AT1G80070

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000798
Physical Location & Seq
Forward (+)
30117847 .. 30127811
9965 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80070.1

Sequence Viewer

Length: 7080 bp
ATGTGGAACAACAACGATGGTATGCCCTTAGCTCCTCCTGGTACTGGCGGGTCCATGATGCCGCCACCTCCCGCTGCTCATCCTTCTTACACGGCGCTTCCGCCGCCTTCTAATCCGACTCCTCCGGTCGAGCCTACTCCTGAGGAAGCGGAGGCTAAGCTTGAGGAGAAGGCCCGAAAATGGATGCAGCTAAATTCCAAACGGTACGGCGACAAGAGGAAGTTTGGATTCGTTGAAACTCAGAAGGAGGACATGCCACCTGAGCACGTCAGGAAGATCATCAGAGATCATGGTGATATGTCATCCAAGAAATTCCGTCACGACAAACGTGTATACCTTGGAGCCCTTAAATTCGTCCCTCACGCAGTTTTCAAGCTCTTGGAGAACATGCCTATGCCCTGGGAGCAGGTCCGAGATGTAAAAGTTTTGTACCACATCACCGGAGCTATTACATTTGTGAACGAGATACCATGGGTTGTGGAACCTATTTACATGGCTCAGTGGGGTACTATGTGGATTATGATGCGAAGGGAGAAGAGGGATCGTCGTCATTTCAAGAGAATGCGGTTCCCACCTTTTGATGATGAGGAGCCTCCACTAGACTATGCAGACAACTTGCTGGACGTGGATCCTCTGGAGCCAATTCAATTGGAGCTAGATGAGGAAGAAGATTCTGCTGTCCACACTTGGTTTTACGACCACAAGCCACTCGTTAAAACAAAACTGATTAATGGTCCCAGTTACCGGAGGTGGAACCTCTCACTTCCAATCATGGCAACCCTTCACAGGCTTGCAGGCCAGTTGCTGTCTGATTTGATTGATCGCAACTACTTCTACTTATTTGACATGCCATCTTTCTTCACAGCAAAAGCTCTGAACATGTGCATACCTGGTGGACCCAAGTTTGAACCTTTGTACCGTGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATCAACAAGCTCATTATTCGTTCCCCTCTGCGTACTGAGTACAGAATAGCTTTTCCCCATCTCTACAATAATCGGCCCAGGAAAGTAAAACTCTGTGTGTATCACAGTCCTATGATCATGTATATAAAGACCGAGGATCCCGATCTTCCTGCATTTTATTATGATCCGTTGATTCACCCAATAAGCAACACCAACAAGGAAAAACGTGAAAGGAAGGTTTATGATGATGAGGATGACTTCGCCTTACCAGAAGGTGTGGAACCTTTGCTAAGAGATACCCAACTCTACACTGACACTACAGCTGCTGGCATATCATTGTTGTTTGCCCCACGACCTTTCAATATGAGGTCTGGGAGGACGCGCCGCGCAGAAGATATTCCCTTGGTGTCAGAGTGGTTCAAAGAGCACTGTCCTCCAGCCTATCCGGTTAAAGTTCGGGTTAGTTACCAAAAGCTATTGAAGTGCTATGTGCTGAATGAGTTGCACCATAGACCGCCTAAAGCTCAGAAGAAGAAGCACTTGTTTCGATCTCTTGCAGCTACAAAGTTTTTCCAAAGTACCGAACTGGATTGGGTTGAAGTGGGTCTGCAAGTCTGTCGGCAGGGATATAATATGCTGAATCTGCTGATTCATAGGAAGAATTTAAACTACCTGCATCTTGACTACAACTTCAACCTCAAGCCTGTGAAAACTTTGACCACCAAAGAGCGAAAGAAGTCACGTTTTGGGAACGCTTTCCATCTGTGCCGTGAGATCCTCCGCCTGACAAAACTTGTAGTTGATGCAAATGTCCAATTCCGACTTGGTAATGTCGATGCCTTCCAATTGGCTGATGGTCTGCAGTATATTTTCTCTCATGTTGGCCAGTTGACGGGTATGTATCGTTATAAATACAGACTGATGAGGCAGATTCGGATGTGCAAGGATTTAAAGCACTTGATATATTACCGTTTCAATACTGGTCCGGTGGGTAAGGGTCCAGGGTGTGGATTTTGGGCTCCAATGTGGAGGGTATGGTTGTTTTTCCTTCGGGGTATAGTTCCTCTTCTTGAAAGATGGTTAGGGAATCTTCTTGCTCGTCAATTTGAGGGGCGTCATTCAAAAGGAGTGGCCAAAACAGTCACAAAGCAGAGAGTTGAAAGCCATTTTGATCTGGAGCTTCGGGCTGCTGTCATGCATGACGTCCTTGATGCTATGCCAGAGGGTATCAAGCAAAATAAAGCGCGGACTATATTGCAGCACCTTAGTGAGGCATGGCGGTGCTGGAAAGCTAATATCCCATGGAAGGTCCCTGGGTTACCTGTGCCGATTGAAAATATGATCCTGCGTTATGTCAAGTCTAAGGCTGATTGGTGGACGAATGTTGCTCACTACAATCGTGAGCGTATCCGAAGAGGGGCTACTGTTGATAAGACAGTTTGTAGAAAGAATCTCGGAAGGTTGACTCGTCTTTGGCTAAAGGCAGAACAGGAACGACAGCACAATTACCTGAAAGATGGTCCGTATGTCACTCCAGAAGAAGCACTTGCAATTTATACAACTACTGTTCATTGGCTGGAATCAAGGAAGTTCTCACCAATTCCATTCCCTCCATTGTCATACAAACATGACACAAAGTTGCTCATCCTTGCCCTTGAGAGACTGAAGGAATCATATAGTGTAGCTGTGAGGTTGAACCAGCAGCAGCGAGAAGAGCTTGGTTTGATTGAACAAGCTTATGACAATCCTCATGAAGCTCTATCACGAATAAAACGTCATCTTCTCACCCAACGAGGCTTTAAAGAGGTTGGCATAGAATTTATGGATCTTTATAGCTACTTGATTCCAGTTTATGAAATAGAGCCTCTAGAGAAAATTACCGATGCATATCTTGATCAATACTTGTGGTACGAAGGTGACAAGCGTCACTTGTTTCCAAACTGGATTAAGCCAGCAGACTCAGAGCCGCCCCCACTTCTGGTTTATAAATGGTGTCAAGGTATCAACAATTTGCAAGGCATATGGGACACAGGTGATGGGCAGTGTGTGGTGATGCTCCAAACTAAGTTCGAAAAGTTCTTTGAGAAAATCGATTTGACAATGTTGAACAGGCTTCTTCGTTTGGTTCTTGACCACAATATTGCAGACTATGTGTCCGCCAAGAATAATGTGGTCCTGTCTTACAAGGATATGAGTCATACTAACTCGTATGGTCTCATTAGGGGACTTCAGTTTGCATCGTTTGTTGTTCAGTTTTATGGACTATTGCTTGATCTTTTGCTTCTTGGTTTGACTCGAGCGAGTGAAATTGCTGGGCCACCTCAGATGCCTAATGAGTTTATGACGTTTTGGGATACAAAAGTGGAGACACGCCATCCCATAAGACTCTATTCTCGGTATATAGACAAAGTTCATATAATGTTTAAATTTACCCATGAAGAAGCTCGGGATCTTATTCAGCGATATCTTACGGAGCATCCCGATCCCAACAATGAAAACATGGTGGGATACAACAATAAGAAGTGCTGGCCAAGAGATGCAAGGATGAGACTGATGAAACATGATGTCAATCTTGGTAGAAGTGTCTTCTGGGACATGAAGAACCGTCTTCCTCGGAGCATCACTACTTTGGAGTGGGAGAATGGCTTTGTCTCTGTCTATAGCAAGGATAATCCTAACCTGCTCTTTAGCATGTGTGGATTTGAAGTCCGGATACTCCCTAAAATCAGGATGACCCAAGAAGCTTTCAGCAACACAAAAGATGGTGTCTGGAATCTGCAAAATGAACAGACGAAGGAGAGGACTGCAGTAGCTTTTCTGCGGGTTGACGATGAACACATGAAGGTATTTGAGAATCGTGTGAGGCAGATCCTCATGTCATCAGGATCAACCACCTTTACCAAGATCGTCAACAAATGGAATACAGCTCTAATTGGTCTCATGACTTACTTCCGGGAAGCCACTGTACACACGCAAGAGCTCTTGGATCTACTGGTCAAGTGTGAGAATAAAATCCAAACCAGGATTAAGATTGGGCTGAACTCAAAGATGCCTAGTCGATTTCCTCCTGTTATCTTCTACACTCCAAAGGAAATTGGTGGGCTGGGAATGTTGTCAATGGGACATATTTTGATCCCACAAAGTGACCTTCGGTACAGCAAGCAAACAGATGTTGGTGTAACCCACTTTAGGAGTGGAATGAGCCACGAGGAAGATCAGCTTATACCAAATCTTTACCGTTACATACAGCCGTGGGAAAGTGAATTTATTGATTCACAGCGGGTGTGGGCAGAATATGCTTTGAAGAGGCAGGAAGCCCAAGCCCAAAATAGGCGTCTTACACTGGAAGATCTGGAAGATTCTTGGGATAGGGGAATTCCTCGTATAAATACACTGTTTCAGAAGGATAGGCACACTTTGGCATATGATAAAGGTTGGAGGGTTCGGACCGACTTCAAGCAGTACCAAGTCCTTAAACAAAATCCATTCTGGTGGACACACCAGAGACATGATGGGAAGTTGTGGAACTTGAACAATTACCGCACTGATGTCATCCAAGCTCTTGGAGGTGTTGAGGGTATTCTTGAGCACACATTATTCAAGGGGACATATTTCCCGACATGGGAGGGACTTTTCTGGGAGAAGGCGTCTGGTTTTGAGGAGTCGATGAAATATAAGAAGTTGACTAATGCTCAGAGGTCTGGTCTGAACCAGATTCCAAATAGAAGATTCACGCTTTGGTGGTCGCCAACAATTAATCGAGCGAATGTCTATGTGGGTTTCCAAGTGCAGTTGGATCTGACAGGAATATTTATGCATGGAAAGATTCCTACACTTAAGATATCTTTGATTCAGATTTTCCGTGCCCATTTGTGGCAGAAGATCCATGAGAGTGTTGTTATGGATCTCTGCCAAGTATTGGACCAAGAGTTGGACGCTTTGGAGATAGAAACTGTACAGAAGGAGACAATTCATCCAAGGAAGAGTTATAAAATGAACAGCTCTTGTGCTGATGTTCTTCTATTTGCTGCTCATAAATGGCCAATGTCTAAGCCTAGCCTGGTTGCAGAGTCGAAGGATATGTTCGACCAGAAAGCGAGTAACAAGTATTGGATAGATGTGCAACTTCGATGGGGAGATTATGACTCTCATGATATTGAGCGTTATACTAGGGCCAAATTCATGGACTATACAACTGACAACATGTCTATCTATCCATCTCCAACAGGTGTAATGATTGGGCTTGATCTGGCATACAACCTGCATTCTGCCTTTGGTAATTGGTTCCCTGGATCAAAGCCTCTTCTTGCTCAAGCGATGAACAAGATCATGAAGTCCAATCCGGCTCTATATGTGTTGAGAGAGAGGATAAGGAAAGGTTTGCAGCTATACTCGTCTGAGCCTACAGAGCCATATTTGTCATCTCAAAACTATGGTGAAATATTCAGCAATCAGATTATATGGTTTGTTGATGATACCAATGTTTATCGTGTCACGATTCACAAGACATTTGAAGGAAATTTAACGACAAAGCCAATCAATGGTGCGATTTTCATTTTCAATCCAAGAACTGGGCAGTTGTTCCTGAAGGTTATCCATACAAGTGTTTGGGCTGGTCAGAAGCGTCTTGGCCAGCTAGCGAAATGGAAAACTGCAGAAGAGGTTGCTGCACTTGTGCGATCTCTCCCTGTTGAAGAACAGCCAAAGCAGATTATTGTCACACGGAAAGGAATGTTGGATCCCCTTGAGGTTCACTTGCTTGATTTCCCCAACATTGTTATTAAGGGAAGCGAGCTGCAGCTTCCGTTCCAGGCTTGCCTAAAGATAGAGAAATTTGGTGACCTGATTTTGAAGGCTACAGAACCGCAGATGGTCTTGTTTAATATATATGATGATTGGTTGAAGAGTATTTCTTCTTATACTGCCTTTTCAAGACTTATTTTGATCCTTCGTGCGCTTCACGTGAATAATGAGAAGGCTAAGATGTTGTTGAAGCCTGACAAGTCCGTTGTCACTGAGCCACATCACATCTGGCCCTCTCTCACTGATGATCAATGGATGAAGGTGGAGGTAGCACTTAGAGATCTTATTCTTTCTGACTACGCAAAGAAAAACAATGTGAATACCTCAGCTCTGACACAATCAGAGATCCGAGATATTATTCTTGGAGCTGAGATTACTCCACCCTCTCAACAGAGGCAACAGATAGCTGAGATTGAGAAACAGGCAAAGGAAGCCAGCCAGCTTACTGCAGTCACAACAAGAACTACAAATGTTCATGGTGATGAGCTCATTGTCACCACCACAAGCCCTTACGAGCAATCTGCATTCGGTTCCAAGACAGATTGGCGTGTACGTGCAATATCAGCCACTAATCTTTACCTTAGAGTCAATCACATATATGTGAACTCAGATGACATAAAGGAAACCGGATACACCTACATCATGCCAAAGAACATTTTGAAGAAGTTCATATGCGTTGCGGATCTTCGTACTCAAATTGCTGGGTATTTATATGGTATTAGTCCCCCGGATAATCCCCAGGTGAAGGAGATCCGTTGTGTTGTGATGGTGCCACAGTGGGGAAATCATCAGCTAGTTCACCTGCCGTCATCTCTTCCTGAGCATGATTTCCTAAATGATCTTGAGCCCTTGGGATGGTTGCACACACAGCCTAACGAGCTGCCTCAGCTATCACCACAGGATGTAACCTCTCATTCCCGTATTCTGGAGAACAACAAGCAATGGGATGGAGAAAAATGCATCATCCTGACCTGCAGTTTCACTCCGGGCTCCTGCTCTTTGACGTCATATAAGTTGACCCAAACCGGATATGAATGGGGGCGGCTTAACAAGGACAATGGCAGCAATCCTCACGGTTACCTTCCCACCCATTATGAAAAGGTTCAGATGCTGCTGAGTGACCGGTTCCTCGGGTTTTACATGGTTCCTGAGAGTGGACCGTGGAACTATAGCTTCACTGGAGTGAAGCACACGTTGAGCATGAAATACAGTGTCAAACTCGGATCCCCAAAGGAGTTCTATCACGAGGAGCATCGACCCACTCATTTCCTGGAGTTCAGCAACATGGAGGAGGCTGACATTACAGAGGGAGATCGCGAGGACACATTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2359

Amino Acids

275.42

Weight (kDa)

8.92

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 82 - 233 3.2e-85 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 419 - 824 4.2e-237 PROCN (NUC071) domain
RRM_4 PF10598 1009 - 1099 1e-45 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1261 - 1396 1.7e-75 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1466 - 1624 8.7e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1784 - 2013 1.8e-129 PRP8 domain IV core
JAB PF01398 2124 - 2222 1e-10 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2236 - 2356 5.5e-49 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1851, 2928, 4932
AarI CACCTGC 1 cut(s) 6555
AatII GACGTC 2 cut(s) 2148, 6752
Acc36I ACCTGC 6 cut(s) 397, 1623, 3630, 5211, 6555, 6725
AccB1I GGYRCC 1 cut(s) 6514
AccB7I CCANNNNNTGG 5 cut(s) 1949, 4861, 4873, 5483, 5513
AccI GTMKAC 1 cut(s) 333
AccII CGCG 4 cut(s) 1324, 1329, 2188, 7062
AccIII TCCGGA 1 cut(s) 3651
AcoI YGGCCR 5 cut(s) 1825, 2073, 3470, 4982, 5572
AcuI CTGAAG 3 cut(s) 2627, 3155, 5549
AcvI CACGTG 1 cut(s) 5905
AcyI GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
AdeI CACNNNGTG 1 cut(s) 4085
AflII CTTAAG 1 cut(s) 4778
AflIII ACRYGT 4 cut(s) 328, 879, 5145, 6936
AgeI ACCGGT 1 cut(s) 6867
AhdI GACNNNNNGTC 2 cut(s) 3094, 5146
AjiI CACGTC 2 cut(s) 268, 625
AjuI GAANNNNNNNTTGG 2 cut(s) 5660, 5692
AleI CACNNNNGTG 2 cut(s) 2208, 6926
Alw21I GWGCWC 5 cut(s) 267, 1371, 3924, 4533, 6234
Alw26I GTCTC 8 cut(s) 2596, 3161, 3302, 3484, 3598, 3884, 4441, 4901
AlwNI CAGNNNCTG 3 cut(s) 805, 1268, 6856
Ama87I CYCGRG 3 cut(s) 3237, 3387, 6875
Aor13HI TCCGGA 1 cut(s) 3651
ArsI GACNNNNNNTTYG 4 cut(s) 2701, 2733, 4261, 4293
AseI ATTAAT 2 cut(s) 729, 4698
AsiGI ACCGGT 1 cut(s) 6867
Asp700I GAANNNNTTC 4 cut(s) 1338, 1697, 6410, 6846
AspLEI GCGC 5 cut(s) 97, 1326, 1331, 2188, 5899
AsuC2I CCSGG 3 cut(s) 3896, 6473, 6732
AsuII TTCGAA 1 cut(s) 3012
AsuNHI GCTAGC 1 cut(s) 5578
AvaI CYCGRG 3 cut(s) 3237, 3387, 6875
AxyI CCTNAGG 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 4810
BaeI ACNNNNGTAYC 8 cut(s) 899, 932, 2842, 2875, 3288, 3321, 5409, 5442
BalI TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
BamHI GGATCC 4 cut(s) 628, 1099, 5680, 6968
BanI GGYRCC 1 cut(s) 6514
BanII GRGCYC 6 cut(s) 346, 1963, 3924, 6234, 6594, 6737
BarI GAAGNNNNNNTAC 6 cut(s) 2347, 2379, 3639, 3671, 3891, 3923
BauI CACGAG 2 cut(s) 4148, 6989
BbrPI CACGTG 1 cut(s) 5905
BbsI GAAGAC 2 cut(s) 3520, 3542
Bbv12I GWGCWC 5 cut(s) 267, 1371, 3924, 4533, 6234
BbvCI CCTCAGC 2 cut(s) 6070, 6630
BceAI ACGGC 5 cut(s) 108, 223, 1695, 4177, 6535
BcgI CGANNNNNNTGC 8 cut(s) 1094, 1128, 1469, 1503, 1541, 1575, 6248, 6282
BciVI GTATCC 5 cut(s) 2360, 3289, 3443, 3648, 6368
BclI TGATCA 3 cut(s) 1077, 2836, 5992
BcnI CCSGG 3 cut(s) 3896, 6473, 6732
BcoDI GTCTC 8 cut(s) 2596, 3161, 3302, 3484, 3598, 3884, 4441, 4901
BfaI CTAG 8 cut(s) 599, 656, 2810, 3996, 4998, 5112, 5579, 6539
BfoI RGCGCY 1 cut(s) 98
BfrI CTTAAG 1 cut(s) 4778
BfuAI ACCTGC 6 cut(s) 397, 1623, 3630, 5211, 6555, 6725
BfuI GTATCC 5 cut(s) 2360, 3289, 3443, 3648, 6368
BglII AGATCT 2 cut(s) 4291, 6025
BlpI GCTNAGC 1 cut(s) 156
BmeRI GACNNNNNGTC 2 cut(s) 3094, 5146
BmeT110I CYCGRG 3 cut(s) 3237, 3387, 6875
BmgBI CACGTC 2 cut(s) 268, 625
BmrI ACTGGG 2 cut(s) 732, 5523
BmtI GCTAGC 1 cut(s) 5582
BmuI ACTGGG 2 cut(s) 732, 5523
BoxI GACNNNNGTC 1 cut(s) 2865
BpiI GAAGAC 2 cut(s) 3520, 3542
BpmI CTGGAG 7 cut(s) 656, 1362, 2138, 2460, 6692, 6945, 7037
Bpu10I CCTNAGC 5 cut(s) 28, 261, 6070, 6564, 6630
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 3012
BpuEI CTTGAG 7 cut(s) 182, 1625, 2618, 4547, 5238, 5708, 6608
BpuMI CCSGG 3 cut(s) 3896, 6473, 6732
Bsa29I ATCGAT 1 cut(s) 3033
BsaAI YACGTR 2 cut(s) 5905, 6299
BsaBI GATNNNNATC 3 cut(s) 1104, 2829, 3510
BsaHI GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
BsaI GGTCTC 2 cut(s) 3161, 3884
BsaWI WCCGGW 9 cut(s) 124, 440, 744, 1387, 1927, 3651, 6371, 6770, 6867
BsaXI ACNNNNNCTCC 2 cut(s) 4574, 4604
Bse118I RCCGGY 1 cut(s) 6867
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 6692
Bse8I GATNNNNATC 3 cut(s) 1104, 2829, 3510
BseAI TCCGGA 1 cut(s) 3651
BseCI ATCGAT 1 cut(s) 3033
BseJI GATNNNNATC 3 cut(s) 1104, 2829, 3510
BseMI GCAATG 1 cut(s) 6692
BseRI GAGGAG 7 cut(s) 24, 111, 179, 602, 4616, 7007, 7049
BseSI GKGCMC 1 cut(s) 4810
BseYI CCCAGC 3 cut(s) 3254, 4045, 6446
BsgI GTGCAG 2 cut(s) 4751, 5595
Bsh1236I CGCG 4 cut(s) 1324, 1329, 2188, 7062
Bsh1285I CGRYCG 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 6514
BshTI ACCGGT 1 cut(s) 6867
BshVI ATCGAT 1 cut(s) 3033
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 5 cut(s) 267, 1371, 3924, 4533, 6234
BsiHKCI CYCGRG 3 cut(s) 3237, 3387, 6875
BsmAI GTCTC 8 cut(s) 2596, 3161, 3302, 3484, 3598, 3884, 4441, 4901
BsmI GAATGC 3 cut(s) 567, 5206, 6269
Bso31I GGTCTC 2 cut(s) 3161, 3884
BsoBI CYCGRG 3 cut(s) 3237, 3387, 6875
Bsp119I TTCGAA 1 cut(s) 3012
Bsp13I TCCGGA 1 cut(s) 3651
Bsp1407I TGTACA 2 cut(s) 3907, 4897
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 2 cut(s) 470, 2243
Bsp68I TCGCGA 1 cut(s) 7062
BspDI ATCGAT 1 cut(s) 3033
BspEI TCCGGA 1 cut(s) 3651
BspFNI CGCG 4 cut(s) 1324, 1329, 2188, 7062
BspHI TCATGA 4 cut(s) 2692, 3882, 5092, 5271
BspMAI CTGCAG 6 cut(s) 1806, 3751, 5599, 5742, 6196, 6722
BspMI ACCTGC 6 cut(s) 397, 1623, 3630, 5211, 6555, 6725
BspOI GCTAGC 1 cut(s) 5582
BspQI GCTCTTC 1 cut(s) 2649
BspT104I TTCGAA 1 cut(s) 3012
BspT107I GGYRCC 1 cut(s) 6514
BspTI CTTAAG 1 cut(s) 4778
BspTNI GGTCTC 2 cut(s) 3161, 3884
BsrDI GCAATG 1 cut(s) 6692
BsrFI RCCGGY 1 cut(s) 6867
BsrGI TGTACA 2 cut(s) 3907, 4897
BssAI RCCGGY 1 cut(s) 6867
BssNAI GTATAC 1 cut(s) 334
BssNI GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
BssSI CACGAG 2 cut(s) 4148, 6989
BssT1I CCWWGG 6 cut(s) 337, 470, 1344, 2243, 4919, 6594
Bst1107I GTATAC 1 cut(s) 334
Bst2BI CACGAG 2 cut(s) 4148, 6989
BstACI GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
BstAFI CTTAAG 1 cut(s) 4778
BstAPI GCANNNNNTGC 1 cut(s) 4238
BstAUI TGTACA 2 cut(s) 3907, 4897
BstBAI YACGTR 2 cut(s) 5905, 6299
BstBI TTCGAA 1 cut(s) 3012
BstDSI CCRYGG 4 cut(s) 470, 2243, 4193, 6905
BstEII GGTNACC 3 cut(s) 2259, 5780, 6821
BstENI CCTNNNNNAGG 1 cut(s) 2210
BstFNI CGCG 4 cut(s) 1324, 1329, 2188, 7062
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 5 cut(s) 97, 1326, 1331, 2188, 5899
BstMAI GTCTC 8 cut(s) 2596, 3161, 3302, 3484, 3598, 3884, 4441, 4901
BstMCI CGRYCG 1 cut(s) 129
BstNSI RCATGY 6 cut(s) 256, 391, 850, 883, 3637, 5149
BstPAI GACNNNNGTC 1 cut(s) 2865
BstPI GGTNACC 3 cut(s) 2259, 5780, 6821
BstSLI GKGCMC 1 cut(s) 4810
BstUI CGCG 4 cut(s) 1324, 1329, 2188, 7062
BstV2I GAAGAC 2 cut(s) 3520, 3542
BstXI CCANNNNNNTGG 3 cut(s) 4434, 4505, 5125
BstZ17I GTATAC 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 3033
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 5 cut(s) 2360, 3289, 3443, 3648, 6368
BsuTUI ATCGAT 1 cut(s) 3033
BtgI CCRYGG 4 cut(s) 470, 2243, 4193, 6905
BtgZI GCGATG 1 cut(s) 5273
BtrI CACGTC 2 cut(s) 268, 625
BtsI GCAGTG 1 cut(s) 2990
BtuMI TCGCGA 1 cut(s) 7062
BveI ACCTGC 6 cut(s) 397, 1623, 3630, 5211, 6555, 6725
CaiI CAGNNNCTG 3 cut(s) 805, 1268, 6856
CciI TCATGA 4 cut(s) 2692, 3882, 5092, 5271
CfoI GCGC 5 cut(s) 97, 1326, 1331, 2188, 5899
Cfr10I RCCGGY 1 cut(s) 6867
ClaI ATCGAT 1 cut(s) 3033
CpoI CGGWCCG 1 cut(s) 4389
CseI GACGC 7 cut(s) 1330, 2045, 2855, 4265, 4578, 4886, 5555
CsiI ACCWGGT 1 cut(s) 889
CspAI ACCGGT 1 cut(s) 6867
CspCI CAANNNNNGTGG 4 cut(s) 2052, 2087, 2828, 2863
CspI CGGWCCG 1 cut(s) 4389
DraI TTTAAA 4 cut(s) 1608, 1893, 2743, 3367
DraIII CACNNNGTG 1 cut(s) 4085
DriI GACNNNNNGTC 2 cut(s) 3094, 5146
EaeI YGGCCR 5 cut(s) 1825, 2073, 3470, 4982, 5572
Eam1105I GACNNNNNGTC 2 cut(s) 3094, 5146
EciI GGCGGA 3 cut(s) 90, 1712, 3088
Ecl136II GAGCTC 2 cut(s) 3922, 6232
Eco130I CCWWGG 6 cut(s) 337, 470, 1344, 2243, 4919, 6594
Eco24I GRGCYC 6 cut(s) 346, 1963, 3924, 6234, 6594, 6737
Eco31I GGTCTC 2 cut(s) 3161, 3884
Eco32I GATATC 2 cut(s) 3407, 4785
Eco53kI GAGCTC 2 cut(s) 3922, 6232
Eco57I CTGAAG 3 cut(s) 2627, 3155, 5549
Eco72I CACGTG 1 cut(s) 5905
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 3 cut(s) 3237, 3387, 6875
Eco91I GGTNACC 3 cut(s) 2259, 5780, 6821
EcoICRI GAGCTC 2 cut(s) 3922, 6232
EcoNI CCTNNNNNAGG 1 cut(s) 2210
EcoO109I RGGNCCY 1 cut(s) 2251
EcoO65I GGTNACC 3 cut(s) 2259, 5780, 6821
EcoRI GAATTC 1 cut(s) 4317
EcoRV GATATC 2 cut(s) 3407, 4785
EcoT14I CCWWGG 6 cut(s) 337, 470, 1344, 2243, 4919, 6594
EcoT22I ATGCAT 4 cut(s) 2142, 2830, 4761, 6707
EcoT38I GRGCYC 6 cut(s) 346, 1963, 3924, 6234, 6594, 6737
ErhI CCWWGG 6 cut(s) 337, 470, 1344, 2243, 4919, 6594
FalI AAGNNNNNCTT 8 cut(s) 1466, 1498, 2472, 2504, 2855, 2887, 4146, 4178
FauI CCCGC 4 cut(s) 41, 79, 3756, 4215
FauNDI CATATG 3 cut(s) 2963, 4366, 6416
FbaI TGATCA 3 cut(s) 1077, 2836, 5992
FblI GTMKAC 1 cut(s) 333
FriOI GRGCYC 6 cut(s) 346, 1963, 3924, 6234, 6594, 6737
FspBI CTAG 8 cut(s) 599, 656, 2810, 3996, 4998, 5112, 5579, 6539
GlaI GCGC 5 cut(s) 96, 1325, 1330, 2187, 5898
GsaI CCCAGC 3 cut(s) 3258, 4049, 6450
GsuI CTGGAG 7 cut(s) 656, 1362, 2138, 2460, 6692, 6945, 7037
HaeII RGCGCY 1 cut(s) 98
HgaI GACGC 7 cut(s) 1330, 2045, 2855, 4265, 4578, 4886, 5555
HhaI GCGC 5 cut(s) 97, 1326, 1331, 2188, 5899
Hin1I GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
Hin6I GCGC 5 cut(s) 95, 1324, 1329, 2186, 5897
HinP1I GCGC 5 cut(s) 95, 1324, 1329, 2186, 5897
HincII GTYRAC 6 cut(s) 1833, 2406, 3769, 3853, 4626, 6762
HindII GTYRAC 6 cut(s) 1833, 2406, 3769, 3853, 4626, 6762
HindIII AAGCTT 3 cut(s) 158, 2676, 3684
Hpy99I CGWCG 1 cut(s) 549
Hsp92I GRCGYC 5 cut(s) 2056, 2145, 4276, 4589, 6749
HspAI GCGC 5 cut(s) 95, 1324, 1329, 2186, 5897
Kpn2I TCCGGA 1 cut(s) 3651
Ksp22I TGATCA 3 cut(s) 1077, 2836, 5992
LguI GCTCTTC 1 cut(s) 2649
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 8 cut(s) 599, 656, 2810, 3996, 4998, 5112, 5579, 6539
MfeI CAATTG 2 cut(s) 647, 1787
MlsI TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
MluNI TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
MmeI TCCRAC 7 cut(s) 140, 1786, 4358, 4716, 4854, 5189, 5658
Mox20I TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
Mph1103I ATGCAT 4 cut(s) 2142, 2830, 4761, 6707
MroI TCCGGA 1 cut(s) 3651
MroXI GAANNNNTTC 4 cut(s) 1338, 1697, 6410, 6846
MscI TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
Msp20I TGGCCA 5 cut(s) 1827, 2075, 3472, 4984, 5574
MspA1I CMGCKG 3 cut(s) 74, 1265, 4222
MspCI CTTAAG 1 cut(s) 4778
MteI GCGCNGCGC 1 cut(s) 1327
MunI CAATTG 2 cut(s) 647, 1787
Mva1269I GAATGC 3 cut(s) 567, 5206, 6269
MvnI CGCG 4 cut(s) 1324, 1329, 2188, 7062
NciI CCSGG 3 cut(s) 3896, 6473, 6732
NcoI CCATGG 2 cut(s) 470, 2243
NdeI CATATG 3 cut(s) 2963, 4366, 6416
NheI GCTAGC 1 cut(s) 5578
NruI TCGCGA 1 cut(s) 7062
NsiI ATGCAT 4 cut(s) 2142, 2830, 4761, 6707
NspI RCATGY 6 cut(s) 256, 391, 850, 883, 3637, 5149
NspV TTCGAA 1 cut(s) 3012
OliI CACNNNNGTG 2 cut(s) 2208, 6926
PaeR7I CTCGAG 1 cut(s) 3237
PagI TCATGA 4 cut(s) 2692, 3882, 5092, 5271
PaqCI CACCTGC 1 cut(s) 6555
PasI CCCWGGG 2 cut(s) 399, 2255
PciI ACATGT 2 cut(s) 879, 5145
PciSI GCTCTTC 1 cut(s) 2649
PcsI WCGNNNNNNNCGW 1 cut(s) 2713
PctI GAATGC 3 cut(s) 567, 5206, 6269
PdmI GAANNNNTTC 4 cut(s) 1338, 1697, 6410, 6846
PflMI CCANNNNNTGG 5 cut(s) 1949, 4861, 4873, 5483, 5513
PfoI TCCNGGA 2 cut(s) 3894, 7014
PinAI ACCGGT 1 cut(s) 6867
PmaCI CACGTG 1 cut(s) 5905
PmlI CACGTG 1 cut(s) 5905
Ppu21I YACGTR 2 cut(s) 5905, 6299
PpuMI RGGWCCY 1 cut(s) 2251
PscI ACATGT 2 cut(s) 879, 5145
PshAI GACNNNNGTC 1 cut(s) 2865
PshBI ATTAAT 2 cut(s) 729, 4698
PsiI TTATAA 3 cut(s) 1851, 2928, 4932
Psp124BI GAGCTC 2 cut(s) 3924, 6234
Psp5II RGGWCCY 1 cut(s) 2251
PspCI CACGTG 1 cut(s) 5905
PspEI GGTNACC 3 cut(s) 2259, 5780, 6821
PspFI CCCAGC 3 cut(s) 3254, 4045, 6446
PspPPI RGGWCCY 1 cut(s) 2251
PspXI VCTCGAGB 1 cut(s) 3237
PstI CTGCAG 6 cut(s) 1806, 3751, 5599, 5742, 6196, 6722
PstNI CAGNNNCTG 3 cut(s) 805, 1268, 6856
PvuII CAGCTG 1 cut(s) 1265
RruI TCGCGA 1 cut(s) 7062
Rsr2I CGGWCCG 1 cut(s) 4389
RsrII CGGWCCG 1 cut(s) 4389
SacI GAGCTC 2 cut(s) 3924, 6234
SapI GCTCTTC 1 cut(s) 2649
SexAI ACCWGGT 1 cut(s) 889
Sfr274I CTCGAG 1 cut(s) 3237
SfuI TTCGAA 1 cut(s) 3012
SlaI CTCGAG 1 cut(s) 3237
SmlI CTYRAG 9 cut(s) 161, 1640, 2597, 3237, 4526, 4778, 5253, 5687, 6587
SmoI CTYRAG 9 cut(s) 161, 1640, 2597, 3237, 4526, 4778, 5253, 5687, 6587
SspI AATATT 3 cut(s) 3082, 4752, 5385
SspMI CTAG 8 cut(s) 599, 656, 2810, 3996, 4998, 5112, 5579, 6539
SstI GAGCTC 2 cut(s) 3924, 6234
StyI CCWWGG 6 cut(s) 337, 470, 1344, 2243, 4919, 6594
TaqII GACCGA 2 cut(s) 1109, 4406
TatI WGTACW 3 cut(s) 1002, 3907, 4897
TauI GCSGC 5 cut(s) 64, 106, 1329, 2911, 6790
TspGWI ACGGA 9 cut(s) 305, 1119, 2454, 3428, 4793, 5680, 5736, 5938, 6488
Van91I CCANNNNNTGG 5 cut(s) 1949, 4861, 4873, 5483, 5513
Vha464I CTTAAG 1 cut(s) 4778
VspI ATTAAT 2 cut(s) 729, 4698
XagI CCTNNNNNAGG 1 cut(s) 2210
XbaI TCTAGA 1 cut(s) 2809
XceI RCATGY 6 cut(s) 256, 391, 850, 883, 3637, 5149
XcmI CCANNNNNNNNNTGG 7 cut(s) 1763, 1793, 3109, 4133, 4451, 5970, 7012
XhoI CTCGAG 1 cut(s) 3237
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 4 cut(s) 1338, 1697, 6410, 6846
XspI CTAG 8 cut(s) 599, 656, 2810, 3996, 4998, 5112, 5579, 6539
ZraI GACGTC 2 cut(s) 2146, 6750
Zsp2I ATGCAT 4 cut(s) 2142, 2830, 4761, 6707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.