RLG00000030347

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000466
Physical Location & Seq
Forward (+)
59883205 .. 59885215
2011 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030347

Sequence Viewer

Length: 1035 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTCAATTTCAGGGGGGAAGATACCCGCAATAGTTTTGCCGGTCCTCTGTATGATGAACTAAATCGGAAAGGAATTCTCACCTTTATGGATGAGCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTAGCTGGGCCAACAAGTCCTCCCCGTCTTCTTCTGCGGCGCGGATCCTTCTGATGTGCTGCACAAAGCTCAAATGGGAACACCAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAACGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACACAAGTCGCCAATCTTTCTGGCTGGGATTTCAACCATTATCCACTCAAACCATCAAAAGTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCATTGATGATTTATTAAGCAATTACATATATCCGCAGCTTGGTGGGGTGCGTTTTATTGGGATACATGGCATGGGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCAATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGCTTAAACTTTGATGAAGCGCTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAACATTATTTAGATCTGTCCAATAGCCACGGATTTGGAGGGGGTCTGGATCAAAATCCGACCAAACCCGTGCTGTGCAGCAAAATTGAAGCCAAACAACCAGAGCATTCCCGTGACTACACGACCATGGAGGACCTCAGCATAACTGGCAAATCTCCCTCCTCTATCGACAATCCCCTCACGCCCGACCATGCATGCACCATCATCTCGCGAACCACATCATACCAAGGCACCAAAGCTCTTATTCTCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

345

Amino Acids

38.43

Weight (kDa)

6.56

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 70 7.7e-15 TIR domain
NB-ARC PF00931 187 - 221 2.2e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 1010
AccI GTMKAC 1 cut(s) 470
AccII CGCG 2 cut(s) 250, 991
AciI CCGC 5 cut(s) 70, 245, 250, 514, 548
AclWI GGATC 3 cut(s) 247, 260, 837
AcsI RAATTY 2 cut(s) 117, 633
AcyI GRCGYC 1 cut(s) 5
AfeI AGCGCT 1 cut(s) 735
AfiI CCNNNNNNNGG 1 cut(s) 554
AgsI TTSAA 5 cut(s) 400, 480, 740, 754, 869
AluBI AGCT 8 cut(s) 145, 182, 213, 277, 359, 553, 620, 1019
AluI AGCT 8 cut(s) 145, 182, 213, 277, 359, 553, 620, 1019
AlwI GGATC 3 cut(s) 247, 260, 837
Ama87I CYCGRG 1 cut(s) 615
Aor51HI AGCGCT 1 cut(s) 735
AoxI GGCC 2 cut(s) 167, 216
ApeKI GCWGC 5 cut(s) 182, 267, 359, 550, 858
ApoI RAATTY 2 cut(s) 117, 633
Asp700I GAANNNNTTC 2 cut(s) 508, 735
AspLEI GCGC 2 cut(s) 250, 736
AspS9I GGNCC 3 cut(s) 86, 216, 913
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 2 cut(s) 86, 913
BamHI GGATCC 1 cut(s) 252
BanI GGYRCC 1 cut(s) 1010
BbsI GAAGAC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 917
BbvI GCAGC 5 cut(s) 169, 254, 346, 562, 870
BccI CCATC 2 cut(s) 427, 989
BceAI ACGGC 1 cut(s) 730
BciVI GTATCC 1 cut(s) 570
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 737
BfuAI ACCTGC 1 cut(s) 665
BfuI GTATCC 1 cut(s) 570
BglII AGATCT 2 cut(s) 200, 793
BisI GCNGC 6 cut(s) 183, 246, 268, 360, 551, 859
BlsI GCNGC 6 cut(s) 184, 247, 269, 361, 552, 860
Bme18I GGWCC 2 cut(s) 86, 913
BmeT110I CYCGRG 1 cut(s) 615
BmgT120I GGNCC 3 cut(s) 86, 216, 913
BmiI GGNNCC 3 cut(s) 254, 511, 1012
BmsI GCATC 1 cut(s) 685
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 917
BpuEI CTTGAG 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 808, 906, 1006
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 834
BseDI CCNNGG 3 cut(s) 808, 906, 1006
BseGI GGATG 1 cut(s) 139
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMII CTCAG 1 cut(s) 931
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 2 cut(s) 206, 931
BseXI GCAGC 5 cut(s) 169, 254, 346, 562, 870
BseYI CCCAGC 2 cut(s) 213, 390
BsgI GTGCAG 2 cut(s) 253, 877
Bsh1236I CGCG 2 cut(s) 250, 991
BshFI GGCC 2 cut(s) 169, 218
BshNI GGYRCC 1 cut(s) 1010
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 1 cut(s) 554
BsmFI GGGAC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 2 cut(s) 169, 218
BsoBI CYCGRG 1 cut(s) 615
Bsp143I GATC 4 cut(s) 200, 252, 793, 829
Bsp19I CCATGG 1 cut(s) 906
Bsp68I TCGCGA 1 cut(s) 991
BspACI CCGC 5 cut(s) 70, 245, 250, 514, 548
BspANI GGCC 2 cut(s) 169, 218
BspCNI CTCAG 1 cut(s) 930
BspFNI CGCG 2 cut(s) 250, 991
BspLI GGNNCC 3 cut(s) 254, 511, 1012
BspMI ACCTGC 1 cut(s) 665
BspPI GGATC 3 cut(s) 247, 260, 837
BspT107I GGYRCC 1 cut(s) 1010
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 1 cut(s) 931
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 808, 906, 1006
BssMI GATC 4 cut(s) 200, 252, 793, 829
BssNAI GTATAC 1 cut(s) 471
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 906, 1006
Bst1107I GTATAC 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 976
BstDEI CTNAG 1 cut(s) 917
BstDSI CCRYGG 2 cut(s) 808, 906
BstF5I GGATG 1 cut(s) 139
BstFNI CGCG 2 cut(s) 250, 991
BstH2I RGCGCY 1 cut(s) 737
BstHHI GCGC 2 cut(s) 250, 736
BstKTI GATC 4 cut(s) 203, 255, 796, 832
BstMBI GATC 4 cut(s) 200, 252, 793, 829
BstMWI GCNNNNNNNGC 3 cut(s) 356, 591, 927
BstNSI RCATGY 1 cut(s) 978
BstPAI GACNNNNGTC 1 cut(s) 371
BstUI CGCG 2 cut(s) 250, 991
BstV1I GCAGC 5 cut(s) 169, 254, 346, 562, 870
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 3 cut(s) 200, 252, 793
BstXI CCANNNNNNTGG 1 cut(s) 815
BstYI RGATCY 3 cut(s) 200, 252, 793
BstZ17I GTATAC 1 cut(s) 471
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 2 cut(s) 169, 218
BtgI CCRYGG 2 cut(s) 808, 906
BtsCI GGATG 1 cut(s) 139
BtuMI TCGCGA 1 cut(s) 991
BveI ACCTGC 1 cut(s) 665
Cac8I GCNNGC 1 cut(s) 976
CfoI GCGC 2 cut(s) 250, 736
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 86, 216, 913
CviAII CATG 7 cut(s) 311, 581, 586, 686, 907, 971, 975
DdeI CTNAG 1 cut(s) 917
DpnI GATC 4 cut(s) 202, 254, 795, 831
DpnII GATC 4 cut(s) 200, 252, 793, 829
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 906, 1006
Eco147I AGGCCT 1 cut(s) 169
Eco47I GGWCC 2 cut(s) 86, 913
Eco47III AGCGCT 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 117
EcoT14I CCWWGG 2 cut(s) 906, 1006
EcoT22I ATGCAT 1 cut(s) 976
ErhI CCWWGG 2 cut(s) 906, 1006
FaeI CATG 7 cut(s) 314, 584, 589, 689, 910, 974, 978
FaqI GGGAC 1 cut(s) 756
FatI CATG 7 cut(s) 310, 580, 585, 685, 906, 970, 974
FauI CCCGC 2 cut(s) 77, 521
FblI GTMKAC 1 cut(s) 470
Fnu4HI GCNGC 6 cut(s) 183, 246, 268, 360, 551, 859
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 6 cut(s) 183, 246, 268, 360, 551, 859
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 249, 735
GluI GCNGC 6 cut(s) 183, 246, 268, 360, 551, 859
GsaI CCCAGC 2 cut(s) 217, 394
HaeII RGCGCY 1 cut(s) 737
HaeIII GGCC 2 cut(s) 169, 218
HapII CCGG 1 cut(s) 84
HhaI GCGC 2 cut(s) 250, 736
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 314, 584, 589, 689, 910, 974, 978
Hin6I GCGC 2 cut(s) 248, 734
HinP1I GCGC 2 cut(s) 248, 734
HincII GTYRAC 2 cut(s) 9, 487
HindII GTYRAC 2 cut(s) 9, 487
HinfI GANTC 1 cut(s) 194
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 3 cut(s) 9, 471, 487
Hpy188I TCNGA 3 cut(s) 111, 261, 840
Hpy188III TCNNGA 4 cut(s) 198, 641, 827, 990
Hpy8I GTNNAC 3 cut(s) 9, 471, 487
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 266, 748
HpyCH4V TGCA 5 cut(s) 185, 270, 858, 974, 978
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 591, 927
HpyF3I CTNAG 1 cut(s) 917
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 314, 584, 589, 689, 910, 974, 978
HspAI GCGC 2 cut(s) 248, 734
Kzo9I GATC 4 cut(s) 200, 252, 793, 829
Lsp1109I GCAGC 5 cut(s) 169, 254, 346, 562, 870
LweI GCATC 1 cut(s) 685
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 893
MalI GATC 4 cut(s) 202, 254, 795, 831
MboI GATC 4 cut(s) 200, 252, 793, 829
MboII GAAGA 7 cut(s) 6, 74, 228, 231, 326, 340, 468
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 3 cut(s) 200, 252, 793
MluCI AATT 9 cut(s) 49, 117, 186, 429, 438, 535, 633, 670, 864
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 2 cut(s) 508, 735
MseI TTAA 5 cut(s) 444, 530, 677, 719, 762
MslI CAYNNNNRTG 4 cut(s) 128, 503, 891, 905
MspI CCGG 1 cut(s) 84
MunI CAATTG 1 cut(s) 186
Mva1269I GAATGC 1 cut(s) 886
MvnI CGCG 2 cut(s) 250, 991
MwoI GCNNNNNNNGC 3 cut(s) 356, 591, 927
NcoI CCATGG 1 cut(s) 906
NdeII GATC 4 cut(s) 200, 252, 793, 829
NlaIII CATG 7 cut(s) 314, 584, 589, 689, 910, 974, 978
NlaIV GGNNCC 3 cut(s) 254, 511, 1012
NmuCI GTSAC 1 cut(s) 893
NruI TCGCGA 1 cut(s) 991
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 1 cut(s) 978
PaeI GCATGC 1 cut(s) 978
PaeR7I CTCGAG 1 cut(s) 615
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 2 cut(s) 508, 735
PkrI GCNGC 6 cut(s) 184, 247, 269, 361, 552, 860
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 913
PshAI GACNNNNGTC 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 913
PspFI CCCAGC 2 cut(s) 213, 390
PspN4I GGNNCC 3 cut(s) 254, 511, 1012
PspPI GGNCC 3 cut(s) 86, 216, 913
PspPPI RGGWCCY 1 cut(s) 913
PspXI VCTCGAGB 1 cut(s) 615
PsuI RGATCY 3 cut(s) 200, 252, 793
RruI TCGCGA 1 cut(s) 991
RseI CAYNNNNRTG 4 cut(s) 128, 503, 891, 905
SaqAI TTAA 5 cut(s) 444, 530, 677, 719, 762
SatI GCNGC 6 cut(s) 183, 246, 268, 360, 551, 859
Sau3AI GATC 4 cut(s) 200, 252, 793, 829
Sau96I GGNCC 3 cut(s) 86, 216, 913
SchI GAGTC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 685
Sfr274I CTCGAG 1 cut(s) 615
SinI GGWCC 2 cut(s) 86, 913
SlaI CTCGAG 1 cut(s) 615
SmiMI CAYNNNNRTG 4 cut(s) 128, 503, 891, 905
SmlI CTYRAG 2 cut(s) 146, 615
SmoI CTYRAG 2 cut(s) 146, 615
SphI GCATGC 1 cut(s) 978
Sse9I AATT 9 cut(s) 49, 117, 186, 429, 438, 535, 633, 670, 864
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 5 cut(s) 70, 245, 250, 514, 548
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 169
StyI CCWWGG 2 cut(s) 906, 1006
TaqI TCGA 2 cut(s) 616, 948
TaqII GACCGA 1 cut(s) 666
TasI AATT 9 cut(s) 49, 117, 186, 429, 438, 535, 633, 670, 864
TauI GCSGC 1 cut(s) 248
Tru1I TTAA 5 cut(s) 444, 530, 677, 719, 762
Tru9I TTAA 5 cut(s) 444, 530, 677, 719, 762
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 5 cut(s) 182, 267, 359, 550, 858
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 5 cut(s) 114, 327, 487, 645, 744
TspGWI ACGGA 2 cut(s) 454, 825
VpaK11BI GGWCC 2 cut(s) 86, 913
XapI RAATTY 2 cut(s) 117, 633
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 978
XhoI CTCGAG 1 cut(s) 615
XmiI GTMKAC 1 cut(s) 470
XmnI GAANNNNTTC 2 cut(s) 508, 735
XspI CTAG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.