Rw2G030580

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000798
Physical Location & Seq
Reverse (-)
50052070 .. 50061602
9533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030580.1

Sequence Viewer

Length: 6984 bp
ATGTGGAACAACGGCCAGATTGTGCCGCCAGGCACTGGTGGCTCGTCGATTCCGCCACCACCCGCGGCTCAGCCCTCTTATACGGTGTTGCCGCCTCCGGCAGATGCCGAGGCCGTGCTCGAAGAGAAGGCTCGTAAATGGCAGCAACTCAATTCCAAGCGGTACAGCGACAAGCGCAAGTTTGGCTTCGTTGAGACGCAGAAGGAGGACATGCCTCCTGAGCATGTCAGGAAGATCATTAGAGACCATGGGGACATGTCCTCGAAGAAATTCCGTCACGATAAACGCGTGTATCTAGGAGCACTTAAATTTGTTCCGCATGCAGTTTACAAGCTTCTCGAGAATATGCCAATGCCCTGGGAGCAGGTCCGTGATGTGAAGGTTCTGTACCATATAACTGGGGCAATTACATTTTGGGGTTCAATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGACATTTTAAAAGAATGCGGTTTCCACCTTTTGATGATGAGGAACCTCCATTAGATTATGCTGATAATCTGTTAGATGTTGATCCTCTAGAGCCAATTCAACTGGAGTTGGATGAAGACGAAGATTCTGCTGTTTATACTTGGTTTTATGACCATAAACCTCTAGTCAAGACAAAACTCATTAACGGTCCAAGTTACCGGAAATGGCATCTCTCCCTTCCAATCATGGCAACTCTCCACCGGCTTGCAGGACAGCTACTATCTGATTTGATAGACCGCAATTATTTCTACTTGTTTGACATGGAGTCTTTCTTTACAGCGAAAGCCCTCAATATGTGCATTCCAGGTGGGCCTAAGTTTGAACCTTTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATTAACAAGCTGATTATTCGGTCACCTCTCAGAACAGAGTACAGAATTGCGTTCCCTCATCTCTACAACAATAGACCCAGGAAGGTAAAGCTCTGTGTGTATCACACACCCATGGTTATGTACATAAAAACTGAGGATCCTGATCTACCTGCATTTTATTATGATCCCTTGATACACCCAATCCCCAGCACAAACAAGGAGCGACGTGAAAAGAAGATTGATGAGGATGATGACACTTTCATACTGCCAGAAGGGGTAGAACCATTTCTGAGTGATACTCAGCTTTATACTGACACAACAGCAGCTGGAGTTTCCCTCTTGTTTGCACCACGCCCCTTTAACATGAGATCTGGGCGGACTCGCCGTGCAGAAGATATACCCCTTGTGTCAGAGTGGTATAAAGAGCATTGTCCTCCGTCCTATCCAGTGAAAGTGCGGGTCAGCTATCAGAAATTGTTAAAATGTTTTGTGTTGAACGAGTTGCATCATAGACCACCCAAGGCTCAAAAGAAGAAGCACTTGTTTCGATCACTTCAAGCTACTAAGTTCTTCCAAACTACAGAACTTGATTGGGCTGAAGCGGGTCTTCAAGTCTGCAAGCAGGGTTATAACATGCTAAATCTCTTGATCCACAGGAAAAATTTGAATTACCTTCACCTTGATTATAATTTCAATCTGAAGCCTGTGAAAACCCTGACAACTAAAGAGCGTAAAAAGTCCCGGTTTGGTAATGCTTTTCATCTTTGCCGTGAGATCTTGCGGTTGACAAAGCTGGTAGTTGATGCCAACATCCAGTTCCGTTTGGGTAATGTCGATGCTTTTCAATTGGCTGATGGCCTGCAGTACACTTTCTCTCATGTTGGTCAGTTGACTGGTATGTATCGTTACAAATACAGGTTGATGCGTCAGATTAGAATGTGCAAAGATCTGAAGCACTTGATTTACTACCGTTTCAACACGGGACCAGTTGGGAAAGGACCTGGATGTGGATTCTGGGCGCCGATGTGGAGGGTTTGGTTGTTCTTCCTTCGTGGCATAGTTCCTCTTTTGGAACGGTGGTTGGGAAATTTACTTGCACGGCAATTTGAAGGGCGCCATTCAAAGGGAGTGGCAAAAACTGTAACTAAGCAGCGTGTGGAAAGCCATTTTGATTTGGAGCTTCGAGCTGCTGTCATGCATGATGTTCTTGATGCGATGCCTGAGGGAATCAAGCAAAATAAGGCCAGAACTATATTGCAACATCTCAGTGAGGCATGGCGTTGCTGGAAAGCAAATATTCCTTGGAAGGTACCTGGTTTGCCAGTTCCCATTGAGAATATGATTCTTCGTTATGTCAAGTCAAAGGCAGACTGGTGGACAAATGTGGCTCATTACAATCGTGAACGTATAAGAAGAGGTGCTACAGTAGATAAGACAGTTTGTCGGAAGAATCTTGGAAGATTAACACGCCTTTGGCTGAAGGCAGAGCAGGAACGACAACACAATTATCTCAAAGATGGCCCATATGTTACACCAGAGGAGGCAGTTGCAATTTACACGACAACTGTGCATTGGTTGGAGTCAAGAAAGTTCAGCCCCATCCCATTTCCTCCATTGTCGTACAAGCATGATACCAAGCTACTCATCCTTGCTCTGGAGAGGTTGAAGGAATCATATAGTGTTGCTGTGAGATTGAATCAGCTTCAAAGAGAAGAGCTTGGTCTTATTGAACAAGCCTATGATAACCCGCATGAAGCATTGTCACGAATTAAGCGCCACTTACTCACTCAGCGTGCCTTCAAAGAAGTTGGCATAGAGTTCATGGATTTGTACAGCTATCTCATTCCAGTCTATGAGATTGAACCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGGTGACAAACGTCATCTCTTCCCGAACTGGATCAAGCCTGCAGATTCGGAGCCACCTCCACTCTTGGTTTATAAATGGTGTCAGGGAATAAACAATTTACAGAGTATCTGGGACACGAGTGACGGGCAGTGTGTTGTCATGCTTCAGACCAAGTTTGAGAAGTTCTTTGAAAAGATTGACTTGACAATGCTAAATAGGCTTCTACGTCTAGTTCTTGATCATAATATCGCTGATTATGTCACTGCGAAGAACAATGTGGTATTGTCCTACAAGGATATGAGTCATACAAATTCGTATGGTCTTATTCGTGGTCTTCAGTTTGCATCCTTTGTTGTACAGTATTATGGGCTTGTGTTGGATCTATTGCTTCTTGGATTGACTCGAGCTAGTGAGATTGCAGGTCCACCCCAGATGCCAAATGAGTTCATCACTTATTGGGACACAAAGGTCGAGACCCGGCATCCTATCAGGCTGTACTCTCGATACATAGACAGGGTGCACATCTTGTTCCGCTTCACTCATGAGGAAGCTCGGGATCTTATCCAGCGATATCTCACTGAACATCCTGATCCTAATAATGAGAATATGGTGGGATATAACAACAAGAAGTGCTGGCCAAGAGATGCCAGAATGAGGCTCATGAAACACGATGTCAATCTTGGAAGGAGTGTTTTCTGGGATATGAAAAACCGTCTCCCTCGAAGCATCACAACTTTAGAGTGGGAGAACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTCTTCAGCATGTGTGGATTTGAGGTTCGGATACTTCCCAAGATAAGAATGAGCCAAGAGGCATTCAGCAACACAAGAGATGGAGTTTGGAATTTGCAGAATGAACAGACAAAGGAAAGGACTGCAGTTGCTTTCTTACGTGTTGATGATGAACACATGAAAGTATTTGAGAATCGTGTGAGGCAGATCCTTATGTCGTCAGGGTCTACCACATTTACAAAGATTGTCAACAAATGGAATACAGCTCTGATTGGTCTTATGACTTACTTCCGGGAAGCAACTGTACATACGCAAGAACTGTTAGATTTACTAGTCAAGTGCGAAAACAAAATTCAGACCCGTATTAAGATTGGATTAAATTCAAAGATGCCTAGTAGGTTTCCACCTGTCATCTTTTACACACCAAAAGAAATTGGAGGACTTGGAATGTTATCTATGGGTCACATATTGATTCCCCAAAGTGATCTCAGATACAGTCAGCAGACAGATGTTGGTGTGACACACTTTAGGAGTGGGATGAAGGACCAGCTGATTCCAAATCTGTACCGTTACATACAACCTTGGGAGAGTGAATTCATTGATTCCCAGCGGGTTTGGGCAGAATATGCTCTGAAAAGGCAGGAGGCTCAAGCACAAAACAGGCGCCTAACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATTCCACGCATAAATACATTGTTCCAGAAAGATCGGCACACTTTAGCATATGACAAAGGGTGGAGAGTTCGTACTGATTTTAAGCAGTACCAGGTCTTGAAACAAAATCCTTTTTGGTGGACACACCAAAGGCATGACGGAAAACTTTGGAACTTGAACAACTACAGAACTGATGTTATTCAAGCTCTTGGAGGTGTGGAGGGAATCTTGGAGCATACCTTATTTAAAGGAACATACTTCCCTACCTGGGAGGGTCTCTTCTGGGAAAAAGCATCTGGTTTTGAAGAATCGATGAAGTACAAGAAGCTGACTAATGCACAGAGATCTGGGCTCAACCAAATTCCCAATCGTAGGTTCACGCTTTGGTGGTCGCCGACCATTAATCGGGCTAATGTATATGTCGGTTTCCAAGTGCAGTTAGATCTTACTGGGATCTTTATGCATGGGAAAATTCCAACCTTGAAGATATCCTTGATTCAAATATTCCGCGCACATTTGTGGCAAAAGATCCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAGGAGTTAGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACGATTCATCCCAGGAAGAGTTACAAAATGAACAGTTCTTGTGCTGACATTCTACTCTTTGCTGCCCATAGATGGCCAATGTCAAAACCTAGTCTTGTGGCGGAGCCAAAGGATGTGTTTGATCAGAAAGCAAGCAACAAGTACTGGATAGATGTACAGCTCCGATGGGGAGATTATGATTCCCACGATATTGAACGTTACACCCGAGCCAAGTTTATGGATTATACAACAGACAACATGTCTATCTATCCCTCCCCCACAGGGGTGATGATTGGTCTTGATCTGGCGTATAATTTACATTCTGCTTTTGGTAATTGGTTTCCTGGGTCCAAACCACTGCTTCAGCAGGCAATGAACAAAATAATGAAGTCAAATCCAGCTTTGTACGTCTTGCGGGAGCGGATACGGAAAGGATTGCAGCTTTACTCTTCAGAGCCTACAGAACCTTACTTGTCATCCCAGAACTATGGGGAGATTTTCAGCAATCAAATTATTTGGTTTGTTGATGATACTAATGTGTATCGTGTTACAATCCACAAGACATTTGAAGGCAATCTCACTACAAAACCAATTAATGGTGCGATTTTCATATTCAATCCAAGGACTGGGCAGCTGTTCCTGAAGGTCATCCACACAAGTGTATGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCCAAGTGGAAAACCGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTACCAGTTGAAGAGCAGCCAAAACAAATTATTGTGACTCGAAAGGGAATGCTTGATCCGCTGGAGGTGCACTTGTTGGATTTCCCTAACATAGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTCCAGGCTTGTCTTAAGATTGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAGCCACAGATGGTTCTTTTCAACATCTATGATGATTGGTTGAAGAGTATATCATCATATACTGCATTCTCTCGGCTCATTTTGATTCTTCGTGCACTTCATGTGAACAATGAGAAGGCAAAGATGTTACTGAAACCTGATACAACAGTCATTACTGAGCCACACCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTAAGGGATCTGATTCTCTCAGATTATGCAAAAAAGAACAACGTGAACACATCGGCTCTGACACAATCTGAGATTCGTGATATCATTCTTGGAGCTGAGATTACTCCTCCTTCACAACAAAGGCAACAAATTGCAGAGATTGAAAAGCAGCATAAAGAAGCAAGTCAGCTGACAGCAGTTACTACTAGGACCACGAATGTGCATGGTGATGAACTCATTGTCACCACTACTAGTCCTTATGAGCAAGCTGCATTTGGGTCCAAGACTGATTGGCGTGTGCGAGCAATATCGGCTACCAATCTCTACCTTCGTGTCAATCATATATATGTGAATTCAGAGGATGTGAAGGAAACGGGTTACACTTATATCATGCCAAAGAACATATTGAAGAAGTTCATCTGTGTTGCGGATCTGAGGACACAAATTGCTGGTTACTTGTACGGTGTAAGCCCACCTGACAACCCTCAGGTTAAGGAAATTCGCTGCATTGCCATGCCACCGCAGTGGGGAACCCACCAGCAAGTTAATCTTCCAACGGCTCTTCCCGAGCATGATTTCCTCAATGATCTGGAACCTTTGGGATGGATGCACACACAACCAAACGAACTTCCTCAGCTTTCTCCTCAGGATCTAACCTCTCATGCTAAGATCTTGGAAAGCACCAAGCAATGGGATGGGGAGAAGTGCATCATTTTAACCTGCAGTTTCACTCCTGGTTCGTGCTCGTTAACTGCATACAAACTCACTCCATCTGGTTATGAGTGGGGACGGGTCAACAAAGACACAGGAAGCAATCCACACGGTTACCTCCCAACTCATTACGAGAAGGTCCAAATGCTTCTCAGTGATCGGTTCCTTGGTTTCTATATGATTCCTGATAATGGTCCGTGGAACTACAACTTCATGGGGGTGAAGCATACACAAGGCATGAAATATGGTATCAAGCTTGGGACACCGAGGGAGTACTATCATGAGGACCACCGGCCAACCCATTACCTCGAGTTTAGCAACATGGAGGAGGGTGACACAGTGGTGGGAGATCGTGACGATATATTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2327

Amino Acids

271.85

Weight (kDa)

8.98

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 204 8.3e-69 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 390 - 795 1.1e-235 PROCN (NUC071) domain
RRM_4 PF10598 980 - 1070 1.4e-46 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1232 - 1364 1.6e-70 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1434 - 1592 8.6e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1752 - 1981 2.6e-129 PRP8 domain IV core
JAB PF01398 2092 - 2191 1.1e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2204 - 2324 2.3e-48 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1485, 1542, 2841
AasI GACNNNNNNGTC 2 cut(s) 3215, 5469
Acc36I ACCTGC 4 cut(s) 355, 1033, 3158, 6629
Acc65I GGTACC 1 cut(s) 2164
AccB1I GGYRCC 4 cut(s) 1873, 1968, 2164, 4179
AccB7I CCANNNNNTGG 4 cut(s) 35, 5387, 5417, 6591
AccBSI CCGCTC 1 cut(s) 5212
AccI GTMKAC 2 cut(s) 460, 3743
AccII CGCG 3 cut(s) 65, 288, 4710
AclI AACGTT 1 cut(s) 5008
AcoI YGGCCR 5 cut(s) 13, 3383, 4886, 5484, 6905
AcyI GRCGYC 3 cut(s) 1874, 1969, 4180
AflII CTTAAG 1 cut(s) 5666
AflIII ACRYGT 4 cut(s) 255, 286, 3676, 5049
AhdI GACNNNNNGTC 3 cut(s) 763, 2295, 5050
AhlI ACTAGT 2 cut(s) 3847, 6152
AjiI CACGTC 1 cut(s) 1082
AjuI GAANNNNNNNTTGG 4 cut(s) 1655, 1687, 4559, 4591
AleI CACNNNNGTG 6 cut(s) 4717, 5075, 5448, 6119, 6424, 6953
AloI GAACNNNNNNTCC 2 cut(s) 3546, 3578
Alw21I GWGCWC 6 cut(s) 120, 304, 3270, 5601, 5803, 6647
Alw26I GTCTC 6 cut(s) 188, 237, 3215, 3467, 4481, 4790
Alw44I GTGCAC 3 cut(s) 3266, 5597, 5799
AlwNI CAGNNNCTG 2 cut(s) 35, 1181
Ama87I CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
ApaLI GTGCAC 3 cut(s) 3266, 5597, 5799
AseI ATTAAT 2 cut(s) 4602, 5385
Asp700I GAANNNNTTC 4 cut(s) 269, 1140, 4815, 6314
Asp718I GGTACC 1 cut(s) 2164
AspLEI GCGC 6 cut(s) 177, 1876, 1971, 2631, 4182, 4712
AsuC2I CCSGG 3 cut(s) 1597, 3226, 3809
AvaI CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
AxyI CCTNAGG 3 cut(s) 2076, 6387, 6546
BaeGI GKGCMC 3 cut(s) 3270, 5601, 5803
BaeI ACNNNNGTAYC 8 cut(s) 275, 308, 812, 845, 5313, 5346, 5838, 5871
BalI TGGCCA 3 cut(s) 3385, 4888, 5486
BamHI GGATCC 1 cut(s) 1012
BanI GGYRCC 4 cut(s) 1873, 1968, 2164, 4179
BanII GRGCYC 1 cut(s) 4554
BarI GAAGNNNNNNTAC 6 cut(s) 371, 403, 2260, 2292, 3804, 3836
BauI CACGAG 1 cut(s) 2884
BbsI GAAGAC 3 cut(s) 582, 1454, 3074
Bbv12I GWGCWC 6 cut(s) 120, 304, 3270, 5601, 5803, 6647
BbvCI CCTCAGC 1 cut(s) 6534
BceAI ACGGC 6 cut(s) 28, 98, 1224, 1608, 1970, 6474
BcgI CGANNNNNNTGC 6 cut(s) 569, 603, 1382, 1416, 2404, 2438
BciVI GTATCC 2 cut(s) 3561, 5208
BclI TGATCA 4 cut(s) 2749, 2986, 4933, 5896
BcnI CCSGG 3 cut(s) 1597, 3226, 3809
BcoDI GTCTC 6 cut(s) 188, 237, 3215, 3467, 4481, 4790
BcuI ACTAGT 2 cut(s) 3847, 6152
BfmI CTRYAG 9 cut(s) 1434, 1715, 2277, 2808, 3660, 4384, 5250, 5642, 6622
BfoI RGCGCY 4 cut(s) 1877, 1972, 2632, 4183
BfrI CTTAAG 1 cut(s) 5666
BfuAI ACCTGC 4 cut(s) 355, 1033, 3158, 6629
BfuI GTATCC 2 cut(s) 3561, 5208
BglII AGATCT 6 cut(s) 1223, 1629, 1801, 4544, 4642, 6570
BlpI GCTNAGC 1 cut(s) 69
BmcAI AGTACT 2 cut(s) 4955, 6887
BmeRI GACNNNNNGTC 3 cut(s) 763, 2295, 5050
BmeT110I CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
BmgBI CACGTC 1 cut(s) 1082
BmrI ACTGGG 3 cut(s) 408, 4659, 5427
BmuI ACTGGG 3 cut(s) 408, 4659, 5427
BoxI GACNNNNGTC 1 cut(s) 2778
BpiI GAAGAC 3 cut(s) 582, 1454, 3074
BplI GAGNNNNNCTC 4 cut(s) 1138, 1170, 1176, 1208
BpmI CTGGAG 4 cut(s) 584, 1203, 2531, 5612
Bpu10I CCTNAGC 2 cut(s) 219, 6534
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 2744, 4149
BpuMI CCSGG 3 cut(s) 1597, 3226, 3809
Bsa29I ATCGAT 1 cut(s) 4511
BsaAI YACGTR 1 cut(s) 3677
BsaBI GATNNNNATC 5 cut(s) 540, 1017, 2742, 2754, 3423
BsaHI GRCGYC 3 cut(s) 1874, 1969, 4180
BsaI GGTCTC 3 cut(s) 237, 3215, 4481
BsaWI WCCGGW 1 cut(s) 657
BsaXI ACNNNNNCTCC 2 cut(s) 4276, 4306
Bse118I RCCGGY 2 cut(s) 699, 6903
Bse21I CCTNAGG 3 cut(s) 2076, 6387, 6546
Bse3DI GCAATG 3 cut(s) 5169, 6408, 6596
Bse8I GATNNNNATC 5 cut(s) 540, 1017, 2742, 2754, 3423
BseCI ATCGAT 1 cut(s) 4511
BseJI GATNNNNATC 5 cut(s) 540, 1017, 2742, 2754, 3423
BseMI GCAATG 3 cut(s) 5169, 6408, 6596
BseRI GAGGAG 4 cut(s) 2408, 6018, 6534, 6953
BseSI GKGCMC 3 cut(s) 3270, 5601, 5803
BseYI CCCAGC 2 cut(s) 1061, 4122
BsgI GTGCAG 4 cut(s) 1263, 4655, 4823, 5499
Bsh1236I CGCG 3 cut(s) 65, 288, 4710
BshNI GGYRCC 4 cut(s) 1873, 1968, 2164, 4179
BshVI ATCGAT 1 cut(s) 4511
BsiHKAI GWGCWC 6 cut(s) 120, 304, 3270, 5601, 5803, 6647
BsiHKCI CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
BsiSI CCGG 7 cut(s) 98, 658, 700, 1597, 3226, 3808, 6904
BslFI GGGAC 7 cut(s) 266, 1579, 1851, 2894, 3221, 6702, 6886
BsmAI GTCTC 6 cut(s) 188, 237, 3215, 3467, 4481, 4790
BsmBI CGTCTC 2 cut(s) 188, 3467
BsmFI GGGAC 7 cut(s) 266, 1579, 1851, 2894, 3221, 6702, 6886
BsmI GAATGC 5 cut(s) 480, 798, 3599, 5583, 5771
Bso31I GGTCTC 3 cut(s) 237, 3215, 4481
BsoBI CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
Bsp1286I GDGCHC 7 cut(s) 120, 304, 3270, 4554, 5601, 5803, 6647
Bsp1407I TGTACA 5 cut(s) 996, 2685, 3103, 3820, 4966
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 2 cut(s) 247, 987
BspDI ATCGAT 1 cut(s) 4511
BspFNI CGCG 3 cut(s) 65, 288, 4710
BspHI TCATGA 3 cut(s) 3289, 3408, 6892
BspMAI CTGCAG 5 cut(s) 1719, 2812, 3664, 5646, 6626
BspMI ACCTGC 4 cut(s) 355, 1033, 3158, 6629
BspQI GCTCTTC 4 cut(s) 2562, 3545, 5535, 6468
BspT107I GGYRCC 4 cut(s) 1873, 1968, 2164, 4179
BspTI CTTAAG 1 cut(s) 5666
BspTNI GGTCTC 3 cut(s) 237, 3215, 4481
BsrBI CCGCTC 1 cut(s) 5212
BsrDI GCAATG 3 cut(s) 5169, 6408, 6596
BsrFI RCCGGY 2 cut(s) 699, 6903
BsrGI TGTACA 5 cut(s) 996, 2685, 3103, 3820, 4966
BssAI RCCGGY 2 cut(s) 699, 6903
BssNI GRCGYC 3 cut(s) 1874, 1969, 4180
BssSI CACGAG 1 cut(s) 2884
BssT1I CCWWGG 7 cut(s) 247, 987, 1374, 2156, 4097, 5411, 6778
Bst2BI CACGAG 1 cut(s) 2884
BstACI GRCGYC 3 cut(s) 1874, 1969, 4180
BstAFI CTTAAG 1 cut(s) 5666
BstAPI GCANNNNNTGC 1 cut(s) 4142
BstAUI TGTACA 5 cut(s) 996, 2685, 3103, 3820, 4966
BstBAI YACGTR 1 cut(s) 3677
BstDSI CCRYGG 4 cut(s) 63, 247, 987, 6809
BstEII GGTNACC 2 cut(s) 897, 6725
BstFNI CGCG 3 cut(s) 65, 288, 4710
BstH2I RGCGCY 4 cut(s) 1877, 1972, 2632, 4183
BstHHI GCGC 6 cut(s) 177, 1876, 1971, 2631, 4182, 4712
BstMAI GTCTC 6 cut(s) 188, 237, 3215, 3467, 4481, 4790
BstNSI RCATGY 7 cut(s) 214, 227, 259, 323, 1492, 3550, 5053
BstPAI GACNNNNGTC 1 cut(s) 2778
BstPI GGTNACC 2 cut(s) 897, 6725
BstSFI CTRYAG 9 cut(s) 1434, 1715, 2277, 2808, 3660, 4384, 5250, 5642, 6622
BstSLI GKGCMC 3 cut(s) 3270, 5601, 5803
BstUI CGCG 3 cut(s) 65, 288, 4710
BstV2I GAAGAC 3 cut(s) 582, 1454, 3074
BstXI CCANNNNNNTGG 5 cut(s) 357, 398, 5029, 5279, 6426
Bsu15I ATCGAT 1 cut(s) 4511
Bsu36I CCTNAGG 3 cut(s) 2076, 6387, 6546
BsuI GTATCC 2 cut(s) 3561, 5208
BsuTUI ATCGAT 1 cut(s) 4511
BtgI CCRYGG 4 cut(s) 63, 247, 987, 6809
BtgZI GCGATG 1 cut(s) 2084
BtrI CACGTC 1 cut(s) 1082
BtsI GCAGTG 4 cut(s) 2903, 3009, 5147, 6431
BveI ACCTGC 4 cut(s) 355, 1033, 3158, 6629
CaiI CAGNNNCTG 2 cut(s) 35, 1181
CciI TCATGA 3 cut(s) 3289, 3408, 6892
CfoI GCGC 6 cut(s) 177, 1876, 1971, 2631, 4182, 4712
Cfr10I RCCGGY 2 cut(s) 699, 6903
Cfr42I CCGCGG 1 cut(s) 66
ClaI ATCGAT 1 cut(s) 4511
CseI GACGC 3 cut(s) 205, 1769, 5459
CsiI ACCWGGT 2 cut(s) 2167, 4311
CspCI CAANNNNNGTGG 2 cut(s) 1965, 2000
DinI GGCGCC 3 cut(s) 1875, 1970, 4181
DraI TTTAAA 2 cut(s) 469, 4447
DrdI GACNNNNNNGTC 2 cut(s) 3215, 5469
DriI GACNNNNNGTC 3 cut(s) 763, 2295, 5050
DseDI GACNNNNNNGTC 2 cut(s) 3215, 5469
EaeI YGGCCR 5 cut(s) 13, 3383, 4886, 5484, 6905
Eam1105I GACNNNNNGTC 3 cut(s) 763, 2295, 5050
EciI GGCGGA 3 cut(s) 42, 1246, 4928
Eco130I CCWWGG 7 cut(s) 247, 987, 1374, 2156, 4097, 5411, 6778
Eco24I GRGCYC 1 cut(s) 4554
Eco31I GGTCTC 3 cut(s) 237, 3215, 4481
Eco32I GATATC 3 cut(s) 3320, 4689, 6004
Eco81I CCTNAGG 3 cut(s) 2076, 6387, 6546
Eco88I CYCGRG 6 cut(s) 338, 3150, 3300, 5016, 6467, 6920
Eco91I GGTNACC 2 cut(s) 897, 6725
EcoO109I RGGNCCY 1 cut(s) 1853
EcoO65I GGTNACC 2 cut(s) 897, 6725
EcoRI GAATTC 3 cut(s) 4109, 4221, 6253
EcoRV GATATC 3 cut(s) 3320, 4689, 6004
EcoT14I CCWWGG 7 cut(s) 247, 987, 1374, 2156, 4097, 5411, 6778
EcoT22I ATGCAT 3 cut(s) 2055, 2743, 4665
EcoT38I GRGCYC 1 cut(s) 4554
EgeI GGCGCC 3 cut(s) 1875, 1970, 4181
EheI GGCGCC 3 cut(s) 1875, 1970, 4181
ErhI CCWWGG 7 cut(s) 247, 987, 1374, 2156, 4097, 5411, 6778
Esp3I CGTCTC 2 cut(s) 188, 3467
FaqI GGGAC 7 cut(s) 266, 1579, 1851, 2894, 3221, 6702, 6886
FauI CCCGC 6 cut(s) 70, 1305, 1450, 2610, 4119, 5199
FauNDI CATATG 2 cut(s) 2380, 4270
FbaI TGATCA 4 cut(s) 2749, 2986, 4933, 5896
FblI GTMKAC 2 cut(s) 460, 3743
FriOI GRGCYC 1 cut(s) 4554
GlaI GCGC 6 cut(s) 176, 1875, 1970, 2630, 4181, 4711
GsaI CCCAGC 2 cut(s) 1065, 4126
GsuI CTGGAG 4 cut(s) 584, 1203, 2531, 5612
HaeII RGCGCY 4 cut(s) 1877, 1972, 2632, 4183
HapII CCGG 7 cut(s) 98, 658, 700, 1597, 3226, 3808, 6904
HgaI GACGC 3 cut(s) 205, 1769, 5459
HhaI GCGC 6 cut(s) 177, 1876, 1971, 2631, 4182, 4712
Hin1I GRCGYC 3 cut(s) 1874, 1969, 4180
Hin6I GCGC 6 cut(s) 175, 1874, 1969, 2629, 4180, 4710
HinP1I GCGC 6 cut(s) 175, 1874, 1969, 2629, 4180, 4710
HincII GTYRAC 6 cut(s) 461, 1641, 1746, 3766, 6651, 6697
HindII GTYRAC 6 cut(s) 461, 1641, 1746, 3766, 6651, 6697
HindIII AAGCTT 2 cut(s) 332, 6866
HpaI GTTAAC 1 cut(s) 6651
HpaII CCGG 7 cut(s) 98, 658, 700, 1597, 3226, 3808, 6904
Hpy99I CGWCG 3 cut(s) 49, 462, 1083
HpyCH4IV ACGT 8 cut(s) 1081, 2260, 2779, 2974, 3676, 5008, 5199, 5964
HpySE526I ACGT 8 cut(s) 1081, 2260, 2779, 2974, 3676, 5008, 5199, 5964
Hsp92I GRCGYC 3 cut(s) 1874, 1969, 4180
HspAI GCGC 6 cut(s) 175, 1874, 1969, 2629, 4180, 4710
KasI GGCGCC 3 cut(s) 1873, 1968, 4179
KpnI GGTACC 1 cut(s) 2168
Ksp22I TGATCA 4 cut(s) 2749, 2986, 4933, 5896
KspAI GTTAAC 1 cut(s) 6651
KspI CCGCGG 1 cut(s) 66
LguI GCTCTTC 4 cut(s) 2562, 3545, 5535, 6468
MabI ACCWGGT 2 cut(s) 2167, 4311
MaeII ACGT 8 cut(s) 1081, 2260, 2779, 2974, 3676, 5008, 5199, 5964
MbiI CCGCTC 1 cut(s) 5212
MfeI CAATTG 1 cut(s) 1700
MhlI GDGCHC 7 cut(s) 120, 304, 3270, 4554, 5601, 5803, 6647
MlsI TGGCCA 3 cut(s) 3385, 4888, 5486
MluI ACGCGT 1 cut(s) 286
MluNI TGGCCA 3 cut(s) 3385, 4888, 5486
Mly113I GGCGCC 3 cut(s) 1874, 1969, 4180
MlyI GAGTC 6 cut(s) 773, 1228, 2444, 3058, 3142, 5560
MmeI TCCRAC 7 cut(s) 549, 2279, 2412, 3105, 4700, 5586, 6479
Mox20I TGGCCA 3 cut(s) 3385, 4888, 5486
Mph1103I ATGCAT 3 cut(s) 2055, 2743, 4665
MroXI GAANNNNTTC 4 cut(s) 269, 1140, 4815, 6314
MscI TGGCCA 3 cut(s) 3385, 4888, 5486
Msp20I TGGCCA 3 cut(s) 3385, 4888, 5486
MspA1I CMGCKG 7 cut(s) 65, 1181, 4066, 4126, 5425, 5590, 6091
MspCI CTTAAG 1 cut(s) 5666
MspI CCGG 7 cut(s) 98, 658, 700, 1597, 3226, 3808, 6904
MunI CAATTG 1 cut(s) 1700
Mva1269I GAATGC 5 cut(s) 480, 798, 3599, 5583, 5771
MvnI CGCG 3 cut(s) 65, 288, 4710
NarI GGCGCC 3 cut(s) 1874, 1969, 4180
NciI CCSGG 3 cut(s) 1597, 3226, 3809
NcoI CCATGG 2 cut(s) 247, 987
NdeI CATATG 2 cut(s) 2380, 4270
NmeAIII GCCGAG 2 cut(s) 133, 5758
NsiI ATGCAT 3 cut(s) 2055, 2743, 4665
NspI RCATGY 7 cut(s) 214, 227, 259, 323, 1492, 3550, 5053
OliI CACNNNNGTG 6 cut(s) 4717, 5075, 5448, 6119, 6424, 6953
PaeI GCATGC 1 cut(s) 323
PaeR7I CTCGAG 3 cut(s) 338, 3150, 6920
PagI TCATGA 3 cut(s) 3289, 3408, 6892
PasI CCCWGGG 1 cut(s) 357
PciI ACATGT 2 cut(s) 255, 5049
PciSI GCTCTTC 4 cut(s) 2562, 3545, 5535, 6468
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 5014
PctI GAATGC 5 cut(s) 480, 798, 3599, 5583, 5771
PdmI GAANNNNTTC 4 cut(s) 269, 1140, 4815, 6314
PflMI CCANNNNNTGG 4 cut(s) 35, 5387, 5417, 6591
PfoI TCCNGGA 1 cut(s) 3807
PleI GAGTC 6 cut(s) 772, 1228, 2443, 3057, 3142, 5560
PluTI GGCGCC 3 cut(s) 1877, 1972, 4183
PpsI GAGTC 6 cut(s) 772, 1228, 2443, 3057, 3142, 5560
Ppu21I YACGTR 1 cut(s) 3677
PpuMI RGGWCCY 1 cut(s) 1853
PscI ACATGT 2 cut(s) 255, 5049
PshAI GACNNNNGTC 1 cut(s) 2778
PshBI ATTAAT 2 cut(s) 4602, 5385
PsiI TTATAA 3 cut(s) 1485, 1542, 2841
Psp1406I AACGTT 1 cut(s) 5008
Psp5II RGGWCCY 1 cut(s) 1853
PspEI GGTNACC 2 cut(s) 897, 6725
PspFI CCCAGC 2 cut(s) 1061, 4122
PspPPI RGGWCCY 1 cut(s) 1853
PspXI VCTCGAGB 2 cut(s) 3150, 6920
PstI CTGCAG 5 cut(s) 1719, 2812, 3664, 5646, 6626
PstNI CAGNNNCTG 2 cut(s) 35, 1181
PvuII CAGCTG 4 cut(s) 1181, 4066, 5425, 6091
SacII CCGCGG 1 cut(s) 66
SalI GTCGAC 1 cut(s) 459
SapI GCTCTTC 4 cut(s) 2562, 3545, 5535, 6468
ScaI AGTACT 2 cut(s) 4955, 6887
SchI GAGTC 6 cut(s) 773, 1228, 2444, 3058, 3142, 5560
SduI GDGCHC 7 cut(s) 120, 304, 3270, 4554, 5601, 5803, 6647
SexAI ACCWGGT 2 cut(s) 2167, 4311
SfcI CTRYAG 9 cut(s) 1434, 1715, 2277, 2808, 3660, 4384, 5250, 5642, 6622
SfoI GGCGCC 3 cut(s) 1875, 1970, 4181
Sfr274I CTCGAG 3 cut(s) 338, 3150, 6920
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SlaI CTCGAG 3 cut(s) 338, 3150, 6920
SmlI CTYRAG 6 cut(s) 338, 2723, 3150, 4164, 5666, 6920
SmoI CTYRAG 6 cut(s) 338, 2723, 3150, 4164, 5666, 6920
SpeI ACTAGT 2 cut(s) 3847, 6152
SphI GCATGC 1 cut(s) 323
SspDI GGCGCC 3 cut(s) 1873, 1968, 4179
SspI AATATT 2 cut(s) 2152, 4704
StyI CCWWGG 7 cut(s) 247, 987, 1374, 2156, 4097, 5411, 6778
TaiI ACGT 8 cut(s) 1084, 2263, 2782, 2977, 3679, 5011, 5202, 5967
TaqII GACCGA 1 cut(s) 885
TauI GCSGC 3 cut(s) 28, 68, 94
TspGWI ACGGA 7 cut(s) 263, 359, 1281, 1664, 4374, 5233, 6798
Van91I CCANNNNNTGG 4 cut(s) 35, 5387, 5417, 6591
Vha464I CTTAAG 1 cut(s) 5666
VneI GTGCAC 3 cut(s) 3266, 5597, 5799
VspI ATTAAT 2 cut(s) 4602, 5385
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 7 cut(s) 214, 227, 259, 323, 1492, 3550, 5053
XcmI CCANNNNNNNNNTGG 1 cut(s) 5874
XhoI CTCGAG 3 cut(s) 338, 3150, 6920
XmiI GTMKAC 2 cut(s) 460, 3743
XmnI GAANNNNTTC 4 cut(s) 269, 1140, 4815, 6314
ZrmI AGTACT 2 cut(s) 4955, 6887
Zsp2I ATGCAT 3 cut(s) 2055, 2743, 4665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.