Rroxscaffold_4G00326760

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000466
Physical Location & Seq
Forward (+)
58970073 .. 58977803
7731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326760.1

Sequence Viewer

Length: 714 bp
ATGGCACGGAAGGATGGACGATCCAAGACTATAGAAACAAAGCTTTACCTGATATACAGCTATAGCGGTCACATTCACGCCAGGCTGCTGAGCTACATGGGCTGCGTTACATGCATTGTGGCTGCTGAACGGCACTGGATTCAAACAGTGTCATCATCATCGTCATCCTCCCATCGTCATCCATGCAAATATGAAGTATTTCTATGTTTTAGAGGGGAAGACACCGGCAACAACTTTGTGAGACATCTATATGCTGTGTTAGATCAGAGTGGAATTGTCACTTTTACAGATGACAAGAGGCTTCAGAAGGGAAAATCCATTTCTCCAGAACTTCTAAATGCTATAGAGGATTCAAAAGTTGCCCTTGTTCTTCTTTTTTCAAACCATGCTTCTTCATCATGGTCCTTGGATGAGCTTTTGACAATTATGCAATGCAAACAAGACATGGGGCGAGCGGTCCTTCCAGTCTTTTATCAGCTGCAGCCATCTTTCTGCTTGCCTCTATCTGGTCTTTGTAATTTAACCTCTCTGAACCTAAGCAACTGCAATGTTGTTGAAAGATCATTTCCCAATGACATTGGCTTCTTATTTGGTGGCATTAAATCTGAGTGGGAAATTTTTTTTATCAGTCTTCGTGCAACCATTAGATCACTAGTGAAGCTTGAAAATATTAAGTTGGAGAATTGCAGGAGACTTGAAAAGTTATCCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

237

Amino Acids

26.82

Weight (kDa)

8.71

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 63 - 164 1.8e-30 TIR domain
TIR_2 PF13676 66 - 167 1.2e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 455
AciI CCGC 2 cut(s) 66, 455
AclWI GGATC 1 cut(s) 15
AcsI RAATTY 1 cut(s) 615
AcuI CTGAAG 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 6 cut(s) 143, 354, 381, 557, 665, 698
AhlI ACTAGT 1 cut(s) 652
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 6 cut(s) 43, 60, 93, 415, 478, 661
AluI AGCT 6 cut(s) 43, 60, 93, 415, 478, 661
Alw26I GTCTC 2 cut(s) 235, 685
AlwI GGATC 1 cut(s) 15
ApeKI GCWGC 5 cut(s) 85, 102, 122, 478, 481
ApoI RAATTY 1 cut(s) 615
Asp700I GAANNNNTTC 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 402, 457
AvaII GGWCC 2 cut(s) 402, 457
BbsI GAAGAC 2 cut(s) 225, 623
BbvI GCAGC 5 cut(s) 72, 89, 109, 465, 493
BccI CCATC 3 cut(s) 8, 180, 493
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 235, 685
BcuI ACTAGT 1 cut(s) 652
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 4 cut(s) 30, 61, 342, 479
BisI GCNGC 5 cut(s) 86, 103, 123, 479, 482
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 5 cut(s) 87, 104, 124, 480, 483
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 2 cut(s) 402, 457
BmgT120I GGNCC 2 cut(s) 402, 457
BmrFI CCNGG 1 cut(s) 82
BpiI GAAGAC 2 cut(s) 225, 623
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 536
Bpu1102I GCTNAGC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 140, 464
Bse3DI GCAATG 2 cut(s) 437, 553
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 4 cut(s) 19, 164, 178, 415
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMI GCAATG 2 cut(s) 437, 553
BseMII CTCAG 2 cut(s) 80, 597
BseNI ACTGG 2 cut(s) 140, 464
BseXI GCAGC 5 cut(s) 72, 89, 109, 465, 493
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 2 cut(s) 235, 685
Bsp143I GATC 4 cut(s) 20, 262, 560, 647
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 2 cut(s) 66, 455
BspCNI CTCAG 2 cut(s) 81, 598
BspMAI CTGCAG 1 cut(s) 483
BspPI GGATC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 455
BsrDI GCAATG 2 cut(s) 437, 553
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 140, 464
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 4 cut(s) 20, 262, 560, 647
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 453, 497
BstDEI CTNAG 3 cut(s) 89, 536, 606
BstF5I GGATG 4 cut(s) 19, 164, 178, 415
BstKTI GATC 4 cut(s) 23, 265, 563, 650
BstMAI GTCTC 2 cut(s) 235, 685
BstMBI GATC 4 cut(s) 20, 262, 560, 647
BstMWI GCNNNNNNNGC 2 cut(s) 99, 111
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 4 cut(s) 30, 61, 342, 479
BstV1I GCAGC 5 cut(s) 72, 89, 109, 465, 493
BstV2I GAAGAC 2 cut(s) 225, 623
BtsCI GGATG 4 cut(s) 19, 164, 178, 415
BtsIMutI CAGTG 2 cut(s) 133, 153
Cac8I GCNNGC 2 cut(s) 453, 497
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 2 cut(s) 402, 457
CviAII CATG 6 cut(s) 97, 111, 183, 386, 399, 445
DdeI CTNAG 3 cut(s) 89, 536, 606
DpnI GATC 4 cut(s) 22, 264, 562, 649
DpnII GATC 4 cut(s) 20, 262, 560, 647
Eco130I CCWWGG 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 402, 457
Eco57I CTGAAG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 6 cut(s) 100, 114, 186, 389, 402, 448
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 6 cut(s) 96, 110, 182, 385, 398, 444
Fnu4HI GCNGC 5 cut(s) 86, 103, 123, 479, 482
FokI GGATG 4 cut(s) 26, 151, 165, 422
Fsp4HI GCNGC 5 cut(s) 86, 103, 123, 479, 482
FspBI CTAG 1 cut(s) 653
GluI GCNGC 5 cut(s) 86, 103, 123, 479, 482
GsuI CTGGAG 1 cut(s) 309
HapII CCGG 1 cut(s) 225
Hin1II CATG 6 cut(s) 100, 114, 186, 389, 402, 448
HindIII AAGCTT 2 cut(s) 41, 659
HinfI GANTC 2 cut(s) 139, 350
HpaII CCGG 1 cut(s) 225
Hpy188I TCNGA 4 cut(s) 267, 306, 531, 607
Hpy188III TCNNGA 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 4, 301, 470
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 8 cut(s) 114, 186, 430, 435, 481, 546, 638, 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 111
HpyF3I CTNAG 3 cut(s) 89, 536, 606
Hsp92II CATG 6 cut(s) 100, 114, 186, 389, 402, 448
Kzo9I GATC 4 cut(s) 20, 262, 560, 647
LpnPI CCDG 9 cut(s) 62, 67, 94, 121, 238, 339, 477, 492, 673
Lsp1109I GCAGC 5 cut(s) 72, 89, 109, 465, 493
MaeI CTAG 1 cut(s) 653
MaeIII GTNAC 3 cut(s) 68, 106, 277
MalI GATC 4 cut(s) 22, 264, 562, 649
MbiI CCGCTC 1 cut(s) 455
MboI GATC 4 cut(s) 20, 262, 560, 647
MboII GAAGA 4 cut(s) 230, 362, 384, 623
MluCI AATT 5 cut(s) 273, 423, 517, 615, 682
MmeI TCCRAC 1 cut(s) 657
MnlI CCTC 6 cut(s) 178, 206, 291, 340, 510, 535
Mph1103I ATGCAT 1 cut(s) 116
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 3 cut(s) 521, 600, 672
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 99, 111
NdeII GATC 4 cut(s) 20, 262, 560, 647
NlaIII CATG 6 cut(s) 100, 114, 186, 389, 402, 448
NmuCI GTSAC 2 cut(s) 68, 277
NsiI ATGCAT 1 cut(s) 116
NspI RCATGY 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 139, 350
PkrI GCNGC 5 cut(s) 87, 104, 124, 480, 483
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspPI GGNCC 2 cut(s) 402, 457
PstI CTGCAG 1 cut(s) 483
PvuII CAGCTG 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 3 cut(s) 521, 600, 672
SatI GCNGC 5 cut(s) 86, 103, 123, 479, 482
Sau3AI GATC 4 cut(s) 20, 262, 560, 647
Sau96I GGNCC 2 cut(s) 402, 457
ScrFI CCNGG 1 cut(s) 82
SetI ASST 9 cut(s) 45, 51, 62, 95, 417, 480, 527, 537, 663
SfcI CTRYAG 4 cut(s) 30, 61, 342, 479
SinI GGWCC 2 cut(s) 402, 457
SmiMI CAYNNNNRTG 1 cut(s) 249
SpeI ACTAGT 1 cut(s) 652
Sse9I AATT 5 cut(s) 273, 423, 517, 615, 682
SsiI CCGC 2 cut(s) 66, 455
SspI AATATT 1 cut(s) 670
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 148
TasI AATT 5 cut(s) 273, 423, 517, 615, 682
TfiI GAWTC 2 cut(s) 139, 350
Tru1I TTAA 3 cut(s) 521, 600, 672
Tru9I TTAA 3 cut(s) 521, 600, 672
TscAI CASTG 2 cut(s) 140, 153
TseFI GTSAC 2 cut(s) 68, 277
TseI GCWGC 5 cut(s) 85, 102, 122, 478, 481
Tsp45I GTSAC 2 cut(s) 68, 277
TspDTI ATGAA 2 cut(s) 207, 384
TspGWI ACGGA 2 cut(s) 22, 697
TspRI CASTG 2 cut(s) 140, 153
VpaK11BI GGWCC 2 cut(s) 402, 457
XapI RAATTY 1 cut(s) 615
XceI RCATGY 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 653
Zsp2I ATGCAT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.