FvH4_3g35831

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000466
Physical Location & Seq
Forward (+)
30762385 .. 30762897
513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35831.t1

Sequence Viewer

Length: 513 bp
ATGGCTCTGAGCAGTCCAAGAGCCTCTGCATATATTCCTTCATCAGCTGATCCTCAGTTCAAGCATGATTTCTTTCTGAGTTTCAGGGGTGAGGATACTCGCAAGGGGATTACATCCCAATTGTATGAGGAATTGCAACACAGGAGAGGAATAAAAACATTCAAGGATGACAAAGAGCTCGAAGTAGGGGTGTCTATTGCTCCTAGTCTCCTAATGGCAATCGAAGAATCCAGGTTCGCTATCGTTGTTCTGTCGCCAAACTATGCTTCTTCTCCATGGTGTCTGGATGAACTTACAAAGATTTGTCAATGCATGGATGGCAAGGACTCAATTCTGCCTCTATTTTATCATGTGGATCCATCTGATGTACGACACCAAAAGAATAGTTTTGAAGTGGCCTTCGCTAAGCATGAAGAAAAATTTAGGCAACCGAAGGACAGGGTGAAGCAGTGGAGAGTTGCTTTACAGAAAGTGGCCAATGTATCCGGGTGGGATTCAAAGAATTACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

171

Amino Acids

19.46

Weight (kDa)

7.73

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 168 1.4e-47 TIR domain
TIR_2 PF13676 24 - 125 6.2e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 44, 350, 363
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 419
AfaI GTAC 1 cut(s) 369
AgsI TTSAA 4 cut(s) 61, 163, 392, 498
AjnI CCWGG 1 cut(s) 230
AjuI GAANNNNNNNTTGG 2 cut(s) 250, 282
AluBI AGCT 2 cut(s) 47, 178
AluI AGCT 2 cut(s) 47, 178
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 3 cut(s) 44, 350, 363
AoxI GGCC 2 cut(s) 396, 474
ApoI RAATTY 1 cut(s) 419
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 101, 454
BalI TGGCCA 1 cut(s) 476
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BccI CCATC 2 cut(s) 311, 367
BciT130I CCWGG 1 cut(s) 232
BciVI GTATCC 2 cut(s) 88, 493
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 1 cut(s) 204
BfuI GTATCC 2 cut(s) 88, 493
BlpI GCTNAGC 1 cut(s) 405
Bme1390I CCNGG 2 cut(s) 232, 487
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 2 cut(s) 232, 487
Bpu1102I GCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 4 cut(s) 113, 172, 292, 322
BseMII CTCAG 2 cut(s) 68, 68
BshFI GGCC 2 cut(s) 398, 476
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 1 cut(s) 486
BsmAI GTCTC 1 cut(s) 212
BsnI GGCC 2 cut(s) 398, 476
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 49, 355
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 1 cut(s) 275
BspANI GGCC 2 cut(s) 398, 476
BspCNI CTCAG 2 cut(s) 67, 69
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 3 cut(s) 44, 350, 363
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 2 cut(s) 49, 355
BssT1I CCWWGG 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 232
BstDEI CTNAG 4 cut(s) 8, 54, 77, 405
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 4 cut(s) 113, 172, 292, 322
BstKTI GATC 2 cut(s) 52, 358
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 49, 355
BstMWI GCNNNNNNNGC 1 cut(s) 318
BstNI CCWGG 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 230, 485
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuI GTATCC 2 cut(s) 88, 493
BsuRI GGCC 2 cut(s) 398, 476
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 4 cut(s) 113, 172, 292, 322
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
Csp6I GTAC 1 cut(s) 368
CviAII CATG 5 cut(s) 65, 276, 313, 350, 410
CviJI RGCY 6 cut(s) 5, 23, 47, 178, 398, 476
CviKI_1 RGCY 6 cut(s) 5, 23, 47, 178, 398, 476
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 4 cut(s) 8, 54, 77, 405
DpnI GATC 2 cut(s) 51, 357
DpnII GATC 2 cut(s) 49, 355
EaeI YGGCCR 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 180
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 5 cut(s) 68, 279, 316, 353, 413
FaiI YATR 9 cut(s) 31, 33, 66, 126, 264, 277, 314, 351, 411
FatI CATG 5 cut(s) 64, 275, 312, 349, 409
FokI GGATG 4 cut(s) 100, 179, 299, 329
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 1 cut(s) 204
HaeIII GGCC 2 cut(s) 398, 476
HapII CCGG 1 cut(s) 486
Hin1II CATG 5 cut(s) 68, 279, 316, 353, 413
HinfI GANTC 3 cut(s) 227, 326, 494
HpaII CCGG 1 cut(s) 486
HphI GGTGA 2 cut(s) 101, 454
Hpy188I TCNGA 3 cut(s) 9, 78, 364
Hpy188III TCNNGA 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 48, 409, 427
HpyCH4V TGCA 3 cut(s) 29, 136, 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 318
HpyF3I CTNAG 4 cut(s) 8, 54, 77, 405
Hsp92II CATG 5 cut(s) 68, 279, 316, 353, 413
Kzo9I GATC 2 cut(s) 49, 355
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 7 cut(s) 70, 127, 217, 244, 269, 424, 499
MaeI CTAG 1 cut(s) 204
MalI GATC 2 cut(s) 51, 357
MboI GATC 2 cut(s) 49, 355
MboII GAAGA 3 cut(s) 236, 261, 425
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 5 cut(s) 119, 131, 330, 419, 502
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 6 cut(s) 34, 63, 85, 121, 140, 348
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 1 cut(s) 314
MscI TGGCCA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 2 cut(s) 232, 487
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 318
NciI CCSGG 1 cut(s) 487
NcoI CCATGG 1 cut(s) 275
NdeII GATC 2 cut(s) 49, 355
NlaIII CATG 5 cut(s) 68, 279, 316, 353, 413
NlaIV GGNNCC 1 cut(s) 357
NsiI ATGCAT 1 cut(s) 314
PfeI GAWTC 2 cut(s) 227, 494
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 357
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SacI GAGCTC 1 cut(s) 180
Sau3AI GATC 2 cut(s) 49, 355
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 232, 487
SduI GDGCHC 1 cut(s) 180
SetI ASST 3 cut(s) 49, 180, 236
Sse9I AATT 5 cut(s) 119, 131, 330, 419, 502
SspMI CTAG 1 cut(s) 204
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 230, 485
StyI CCWWGG 1 cut(s) 275
TaqI TCGA 2 cut(s) 180, 222
TasI AATT 5 cut(s) 119, 131, 330, 419, 502
TfiI GAWTC 2 cut(s) 227, 494
TscAI CASTG 1 cut(s) 455
TspDTI ATGAA 3 cut(s) 30, 303, 426
TspRI CASTG 1 cut(s) 455
XapI RAATTY 1 cut(s) 419
XspI CTAG 1 cut(s) 204
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.