FvH4_7g08891

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000466
Physical Location & Seq
Forward (+)
8690113 .. 8690911
799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08891.t1

Sequence Viewer

Length: 498 bp
ATGGCCTTTGCACCTTTACCATCAGCTCCTGTGTGGAAGTATGATATGTTTTTGAGTTTTAGCGGGAAAGACACTCGCAAGGGTATCGTGTCAACTCTCTACCATGAATTGAAAAAACTCAGTAACTTCAACCCTTTCATGGATAGCCAAGAGCTCAAACCGGGTGCCGAAATTTCCTCGGATCTCCTAACAGCAATCCAGGAATCGAAATCTGTGATAATTTTTCTCTCGCCAAACTATGATTCTTCCGCATGGTGTTTGGATGAACTTGAAATCATTGTTCAGTGCTTGGAAACCAGGAACACAAAAGTTATCCCACTTTTCTATAATGTAGATCCATCTGATGTGCGACATCAACTCCGGACTTTTGCAGAAGCCTTCACTAGGCATGAACACAGGTATTCCCAAGAGGATGGCATACAGAAGGTGAAACGGTGGAGAACTGCTTTAACAAAAGTGGCTAATCTCTCTGGCTTGGAATCAAGCAAATTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

166

Amino Acids

18.91

Weight (kDa)

6.43

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 164 8.3e-45 TIR domain
TIR_2 PF13676 17 - 126 2.1e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 360
AciI CCGC 2 cut(s) 63, 249
AclWI GGATC 2 cut(s) 189, 329
AcsI RAATTY 2 cut(s) 171, 488
AfiI CCNNNNNNNGG 2 cut(s) 139, 384
AgsI TTSAA 3 cut(s) 112, 130, 272
AjnI CCWGG 2 cut(s) 198, 296
AjuI GAANNNNNNNTTGG 2 cut(s) 226, 258
AluBI AGCT 2 cut(s) 26, 154
AluI AGCT 2 cut(s) 26, 154
Alw21I GWGCWC 1 cut(s) 156
AlwI GGATC 2 cut(s) 189, 329
AlwNI CAGNNNCTG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 360
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 2 cut(s) 171, 488
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 439
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 156
BccI CCATC 3 cut(s) 28, 346, 407
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 200, 298
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 1 cut(s) 384
Bme1390I CCNGG 3 cut(s) 162, 200, 298
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 162, 200, 298
BpuMI CCSGG 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 1 cut(s) 360
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 384
BseAI TCCGGA 1 cut(s) 360
BseBI CCWGG 2 cut(s) 200, 298
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 2 cut(s) 268, 418
BseLI CCNNNNNNNGG 2 cut(s) 139, 384
BseMII CTCAG 1 cut(s) 133
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 2 cut(s) 161, 361
BslI CCNNNNNNNGG 2 cut(s) 139, 384
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 360
Bsp143I GATC 2 cut(s) 181, 334
BspACI CCGC 2 cut(s) 63, 249
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 132
BspEI TCCGGA 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 2 cut(s) 189, 329
BspT107I GGYRCC 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 2 cut(s) 181, 334
Bst2UI CCWGG 2 cut(s) 200, 298
Bst4CI ACNGT 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 119
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 2 cut(s) 268, 418
BstKTI GATC 2 cut(s) 184, 337
BstMBI GATC 2 cut(s) 181, 334
BstNI CCWGG 2 cut(s) 200, 298
BstSCI CCNGG 3 cut(s) 160, 198, 296
BstX2I RGATCY 2 cut(s) 181, 334
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 2 cut(s) 181, 334
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 268, 418
BtsIMutI CAGTG 1 cut(s) 290
CaiI CAGNNNCTG 1 cut(s) 29
CviAII CATG 4 cut(s) 104, 139, 252, 389
CviJI RGCY 7 cut(s) 5, 26, 147, 154, 377, 461, 474
CviKI_1 RGCY 7 cut(s) 5, 26, 147, 154, 377, 461, 474
DdeI CTNAG 1 cut(s) 119
DpnI GATC 2 cut(s) 183, 336
DpnII GATC 2 cut(s) 181, 334
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 382
EcoRII CCWGG 2 cut(s) 198, 296
EcoT38I GRGCYC 1 cut(s) 156
FaeI CATG 4 cut(s) 107, 142, 255, 392
FaiI YATR 9 cut(s) 42, 47, 105, 140, 240, 253, 327, 390, 419
FatI CATG 4 cut(s) 103, 138, 251, 388
FauI CCCGC 1 cut(s) 56
FokI GGATG 2 cut(s) 275, 425
FriOI GRGCYC 1 cut(s) 156
FspBI CTAG 1 cut(s) 384
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 161, 361
Hin1II CATG 4 cut(s) 107, 142, 255, 392
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 3 cut(s) 203, 242, 479
HpaII CCGG 2 cut(s) 161, 361
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 2 cut(s) 181, 343
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 388, 418
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4V TGCA 2 cut(s) 11, 371
HpyF3I CTNAG 1 cut(s) 119
Hsp92II CATG 4 cut(s) 107, 142, 255, 392
Kpn2I TCCGGA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 181, 334
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 9 cut(s) 42, 174, 185, 212, 283, 310, 374, 382, 456
MaeI CTAG 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 122
MalI GATC 2 cut(s) 183, 336
MboI GATC 2 cut(s) 181, 334
MboII GAAGA 1 cut(s) 237
MflI RGATCY 2 cut(s) 181, 334
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 4 cut(s) 107, 171, 219, 488
MnlI CCTC 2 cut(s) 187, 403
MroI TCCGGA 1 cut(s) 360
MseI TTAA 1 cut(s) 449
MspI CCGG 2 cut(s) 161, 361
MspR9I CCNGG 3 cut(s) 162, 200, 298
MvaI CCWGG 2 cut(s) 200, 298
NciI CCSGG 1 cut(s) 162
NdeII GATC 2 cut(s) 181, 334
NlaIII CATG 4 cut(s) 107, 142, 255, 392
NlaIV GGNNCC 1 cut(s) 166
PfeI GAWTC 3 cut(s) 203, 242, 479
PfoI TCCNGGA 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 198, 296
PspGI CCWGG 2 cut(s) 198, 296
PspN4I GGNNCC 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 181, 334
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 449
Sau3AI GATC 2 cut(s) 181, 334
ScrFI CCNGG 3 cut(s) 162, 200, 298
SduI GDGCHC 1 cut(s) 156
SetI ASST 5 cut(s) 16, 28, 156, 401, 429
Sse9I AATT 4 cut(s) 107, 171, 219, 488
SsiI CCGC 2 cut(s) 63, 249
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 160, 198, 296
TaaI ACNGT 1 cut(s) 435
TaqI TCGA 1 cut(s) 206
TasI AATT 4 cut(s) 107, 171, 219, 488
TfiI GAWTC 3 cut(s) 203, 242, 479
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 290
TspDTI ATGAA 4 cut(s) 120, 127, 279, 405
TspRI CASTG 1 cut(s) 290
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 2 cut(s) 171, 488
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.