Rroxscaffold_1G00003030

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000466
Physical Location & Seq
Forward (+)
3520936 .. 3522952
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003030.1

Sequence Viewer

Length: 837 bp
ATGCCTGAGCCGATATCGAATGATAATCGAGAATCGGTCGTTGTGGATCCCTCTGCACCATCATCAGCTCCCATTGTGGAAAAGCCTGAGCCAATAATATTGGAGGATAATCAAGAATCGGTGGACCTCAATCCCTATGCGTCTCTTCTTTCACCAGCTCCTCCGCAGAAGTATGATGTTTTTCCGAATTTCAGGGGTGCAGACACTCGTAAGGATATTCTATCCCACTTATACCATGAATTGCAAACTGTGAGTGGGATTCAAACATTTAAGGATGACAGGGAGTTTGAAATAGGGGACCCTATTTTTGCAACTCTGCTAAAGGCAATTGGAGAATCAAAATTTGCAATTGTTGTTTTGTCGAAAAATTATGCTGAATCTCCCTGGTGTTTGGAGGAACTAACAAAGATTTGTGAATGCATGATACACAATACCAGAATTCTACCTCTTTTTTATCATGTCGAACCTACTGATGTACGATTTCAGAAGGGTTGTTTTAAAGATGCTTTCAGTAAGCATGAAAACTCGGTGCGGCACAATTCAGAGAAGGTGCAGCAATGGAGAAAAGCTTTAAAAGAAGTGGCCGATTTCTCTGGGTGGAATACAGAGGATTTAAATCATTATCCAGTCTTTACAAGATCTCCTAGTTCCTCACTGCATTCCAGTTTGTCCTCTGCCAACCAGTTTTCCAAAAATTCTTCTTGTTCTGGTTCACAACAAGACTCGACAATTCCAATGACTCAAAAGGCATTGAGCTTCCCTCTCGTTAAGATCGAGTGTCCCATAAGTACTGCTACAAAGGGGGTGCTTTGCCCAGCTTGGACAGGAACACTCTAA

Protein Analysis

278

Amino Acids

31.09

Weight (kDa)

5.52

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 57 - 214 1.7e-46 TIR domain
TIR_2 PF13676 60 - 156 6.9e-13 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 164, 532
AclWI GGATC 2 cut(s) 41, 54
AcoI YGGCCR 1 cut(s) 582
AcsI RAATTY 4 cut(s) 187, 341, 438, 694
AfaI GTAC 2 cut(s) 477, 790
AgsI TTSAA 2 cut(s) 263, 290
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 5 cut(s) 68, 158, 569, 756, 818
AluI AGCT 5 cut(s) 68, 158, 569, 756, 818
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 2 cut(s) 41, 54
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 1 cut(s) 553
ApoI RAATTY 4 cut(s) 187, 341, 438, 694
ArsI GACNNNNNNTTYG 4 cut(s) 269, 290, 301, 322
AspS9I GGNCC 2 cut(s) 124, 298
AsuHPI GGTGA 1 cut(s) 144
AvaII GGWCC 2 cut(s) 124, 298
BamHI GGATCC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 565
BccI CCATC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 645
BglII AGATCT 1 cut(s) 638
BisI GCNGC 2 cut(s) 533, 554
BlsI GCNGC 2 cut(s) 534, 555
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 2 cut(s) 124, 298
BmgT120I GGNCC 2 cut(s) 124, 298
BmiI GGNNCC 3 cut(s) 48, 299, 300
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 1 cut(s) 493
BplI GAGNNNNNCTC 2 cut(s) 745, 777
Bpu10I CCTNAGC 2 cut(s) 6, 87
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bse1I ACTGG 3 cut(s) 626, 663, 682
Bse3DI GCAATG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 383
BseGI GGATG 1 cut(s) 280
BseJI GATNNNNATC 1 cut(s) 615
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 3 cut(s) 626, 663, 682
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 814
BsgI GTGCAG 3 cut(s) 39, 219, 572
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 584
BsiEI CGRYCG 1 cut(s) 39
BslFI GGGAC 2 cut(s) 311, 765
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 311, 765
BsmI GAATGC 2 cut(s) 422, 658
BsnI GGCC 1 cut(s) 584
Bsp143I GATC 3 cut(s) 46, 638, 771
BspACI CCGC 2 cut(s) 164, 532
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 48, 299, 300
BspPI GGATC 2 cut(s) 41, 54
BsrDI GCAATG 1 cut(s) 563
BsrI ACTGG 3 cut(s) 626, 663, 682
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 3 cut(s) 46, 638, 771
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 6, 87
BstF5I GGATG 1 cut(s) 280
BstKTI GATC 3 cut(s) 49, 641, 774
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 3 cut(s) 46, 638, 771
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 46, 638
BstYI RGATCY 2 cut(s) 46, 638
BsuRI GGCC 1 cut(s) 584
BtsCI GGATG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 653
BtsIMutI CAGTG 1 cut(s) 653
Cfr13I GGNCC 2 cut(s) 124, 298
CseI GACGC 1 cut(s) 129
Csp6I GTAC 2 cut(s) 476, 789
CviAII CATG 4 cut(s) 236, 421, 458, 518
CviJI RGCY 9 cut(s) 10, 68, 85, 91, 158, 569, 584, 756, 818
CviKI_1 RGCY 9 cut(s) 10, 68, 85, 91, 158, 569, 584, 756, 818
CviQI GTAC 2 cut(s) 476, 789
DdeI CTNAG 2 cut(s) 6, 87
DpnI GATC 3 cut(s) 48, 640, 773
DpnII GATC 3 cut(s) 46, 638, 771
DraI TTTAAA 3 cut(s) 499, 573, 615
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 124, 298
EcoO109I RGGNCCY 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 383
EcoRV GATATC 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 422
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 4 cut(s) 239, 424, 461, 521
FaiI YATR 9 cut(s) 138, 174, 232, 237, 372, 422, 459, 519, 785
FaqI GGGAC 2 cut(s) 311, 765
FatI CATG 4 cut(s) 235, 420, 457, 517
Fnu4HI GCNGC 2 cut(s) 533, 554
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 2 cut(s) 533, 554
FspBI CTAG 1 cut(s) 645
GluI GCNGC 2 cut(s) 533, 554
GsaI CCCAGC 1 cut(s) 818
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 239, 424, 461, 521
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 7 cut(s) 32, 116, 259, 335, 377, 722, 739
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 124, 713
Hpy188I TCNGA 3 cut(s) 186, 486, 544
Hpy188III TCNNGA 2 cut(s) 29, 113
Hpy8I GTNNAC 2 cut(s) 124, 713
HpyAV CCTTC 2 cut(s) 481, 541
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 8 cut(s) 56, 200, 244, 311, 347, 420, 553, 658
HpyF3I CTNAG 2 cut(s) 6, 87
Hsp92II CATG 4 cut(s) 239, 424, 461, 521
KflI GGGWCCC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 46, 638, 771
LmnI GCTCC 2 cut(s) 73, 163
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 645
MalI GATC 3 cut(s) 48, 640, 773
MboI GATC 3 cut(s) 46, 638, 771
MboII GAAGA 2 cut(s) 137, 690
MfeI CAATTG 2 cut(s) 327, 348
MflI RGATCY 2 cut(s) 46, 638
MlyI GAGTC 2 cut(s) 716, 733
Mph1103I ATGCAT 1 cut(s) 422
MseI TTAA 5 cut(s) 270, 498, 572, 614, 768
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 2 cut(s) 327, 348
Mva1269I GAATGC 2 cut(s) 422, 658
MvaI CCWGG 1 cut(s) 385
NdeII GATC 3 cut(s) 46, 638, 771
NlaIII CATG 4 cut(s) 239, 424, 461, 521
NlaIV GGNNCC 3 cut(s) 48, 299, 300
NsiI ATGCAT 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PctI GAATGC 2 cut(s) 422, 658
PfeI GAWTC 5 cut(s) 32, 116, 259, 335, 377
PkrI GCNGC 2 cut(s) 534, 555
PleI GAGTC 2 cut(s) 716, 733
PpsI GAGTC 2 cut(s) 716, 733
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 383
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 48, 299, 300
PspPI GGNCC 2 cut(s) 124, 298
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 46, 638
RsaI GTAC 2 cut(s) 477, 790
RsaNI GTAC 2 cut(s) 476, 789
SaqAI TTAA 5 cut(s) 270, 498, 572, 614, 768
SatI GCNGC 2 cut(s) 533, 554
Sau3AI GATC 3 cut(s) 46, 638, 771
Sau96I GGNCC 2 cut(s) 124, 298
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 2 cut(s) 716, 733
ScrFI CCNGG 1 cut(s) 385
SetI ASST 9 cut(s) 70, 129, 160, 448, 469, 552, 571, 758, 820
SfaNI GCATC 1 cut(s) 493
SinI GGWCC 2 cut(s) 124, 298
SmiI ATTTAAAT 1 cut(s) 615
SsiI CCGC 2 cut(s) 164, 532
SspI AATATT 1 cut(s) 99
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 1 cut(s) 383
SwaI ATTTAAAT 1 cut(s) 615
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 6 cut(s) 17, 28, 362, 462, 725, 774
TaqII GACCGA 1 cut(s) 25
TatI WGTACW 1 cut(s) 788
TauI GCSGC 1 cut(s) 535
TfiI GAWTC 5 cut(s) 32, 116, 259, 335, 377
Tru1I TTAA 5 cut(s) 270, 498, 572, 614, 768
Tru9I TTAA 5 cut(s) 270, 498, 572, 614, 768
TscAI CASTG 1 cut(s) 660
TseI GCWGC 1 cut(s) 553
TspDTI ATGAA 2 cut(s) 252, 534
TspRI CASTG 1 cut(s) 660
VpaK11BI GGWCC 2 cut(s) 124, 298
XapI RAATTY 4 cut(s) 187, 341, 438, 694
XspI CTAG 1 cut(s) 645
ZrmI AGTACT 1 cut(s) 790
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.