RLG00000030320

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000466
Physical Location & Seq
Reverse (-)
59559940 .. 59560823
884 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030320

Sequence Viewer

Length: 585 bp
ATGGCATCAACCTCTTCTCGTCGTCGTCAATGGAAATACAATGTCTTTCTAAGTTTCAGGGGGGATGATACCCGTGGGAGTTTTGCCTCTCTTCTGTATGCTGCACTAAATCGGAAAGGAATTCTCACCTTTCGGGATGACCGATGGCTTGACAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTGTCGGAGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTTGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTCCCCATCTTCTACTTTGTGGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTCAGCTCAAATGGGAACCCCAAATAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGACAGACTAAATGCGTGGAGAGCTGCTTTGACTGACGTCACCAATCTTTCTGGCTGGGATTTCAAGAATTATCCATATTTTACAAAACTAATTGACGAAATTGTTAGTCATATACTAAAGAAGTCCGTGTATACATCTTCAATGTTGACAAGGGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

195

Amino Acids

22.7

Weight (kDa)

9.2

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 185 4.5e-50 TIR domain
TIR_2 PF13676 15 - 111 1.4e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 345
AccI GTMKAC 1 cut(s) 554
AciI CCGC 1 cut(s) 343
AclWI GGATC 2 cut(s) 323, 336
AcsI RAATTY 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 459
AgsI TTSAA 3 cut(s) 278, 487, 564
AluBI AGCT 3 cut(s) 185, 367, 446
AluI AGCT 3 cut(s) 185, 367, 446
AlwI GGATC 2 cut(s) 323, 336
AoxI GGCC 2 cut(s) 170, 295
ApeKI GCWGC 3 cut(s) 101, 185, 446
ApoI RAATTY 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 118, 454
BamHI GGATCC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 88, 172, 433
BccI CCATC 3 cut(s) 138, 286, 320
BfaI CTAG 1 cut(s) 201
BglII AGATCT 1 cut(s) 203
BisI GCNGC 4 cut(s) 102, 186, 344, 447
BlsI GCNGC 4 cut(s) 103, 187, 345, 448
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 3 cut(s) 330, 347, 378
BmsI GCATC 1 cut(s) 14
BoxI GACNNNNGTC 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 73
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 3 cut(s) 70, 142, 262
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 3 cut(s) 88, 172, 433
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 2 cut(s) 172, 297
BshNI GGYRCC 1 cut(s) 345
BsnI GGCC 2 cut(s) 172, 297
Bsp143I GATC 2 cut(s) 203, 328
BspACI CCGC 1 cut(s) 343
BspANI GGCC 2 cut(s) 172, 297
BspLI GGNNCC 3 cut(s) 330, 347, 378
BspPI GGATC 2 cut(s) 323, 336
BspT107I GGYRCC 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 2 cut(s) 203, 328
BssNAI GTATAC 1 cut(s) 555
BssNI GRCGYC 1 cut(s) 459
Bst1107I GTATAC 1 cut(s) 555
Bst6I CTCTTC 2 cut(s) 19, 96
BstACI GRCGYC 1 cut(s) 459
BstDEI CTNAG 1 cut(s) 50
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 3 cut(s) 70, 142, 262
BstKTI GATC 2 cut(s) 206, 331
BstMBI GATC 2 cut(s) 203, 328
BstMWI GCNNNNNNNGC 2 cut(s) 352, 443
BstPAI GACNNNNGTC 1 cut(s) 458
BstV1I GCAGC 3 cut(s) 88, 172, 433
BstX2I RGATCY 2 cut(s) 203, 328
BstYI RGATCY 2 cut(s) 203, 328
BstZ17I GTATAC 1 cut(s) 555
BsuRI GGCC 2 cut(s) 172, 297
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 3 cut(s) 70, 142, 262
Cfr13I GGNCC 1 cut(s) 295
CviAII CATG 3 cut(s) 246, 283, 401
CviJI RGCY 8 cut(s) 148, 172, 185, 297, 367, 446, 477, 578
CviKI_1 RGCY 8 cut(s) 148, 172, 185, 297, 367, 446, 477, 578
DdeI CTNAG 1 cut(s) 50
DpnI GATC 2 cut(s) 205, 330
DpnII GATC 2 cut(s) 203, 328
Eam1104I CTCTTC 2 cut(s) 19, 96
EarI CTCTTC 2 cut(s) 19, 96
Eco147I AGGCCT 1 cut(s) 172
EcoRI GAATTC 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 284
FaeI CATG 3 cut(s) 249, 286, 404
FatI CATG 3 cut(s) 245, 282, 400
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 4 cut(s) 102, 186, 344, 447
FokI GGATG 3 cut(s) 77, 149, 269
Fsp4HI GCNGC 4 cut(s) 102, 186, 344, 447
FspBI CTAG 1 cut(s) 201
GluI GCNGC 4 cut(s) 102, 186, 344, 447
GsaI CCCAGC 1 cut(s) 481
HaeIII GGCC 2 cut(s) 172, 297
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 3 cut(s) 249, 286, 404
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 1 cut(s) 197
HphI GGTGA 2 cut(s) 118, 454
Hpy166II GTNNAC 2 cut(s) 555, 570
Hpy188I TCNGA 3 cut(s) 114, 226, 337
Hpy188III TCNNGA 4 cut(s) 134, 201, 268, 487
Hpy8I GTNNAC 2 cut(s) 555, 570
Hpy99I CGWCG 2 cut(s) 24, 27
HpyAV CCTTC 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 104, 188, 282
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 443
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 3 cut(s) 249, 286, 404
Kzo9I GATC 2 cut(s) 203, 328
LpnPI CCDG 4 cut(s) 43, 186, 459, 463
Lsp1109I GCAGC 3 cut(s) 88, 172, 433
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 460
MalI GATC 2 cut(s) 205, 330
MboI GATC 2 cut(s) 203, 328
MboII GAAGA 7 cut(s) 6, 83, 231, 307, 416, 430, 552
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 2 cut(s) 203, 328
MluCI AATT 5 cut(s) 120, 189, 490, 513, 522
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 4 cut(s) 22, 97, 187, 317
Mph1103I ATGCAT 1 cut(s) 284
MunI CAATTG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 352, 443
NdeII GATC 2 cut(s) 203, 328
NlaIII CATG 3 cut(s) 249, 286, 404
NlaIV GGNNCC 3 cut(s) 330, 347, 378
NmuCI GTSAC 1 cut(s) 460
NsiI ATGCAT 1 cut(s) 284
PceI AGGCCT 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PkrI GCNGC 4 cut(s) 103, 187, 345, 448
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 330, 347, 378
PspPI GGNCC 1 cut(s) 295
PsuI RGATCY 2 cut(s) 203, 328
SatI GCNGC 4 cut(s) 102, 186, 344, 447
Sau3AI GATC 2 cut(s) 203, 328
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 1 cut(s) 206
SetI ASST 6 cut(s) 14, 131, 187, 369, 448, 462
SfaNI GCATC 1 cut(s) 14
Sse9I AATT 5 cut(s) 120, 189, 490, 513, 522
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 1 cut(s) 343
SspMI CTAG 1 cut(s) 201
StuI AGGCCT 1 cut(s) 172
TaiI ACGT 1 cut(s) 462
TaqII GACCGA 1 cut(s) 156
TasI AATT 5 cut(s) 120, 189, 490, 513, 522
TauI GCSGC 1 cut(s) 346
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 3 cut(s) 101, 185, 446
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 4 cut(s) 273, 299, 417, 570
TspGWI ACGGA 1 cut(s) 538
XapI RAATTY 1 cut(s) 120
XbaI TCTAGA 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 1 cut(s) 201
ZraI GACGTC 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.