RLG00000024013

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000466
Physical Location & Seq
Reverse (-)
30883080 .. 30884002
923 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024013

Sequence Viewer

Length: 792 bp
ATGGAGTTCCATCAGTATGTTTTTGATGCTATGAAGATGATTATTGTGATTAGTGCGGTAATGGTGCAATGGTTCGGTGTCAGAGCCTGTCTTAAGTGGATATGGAAGAACTGGTTCATTTTTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTGACATGGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACTGGTTGAAGTCGTTGAAACCCCAGAGCCAATAGCAGAGGGTAATCAAGAACCGGTTGACGTCGATGCCTCTACATCTCTTCCTTCATCATCAGCTCCTAGGTGGAAGCACGATGTGTTTTTGAGTTTCAGGGGTGTAGACACTCGCAAAGGTATTGCATCCGAAATATACTATCGACTGCAAAACAGGAAAGGAATTAAAATTTTCATGGATGACCCAGACCTTCAAGTAGGGAATGTTATTTCTCCCACTCTCCTAACAGCAATTAAAGAATCAAGATTTGCAATTATTGTTCTCTCTCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGATACATTTGTGAATGCATGAAAGAAGACGAGAACAGAATTCTGCCACTTTTTTATTATGTGAATCCTACTGATGTACGATATCAGAAGAGCAGTTTCGGTGATGCTTTCACTAAGCATGAAAATTCTGGGCGATACGGATCAGAGGAGATGCGGCAGTGGAGGGTTGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGGACACAAAGAATTATAAGTAA

Protein Analysis

264

Amino Acids

30.61

Weight (kDa)

5.56

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 113 - 262 2e-44 TIR domain
TIR_2 PF13676 116 - 210 8.2e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AatII GACGTC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 369
AciI CCGC 2 cut(s) 56, 724
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 4 cut(s) 432, 609, 694, 757
AcyI GRCGYC 1 cut(s) 291
AdeI CACNNNGTG 2 cut(s) 346, 555
AfaI GTAC 1 cut(s) 648
AfiI CCNNNNNNNGG 1 cut(s) 159
AflII CTTAAG 1 cut(s) 92
AgeI ACCGGT 1 cut(s) 283
AgsI TTSAA 4 cut(s) 197, 239, 248, 458
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
AlwI GGATC 1 cut(s) 718
AlwNI CAGNNNCTG 1 cut(s) 87
ApoI RAATTY 4 cut(s) 432, 609, 694, 757
AsiGI ACCGGT 1 cut(s) 283
Asp700I GAANNNNTTC 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 139, 683
AvrII CCTAGG 1 cut(s) 329
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BbsI GAAGAC 1 cut(s) 603
BccI CCATC 2 cut(s) 18, 762
BfaI CTAG 1 cut(s) 330
BfrI CTTAAG 1 cut(s) 92
BisI GCNGC 1 cut(s) 725
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 1 cut(s) 726
BmsI GCATC 5 cut(s) 16, 286, 398, 664, 711
BpiI GAAGAC 1 cut(s) 603
BsaBI GATNNNNATC 1 cut(s) 709
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 329
BsaWI WCCGGW 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 3 cut(s) 116, 153, 237
Bse3DI GCAATG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 709
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 3 cut(s) 389, 448, 773
BseJI GATNNNNATC 1 cut(s) 709
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 74
BseNI ACTGG 3 cut(s) 116, 153, 237
BseRI GAGGAG 1 cut(s) 731
BshTI ACCGGT 1 cut(s) 283
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 785
BsmI GAATGC 1 cut(s) 590
Bsp143I GATC 1 cut(s) 710
BspACI CCGC 2 cut(s) 56, 724
BspPI GGATC 1 cut(s) 718
BspQI GCTCTTC 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 3 cut(s) 116, 153, 237
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 1 cut(s) 710
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 329
Bst6I CTCTTC 4 cut(s) 167, 315, 550, 653
BstACI GRCGYC 1 cut(s) 291
BstAFI CTTAAG 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 569, 684
BstF5I GGATG 3 cut(s) 389, 448, 773
BstKTI GATC 1 cut(s) 713
BstMBI GATC 1 cut(s) 710
BstV2I GAAGAC 1 cut(s) 603
BtsCI GGATG 3 cut(s) 389, 448, 773
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 2 cut(s) 190, 734
CaiI CAGNNNCTG 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 283
Csp6I GTAC 1 cut(s) 647
CspAI ACCGGT 1 cut(s) 283
CviAII CATG 5 cut(s) 138, 190, 439, 589, 689
CviJI RGCY 5 cut(s) 86, 152, 208, 259, 326
CviKI_1 RGCY 5 cut(s) 86, 152, 208, 259, 326
CviQI GTAC 1 cut(s) 647
DdeI CTNAG 2 cut(s) 569, 684
DpnI GATC 1 cut(s) 712
DpnII GATC 1 cut(s) 710
DraI TTTAAA 1 cut(s) 744
DraIII CACNNNGTG 2 cut(s) 346, 555
Eam1104I CTCTTC 4 cut(s) 167, 315, 550, 653
EarI CTCTTC 4 cut(s) 167, 315, 550, 653
Eco130I CCWWGG 1 cut(s) 329
Eco32I GATATC 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 609
EcoRV GATATC 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 5 cut(s) 141, 193, 442, 592, 692
FaqI GGGAC 1 cut(s) 785
FatI CATG 5 cut(s) 137, 189, 438, 588, 688
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 725
FokI GGATG 3 cut(s) 376, 455, 780
Fsp4HI GCNGC 1 cut(s) 725
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 725
HapII CCGG 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 5 cut(s) 141, 193, 442, 592, 692
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 2 cut(s) 503, 634
HpaII CCGG 1 cut(s) 284
HphI GGTGA 2 cut(s) 139, 683
Hpy166II GTNNAC 2 cut(s) 289, 370
Hpy188I TCNGA 5 cut(s) 83, 217, 394, 657, 715
Hpy188III TCNNGA 4 cut(s) 227, 278, 507, 765
Hpy8I GTNNAC 2 cut(s) 289, 370
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 324, 464
HpyCH4IV ACGT 2 cut(s) 144, 291
HpyCH4V TGCA 5 cut(s) 67, 389, 412, 515, 588
HpyF3I CTNAG 2 cut(s) 569, 684
HpySE526I ACGT 2 cut(s) 144, 291
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 5 cut(s) 141, 193, 442, 592, 692
Kzo9I GATC 1 cut(s) 710
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 1 cut(s) 331
LweI GCATC 5 cut(s) 16, 286, 398, 664, 711
MaeI CTAG 1 cut(s) 330
MaeII ACGT 2 cut(s) 144, 291
MaeIII GTNAC 2 cut(s) 133, 185
MalI GATC 1 cut(s) 712
MboI GATC 1 cut(s) 710
MboII GAAGA 7 cut(s) 46, 118, 184, 302, 537, 608, 670
MluCI AATT 8 cut(s) 426, 432, 495, 516, 609, 694, 757, 781
MnlI CCTC 7 cut(s) 211, 262, 310, 553, 556, 709, 726
Mph1103I ATGCAT 1 cut(s) 590
MroXI GAANNNNTTC 1 cut(s) 113
MseI TTAA 4 cut(s) 93, 429, 498, 743
MslI CAYNNNNRTG 2 cut(s) 15, 122
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 590
NdeII GATC 1 cut(s) 710
NlaIII CATG 5 cut(s) 141, 193, 442, 592, 692
NmuCI GTSAC 2 cut(s) 133, 185
NsiI ATGCAT 1 cut(s) 590
PciSI GCTCTTC 1 cut(s) 653
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 503, 634
PinAI ACCGGT 1 cut(s) 283
PkrI GCNGC 1 cut(s) 726
PsiI TTATAA 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 87
RsaI GTAC 1 cut(s) 648
RsaNI GTAC 1 cut(s) 647
RseI CAYNNNNRTG 2 cut(s) 15, 122
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 4 cut(s) 93, 429, 498, 743
SatI GCNGC 1 cut(s) 725
Sau3AI GATC 1 cut(s) 710
SetI ASST 7 cut(s) 147, 182, 294, 328, 335, 385, 456
SfaNI GCATC 5 cut(s) 16, 286, 398, 664, 711
SmiMI CAYNNNNRTG 2 cut(s) 15, 122
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 8 cut(s) 426, 432, 495, 516, 609, 694, 757, 781
SsiI CCGC 2 cut(s) 56, 724
SspMI CTAG 1 cut(s) 330
StyI CCWWGG 1 cut(s) 329
TaiI ACGT 2 cut(s) 147, 294
TaqI TCGA 2 cut(s) 294, 406
TasI AATT 8 cut(s) 426, 432, 495, 516, 609, 694, 757, 781
TauI GCSGC 1 cut(s) 727
TfiI GAWTC 2 cut(s) 503, 634
Tru1I TTAA 4 cut(s) 93, 429, 498, 743
Tru9I TTAA 4 cut(s) 93, 429, 498, 743
TscAI CASTG 2 cut(s) 190, 734
TseFI GTSAC 2 cut(s) 133, 185
Tsp45I GTSAC 2 cut(s) 133, 185
TspDTI ATGAA 6 cut(s) 47, 106, 306, 427, 605, 705
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 2 cut(s) 190, 734
Vha464I CTTAAG 1 cut(s) 92
XapI RAATTY 4 cut(s) 432, 609, 694, 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 556
XmaJI CCTAGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 330
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.