Rroxscaffold_1G00003530

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000466
Physical Location & Seq
Forward (+)
4344624 .. 4348520
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003530.1

Sequence Viewer

Length: 1014 bp
ATGGGCGAAGCTATAACCCCGGACCCGAGTGGTTTTATTTTTGGGCCGCAGGTATCGGCACACCGGCTTGAACCCACCAAAGGATCTTGCCAAAAATGCTTTTGGGCTCAAACAGCATCAAGCACCTCAAGCGCCTCTCATGATGTGTTTTTGAGCTTCAGGGTTGAAGACACTCTAAAGGGTGTTGCAGATTATTTATACCATGAATTGCAAAAGAGAGGAATACAAACATTCATGCATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCGTCAAGTCTCCTAGCAGCAATCAAGGAATCGAGGTGTGCAATTGTTGTGTTGTCGCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAATTTATACAAATACTTCAATGCTCCGTAGCCAAGAAGACAATGGTTCTGCCAATTTTCTATAATGTGGATCCCTCTGATGTGAGGAATCAAAGAGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAAAAGTTTAGGCAAGGCAATGACAATGTAGAGAAGGCGAAGAAGTGGAGAGTTGCTTTAACAAAAGTGGCCAATATCTCTGGGTTTGATTCAAACAAATATAGGGCGCAGATTCCTGACTTGAATTCCTCTCAAGGTAGGTGTCATTCTTCAATTCCATCACAGCGGGAAATCTCATATTCCAAAGCGCCAAAATCAAAACATGCCGCGCTAAGTTTTGATCCAAAACAACTCTATATAAAGCCACAAAACTTGCAAGACCGGACTCCAAAATTTGCGATCGTTCTCATATCCGAGTTTTCTTCTGCTGCTAAAAAGGTTTTGCAATCGTTCTTGTCGTTGAGTTTTCTTCCGGGGACCCTCTCTACTACTGCTGAAAAGGTTAGTCTTTTTGCTCTGTCTCCTGATTCTTTTCTTTTGCTTTCTATAGTCTTCCGTGTGCCTTGTTCTTCATCTATATCTGCATCACACAAATCATCAAGAGCATTATACAAATCATCAAGAGCATTATGTAGCTTCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

337

Amino Acids

37.26

Weight (kDa)

9.38

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 47 - 198 9.4e-46 TIR domain
TIR_2 PF13676 49 - 146 5e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccII CGCG 1 cut(s) 698
AciI CCGC 3 cut(s) 47, 655, 696
AclWI GGATC 4 cut(s) 91, 425, 438, 704
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 3 cut(s) 362, 613, 761
AcuI CTGAAG 2 cut(s) 142, 236
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 6 cut(s) 71, 167, 380, 582, 613, 642
AjuI GAANNNNNNNTTGG 2 cut(s) 322, 354
AluBI AGCT 3 cut(s) 11, 156, 1005
AluI AGCT 3 cut(s) 11, 156, 1005
Alw26I GTCTC 2 cut(s) 284, 894
AlwI GGATC 4 cut(s) 91, 425, 438, 704
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 2 cut(s) 44, 558
ApeKI GCWGC 2 cut(s) 287, 797
ApoI RAATTY 3 cut(s) 362, 613, 761
AspLEI GCGC 4 cut(s) 134, 598, 679, 700
AspS9I GGNCC 3 cut(s) 22, 44, 846
AsuC2I CCSGG 2 cut(s) 20, 843
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 2 cut(s) 22, 846
BalI TGGCCA 1 cut(s) 560
BamHI GGATCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 3 cut(s) 174, 404, 913
BbvI GCAGC 2 cut(s) 299, 784
BccI CCATC 1 cut(s) 655
BcnI CCSGG 2 cut(s) 20, 843
BcoDI GTCTC 2 cut(s) 284, 894
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 2 cut(s) 135, 680
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 4 cut(s) 47, 288, 696, 798
BlsI GCNGC 4 cut(s) 48, 289, 697, 799
Bme1390I CCNGG 2 cut(s) 20, 843
Bme18I GGWCC 2 cut(s) 22, 846
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 22, 44, 846
BmiI GGNNCC 4 cut(s) 24, 432, 847, 848
BmrFI CCNGG 2 cut(s) 20, 843
BmsI GCATC 3 cut(s) 125, 252, 962
BpiI GAAGAC 3 cut(s) 174, 404, 913
BpuEI CTTGAG 2 cut(s) 112, 606
BpuMI CCSGG 2 cut(s) 20, 843
BsaJI CCNNGG 3 cut(s) 18, 244, 842
BsaWI WCCGGW 1 cut(s) 750
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 514
BseDI CCNNGG 3 cut(s) 18, 244, 842
BseGI GGATG 2 cut(s) 267, 364
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 514
BseXI GCAGC 2 cut(s) 299, 784
Bsh1236I CGCG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 771
BshFI GGCC 2 cut(s) 46, 560
BsiEI CGRYCG 1 cut(s) 771
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 4 cut(s) 20, 64, 751, 842
BslFI GGGAC 1 cut(s) 859
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 2 cut(s) 284, 894
BsmFI GGGAC 1 cut(s) 859
BsnI GGCC 2 cut(s) 46, 560
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 4 cut(s) 83, 430, 709, 768
BspACI CCGC 3 cut(s) 47, 655, 696
BspANI GGCC 2 cut(s) 46, 560
BspFNI CGCG 1 cut(s) 698
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 4 cut(s) 24, 432, 847, 848
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 4 cut(s) 91, 425, 438, 704
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 63
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 3 cut(s) 18, 244, 842
BssMI GATC 4 cut(s) 83, 430, 709, 768
BssT1I CCWWGG 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 262
BstDEI CTNAG 2 cut(s) 480, 701
BstF5I GGATG 2 cut(s) 267, 364
BstFNI CGCG 1 cut(s) 698
BstH2I RGCGCY 2 cut(s) 135, 680
BstHHI GCGC 4 cut(s) 134, 598, 679, 700
BstKTI GATC 4 cut(s) 86, 433, 712, 771
BstMAI GTCTC 2 cut(s) 284, 894
BstMBI GATC 4 cut(s) 83, 430, 709, 768
BstMCI CGRYCG 1 cut(s) 771
BstMWI GCNNNNNNNGC 4 cut(s) 96, 113, 129, 262
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 2 cut(s) 18, 841
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 1 cut(s) 698
BstV1I GCAGC 2 cut(s) 299, 784
BstV2I GAAGAC 3 cut(s) 174, 404, 913
BstX2I RGATCY 2 cut(s) 83, 430
BstYI RGATCY 2 cut(s) 83, 430
BsuRI GGCC 2 cut(s) 46, 560
BtsCI GGATG 2 cut(s) 267, 364
BveI ACCTGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 139
CfoI GCGC 4 cut(s) 134, 598, 679, 700
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 22, 44, 846
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 7 cut(s) 140, 203, 235, 239, 348, 485, 692
DdeI CTNAG 2 cut(s) 480, 701
DpnI GATC 4 cut(s) 85, 432, 711, 770
DpnII GATC 4 cut(s) 83, 430, 709, 768
EaeI YGGCCR 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 22, 846
Eco57I CTGAAG 2 cut(s) 142, 236
Eco88I CYCGRG 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 846
EcoRI GAATTC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 7 cut(s) 143, 206, 238, 242, 351, 488, 695
FaqI GGGAC 1 cut(s) 859
FatI CATG 7 cut(s) 139, 202, 234, 238, 347, 484, 691
FauI CCCGC 1 cut(s) 648
Fnu4HI GCNGC 4 cut(s) 47, 288, 696, 798
FokI GGATG 2 cut(s) 274, 371
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 4 cut(s) 47, 288, 696, 798
FspBI CTAG 1 cut(s) 284
GlaI GCGC 4 cut(s) 133, 597, 678, 699
GluI GCNGC 4 cut(s) 47, 288, 696, 798
HaeII RGCGCY 2 cut(s) 135, 680
HaeIII GGCC 2 cut(s) 46, 560
HapII CCGG 4 cut(s) 20, 64, 751, 842
HhaI GCGC 4 cut(s) 134, 598, 679, 700
Hin1II CATG 7 cut(s) 143, 206, 238, 242, 351, 488, 695
Hin6I GCGC 4 cut(s) 132, 596, 677, 698
HinP1I GCGC 4 cut(s) 132, 596, 677, 698
HinfI GANTC 6 cut(s) 299, 448, 578, 601, 754, 896
HpaII CCGG 4 cut(s) 20, 64, 751, 842
Hpy188I TCNGA 2 cut(s) 439, 784
Hpy188III TCNNGA 5 cut(s) 140, 605, 893, 969, 990
HpyAV CCTTC 2 cut(s) 484, 517
HpyCH4V TGCA 8 cut(s) 188, 211, 238, 311, 467, 745, 814, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 113, 129, 262
HpyF3I CTNAG 2 cut(s) 480, 701
Hsp92II CATG 7 cut(s) 143, 206, 238, 242, 351, 488, 695
HspAI GCGC 4 cut(s) 132, 596, 677, 698
KflI GGGWCCC 1 cut(s) 846
Kzo9I GATC 4 cut(s) 83, 430, 709, 768
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 9 cut(s) 33, 35, 77, 145, 555, 618, 764, 855, 906
Lsp1109I GCAGC 2 cut(s) 299, 784
LweI GCATC 3 cut(s) 125, 252, 962
MaeI CTAG 1 cut(s) 284
MalI GATC 4 cut(s) 85, 432, 711, 770
MboI GATC 4 cut(s) 83, 430, 709, 768
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 2 cut(s) 83, 430
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 7 cut(s) 206, 312, 362, 414, 613, 642, 761
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 1 cut(s) 748
MnlI CCTC 9 cut(s) 136, 145, 212, 297, 438, 445, 449, 628, 860
Mox20I TGGCCA 1 cut(s) 560
Mph1103I ATGCAT 1 cut(s) 240
MscI TGGCCA 1 cut(s) 560
MseI TTAA 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 4 cut(s) 20, 64, 751, 842
MspR9I CCNGG 2 cut(s) 20, 843
MunI CAATTG 1 cut(s) 312
MvnI CGCG 1 cut(s) 698
MwoI GCNNNNNNNGC 4 cut(s) 96, 113, 129, 262
NciI CCSGG 2 cut(s) 20, 843
NdeII GATC 4 cut(s) 83, 430, 709, 768
NlaIII CATG 7 cut(s) 143, 206, 238, 242, 351, 488, 695
NlaIV GGNNCC 4 cut(s) 24, 432, 847, 848
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 1 cut(s) 695
PagI TCATGA 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 824
PfeI GAWTC 5 cut(s) 299, 448, 578, 601, 896
PkrI GCNGC 4 cut(s) 48, 289, 697, 799
Ple19I CGATCG 1 cut(s) 771
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 846
PspN4I GGNNCC 4 cut(s) 24, 432, 847, 848
PspPI GGNCC 3 cut(s) 22, 44, 846
PspPPI RGGWCCY 1 cut(s) 846
PsuI RGATCY 2 cut(s) 83, 430
PvuI CGATCG 1 cut(s) 771
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 4 cut(s) 47, 288, 696, 798
Sau3AI GATC 4 cut(s) 83, 430, 709, 768
Sau96I GGNCC 3 cut(s) 22, 44, 846
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 2 cut(s) 20, 843
SduI GDGCHC 1 cut(s) 109
SfaNI GCATC 3 cut(s) 125, 252, 962
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 22, 846
SmlI CTYRAG 2 cut(s) 127, 621
SmoI CTYRAG 2 cut(s) 127, 621
Sse9I AATT 7 cut(s) 206, 312, 362, 414, 613, 642, 761
SsiI CCGC 3 cut(s) 47, 655, 696
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 18, 841
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 1 cut(s) 302
TasI AATT 7 cut(s) 206, 312, 362, 414, 613, 642, 761
TauI GCSGC 2 cut(s) 49, 698
TfiI GAWTC 5 cut(s) 299, 448, 578, 601, 896
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TseI GCWGC 2 cut(s) 287, 797
TspDTI ATGAA 5 cut(s) 219, 223, 375, 501, 930
TspGWI ACGGA 2 cut(s) 376, 914
VpaK11BI GGWCC 2 cut(s) 22, 846
XapI RAATTY 3 cut(s) 362, 613, 761
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 400
XspI CTAG 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.