Mitogen-activated protein kinase kinase kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 351 |
| Acc65I | GGTACC | 1 cut(s) | 375 |
| AccB1I | GGYRCC | 1 cut(s) | 375 |
| AccBSI | CCGCTC | 1 cut(s) | 209 |
| AciI | CCGC | 2 cut(s) | 207, 451 |
| AclWI | GGATC | 1 cut(s) | 167 |
| AcoI | YGGCCR | 1 cut(s) | 338 |
| AcsI | RAATTY | 2 cut(s) | 333, 505 |
| AcuI | CTGAAG | 1 cut(s) | 167 |
| AfaI | GTAC | 2 cut(s) | 372, 377 |
| AgsI | TTSAA | 2 cut(s) | 32, 227 |
| AluBI | AGCT | 3 cut(s) | 56, 401, 420 |
| AluI | AGCT | 3 cut(s) | 56, 401, 420 |
| AlwI | GGATC | 1 cut(s) | 167 |
| AoxI | GGCC | 2 cut(s) | 3, 338 |
| ApeKI | GCWGC | 1 cut(s) | 448 |
| ApoI | RAATTY | 2 cut(s) | 333, 505 |
| Asp718I | GGTACC | 1 cut(s) | 375 |
| AspS9I | GGNCC | 1 cut(s) | 95 |
| AvaII | GGWCC | 1 cut(s) | 95 |
| BalI | TGGCCA | 1 cut(s) | 340 |
| BanI | GGYRCC | 1 cut(s) | 375 |
| BbsI | GAAGAC | 1 cut(s) | 327 |
| BbvI | GCAGC | 1 cut(s) | 460 |
| BccI | CCATC | 1 cut(s) | 141 |
| BfaI | CTAG | 1 cut(s) | 423 |
| BfmI | CTRYAG | 1 cut(s) | 140 |
| BglII | AGATCT | 2 cut(s) | 178, 303 |
| BisI | GCNGC | 1 cut(s) | 449 |
| BlsI | GCNGC | 1 cut(s) | 450 |
| Bme18I | GGWCC | 1 cut(s) | 95 |
| BmgT120I | GGNCC | 1 cut(s) | 95 |
| BmiI | GGNNCC | 3 cut(s) | 96, 360, 377 |
| BmsI | GCATC | 3 cut(s) | 20, 123, 451 |
| BpiI | GAAGAC | 1 cut(s) | 327 |
| BpuEI | CTTGAG | 1 cut(s) | 103 |
| BsaBI | GATNNNNATC | 1 cut(s) | 177 |
| BsaJI | CCNNGG | 1 cut(s) | 62 |
| BsaWI | WCCGGW | 1 cut(s) | 378 |
| Bse1I | ACTGG | 1 cut(s) | 480 |
| Bse8I | GATNNNNATC | 1 cut(s) | 177 |
| BseDI | CCNNGG | 1 cut(s) | 62 |
| BseGI | GGATG | 3 cut(s) | 72, 175, 199 |
| BseJI | GATNNNNATC | 1 cut(s) | 177 |
| BseNI | ACTGG | 1 cut(s) | 480 |
| BseXI | GCAGC | 1 cut(s) | 460 |
| BsgI | GTGCAG | 1 cut(s) | 467 |
| BshFI | GGCC | 2 cut(s) | 5, 340 |
| BshNI | GGYRCC | 1 cut(s) | 375 |
| BsiSI | CCGG | 1 cut(s) | 379 |
| BsnI | GGCC | 2 cut(s) | 5, 340 |
| Bsp143I | GATC | 4 cut(s) | 124, 172, 178, 303 |
| BspACI | CCGC | 2 cut(s) | 207, 451 |
| BspANI | GGCC | 2 cut(s) | 5, 340 |
| BspLI | GGNNCC | 3 cut(s) | 96, 360, 377 |
| BspMAI | CTGCAG | 1 cut(s) | 144 |
| BspPI | GGATC | 1 cut(s) | 167 |
| BspQI | GCTCTTC | 1 cut(s) | 377 |
| BspT107I | GGYRCC | 1 cut(s) | 375 |
| BsrBI | CCGCTC | 1 cut(s) | 209 |
| BsrI | ACTGG | 1 cut(s) | 480 |
| BssECI | CCNNGG | 1 cut(s) | 62 |
| BssMI | GATC | 4 cut(s) | 124, 172, 178, 303 |
| Bst4CI | ACNGT | 1 cut(s) | 375 |
| Bst6I | CTCTTC | 3 cut(s) | 21, 140, 377 |
| BstDSI | CCRYGG | 1 cut(s) | 62 |
| BstF5I | GGATG | 3 cut(s) | 72, 175, 199 |
| BstKTI | GATC | 4 cut(s) | 127, 175, 181, 306 |
| BstMBI | GATC | 4 cut(s) | 124, 172, 178, 303 |
| BstSFI | CTRYAG | 1 cut(s) | 140 |
| BstV1I | GCAGC | 1 cut(s) | 460 |
| BstV2I | GAAGAC | 1 cut(s) | 327 |
| BstX2I | RGATCY | 2 cut(s) | 178, 303 |
| BstYI | RGATCY | 2 cut(s) | 178, 303 |
| BsuRI | GGCC | 2 cut(s) | 5, 340 |
| BtgI | CCRYGG | 1 cut(s) | 62 |
| BtsCI | GGATG | 3 cut(s) | 72, 175, 199 |
| Cfr13I | GGNCC | 1 cut(s) | 95 |
| Csp6I | GTAC | 2 cut(s) | 371, 376 |
| CviAII | CATG | 1 cut(s) | 166 |
| CviJI | RGCY | 6 cut(s) | 5, 56, 340, 401, 420, 479 |
| CviKI_1 | RGCY | 6 cut(s) | 5, 56, 340, 401, 420, 479 |
| CviQI | GTAC | 2 cut(s) | 371, 376 |
| DpnI | GATC | 4 cut(s) | 126, 174, 180, 305 |
| DpnII | GATC | 4 cut(s) | 124, 172, 178, 303 |
| DraI | TTTAAA | 1 cut(s) | 468 |
| EaeI | YGGCCR | 1 cut(s) | 338 |
| Eam1104I | CTCTTC | 3 cut(s) | 21, 140, 377 |
| EarI | CTCTTC | 3 cut(s) | 21, 140, 377 |
| Eco47I | GGWCC | 1 cut(s) | 95 |
| Eco57I | CTGAAG | 1 cut(s) | 167 |
| EcoO109I | RGGNCCY | 1 cut(s) | 95 |
| FaeI | CATG | 1 cut(s) | 169 |
| FaiI | YATR | 8 cut(s) | 72, 130, 167, 267, 317, 351, 414, 498 |
| FatI | CATG | 1 cut(s) | 165 |
| FauI | CCCGC | 1 cut(s) | 214 |
| Fnu4HI | GCNGC | 1 cut(s) | 449 |
| FokI | GGATG | 3 cut(s) | 79, 182, 206 |
| Fsp4HI | GCNGC | 1 cut(s) | 449 |
| FspBI | CTAG | 1 cut(s) | 423 |
| GluI | GCNGC | 1 cut(s) | 449 |
| HaeIII | GGCC | 2 cut(s) | 5, 340 |
| HapII | CCGG | 1 cut(s) | 379 |
| Hin1II | CATG | 1 cut(s) | 169 |
| HindIII | AAGCTT | 1 cut(s) | 399 |
| HinfI | GANTC | 1 cut(s) | 32 |
| HpaII | CCGG | 1 cut(s) | 379 |
| Hpy188I | TCNGA | 1 cut(s) | 124 |
| Hpy188III | TCNNGA | 3 cut(s) | 82, 98, 176 |
| HpyAV | CCTTC | 2 cut(s) | 30, 102 |
| HpyCH4III | ACNGT | 1 cut(s) | 375 |
| HpyCH4V | TGCA | 5 cut(s) | 11, 142, 221, 242, 448 |
| Hsp92II | CATG | 1 cut(s) | 169 |
| KpnI | GGTACC | 1 cut(s) | 379 |
| Kzo9I | GATC | 4 cut(s) | 124, 172, 178, 303 |
| LguI | GCTCTTC | 1 cut(s) | 377 |
| LmnI | GCTCC | 1 cut(s) | 111 |
| LpnPI | CCDG | 7 cut(s) | 76, 111, 170, 189, 392, 474, 493 |
| Lsp1109I | GCAGC | 1 cut(s) | 460 |
| LweI | GCATC | 3 cut(s) | 20, 123, 451 |
| MaeI | CTAG | 1 cut(s) | 423 |
| MalI | GATC | 4 cut(s) | 126, 174, 180, 305 |
| MbiI | CCGCTC | 1 cut(s) | 209 |
| MboI | GATC | 4 cut(s) | 124, 172, 178, 303 |
| MboII | GAAGA | 5 cut(s) | 8, 157, 173, 332, 394 |
| MfeI | CAATTG | 2 cut(s) | 222, 243 |
| MflI | RGATCY | 2 cut(s) | 178, 303 |
| MlsI | TGGCCA | 1 cut(s) | 340 |
| MluCI | AATT | 5 cut(s) | 153, 222, 243, 333, 505 |
| MluNI | TGGCCA | 1 cut(s) | 340 |
| MmeI | TCCRAC | 1 cut(s) | 237 |
| MnlI | CCTC | 4 cut(s) | 16, 141, 283, 373 |
| Mox20I | TGGCCA | 1 cut(s) | 340 |
| MscI | TGGCCA | 1 cut(s) | 340 |
| MseI | TTAA | 3 cut(s) | 156, 467, 510 |
| Msp20I | TGGCCA | 1 cut(s) | 340 |
| MspA1I | CMGCKG | 1 cut(s) | 451 |
| MspI | CCGG | 1 cut(s) | 379 |
| MunI | CAATTG | 2 cut(s) | 222, 243 |
| NdeII | GATC | 4 cut(s) | 124, 172, 178, 303 |
| NlaIII | CATG | 1 cut(s) | 169 |
| NlaIV | GGNNCC | 3 cut(s) | 96, 360, 377 |
| PciSI | GCTCTTC | 1 cut(s) | 377 |
| PfeI | GAWTC | 1 cut(s) | 32 |
| PkrI | GCNGC | 1 cut(s) | 450 |
| PpuMI | RGGWCCY | 1 cut(s) | 95 |
| PsiI | TTATAA | 1 cut(s) | 351 |
| Psp5II | RGGWCCY | 1 cut(s) | 95 |
| PspN4I | GGNNCC | 3 cut(s) | 96, 360, 377 |
| PspPI | GGNCC | 1 cut(s) | 95 |
| PspPPI | RGGWCCY | 1 cut(s) | 95 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 62, 94 |
| PstI | CTGCAG | 1 cut(s) | 144 |
| PsuI | RGATCY | 2 cut(s) | 178, 303 |
| RsaI | GTAC | 2 cut(s) | 372, 377 |
| RsaNI | GTAC | 2 cut(s) | 371, 376 |
| SapI | GCTCTTC | 1 cut(s) | 377 |
| SaqAI | TTAA | 3 cut(s) | 156, 467, 510 |
| SatI | GCNGC | 1 cut(s) | 449 |
| Sau3AI | GATC | 4 cut(s) | 124, 172, 178, 303 |
| Sau96I | GGNCC | 1 cut(s) | 95 |
| SetI | ASST | 5 cut(s) | 58, 189, 239, 403, 422 |
| SfaNI | GCATC | 3 cut(s) | 20, 123, 451 |
| SfcI | CTRYAG | 1 cut(s) | 140 |
| SinI | GGWCC | 1 cut(s) | 95 |
| SmlI | CTYRAG | 1 cut(s) | 82 |
| SmoI | CTYRAG | 1 cut(s) | 82 |
| Sse9I | AATT | 5 cut(s) | 153, 222, 243, 333, 505 |
| SsiI | CCGC | 2 cut(s) | 207, 451 |
| SspMI | CTAG | 1 cut(s) | 423 |
| TaaI | ACNGT | 1 cut(s) | 375 |
| TaqI | TCGA | 3 cut(s) | 106, 136, 405 |
| TasI | AATT | 5 cut(s) | 153, 222, 243, 333, 505 |
| TfiI | GAWTC | 1 cut(s) | 32 |
| Tru1I | TTAA | 3 cut(s) | 156, 467, 510 |
| Tru9I | TTAA | 3 cut(s) | 156, 467, 510 |
| TseI | GCWGC | 1 cut(s) | 448 |
| TspDTI | ATGAA | 2 cut(s) | 12, 154 |
| TspGWI | ACGGA | 1 cut(s) | 51 |
| VpaK11BI | GGWCC | 1 cut(s) | 95 |
| XapI | RAATTY | 2 cut(s) | 333, 505 |
| XspI | CTAG | 1 cut(s) | 423 |