Rroxscaffold_3G00240330

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000466
Physical Location & Seq
Reverse (-)
32025411 .. 32026330
920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240330.1

Sequence Viewer

Length: 423 bp
ATGGCATCAAGCTCTAAAACAGCCTCTGCATCATCATCAGCTCATCAGTGGAAATATGATGTATTTTTGAGTTTCAGGGGTGCTGACACTTGCAGGAGTATCACATCCGAAATATACGACGGACTGCAGAACAAGAGAGGAATTAAAACATTCATGGATGATCAAGATCTTAGAGTAGGGGATGTTATTTCTCCCAGTCTTCTAACAGCAATTGAAGAATCAAGGTTTGCAATTGTGGTTTTCTCGCCAAACTATGCTTTTTCTACTTGGTGTTTGGAGGAACTTACAAAGATTTGTCAATGCATGGAAGACAATCGAAGCTTTCGGGATCCGACCAAAATTCCAGATTCCGGCATCCCTTCGGGCCGATCGTCGGTTTTCTGGAACGTCTTCTCCTCTAAATCACACCCATACCCACCTTGA

Protein Analysis

140

Amino Acids

15.68

Weight (kDa)

6.08

Isoelectric Point (pI)

63.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 106 4.6e-31 TIR domain
TIR_2 PF13676 21 - 103 3.9e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 323, 336
AcsI RAATTY 1 cut(s) 339
AfiI CCNNNNNNNGG 2 cut(s) 350, 373
AgsI TTSAA 1 cut(s) 215
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
AluBI AGCT 3 cut(s) 12, 41, 321
AluI AGCT 3 cut(s) 12, 41, 321
AlwI GGATC 2 cut(s) 323, 336
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 339
Asp700I GAANNNNTTC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 364
BamHI GGATCC 1 cut(s) 328
BbsI GAAGAC 3 cut(s) 191, 315, 382
BclI TGATCA 1 cut(s) 160
BfmI CTRYAG 1 cut(s) 125
BglII AGATCT 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 1 cut(s) 330
BmrI ACTGGG 1 cut(s) 189
BmsI GCATC 3 cut(s) 14, 38, 363
BmuI ACTGGG 1 cut(s) 189
BpiI GAAGAC 3 cut(s) 191, 315, 382
BsaBI GATNNNNATC 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 373
Bse1I ACTGG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 165
BseGI GGATG 4 cut(s) 104, 163, 187, 354
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 350, 373
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 371
BshFI GGCC 1 cut(s) 366
BsiEI CGRYCG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 350, 373
BsnI GGCC 1 cut(s) 366
Bsp143I GATC 4 cut(s) 160, 166, 328, 368
BspANI GGCC 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 330
BspMAI CTGCAG 1 cut(s) 129
BspPI GGATC 2 cut(s) 323, 336
BsrI ACTGG 1 cut(s) 195
BssMI GATC 4 cut(s) 160, 166, 328, 368
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 4 cut(s) 104, 163, 187, 354
BstKTI GATC 4 cut(s) 163, 169, 331, 371
BstMBI GATC 4 cut(s) 160, 166, 328, 368
BstMCI CGRYCG 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 125
BstV2I GAAGAC 3 cut(s) 191, 315, 382
BstX2I RGATCY 2 cut(s) 166, 328
BstYI RGATCY 2 cut(s) 166, 328
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 4 cut(s) 104, 163, 187, 354
BtsIMutI CAGTG 1 cut(s) 53
CaiI CAGNNNCTG 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 364
CviAII CATG 2 cut(s) 154, 304
CviJI RGCY 5 cut(s) 12, 23, 41, 321, 366
CviKI_1 RGCY 5 cut(s) 12, 23, 41, 321, 366
DdeI CTNAG 1 cut(s) 170
DpnI GATC 4 cut(s) 162, 168, 330, 370
DpnII GATC 4 cut(s) 160, 166, 328, 368
EcoT22I ATGCAT 1 cut(s) 305
FaeI CATG 2 cut(s) 157, 307
FaiI YATR 6 cut(s) 57, 115, 155, 255, 305, 412
FatI CATG 2 cut(s) 153, 303
FbaI TGATCA 1 cut(s) 160
FokI GGATG 4 cut(s) 91, 170, 194, 341
HaeIII GGCC 1 cut(s) 366
HapII CCGG 1 cut(s) 351
Hin1II CATG 2 cut(s) 157, 307
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 2 cut(s) 218, 347
HpaII CCGG 1 cut(s) 351
Hpy188I TCNGA 2 cut(s) 109, 333
Hpy188III TCNNGA 4 cut(s) 164, 326, 344, 382
Hpy99I CGWCG 2 cut(s) 122, 376
HpyAV CCTTC 1 cut(s) 369
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 5 cut(s) 29, 93, 127, 230, 303
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 2 cut(s) 157, 307
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 4 cut(s) 160, 166, 328, 368
LpnPI CCDG 6 cut(s) 61, 79, 208, 357, 364, 367
LweI GCATC 3 cut(s) 14, 38, 363
MaeII ACGT 1 cut(s) 387
MalI GATC 4 cut(s) 162, 168, 330, 370
MboI GATC 4 cut(s) 160, 166, 328, 368
MboII GAAGA 4 cut(s) 191, 227, 320, 382
MfeI CAATTG 2 cut(s) 210, 231
MflI RGATCY 2 cut(s) 166, 328
MluCI AATT 4 cut(s) 141, 210, 231, 339
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 4 cut(s) 34, 131, 271, 406
Mph1103I ATGCAT 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 1 cut(s) 144
MspI CCGG 1 cut(s) 351
MunI CAATTG 2 cut(s) 210, 231
NdeII GATC 4 cut(s) 160, 166, 328, 368
NlaIII CATG 2 cut(s) 157, 307
NlaIV GGNNCC 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 2 cut(s) 218, 347
Ple19I CGATCG 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 2 cut(s) 166, 328
PvuI CGATCG 1 cut(s) 371
SaqAI TTAA 1 cut(s) 144
Sau3AI GATC 4 cut(s) 160, 166, 328, 368
Sau96I GGNCC 1 cut(s) 364
SetI ASST 6 cut(s) 14, 43, 227, 323, 390, 421
SfaNI GCATC 3 cut(s) 14, 38, 363
SfcI CTRYAG 1 cut(s) 125
Sse9I AATT 4 cut(s) 141, 210, 231, 339
TaiI ACGT 1 cut(s) 390
TaqI TCGA 1 cut(s) 316
TasI AATT 4 cut(s) 141, 210, 231, 339
TfiI GAWTC 2 cut(s) 218, 347
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 142
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 53
XapI RAATTY 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.