Rroxscaffold_4G00326470

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000466
Physical Location & Seq
Reverse (-)
58581258 .. 58592191
10934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326470.1

Sequence Viewer

Length: 393 bp
ATGCTTATTCGTCATGGTGCTTGGATGAACTCGTCAAGAACTCAAGATTATTCAATGCATGAAAGGGATGGGCCAACAAGTCCTCCCGTCTTCTACTGGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGATGGTTTGAGCTCAAAAGGAAACCCCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGACAAGAATGACGGACTAAATTCCTGGGGAGCTGCTTTGACTGTGGCCAATCTTTCTGGCTGGAATTTTACGCCTCGACAAGGATTGTTTGGTGGAAGTGAGTCCTTTTTGGTTGTTGAAAGTGCTCGATTGAAAGTAATAAGAACTACTCATACTCCTAATGTAAGAACAGCGGTTATAGACACCATGAGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

130

Amino Acids

14.94

Weight (kDa)

6.98

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 368
AclWI GGATC 2 cut(s) 98, 111
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 2 cut(s) 214, 259
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 3 cut(s) 54, 314, 328
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 2 cut(s) 142, 227
AluI AGCT 2 cut(s) 142, 227
Alw21I GWGCWC 2 cut(s) 144, 322
AlwI GGATC 2 cut(s) 98, 111
AoxI GGCC 2 cut(s) 71, 240
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 2 cut(s) 214, 259
AspS9I GGNCC 1 cut(s) 71
BalI TGGCCA 1 cut(s) 242
BamHI GGATCC 1 cut(s) 103
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 2 cut(s) 144, 322
BbvI GCAGC 1 cut(s) 214
BccI CCATC 2 cut(s) 62, 126
BciT130I CCWGG 1 cut(s) 220
BisI GCNGC 2 cut(s) 119, 228
BlsI GCNGC 2 cut(s) 120, 229
Bme1390I CCNGG 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 105, 122
BmrFI CCNGG 1 cut(s) 220
BmrI ACTGGG 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 334, 364
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 3 cut(s) 30, 73, 137
BseLI CCNNNNNNNGG 1 cut(s) 275
BseNI ACTGG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 214
BshFI GGCC 2 cut(s) 73, 242
BshNI GGYRCC 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 144, 322
BslI CCNNNNNNNGG 1 cut(s) 275
BsnI GGCC 2 cut(s) 73, 242
Bsp1286I GDGCHC 2 cut(s) 144, 322
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 2 cut(s) 118, 368
BspANI GGCC 2 cut(s) 73, 242
BspLI GGNNCC 2 cut(s) 105, 122
BspPI GGATC 2 cut(s) 98, 111
BspT107I GGYRCC 1 cut(s) 120
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 238
BstENI CCTNNNNNAGG 1 cut(s) 273
BstF5I GGATG 3 cut(s) 30, 73, 137
BstKTI GATC 1 cut(s) 106
BstMBI GATC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 2 cut(s) 73, 242
BtsCI GGATG 3 cut(s) 30, 73, 137
Cfr13I GGNCC 1 cut(s) 71
CviAII CATG 4 cut(s) 14, 59, 182, 382
CviJI RGCY 6 cut(s) 73, 142, 227, 242, 255, 387
CviKI_1 RGCY 6 cut(s) 73, 142, 227, 242, 255, 387
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 240
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 218
EcoT22I ATGCAT 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 4 cut(s) 17, 62, 185, 385
FaiI YATR 7 cut(s) 15, 60, 183, 195, 348, 374, 383
FatI CATG 4 cut(s) 13, 58, 181, 381
Fnu4HI GCNGC 2 cut(s) 119, 228
FokI GGATG 3 cut(s) 37, 80, 144
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 2 cut(s) 119, 228
GluI GCNGC 2 cut(s) 119, 228
HaeIII GGCC 2 cut(s) 73, 242
Hin1II CATG 4 cut(s) 17, 62, 185, 385
HinfI GANTC 1 cut(s) 296
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 2 cut(s) 36, 44
HpyAV CCTTC 1 cut(s) 117
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 1 cut(s) 58
Hsp92II CATG 4 cut(s) 17, 62, 185, 385
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 5 cut(s) 82, 205, 232, 237, 241
Lsp1109I GCAGC 1 cut(s) 214
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 2 cut(s) 82, 197
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 144, 322
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 2 cut(s) 214, 259
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 1 cut(s) 305
MnlI CCTC 2 cut(s) 93, 279
Mox20I TGGCCA 1 cut(s) 242
Mph1103I ATGCAT 1 cut(s) 60
MscI TGGCCA 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 220
MvaI CCWGG 1 cut(s) 220
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 17, 62, 185, 385
NlaIV GGNNCC 2 cut(s) 105, 122
NsiI ATGCAT 1 cut(s) 60
PkrI GCNGC 2 cut(s) 120, 229
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 105, 122
PspPI GGNCC 1 cut(s) 71
PsrI GAACNNNNNNTAC 2 cut(s) 331, 363
PsuI RGATCY 1 cut(s) 103
SacI GAGCTC 1 cut(s) 144
SatI GCNGC 2 cut(s) 119, 228
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 71
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 220
SduI GDGCHC 2 cut(s) 144, 322
SetI ASST 2 cut(s) 144, 229
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 2 cut(s) 214, 259
SsiI CCGC 2 cut(s) 118, 368
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 218
TaaI ACNGT 1 cut(s) 238
TaqI TCGA 2 cut(s) 271, 322
TasI AATT 2 cut(s) 214, 259
TauI GCSGC 1 cut(s) 121
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 3 cut(s) 41, 75, 198
TspGWI ACGGA 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 273
XapI RAATTY 2 cut(s) 214, 259
Zsp2I ATGCAT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.