FvH4_3g44041

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000466
Physical Location & Seq
Reverse (-)
36754931 .. 36758779
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44041.t1

Sequence Viewer

Length: 855 bp
ATGGACGACTCAGATCTTCAAGTAGGGGATGCTATTTCTACCTCTCTCTTAGAGGCAATTGAAGGATCAAAGTTGGCAATTGTTGTTCTGTCGCAAAATTTTGCTGCTTTTACTTTGTGTTTGGAGGAACTCACAAAGATTTGTCAGTTCATGGAATATGGCCCCAGAATTCTTCCAGTTTTTTATCATGTCAACCCTAGTGATGTGAGATATCAAAAGGGAAGTTTTCAAGATGCTTTCACCAAATATGAAAAGTCTGGGCGATACAAATCAGAGAAGGTGAAGCAGTGGAGAGATGCTTTAAGAAAAGTGGCCAGTTTGTCTGGGTGGCTTGCACAAGATTACAACACTGAGACAGGAGTTTTCGAAGCCATTATGAAATCTGTGTACACGGCAGTTTCGGGTCTGTGTTTGAGGGAATTTCTAATGGTGGATGCTTCTTTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCATGGGGAGGCAAAGAGTTCTTCAACTTGAACAGGGAAATTGCTCTTTTGAGTCAGTTTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGGATCAAAGCTCTATATTTTTCTGGAACTTGTAACCAAAGGCTCCCTTCAAAAGCTATATCAAACGTACCATCTTACAGATTCGCATGCCTCTGTATACACGAGACAGATCTTGCAAGGTCTGAAGTATCTTCACGACCGAAAAGTGATTCACAGGGACATTAAATGCTCAAATATTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCTGACTTTGGATTGGCAAAGGTTGTCTTCCCTTCCCCAAATTCAATTGTATTTTATAGTTTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

285

Amino Acids

32.33

Weight (kDa)

8.44

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 1 - 67 8.2e-06 TIR domain
TIR PF01582 2 - 133 2.5e-29 TIR domain
PK_Tyr_Ser-Thr PF07714 165 - 271 2.6e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 168 - 273 1.6e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 675
AclWI GGATC 3 cut(s) 73, 589, 781
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 4 cut(s) 97, 168, 419, 826
AcuI CTGAAG 1 cut(s) 722
AfaI GTAC 2 cut(s) 389, 647
AgsI TTSAA 8 cut(s) 20, 62, 230, 502, 508, 539, 629, 831
AluBI AGCT 3 cut(s) 589, 634, 784
AluI AGCT 3 cut(s) 589, 634, 784
Alw26I GTCTC 2 cut(s) 347, 676
AlwI GGATC 3 cut(s) 73, 589, 781
AoxI GGCC 2 cut(s) 160, 312
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 4 cut(s) 97, 168, 419, 826
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 232, 292
AsuII TTCGAA 1 cut(s) 366
BalI TGGCCA 1 cut(s) 314
BauI CACGAG 1 cut(s) 679
BbsI GAAGAC 1 cut(s) 805
BbvI GCAGC 1 cut(s) 91
BccI CCATC 2 cut(s) 572, 657
BceAI ACGGC 1 cut(s) 408
BclI TGATCA 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 347, 676
BfaI CTAG 1 cut(s) 198
BglII AGATCT 2 cut(s) 13, 687
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 163, 622
BmsI GCATC 4 cut(s) 19, 223, 286, 424
BpiI GAAGAC 1 cut(s) 805
Bpu14I TTCGAA 1 cut(s) 366
BsaBI GATNNNNATC 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 176, 315
Bse8I GATNNNNATC 1 cut(s) 268
BseGI GGATG 3 cut(s) 34, 439, 583
BseJI GATNNNNATC 1 cut(s) 268
BseMII CTCAG 2 cut(s) 24, 342
BseNI ACTGG 2 cut(s) 176, 315
BseXI GCAGC 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 718
BshFI GGCC 2 cut(s) 162, 314
BsiEI CGRYCG 1 cut(s) 718
BslFI GGGAC 1 cut(s) 749
BsmAI GTCTC 2 cut(s) 347, 676
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 2 cut(s) 162, 314
Bsp119I TTCGAA 1 cut(s) 366
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 6 cut(s) 13, 65, 468, 581, 687, 773
BspANI GGCC 2 cut(s) 162, 314
BspCNI CTCAG 2 cut(s) 23, 343
BspLI GGNNCC 2 cut(s) 163, 622
BspPI GGATC 3 cut(s) 73, 589, 781
BspT104I TTCGAA 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 176, 315
BssMI GATC 6 cut(s) 13, 65, 468, 581, 687, 773
BssNAI GTATAC 1 cut(s) 676
BssSI CACGAG 1 cut(s) 679
Bst1107I GTATAC 1 cut(s) 676
Bst2BI CACGAG 1 cut(s) 679
BstAUI TGTACA 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 366
BstC8I GCNNGC 4 cut(s) 333, 666, 765, 786
BstDEI CTNAG 3 cut(s) 10, 49, 351
BstF5I GGATG 3 cut(s) 34, 439, 583
BstKTI GATC 6 cut(s) 16, 68, 471, 584, 690, 776
BstMAI GTCTC 2 cut(s) 347, 676
BstMBI GATC 6 cut(s) 13, 65, 468, 581, 687, 773
BstMCI CGRYCG 1 cut(s) 718
BstNSI RCATGY 2 cut(s) 668, 767
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 1 cut(s) 805
BstX2I RGATCY 3 cut(s) 13, 687, 773
BstYI RGATCY 3 cut(s) 13, 687, 773
BstZ17I GTATAC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 162, 314
BtsCI GGATG 3 cut(s) 34, 439, 583
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 2 cut(s) 293, 348
Cac8I GCNNGC 4 cut(s) 333, 666, 765, 786
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 2 cut(s) 388, 646
CviAII CATG 6 cut(s) 151, 188, 480, 542, 665, 764
CviJI RGCY 8 cut(s) 162, 314, 331, 371, 589, 621, 634, 784
CviKI_1 RGCY 8 cut(s) 162, 314, 331, 371, 589, 621, 634, 784
CviQI GTAC 2 cut(s) 388, 646
DdeI CTNAG 3 cut(s) 10, 49, 351
DpnI GATC 6 cut(s) 15, 67, 470, 583, 689, 775
DpnII GATC 6 cut(s) 13, 65, 468, 581, 687, 773
EaeI YGGCCR 1 cut(s) 312
Eco32I GATATC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 168
EcoRV GATATC 1 cut(s) 212
FaeI CATG 6 cut(s) 154, 191, 483, 545, 668, 767
FalI AAGNNNNNCTT 4 cut(s) 609, 641, 797, 829
FaqI GGGAC 1 cut(s) 749
FatI CATG 6 cut(s) 150, 187, 479, 541, 664, 763
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 3 cut(s) 41, 446, 590
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 1 cut(s) 198
GluI GCNGC 1 cut(s) 105
HaeIII GGCC 2 cut(s) 162, 314
Hin1II CATG 6 cut(s) 154, 191, 483, 545, 668, 767
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 4 cut(s) 8, 529, 659, 727
HphI GGTGA 2 cut(s) 232, 292
Hpy166II GTNNAC 4 cut(s) 193, 388, 390, 676
Hpy188I TCNGA 3 cut(s) 13, 274, 702
Hpy188III TCNNGA 3 cut(s) 230, 602, 713
Hpy8I GTNNAC 4 cut(s) 193, 388, 390, 676
HpyAV CCTTC 4 cut(s) 56, 271, 635, 828
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 3 cut(s) 335, 694, 763
HpyF3I CTNAG 3 cut(s) 10, 49, 351
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 6 cut(s) 154, 191, 483, 545, 668, 767
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 6 cut(s) 13, 65, 468, 581, 687, 773
LmnI GCTCC 1 cut(s) 626
LpnPI CCDG 9 cut(s) 178, 189, 243, 309, 328, 342, 496, 587, 718
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 4 cut(s) 19, 223, 286, 424
MaeI CTAG 1 cut(s) 198
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 1 cut(s) 610
MalI GATC 6 cut(s) 15, 67, 470, 583, 689, 775
MboI GATC 6 cut(s) 13, 65, 468, 581, 687, 773
MboII GAAGA 5 cut(s) 8, 164, 490, 701, 805
MfeI CAATTG 3 cut(s) 57, 78, 831
MflI RGATCY 3 cut(s) 13, 687, 773
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 8 cut(s) 57, 78, 97, 168, 419, 516, 826, 831
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 2 cut(s) 2, 538
MnlI CCTC 6 cut(s) 46, 52, 118, 408, 479, 679
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 2 cut(s) 302, 741
MslI CAYNNNNRTG 1 cut(s) 768
Msp20I TGGCCA 1 cut(s) 314
MunI CAATTG 3 cut(s) 57, 78, 831
NdeII GATC 6 cut(s) 13, 65, 468, 581, 687, 773
NlaIII CATG 6 cut(s) 154, 191, 483, 545, 668, 767
NlaIV GGNNCC 2 cut(s) 163, 622
NspI RCATGY 2 cut(s) 668, 767
NspV TTCGAA 1 cut(s) 366
PaeI GCATGC 2 cut(s) 668, 767
PfeI GAWTC 2 cut(s) 659, 727
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 2 cut(s) 2, 537
PpsI GAGTC 2 cut(s) 2, 537
PspN4I GGNNCC 2 cut(s) 163, 622
PspPI GGNCC 1 cut(s) 161
PsuI RGATCY 3 cut(s) 13, 687, 773
RsaI GTAC 2 cut(s) 389, 647
RsaNI GTAC 2 cut(s) 388, 646
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 2 cut(s) 302, 741
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 6 cut(s) 13, 65, 468, 581, 687, 773
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 2 cut(s) 2, 538
SetI ASST 8 cut(s) 44, 282, 591, 636, 647, 700, 786, 810
SfaNI GCATC 4 cut(s) 19, 223, 286, 424
SfuI TTCGAA 1 cut(s) 366
SmiMI CAYNNNNRTG 1 cut(s) 768
SphI GCATGC 2 cut(s) 668, 767
Sse9I AATT 8 cut(s) 57, 78, 97, 168, 419, 516, 826, 831
SspI AATATT 1 cut(s) 754
SspMI CTAG 1 cut(s) 198
TaiI ACGT 1 cut(s) 647
TaqI TCGA 1 cut(s) 366
TaqII GACCGA 1 cut(s) 732
TasI AATT 8 cut(s) 57, 78, 97, 168, 419, 516, 826, 831
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 2 cut(s) 659, 727
Tru1I TTAA 2 cut(s) 302, 741
Tru9I TTAA 2 cut(s) 302, 741
TscAI CASTG 2 cut(s) 293, 355
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 4 cut(s) 139, 264, 392, 840
TspRI CASTG 2 cut(s) 293, 355
XapI RAATTY 4 cut(s) 97, 168, 419, 826
XceI RCATGY 2 cut(s) 668, 767
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.