MD12G1125500.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000466
Physical Location & Seq
Reverse (-)
20089370 .. 20090816
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1125500.v1.1.491

Sequence Viewer

Length: 234 bp
ATGCAATGCAGAAAAGACATGGGGCAAGAAGTCGTGCCAATCTTTCACAACGTGGATCCGTCTGAGGTGCGCCACCAAACGGGAAGGTTCTTGCTGGCCGTGAGGAATGTAACGGAGCATGAAGAAGTTTGTTCTGAGGATAGGAACAACATAAACCGGTGGAGGGCTGCTTTGAGAGAGGTGGCTGGTCTTAAGGGCTGGGATTTACAACATTGGTGGAGGCAGAACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

78

Amino Acids

9.21

Weight (kDa)

6.9

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 1 - 72 5.1e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 50, 63
AcoI YGGCCR 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 79, 163
AflII CTTAAG 1 cut(s) 191
AgeI ACCGGT 1 cut(s) 156
AlwI GGATC 2 cut(s) 50, 63
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 167
AsiGI ACCGGT 1 cut(s) 156
AspLEI GCGC 1 cut(s) 72
BamHI GGATCC 1 cut(s) 55
BbvI GCAGC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 156
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 163
Bse118I RCCGGY 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 11
BseLI CCNNNNNNNGG 2 cut(s) 79, 163
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 2 cut(s) 54, 126
BseXI GCAGC 1 cut(s) 154
BseYI CCCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 98
BshTI ACCGGT 1 cut(s) 156
BsiSI CCGG 1 cut(s) 157
BslI CCNNNNNNNGG 2 cut(s) 79, 163
BsnI GGCC 1 cut(s) 98
Bsp143I GATC 1 cut(s) 55
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 55, 127
BspLI GGNNCC 1 cut(s) 57
BspPI GGATC 2 cut(s) 50, 63
BspTI CTTAAG 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 156
BssAI RCCGGY 1 cut(s) 156
BssMI GATC 1 cut(s) 55
BstAFI CTTAAG 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 63, 135
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 1 cut(s) 58
BstMBI GATC 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuRI GGCC 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 156
CspAI ACCGGT 1 cut(s) 156
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviAII CATG 2 cut(s) 19, 119
CviJI RGCY 4 cut(s) 98, 167, 185, 198
CviKI_1 RGCY 4 cut(s) 98, 167, 185, 198
DdeI CTNAG 2 cut(s) 63, 135
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
DraIII CACNNNGTG 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 96
FaeI CATG 2 cut(s) 22, 122
FaiI YATR 3 cut(s) 20, 120, 152
FatI CATG 2 cut(s) 18, 118
Fnu4HI GCNGC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 168
GlaI GCGC 1 cut(s) 71
GluI GCNGC 1 cut(s) 168
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 157
HhaI GCGC 1 cut(s) 72
Hin1II CATG 2 cut(s) 22, 122
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HpaII CCGG 1 cut(s) 157
Hpy188I TCNGA 2 cut(s) 64, 136
HpyAV CCTTC 1 cut(s) 78
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 4, 9
HpyF3I CTNAG 2 cut(s) 63, 135
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 2 cut(s) 22, 122
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 4 cut(s) 80, 170, 171, 184
Lsp1109I GCAGC 1 cut(s) 154
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 1 cut(s) 134
MflI RGATCY 1 cut(s) 55
MnlI CCTC 6 cut(s) 58, 96, 130, 156, 172, 213
MseI TTAA 1 cut(s) 192
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 1 cut(s) 157
NdeII GATC 1 cut(s) 55
NlaIII CATG 2 cut(s) 22, 122
NlaIV GGNNCC 1 cut(s) 57
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 57
PsuI RGATCY 1 cut(s) 55
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 1 cut(s) 55
SetI ASST 4 cut(s) 54, 69, 89, 183
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
TaiI ACGT 1 cut(s) 54
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 135
TspGWI ACGGA 2 cut(s) 48, 128
Vha464I CTTAAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.