RLG00000024393

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000466
Physical Location & Seq
Forward (+)
35448381 .. 35448890
510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024393

Sequence Viewer

Length: 510 bp
ATGGCCTCAAGCTCCCGACTAGCCTATGCAGCATCAATTCCTTCGTCAGCTCCTTGTTGGAAGTATGATGTGTTTTTAAGTTTTAGGGGTAAAGATACTCGCAAGGGTATTACATTCGAATTATACAATCAACTGCAAAATAAGAGTGGAATTAAAACATTCATGGATGATCAAGACCTTGAAGTAGGGGATGTTATTTCTCCTACTCTCCTTACGGCAATTGAAGAATCAAGGTTTGCAATTGTAGTTCTCTCACTAAATTATGCTTCTTCTCCATGGTGTTTGGTGGAACTTACAAAGATATGTCAATGCATGAAAGACAATAATAGAATTCTGCCACTTTTTTATCACGTCGATCCCTCTGATGTACGATATCAGAAGAGGAGTTTCGAGGAGGCTTTCACTAAGCATGAAAACTCTGGGCGACACGGAGAAGAGGTGCAGCAATGGAGAGATGCTTTGAAGAAAGTGGCCAATTTCTCTGGTTGGCATACACTGAATTACAAGTAA

Protein Analysis

170

Amino Acids

19.43

Weight (kDa)

7.67

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 167 4.8e-47 TIR domain
TIR_2 PF13676 24 - 121 5.9e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 330
AfaI GTAC 1 cut(s) 369
AgsI TTSAA 3 cut(s) 182, 224, 463
AjiI CACGTC 1 cut(s) 352
AluBI AGCT 2 cut(s) 12, 50
AluI AGCT 2 cut(s) 12, 50
AlwI GGATC 1 cut(s) 350
AoxI GGCC 2 cut(s) 3, 471
ApeKI GCWGC 2 cut(s) 29, 442
ApoI RAATTY 1 cut(s) 330
AsuII TTCGAA 1 cut(s) 117
BalI TGGCCA 1 cut(s) 473
BbvI GCAGC 2 cut(s) 41, 454
BceAI ACGGC 1 cut(s) 231
BclI TGATCA 1 cut(s) 169
BfaI CTAG 1 cut(s) 20
BisI GCNGC 2 cut(s) 30, 443
BlsI GCNGC 2 cut(s) 31, 444
BmgBI CACGTC 1 cut(s) 352
BmsI GCATC 2 cut(s) 41, 445
Bpu14I TTCGAA 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse3DI GCAATG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 2 cut(s) 172, 196
BseMI GCAATG 1 cut(s) 452
BseRI GAGGAG 2 cut(s) 397, 407
BseXI GCAGC 2 cut(s) 41, 454
BsgI GTGCAG 1 cut(s) 461
BshFI GGCC 2 cut(s) 5, 473
BsnI GGCC 2 cut(s) 5, 473
Bsp119I TTCGAA 1 cut(s) 117
Bsp143I GATC 2 cut(s) 169, 355
Bsp19I CCATGG 1 cut(s) 275
BspANI GGCC 2 cut(s) 5, 473
BspPI GGATC 1 cut(s) 350
BspT104I TTCGAA 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 2 cut(s) 169, 355
BssT1I CCWWGG 1 cut(s) 275
Bst6I CTCTTC 2 cut(s) 374, 429
BstBI TTCGAA 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 172, 196
BstKTI GATC 2 cut(s) 172, 358
BstMBI GATC 2 cut(s) 169, 355
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstV1I GCAGC 2 cut(s) 41, 454
BsuRI GGCC 2 cut(s) 5, 473
BtgI CCRYGG 1 cut(s) 275
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 2 cut(s) 172, 196
BtsIMutI CAGTG 1 cut(s) 494
Csp6I GTAC 1 cut(s) 368
CviAII CATG 4 cut(s) 163, 276, 313, 410
CviJI RGCY 6 cut(s) 5, 12, 23, 50, 398, 473
CviKI_1 RGCY 6 cut(s) 5, 12, 23, 50, 398, 473
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 1 cut(s) 405
DpnI GATC 2 cut(s) 171, 357
DpnII GATC 2 cut(s) 169, 355
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 374, 429
EarI CTCTTC 2 cut(s) 374, 429
Eco130I CCWWGG 1 cut(s) 275
Eco32I GATATC 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 330
EcoRV GATATC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 275
EcoT22I ATGCAT 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 4 cut(s) 166, 279, 316, 413
FatI CATG 4 cut(s) 162, 275, 312, 409
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 30, 443
FokI GGATG 2 cut(s) 179, 203
Fsp4HI GCNGC 2 cut(s) 30, 443
FspBI CTAG 1 cut(s) 20
GluI GCNGC 2 cut(s) 30, 443
HaeIII GGCC 2 cut(s) 5, 473
Hin1II CATG 4 cut(s) 166, 279, 316, 413
HinfI GANTC 1 cut(s) 227
Hpy188I TCNGA 2 cut(s) 364, 378
Hpy188III TCNNGA 2 cut(s) 15, 173
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 1 cut(s) 51
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 5 cut(s) 29, 136, 239, 312, 442
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 4 cut(s) 166, 279, 316, 413
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 2 cut(s) 169, 355
LmnI GCTCC 2 cut(s) 17, 55
LpnPI CCDG 2 cut(s) 405, 468
Lsp1109I GCAGC 2 cut(s) 41, 454
LweI GCATC 2 cut(s) 41, 445
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 351
MalI GATC 2 cut(s) 171, 357
MboI GATC 2 cut(s) 169, 355
MboII GAAGA 5 cut(s) 236, 261, 391, 446, 475
MfeI CAATTG 2 cut(s) 219, 240
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 9 cut(s) 36, 119, 150, 219, 240, 259, 330, 475, 499
MluNI TGGCCA 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 6 cut(s) 16, 370, 375, 385, 388, 430
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 1 cut(s) 314
MscI TGGCCA 1 cut(s) 473
MseI TTAA 2 cut(s) 77, 153
Msp20I TGGCCA 1 cut(s) 473
MunI CAATTG 2 cut(s) 219, 240
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 275
NdeII GATC 2 cut(s) 169, 355
NlaIII CATG 4 cut(s) 166, 279, 316, 413
NsiI ATGCAT 1 cut(s) 314
NspV TTCGAA 1 cut(s) 117
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 31, 444
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SaqAI TTAA 2 cut(s) 77, 153
SatI GCNGC 2 cut(s) 30, 443
Sau3AI GATC 2 cut(s) 169, 355
SetI ASST 6 cut(s) 14, 52, 180, 236, 354, 441
SfaNI GCATC 2 cut(s) 41, 445
SfuI TTCGAA 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 9 cut(s) 36, 119, 150, 219, 240, 259, 330, 475, 499
SspMI CTAG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 275
TaiI ACGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 117, 354, 390
TasI AATT 9 cut(s) 36, 119, 150, 219, 240, 259, 330, 475, 499
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 2 cut(s) 77, 153
Tru9I TTAA 2 cut(s) 77, 153
TscAI CASTG 1 cut(s) 501
TseI GCWGC 2 cut(s) 29, 442
TspDTI ATGAA 3 cut(s) 151, 329, 426
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 501
XapI RAATTY 1 cut(s) 330
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.