Rroxscaffold_1G00003240

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000466
Physical Location & Seq
Forward (+)
3797005 .. 3802374
5370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003240.1

Sequence Viewer

Length: 1254 bp
ATGGAATTCTATTACTTCCAGATGGCCATGGCGATTATTGTGGTAATTGTGACATGGATCGGTGTCATAGCTACTATTAAGTGGATATGGACTCTAGTGAAGCATCGGTTGGGTTACATGGTTGAAAGCATGAGCCAACATCGCAGGGTTTTCGTCAACATCGATGCCTCCAGACCTCTTCCTTCACCATCAGCTGAATCATCTGCAGCTCGTCGATGGAATTATGATGTGTTCTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATTACATTTGAATTATATGATCGACTGCACAGGAGGGGAATTAAAATATTCATGGATGACCAAGACCTTCAAGTAGGGGATACTATTTCTCCTACTCTACTAAAAGCAATTGAAGAATCAAGGTTGGCAATTGTTGTTCTGTCGGAAAAGTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTACAAAGATCTGTGAGTGCATGAAAGACGACAACAGAATTCTTCCACTTTTTTATAATGTCGAGCCTACTGATGTAAGATATCAGAAGAGAAGTTTCGAAGAAGCTTTCACCAAATATGAAAATTCTGGGCGACATAGATCGGAGAAGGTCCAACAATGGAGAGATGCTTTAAACAAAGTGGCTCATTTCTCTGGTTGGGATTCAAAGAAATACAAGACTGAAAGAGAACTCATCGAAGACATTGTGGAATATGTCTGTAGTAAAGTACAACCTATTGCAATCAACATGGGAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGAATATGGTTATGAAAGGGCTAGAAGACGACGAGGTCACTGCCATTGGAGTCTACGGAATGGGAGGTGTCGGCAAGACAACCATGGTAAAACATATCAGTGATCAAGCTCGAAAAAATGGAATCTTTCGTCATGTAATTATGGCTACTCTATCACAAAGCCCTGACTTGAGGAAAATTCAAGGCCAATTGGCAGATCTGTTGGGCTTCGAATTCATGGAGGAGACTGAAATTGGAAGAGCAGCTAGATTGAGTAAGGAGATAATGAGAAGAAATAAGATCCTCATAATCCTTGATGACATTTGGGAGAGGATGGATATATCAAGAATAGGAATACCTAGCTACGAGGAACTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACAAGGATATGGAATGTCTGTCATGTCATGAGATGCCAAGAAAAGATCACCCCTCAATATCCTATCAAAAGAGGATTCTTTGGACATTGTTTGTGA

Protein Analysis

417

Amino Acids

48.56

Weight (kDa)

8.29

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 74 - 244 2.2e-49 TIR domain
TIR_2 PF13676 77 - 172 1.9e-16 TIR domain
NB-ARC PF00931 257 - 401 1.8e-23 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 1 cut(s) 809
AccI GTMKAC 1 cut(s) 828
AclWI GGATC 2 cut(s) 65, 1048
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 6 cut(s) 5, 486, 571, 752, 951, 986
AfaI GTAC 1 cut(s) 717
AgsI TTSAA 9 cut(s) 125, 278, 338, 380, 654, 749, 758, 956, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 7 cut(s) 71, 194, 209, 554, 884, 1019, 1116
AluI AGCT 7 cut(s) 71, 194, 209, 554, 884, 1019, 1116
Alw26I GTCTC 1 cut(s) 992
AlwI GGATC 2 cut(s) 65, 1048
AoxI GGCC 2 cut(s) 24, 958
ApeKI GCWGC 2 cut(s) 206, 1016
ApoI RAATTY 6 cut(s) 5, 486, 571, 752, 951, 986
AspS9I GGNCC 2 cut(s) 248, 598
AsuHPI GGTGA 4 cut(s) 177, 550, 1147, 1198
AsuII TTCGAA 2 cut(s) 546, 984
AvaII GGWCC 2 cut(s) 248, 598
BalI TGGCCA 1 cut(s) 26
BbsI GAAGAC 2 cut(s) 693, 807
BbvI GCAGC 2 cut(s) 218, 1028
BccI CCATC 4 cut(s) 16, 196, 210, 1081
BcgI CGANNNNNNTGC 4 cut(s) 133, 167, 797, 831
BciVI GTATCC 1 cut(s) 340
BclI TGATCA 1 cut(s) 877
BcoDI GTCTC 1 cut(s) 992
BfaI CTAG 4 cut(s) 95, 797, 1020, 1113
BfmI CTRYAG 2 cut(s) 204, 706
BfuI GTATCC 1 cut(s) 340
BglII AGATCT 2 cut(s) 456, 970
BisI GCNGC 2 cut(s) 207, 1017
BlsI GCNGC 2 cut(s) 208, 1018
Bme18I GGWCC 2 cut(s) 248, 598
BmgT120I GGNCC 2 cut(s) 248, 598
BmiI GGNNCC 1 cut(s) 249
BmsI GCATC 4 cut(s) 112, 154, 604, 1181
BpiI GAAGAC 2 cut(s) 693, 807
BpmI CTGGAG 1 cut(s) 154
Bpu14I TTCGAA 2 cut(s) 546, 984
BpuEI CTTGAG 2 cut(s) 256, 964
Bsa29I ATCGAT 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 27, 858
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 27, 858
BseGI GGATG 2 cut(s) 328, 1092
BseRI GAGGAG 1 cut(s) 1010
BseXI GCAGC 2 cut(s) 218, 1028
BsgI GTGCAG 1 cut(s) 278
BshFI GGCC 2 cut(s) 26, 960
BshVI ATCGAT 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 992
BsnI GGCC 2 cut(s) 26, 960
Bsp119I TTCGAA 2 cut(s) 546, 984
Bsp143I GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
Bsp19I CCATGG 2 cut(s) 27, 858
BspANI GGCC 2 cut(s) 26, 960
BspDI ATCGAT 1 cut(s) 162
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 2 cut(s) 65, 1048
BspQI GCTCTTC 1 cut(s) 1006
BspT104I TTCGAA 2 cut(s) 546, 984
BssECI CCNNGG 2 cut(s) 27, 858
BssMI GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
BssT1I CCWWGG 2 cut(s) 27, 858
Bst6I CTCTTC 3 cut(s) 183, 530, 1006
BstBI TTCGAA 2 cut(s) 546, 984
BstDSI CCRYGG 2 cut(s) 27, 858
BstF5I GGATG 2 cut(s) 328, 1092
BstKTI GATC 8 cut(s) 60, 289, 459, 590, 880, 973, 1056, 1206
BstMAI GTCTC 1 cut(s) 992
BstMBI GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstSFI CTRYAG 2 cut(s) 204, 706
BstV1I GCAGC 2 cut(s) 218, 1028
BstV2I GAAGAC 2 cut(s) 693, 807
BstX2I RGATCY 3 cut(s) 456, 970, 1053
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 3 cut(s) 456, 970, 1053
Bsu15I ATCGAT 1 cut(s) 162
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 26, 960
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 2 cut(s) 27, 858
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 2 cut(s) 328, 1092
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 2 cut(s) 813, 880
CciI TCATGA 1 cut(s) 1185
Cfr13I GGNCC 2 cut(s) 248, 598
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 716
CviQI GTAC 1 cut(s) 716
DpnI GATC 8 cut(s) 59, 288, 458, 589, 879, 972, 1055, 1205
DpnII GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
DraI TTTAAA 1 cut(s) 621
DrdI GACNNNNNNGTC 1 cut(s) 809
DseDI GACNNNNNNGTC 1 cut(s) 809
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 3 cut(s) 183, 530, 1006
EarI CTCTTC 3 cut(s) 183, 530, 1006
Eco130I CCWWGG 2 cut(s) 27, 858
Eco32I GATATC 1 cut(s) 530
Eco47I GGWCC 2 cut(s) 248, 598
EcoO109I RGGNCCY 1 cut(s) 248
EcoRI GAATTC 3 cut(s) 5, 486, 986
EcoRV GATATC 1 cut(s) 530
EcoT14I CCWWGG 2 cut(s) 27, 858
ErhI CCWWGG 2 cut(s) 27, 858
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 2 cut(s) 207, 1017
FokI GGATG 2 cut(s) 335, 1099
Fsp4HI GCNGC 2 cut(s) 207, 1017
FspBI CTAG 4 cut(s) 95, 797, 1020, 1113
GluI GCNGC 2 cut(s) 207, 1017
GsuI CTGGAG 1 cut(s) 154
HaeIII GGCC 2 cut(s) 26, 960
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 7 cut(s) 91, 197, 383, 650, 825, 897, 1233
HphI GGTGA 4 cut(s) 177, 550, 1147, 1198
Hpy166II GTNNAC 2 cut(s) 157, 829
Hpy188I TCNGA 3 cut(s) 412, 534, 592
Hpy188III TCNNGA 6 cut(s) 19, 171, 235, 251, 1098, 1186
Hpy8I GTNNAC 2 cut(s) 157, 829
Hpy99I CGWCG 2 cut(s) 216, 809
HpyAV CCTTC 3 cut(s) 192, 344, 589
HpyCH4V TGCA 5 cut(s) 206, 295, 468, 728, 1137
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
LguI GCTCTTC 1 cut(s) 1006
LpnPI CCDG 9 cut(s) 32, 130, 184, 229, 264, 283, 561, 627, 951
Lsp1109I GCAGC 2 cut(s) 218, 1028
LweI GCATC 4 cut(s) 112, 154, 604, 1181
MaeI CTAG 4 cut(s) 95, 797, 1020, 1113
MaeIII GTNAC 3 cut(s) 49, 113, 811
MalI GATC 8 cut(s) 59, 288, 458, 589, 879, 972, 1055, 1205
MboI GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
MfeI CAATTG 3 cut(s) 375, 396, 962
MflI RGATCY 3 cut(s) 456, 970, 1053
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 2 cut(s) 85, 834
MmeI TCCRAC 2 cut(s) 390, 625
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
MseI TTAA 3 cut(s) 78, 309, 620
MslI CAYNNNNRTG 2 cut(s) 779, 873
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 194
MunI CAATTG 3 cut(s) 375, 396, 962
MwoI GCNNNNNNNGC 1 cut(s) 141
NcoI CCATGG 2 cut(s) 27, 858
NdeII GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 2 cut(s) 49, 811
NspV TTCGAA 2 cut(s) 546, 984
PagI TCATGA 1 cut(s) 1185
PciSI GCTCTTC 1 cut(s) 1006
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 5 cut(s) 197, 383, 650, 897, 1233
PflFI GACNNNGTC 1 cut(s) 809
PkrI GCNGC 2 cut(s) 208, 1018
PleI GAGTC 2 cut(s) 85, 833
PpsI GAGTC 2 cut(s) 85, 833
PpuMI RGGWCCY 1 cut(s) 248
PsiI TTATAA 1 cut(s) 504
Psp5II RGGWCCY 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 248, 598
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 3 cut(s) 456, 970, 1053
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 1 cut(s) 717
RsaNI GTAC 1 cut(s) 716
RseI CAYNNNNRTG 2 cut(s) 779, 873
SapI GCTCTTC 1 cut(s) 1006
SaqAI TTAA 3 cut(s) 78, 309, 620
SatI GCNGC 2 cut(s) 207, 1017
Sau3AI GATC 8 cut(s) 57, 286, 456, 587, 877, 970, 1053, 1203
Sau96I GGNCC 2 cut(s) 248, 598
SchI GAGTC 2 cut(s) 85, 834
SfaNI GCATC 4 cut(s) 112, 154, 604, 1181
SfcI CTRYAG 2 cut(s) 204, 706
SfuI TTCGAA 2 cut(s) 546, 984
SinI GGWCC 2 cut(s) 248, 598
SmiMI CAYNNNNRTG 2 cut(s) 779, 873
SmlI CTYRAG 2 cut(s) 235, 943
SmoI CTYRAG 2 cut(s) 235, 943
SspI AATATT 1 cut(s) 315
SspMI CTAG 4 cut(s) 95, 797, 1020, 1113
StyI CCWWGG 2 cut(s) 27, 858
TaqI TCGA 8 cut(s) 162, 214, 289, 510, 546, 684, 886, 984
TatI WGTACW 1 cut(s) 715
TfiI GAWTC 5 cut(s) 197, 383, 650, 897, 1233
Tru1I TTAA 3 cut(s) 78, 309, 620
Tru9I TTAA 3 cut(s) 78, 309, 620
TscAI CASTG 2 cut(s) 820, 880
TseFI GTSAC 2 cut(s) 49, 811
TseI GCWGC 2 cut(s) 206, 1016
Tsp45I GTSAC 2 cut(s) 49, 811
TspDTI ATGAA 6 cut(s) 307, 485, 582, 791, 803, 979
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 2 cut(s) 820, 880
Tth111I GACNNNGTC 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 248, 598
XapI RAATTY 6 cut(s) 5, 486, 571, 752, 951, 986
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 4 cut(s) 95, 797, 1020, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.