Rh1BG154600

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000798
Physical Location & Seq
Forward (+)
25311361 .. 25311864
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG154600.1

Sequence Viewer

Length: 504 bp
ATGCAGAAGTCGAATACAGCTTTGCAATCAAGGAAGTCAGCTTTCCAAGTAATGAAGTCAAACCCAGGTTTCACTCTCTTGGCTTTGCAGATGCGAGACTTCATGTTAAACCCAGCTCTGAACGTCTTGAGTGAGTACATAAAGAAAGGACTGTTGGAGTCGTCCTCTTCGGAGCCTACGGAACTGTTCTTGTCATCCAAAAACTATGCTGAGATTTTCTTCTATCCAATAAGGACTTGGTTTGTGCACGAGGCAGCCAATGTGTATCCAGTTACAATCAATGGAACTCCATTTAGTCGTGCTATTTTCACATTCAATCCGTGCACCGAGCAGCTTCTCTTGAAGACCAATATCCATGCTGCTAACACAAGACAAGAGGTAGCTGCGCTTTTGCATTCAGTTCCAGTTGGGGAACATCCAAAGAGAATCTTTGTGAACATGTGTAAGGGAATGCATGATCATCCACCGAAAGGGTTTCCCTACCATAGTCATGAAAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

167

Amino Acids

19.01

Weight (kDa)

9.45

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP8_domainIV PF12134 61 - 153 3.1e-08 PRP8 domain IV core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 2 cut(s) 316, 343
AjnI CCWGG 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 5 cut(s) 20, 41, 116, 334, 383
AluI AGCT 5 cut(s) 20, 41, 116, 334, 383
Alw21I GWGCWC 2 cut(s) 249, 326
Alw26I GTCTC 1 cut(s) 90
Alw44I GTGCAC 2 cut(s) 245, 322
ApaLI GTGCAC 2 cut(s) 245, 322
ApeKI GCWGC 4 cut(s) 254, 331, 359, 383
AspLEI GCGC 1 cut(s) 388
BaeGI GKGCMC 2 cut(s) 249, 326
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 2 cut(s) 249, 326
BbvI GCAGC 4 cut(s) 266, 343, 346, 370
BciT130I CCWGG 1 cut(s) 66
BciVI GTATCC 1 cut(s) 276
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 90
BfuI GTATCC 1 cut(s) 276
BisI GCNGC 4 cut(s) 255, 332, 360, 384
BlsI GCNGC 4 cut(s) 256, 333, 361, 385
Bme1390I CCNGG 1 cut(s) 66
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpuEI CTTGAG 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 269, 404
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 3 cut(s) 194, 415, 460
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 269, 404
BseSI GKGCMC 2 cut(s) 249, 326
BseXI GCAGC 4 cut(s) 266, 343, 346, 370
BseYI CCCAGC 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 249, 326
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 1 cut(s) 90
BsmI GAATGC 2 cut(s) 394, 456
Bsp1286I GDGCHC 2 cut(s) 249, 326
Bsp143I GATC 1 cut(s) 457
BspCNI CTCAG 1 cut(s) 202
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 174
BsrI ACTGG 2 cut(s) 269, 404
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 1 cut(s) 457
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 153, 186
Bst6I CTCTTC 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 194, 415, 460
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 1 cut(s) 457
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 64
BstSLI GKGCMC 2 cut(s) 249, 326
BstV1I GCAGC 4 cut(s) 266, 343, 346, 370
BstV2I GAAGAC 1 cut(s) 350
BsuI GTATCC 1 cut(s) 276
BtsCI GGATG 3 cut(s) 194, 415, 460
CciI TCATGA 1 cut(s) 490
CfoI GCGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 136
CviAII CATG 5 cut(s) 103, 356, 439, 455, 491
CviJI RGCY 8 cut(s) 20, 41, 83, 116, 175, 257, 334, 383
CviKI_1 RGCY 8 cut(s) 20, 41, 83, 116, 175, 257, 334, 383
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 210
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 456
FaeI CATG 5 cut(s) 106, 359, 442, 458, 494
FaiI YATR 8 cut(s) 104, 140, 207, 357, 440, 456, 486, 492
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FatI CATG 5 cut(s) 102, 355, 438, 454, 490
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 255, 332, 360, 384
FokI GGATG 3 cut(s) 181, 402, 447
Fsp4HI GCNGC 4 cut(s) 255, 332, 360, 384
GlaI GCGC 1 cut(s) 387
GluI GCNGC 4 cut(s) 255, 332, 360, 384
GsaI CCCAGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 388
Hin1II CATG 5 cut(s) 106, 359, 442, 458, 494
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HinfI GANTC 2 cut(s) 158, 426
Hpy166II GTNNAC 3 cut(s) 247, 324, 436
Hpy188I TCNGA 2 cut(s) 120, 172
Hpy188III TCNNGA 3 cut(s) 127, 340, 491
Hpy8I GTNNAC 3 cut(s) 247, 324, 436
HpyCH4III ACNGT 2 cut(s) 153, 186
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 7 cut(s) 4, 25, 88, 247, 324, 394, 454
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 5 cut(s) 106, 359, 442, 458, 494
HspAI GCGC 1 cut(s) 386
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 1 cut(s) 172
LpnPI CCDG 5 cut(s) 51, 78, 126, 282, 417
Lsp1109I GCAGC 4 cut(s) 266, 343, 346, 370
LweI GCATC 1 cut(s) 81
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 271
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 3 cut(s) 159, 211, 355
MhlI GDGCHC 2 cut(s) 249, 326
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 3 cut(s) 175, 244, 370
Mph1103I ATGCAT 1 cut(s) 456
MseI TTAA 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 66
Mva1269I GAATGC 2 cut(s) 394, 456
MvaI CCWGG 1 cut(s) 66
NdeII GATC 1 cut(s) 457
NlaIII CATG 5 cut(s) 106, 359, 442, 458, 494
NlaIV GGNNCC 1 cut(s) 174
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 442
PagI TCATGA 1 cut(s) 490
PciI ACATGT 1 cut(s) 438
PctI GAATGC 2 cut(s) 394, 456
PfeI GAWTC 1 cut(s) 426
PkrI GCNGC 4 cut(s) 256, 333, 361, 385
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PscI ACATGT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 174
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 4 cut(s) 255, 332, 360, 384
Sau3AI GATC 1 cut(s) 457
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 2 cut(s) 249, 326
SetI ASST 8 cut(s) 22, 43, 70, 118, 126, 336, 381, 385
SfaNI GCATC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 153, 186
TaiI ACGT 1 cut(s) 126
TaqI TCGA 1 cut(s) 11
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TseI GCWGC 4 cut(s) 254, 331, 359, 383
TspDTI ATGAA 2 cut(s) 68, 91
TspGWI ACGGA 2 cut(s) 194, 309
VneI GTGCAC 2 cut(s) 245, 322
XceI RCATGY 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.