Rroxscaffold_6G00408110

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000466
Physical Location & Seq
Reverse (-)
30608105 .. 30608926
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408110.1

Sequence Viewer

Length: 822 bp
ATGAGGATGATTATTGTGATTAGTGTGGTAATGGTGCAAAGGTTCGGTGTCATAGCCAGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTAATTGTGACTTGGAACGTAATAGCCAGTGTTGGGTGGATATGGAACAGTAGGTTCAGTTACATGGTTGAAACCCCAGAGCCAACATCAGAGGATAATCAAGAACTGGTTGACGTCGTTGAAATCCCAGAGCCAATATCAGAGGATAATCAAGAACTGGTCGACGTCGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACCGGTTGACGTCGATGTCTCTGCATCTCTTCCTTCATCATTAGCTGAATCATCAGCTCCTAGGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGCAGACACTCGTAAGGGTATTACCTCCTTTTTATACGATCGACTGCAAAACAGAGGAATCAAAACATTCATGGACGACCAGGACCTTCAAGTAGGGAATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAGTTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGGAAGAAGACAAAAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGACATCAGAAGAGGAGTTTCGGAGATGCTTTCTCTAAGCATGAAAAATTTGCGCGACACAGATCAAAGAAGCTGCAGCAGTGGAGAGATGCTTTAACAAAAGTGGCAAATTTCTCCGGGTGGCATACACTGAATTATAAGTAA

Protein Analysis

273

Amino Acids

31.62

Weight (kDa)

5.25

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 124 - 271 3.5e-49 TIR domain
TIR_2 PF13676 127 - 237 5.8e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 3 cut(s) 213, 264, 315
AccI GTMKAC 1 cut(s) 258
AccII CGCG 1 cut(s) 733
AclWI GGATC 2 cut(s) 659, 672
AcsI RAATTY 3 cut(s) 639, 725, 787
AcuI CTGAAG 1 cut(s) 376
AcyI GRCGYC 3 cut(s) 210, 261, 312
AdeI CACNNNGTG 1 cut(s) 585
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 1 cut(s) 129
AflII CTTAAG 1 cut(s) 62
AgeI ACCGGT 1 cut(s) 304
AgsI TTSAA 4 cut(s) 167, 218, 269, 488
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 4 cut(s) 347, 359, 390, 751
AluI AGCT 4 cut(s) 347, 359, 390, 751
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 2 cut(s) 659, 672
ApeKI GCWGC 2 cut(s) 751, 754
ApoI RAATTY 3 cut(s) 639, 725, 787
AsiGI ACCGGT 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 83
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 1 cut(s) 733
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 1 cut(s) 796
AvaII GGWCC 1 cut(s) 481
AvrII CCTAGG 1 cut(s) 362
BamHI GGATCC 1 cut(s) 664
BbsI GAAGAC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 738, 766
BceAI ACGGC 1 cut(s) 537
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 479
BcnI CCSGG 1 cut(s) 796
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 752
BfrI CTTAAG 1 cut(s) 62
BisI GCNGC 2 cut(s) 752, 755
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 2 cut(s) 753, 756
Bme1390I CCNGG 2 cut(s) 479, 796
Bme18I GGWCC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 1 cut(s) 666
BmrFI CCNGG 2 cut(s) 479, 796
BmsI GCATC 3 cut(s) 335, 694, 757
BpiI GAAGAC 1 cut(s) 633
BpuMI CCSGG 1 cut(s) 796
BsaHI GRCGYC 3 cut(s) 210, 261, 312
BsaJI CCNNGG 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 304
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 4 cut(s) 57, 123, 207, 258
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 129
BseNI ACTGG 4 cut(s) 57, 123, 207, 258
BseRI GAGGAG 1 cut(s) 706
BseXI GCAGC 2 cut(s) 738, 766
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 1 cut(s) 733
Bsh1285I CGRYCG 1 cut(s) 439
BshTI ACCGGT 1 cut(s) 304
BsiEI CGRYCG 1 cut(s) 439
BsiSI CCGG 2 cut(s) 305, 795
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 620
Bsp143I GATC 3 cut(s) 436, 664, 740
BspFNI CGCG 1 cut(s) 733
BspLI GGNNCC 1 cut(s) 666
BspMAI CTGCAG 1 cut(s) 756
BspPI GGATC 2 cut(s) 659, 672
BspTI CTTAAG 1 cut(s) 62
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 4 cut(s) 57, 123, 207, 258
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 3 cut(s) 436, 664, 740
BssNI GRCGYC 3 cut(s) 210, 261, 312
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 3 cut(s) 336, 580, 683
BstACI GRCGYC 3 cut(s) 210, 261, 312
BstAFI CTTAAG 1 cut(s) 62
BstDEI CTNAG 2 cut(s) 599, 714
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 733
BstHHI GCGC 1 cut(s) 733
BstKTI GATC 3 cut(s) 439, 667, 743
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 3 cut(s) 436, 664, 740
BstMCI CGRYCG 1 cut(s) 439
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 477, 794
BstSFI CTRYAG 1 cut(s) 752
BstUI CGCG 1 cut(s) 733
BstV1I GCAGC 2 cut(s) 738, 766
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BtsCI GGATG 1 cut(s) 12
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 3 cut(s) 130, 764, 806
CfoI GCGC 1 cut(s) 733
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 481
Csp6I GTAC 1 cut(s) 677
CspAI ACCGGT 1 cut(s) 304
CviAII CATG 5 cut(s) 160, 374, 469, 619, 719
CviJI RGCY 9 cut(s) 56, 122, 178, 229, 280, 347, 359, 390, 751
CviKI_1 RGCY 9 cut(s) 56, 122, 178, 229, 280, 347, 359, 390, 751
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 2 cut(s) 599, 714
DpnI GATC 3 cut(s) 438, 666, 742
DpnII GATC 3 cut(s) 436, 664, 740
DraIII CACNNNGTG 1 cut(s) 585
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
Eam1104I CTCTTC 3 cut(s) 336, 580, 683
EarI CTCTTC 3 cut(s) 336, 580, 683
Eco130I CCWWGG 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 481
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 5 cut(s) 163, 377, 472, 622, 722
FatI CATG 5 cut(s) 159, 373, 468, 618, 718
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 752, 755
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 752, 755
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 732
GluI GCNGC 2 cut(s) 752, 755
HapII CCGG 2 cut(s) 305, 795
HhaI GCGC 1 cut(s) 733
Hin1I GRCGYC 3 cut(s) 210, 261, 312
Hin1II CATG 5 cut(s) 163, 377, 472, 622, 722
Hin6I GCGC 1 cut(s) 731
HinP1I GCGC 1 cut(s) 731
HincII GTYRAC 3 cut(s) 208, 259, 310
HindII GTYRAC 3 cut(s) 208, 259, 310
HinfI GANTC 3 cut(s) 350, 456, 533
HpaII CCGG 2 cut(s) 305, 795
Hpy166II GTNNAC 3 cut(s) 208, 259, 310
Hpy188I TCNGA 5 cut(s) 187, 238, 289, 687, 701
Hpy188III TCNNGA 3 cut(s) 197, 248, 299
Hpy8I GTNNAC 3 cut(s) 208, 259, 310
Hpy99I CGWCG 4 cut(s) 215, 263, 266, 317
HpyAV CCTTC 2 cut(s) 345, 494
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 4 cut(s) 114, 210, 261, 312
HpyCH4V TGCA 7 cut(s) 37, 326, 401, 445, 545, 618, 754
HpyF3I CTNAG 2 cut(s) 599, 714
HpySE526I ACGT 4 cut(s) 114, 210, 261, 312
Hsp92I GRCGYC 3 cut(s) 210, 261, 312
Hsp92II CATG 5 cut(s) 163, 377, 472, 622, 722
HspAI GCGC 1 cut(s) 731
Kzo9I GATC 3 cut(s) 436, 664, 740
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 2 cut(s) 738, 766
LweI GCATC 3 cut(s) 335, 694, 757
MaeI CTAG 1 cut(s) 363
MaeII ACGT 4 cut(s) 114, 210, 261, 312
MaeIII GTNAC 2 cut(s) 103, 155
MalI GATC 3 cut(s) 438, 666, 742
MboI GATC 3 cut(s) 436, 664, 740
MboII GAAGA 6 cut(s) 88, 323, 567, 635, 638, 700
MflI RGATCY 1 cut(s) 664
MluCI AATT 7 cut(s) 79, 99, 525, 639, 725, 787, 811
MnlI CCTC 8 cut(s) 181, 232, 283, 433, 446, 583, 586, 684
Mph1103I ATGCAT 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 3 cut(s) 63, 528, 773
MslI CAYNNNNRTG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 62
MspI CCGG 2 cut(s) 305, 795
MspR9I CCNGG 2 cut(s) 479, 796
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 733
NciI CCSGG 1 cut(s) 796
NdeII GATC 3 cut(s) 436, 664, 740
NlaIII CATG 5 cut(s) 163, 377, 472, 622, 722
NlaIV GGNNCC 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 620
PctI GAATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 3 cut(s) 350, 456, 533
PinAI ACCGGT 1 cut(s) 304
PkrI GCNGC 2 cut(s) 753, 756
Ple19I CGATCG 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 481
PsiI TTATAA 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 1 cut(s) 666
PspPI GGNCC 1 cut(s) 481
PspPPI RGGWCCY 1 cut(s) 481
PstI CTGCAG 1 cut(s) 756
PsuI RGATCY 1 cut(s) 664
PvuI CGATCG 1 cut(s) 439
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
RseI CAYNNNNRTG 1 cut(s) 92
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 3 cut(s) 63, 528, 773
SatI GCNGC 2 cut(s) 752, 755
Sau3AI GATC 3 cut(s) 436, 664, 740
Sau96I GGNCC 1 cut(s) 481
ScrFI CCNGG 2 cut(s) 479, 796
SfaNI GCATC 3 cut(s) 335, 694, 757
SfcI CTRYAG 1 cut(s) 752
SinI GGWCC 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 7 cut(s) 79, 99, 525, 639, 725, 787, 811
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 477, 794
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 4 cut(s) 117, 213, 264, 315
TaqI TCGA 3 cut(s) 258, 315, 439
TasI AATT 7 cut(s) 79, 99, 525, 639, 725, 787, 811
TfiI GAWTC 3 cut(s) 350, 456, 533
Tru1I TTAA 3 cut(s) 63, 528, 773
Tru9I TTAA 3 cut(s) 63, 528, 773
TscAI CASTG 3 cut(s) 130, 764, 813
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 2 cut(s) 751, 754
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 4 cut(s) 76, 327, 457, 735
TspRI CASTG 3 cut(s) 130, 764, 813
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 3 cut(s) 639, 725, 787
XcmI CCANNNNNNNNNTGG 1 cut(s) 586
XmaJI CCTAGG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 1 cut(s) 363
ZraI GACGTC 3 cut(s) 211, 262, 313
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.