC 3 cut(s) 54, 289, 392
BstDEI CTNAG 2 cut(s) 308, 423
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 4 cut(s) 187, 211, 418, 512
BstFNI CGCG 1 cut(s) 466
BstKTI GATC 2 cut(s) 145, 452
BstMBI GATC 2 cut(s) 142, 449
BstMCI CGRYCG 1 cut(s) 145
BstUI CGCG 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 342
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 4 cut(s) 187, 211, 418, 512
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 374
Cfr42I CCGCGG 1 cut(s) 467
Csp6I GTAC 1 cut(s) 386
CspAI ACCGGT 1 cut(s) 22
CviAII CATG 4 cut(s) 80, 178, 328, 428
CviJI RGCY 1 cut(s) 65
CviKI_1 RGCY 1 cut(s) 65
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 2 cut(s) 308, 423
DpnI GATC 2 cut(s) 144, 451
DpnII GATC 2 cut(s) 142, 449
DraI TTTAAA 1 cut(s) 483
DraIII CACNNNGTG 1 cut(s) 294
Eam1104I CTCTTC 3 cut(s) 54, 289, 392
EarI CTCTTC 3 cut(s) 54, 289, 392
Eco32I GATATC 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 348
EcoRV GATATC 1 cut(s) 392
EcoT22I ATGCAT 1 cut(s) 329
FaeI CATG 4 cut(s) 83, 181, 331, 431
FaiI YATR 8 cut(s) 81, 139, 179, 279, 329, 369, 429, 525
FatI CATG 4 cut(s) 79, 177, 327, 427
Fnu4HI GCNGC 1 cut(s) 464
FokI GGATG 4 cut(s) 194, 218, 425, 519
Fsp4HI GCNGC 1 cut(s) 464
GluI GCNGC 1 cut(s) 464
HapII CCGG 1 cut(s) 23
Hin1II CATG 4 cut(s) 83, 181, 331, 431
HinfI GANTC 2 cut(s) 165, 242
HpaII CCGG 1 cut(s) 23
Hpy188I TCNGA 4 cut(s) 7, 396, 439, 454
Hpy188III TCNNGA 2 cut(s) 17, 504
HpyAV CCTTC 3 cut(s) 63, 203, 451
HpyCH4V TGCA 3 cut(s) 44, 107, 327
HpyF3I CTNAG 2 cut(s) 308, 423
Hsp92II CATG 4 cut(s) 83, 181, 331, 431
KspI CCGCGG 1 cut(s) 467
Kzo9I GATC 2 cut(s) 142, 449
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 4 cut(s) 36, 85, 142, 489
LweI GCATC 4 cut(s) 25, 53, 403, 466
MalI GATC 2 cut(s) 144, 451
MboI GATC 2 cut(s) 142, 449
MboII GAAGA 5 cut(s) 41, 276, 344, 347, 409
MluCI AATT 6 cut(s) 134, 234, 255, 348, 496, 520
MnlI CCTC 6 cut(s) 49, 78, 155, 292, 295, 393
Mph1103I ATGCAT 1 cut(s) 329
MseI TTAA 2 cut(s) 237, 482
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 1 cut(s) 23
Mva1269I GAATGC 1 cut(s) 329
MvnI CGCG 1 cut(s) 466
NdeII GATC 2 cut(s) 142, 449
NlaIII CATG 4 cut(s) 83, 181, 331, 431
NlaIV GGNNCC 2 cut(s) 375, 462
NsiI ATGCAT 1 cut(s) 329
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 165, 242
PinAI ACCGGT 1 cut(s) 22
PkrI GCNGC 1 cut(s) 465
Ple19I CGATCG 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 375, 462
PspPI GGNCC 1 cut(s) 374
PspPPI RGGWCCY 1 cut(s) 374
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SacII CCGCGG 1 cut(s) 467
SaqAI TTAA 2 cut(s) 237, 482
SatI GCNGC 1 cut(s) 464
Sau3AI GATC 2 cut(s) 142, 449
Sau96I GGNCC 1 cut(s) 374
SetI ASST 5 cut(s) 67, 195, 251, 462, 530
SfaNI GCATC 4 cut(s) 25, 53, 403, 466
Sfr303I CCGCGG 1 cut(s) 467
SgrBI CCGCGG 1 cut(s) 467
SinI GGWCC 1 cut(s) 374
Sse9I AATT 6 cut(s) 134, 234, 255, 348, 496, 520
SsiI CCGC 2 cut(s) 464, 466
TaqI TCGA 1 cut(s) 145
TasI AATT 6 cut(s) 134, 234, 255, 348, 496, 520
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 2 cut(s) 165, 242
Tru1I TTAA 2 cut(s) 237, 482
Tru9I TTAA 2 cut(s) 237, 482
TspDTI ATGAA 3 cut(s) 45, 166, 444
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 2 cut(s) 348, 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.