MD17G1212800.v1.1

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000798
Physical Location & Seq
Forward (+)
25997411 .. 26008279
10869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1212800.v1.1.491

Sequence Viewer

Length: 7044 bp
ATGTGGAACAACGGCCAGATTGCGCCGCCTGGCACCGGCGGTTCGTCTATTCCGCCACCGCCGGCGGCTCAGCCATCTTACACGGTGTTGCCTTCTCCAGCTGATGCCGAGGCTAAGCTTGAAGAGAAGGCCCGGAAATGGCAGCAGCTTAATTCCAAGCGGTATAGCGATAAGCGCAAGTTCGGCTTCGTCGAGTCGCAGAAGGAGGACATGCCTCCTGAACATGTCAGGAAGATCATTAGGGACCATGGAGATATGTCCTCAAAGAAATTTCGTCATGATAAACGAGTGTATCTCGGAGCACTTAAGTTTGTCCCCCATGCCGTTTACAAGCTCCTTGAGAACATGCCAATGCCATGGGAGCAGGTCCGGGATGTGAAGGTTTTGTACCATATAACTGGGGCAATTACATTTGTAAATGAAATACCTTGGGTTGTTGAACCCATCTATTTGGCTCAGTGGGGTTCAATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGGCATTTCAAAAGAATGCGGTTTCCACCATTTGATGATGAGGAACCTCCGTTAGACTATGCTGATAATCTGTTAGACGTTGATCCCCTAGAGCCAATTCAATTGGAGTTGGATGAAGAAGAAGATTCTGCTGTGTATACATGGTTTTATGACCATAAACCTCTTGTTAAGACAAAGCTCATAAACGGCCCAAGTTATAGGAAGTGGCATCTGTCGCTTCCCATCATGGCAACTCTTCATCGACTAGCTGGACAACTGCTTTCTGATCTTATTGACCGCAATTATTTCTACCTTTTCGACATGGAGTCTTTTTTTACAGCCAAAGCACTCAATATGTGCATTCCAGGTGGACCTAAATTTGAACCATTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATCAACAAACTCATTATTCGGTCACCTCTCAGAACGGAGTACAGGATTGCATTCCCTCATCTCTACAACAATAGACCCAGGAAGGTCAAGCTTTGTGTGTATCACACACCCATGGTGATGTACATAAAAACCGAGGATCCTGATCTACCTGCGTTTTATTATGATCCGTTGATACACCCAATACCCAGCAGTAATAAGGATAGGCGTGAGAAGAAAACTTCTGATGAGGAGGAAGATGATATCTTTACATTGCCAGATGTGGTGGAACCATTTCTCAACGATACTCAGCTTTATACTGACACCACAGCTGCCGGAATCTCTCTTTTGTTTGCCCCACGCCCTTTTAACATGAGATCTGGTCGGACCCGTCGAGCAGAAGATATACCCCTTGTGTCTGAGTGGTACAAGGAACATTGTCCTCCTTCATATCCAGTTAAAGTTCGGGTCAGCTATCAGAAATTGCTGAAGTGTTTTGTATTGAACGAGCTGCATCATAGACCACCCAAAGCTCAAAAGAAGAAACATTTGTTTCGATCTCTTCAAGCTACCAAGTTCTTCCAAACTACAGAACTTGATTGGGCTGAAGCGGGTCTTCAAGTCTGTAAGCAGGGTTATAACATGCTTAATCTGTTGATCCACAGGAAAAATTTGAATTACCTTCACCTTGATTATAATTTCAATCTGAAGCCTGTGAAGACTTTGACAACAAAAGAGCGTAAGAAATCTCGGTTTGGTAATGCTTTTCATCTGTGCCGTGAAATCTTGCGGTTGACAAAGCTTGTAGTTGATGCCAATATCCAGTTCCGTTTGGGTAATGTTGATGCCTTTCAATTGGCTGATGGCCTACAATACACGTTTTCTCATGTTGGTCAGTTGACTGGAATGTACCGTTACAAGTATAGGTTGATGAGGCAGATTAGAATGTGCAAAGATTTGAAGCACTTGATTTACTACCGGTTCAATACTGGGCCTGTGGGAAAAGGACCAGGATGTGGGTTCTGGGCACCAATGTGGAGGGTGTGGTTGTTCTTCCTTCGTGGCATAGTACCTCTTCTGGAACGGTGGCTGGGAAATTTACTTGCACGACAGTTTGAAGGGCGCCATTCAAAAGGAGTGGCAAAGACTGTTACTAAGCAGCGTGTTGAAAGCCATTTCGACTTGGAGCTTCGAGCTGCTGTGATGCATGATGTTCTTGATGCCATGCCAGAGGGTATCAAGCAAAATAAAGCCAGAACTATCTTGCAACATCTCAGTGAGGCATGGCGCTGCTGGAAAGCTAACATCCCTTGGAAGGTTCCTGGTTTGCCTGTTCCTATTGAAAATATGATTCTTCGTTATGTGAAATCGAAGGCAGACTGGTGGACAAATGTTGCTCACTACAATCGTGAACGTATAAGAAGAGGTGCTACTGTAGATAAGACAGTTTGTCGGAAGAATCTAGGAAGATTAACTCGTCTCTGGCTAAAGGCAGAGCAGGAGCGTCAGCACAATTATTTGAAAGATGGTCCATATGTTACACCAGAAGAGGCAGTTGCAATTTACACCACAACCGTGCACTGGTTGGAATCAAGAAAGTTTACACCCATTCCGTTCCCTCCATTGTCATACAAACATGACACCAAGCTTCTGATCCTTGCTCTGGAGAGGTTAAAGGAGTCTTATAGTGTGGCTGTAAGATTGAACCAGCTTCAAAGAGAAGAGTTGGGTCTCATTGAACAAGCCTATGACAATCCTCACGAGGCATTGTCACGAATTAAGCGCCACTTGCTCACTCAGCGTGCATTCAAAGAAGTTGGTATAGAGTTCATGGATTTGTATAGCTATCTGATTCCAGTTTATGAGATAGAACCTCTTGAGAAGATTACGGATGCATATCTTGATCAATATTTATGGTATGAGGGTGACAAACGTCATCTCTTTCCAAACTGGATTAAGCCTGCAGATTCAGAGCCACCTCCACTCTTGGTTTATAAATGGTGTCAGGGAATAAACAATTTACAGAGCATATGGGACACGAGTGAGGGGCAGTGTGTGGTGATGCTTCAAACGAAGTTTGAGAAGTTTTTTGAAAAGATTGACTTGACAATGCTAAATAGGCTCCTGCGTCTTGTTCTTGACCATAATATTGCTGATTATGTCACTGCAAAGAACAATGTGGTACTGTCTTACAAAGATATGAGTCATACAAATTCGTATGGTCTCATACGTGGTCTTCAGTTTGCATCTTTTGTAGTGCAATATTATGGGCTTGTGTTGGATCTGTTACTTCTTGGGTTGACTCGGGCCAGTGAAATTGCCGGTCCACCCCAGATGCCAAATGAATTCATCACCTATTGGGACACAAAGGTTGAGACACGGCATCCTATCAGGCTGTATTCTAGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACCCACGAGGAGGCTCGTGATCTTATTCAGCGATATCTTACTGAGCATCCGGACCCCAATAATGAAAATATGGTTGGATACAATAATAAGAAATGCTGGCCAAGAGATGCCAGAATGAGGCTCATGAAACATGATGTCAATCTTGGGAGGAGTGTTTTCTGGGATATGAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAGTGGGAGAACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTTTTCAGCATGTGTGGATTTGAAGTTCGGATACTTCCCAAGATAAGAATGAGTCAAGAAGCATTCAGCAACACTAGGGATGGGGTTTGGAATTTGCAGAATGAGCAGACAAAGGAGAGGACTGCAGTTGCTTTCTTACGTGTTGATGATGAACACATGAAGGTGTTTGAGAATCGTGTGAGGCAGATTCTTATGTCCTCAGGGTCTACAACTTTTACAAAAATTGTCAACAAGTGGAATACAGCGCTTATTGGTCTAATGACTTACTTCCGTGAAGCAACTGTTCATACACAAGAACTGTTGGATTTGCTTGTCAAATGTGAAAATAAGATTCAGACCCGTATTAAGATTGGGTTGAATTCAAAGATGCCTAGCAGGTTTCCACCTGTCATCTTTTACACACCAAAGGAAATTGGAGGGCTTGGCATGTTATCTATGGGGCACATATTGATTCCACAAAGTGACCTCCGCTTCAGTCAGCAGACTGATGTTGGTGTGAGTCATTTTAGGAGTGGAATGAGTCATGAAGGGGACCAGCTGATTCCAAATCTTTACCGTTACATACAACCATGGGAGAGTGAATTTATAGATTCACAACGTGTTTGGGCAGAATATGCACTGAAAAGGCAGGAAGCTCAGGCACAAAATAGGCGTCTGACTCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACTTTGTTCCAAAAGGATCGGCATACTTTAGCATATGACAAAGGTTGGAGAGTTCGGACAGATTTTAAGCAGTACCAAGTGTTGAAACAAAATCCTTTCTGGTGGACACACCAGAGGCATGATGGTAAATTGTGGAACTTAAACAACTACCGAACTGATGTGATTCAAGCCCTTGGAGGTGTTGAAGGAATCCTTGAGCATACGTTATTCAAAGGAACATACTTTCCTACCTGGGAGGGGCTCTTCTGGGAGAAAGCATCTGGTTTTGAGGAGTCGATGAAGTATAAAAAGTTGACAAATGCACAGAGATCTGGGCTCAATCAAATCCCCAATCGTAGGTTTACTCTCTGGTGGTCACCTACCATAAATCGGGCCAATGTATATGTTGGTTTCCAAGTGCAGCTAGATTTAACTGGAATATTTATGCACGGGAAGATTCCAACCTTGAAGATATCACTGATTCAGATATTCCGTGCTCACTTGTGGCAGAAGATTCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAAGAGTTGGATGCTTTAGAAATTGAAACTGTGCAGAAGGAAACGATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCTGACATTCTTCTGTTTGCTGCCCATAGATGGCCAATGTCGAAACCTAGTCTTGTTGCTGAGCCAAAGGATGTGTTTGACCAGAAAGCGAGCAATAAGTACTGGATAGATGTACAACTCCGTTGGGGAGATTATGATTCTCACGATATTGAGCGTTACACCCGAGCTAAGTTTATGGACTATACCACTGACAACATGTCCATCTATCCATCTCCCACAGGGGTGATGATTGGTCTTGATTTGGCATATAATTTGCATTCTGCATTTGGTAACTGGTTTCCTGGTTCAAAACCGCTGCTTCAACAGGCAATGAACAAGATCATGAAATCAAATCCAGCCTTGTATGTCCTGAGGGAGCGAATAAGGAAAGGATTGCAGCTATATTCTTCCGAGCCTACAGAGCCATATTTGTCATCCCAAAACTATGGGGAGATCTTCAGCAATCAAATCATTTGGTTTGTCGATGATACCAATGTGTATCGTGTTACAATCCATAAGACATTTGAAGGAAATCTCACTACGAAACCAATCAATGGTGCTATCTTCATATTCAATCCAAGGACGGGGCAGCTATTCCTGAAGGTTATCCACACAAGTGTGTGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCTAAATGGAAAACTGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTGCCAGTGGAAGAACAGCCAAAACAAATTATTGTGACTCGCAAGGGAATGTTGGATCCCTTAGAGGTTCATTTGCTCGATTTCCCCAACATAGTCATAAAAGGAAGTGAGCTCCAGCTTCCTTTCCAAGCGTGTTTGAAGATCGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAACCACAGATGGTTCTTTTCAATATTTATGATGATTGGTTGAAGAGTATTTCATCATACACTGCATTCTCTCGGTTAATTTTGATTCTTCGTGCACTTCATGTGAACAATGAGAAGGCAAAGATGTTACTGAAGCCTGATAAAACGGTCATTACCGAGCCACATCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTAAGGGATCTCATATTGTCAGACTACGCAAAAAAGAACAATGTGAATACATCAGCCCTAACACAATCTGAGATCCGTGACATTATACTCGGAGCTGAGATCACCCCACCTTCTCAACAGAGACAGCAAATTGCAGAAATAGAAAAACAGGCCAAAGAAGCCAGTCAGCTGACAGCAGTCACAACAAGGACTACAAATGTGCATGGTGATGAACTCATTGTCACCACTACAAGTCCCTATGAGCAAAGTGCATTTGGCTCCAAAACTGATTGGCGTGTGAGAGCAATATCAGCTACAAATCTATATCTTCGTGTCAATCATATATATGTGAATTCAGAAGATATAAAGGAAACGGGTTACACCTATATTATGCCTAAGAACATTTTGAAAAAGTTCATCTGCATTGCGGATCTGAGGACGCAAATAGCTGGTTATTTGTATGGCATAAGCCCACCGGACAACCCTCAGGTCAAGGAAATCCGCTGCATTGCAATGCCACCACAATGGGGGACCCACCAACAAGTTCATCTTCCATCAGCTCTTCCTGATCATGATTTCCTCAACGATTTGGAGCCTCTGGGATGGATGCACACCCAGCCAAATGAGCTTCCTCAGCTTTCTCCTCAGGATCTCACTTCTCATGCCAAGATTTTAGAGAACACCAAGCAATGGGATGGGGAGAAATGCATCATTTTAACCTGCAGTTTCACTCCAGGTTCCTGCTCTTTAACCGCATACAAACTTACTCCATCTGGTTATGAATGGGGACGTGTCAATAAAGACACAGGAAGCAATCCTCATGGATACCTTCCCACTCATTACGAGAAGGTTCAGATGCTTCTCAGTGACCGCTTCCTTGGTTTCTATATGATTCCGGACACCGGTCCATGGAACTACAACTTCATGGGGGTGAAGCATACACCTAGCATGAAGTACGGCATCAAGCTTGGAACGCCCAGGGAGTACTATCACGAGGACCACAGGCCGACCCATTACCTTGAGTTCAGCAACCTGGAGGAGGGTGACACGGTGGAAGGAGATCGCGATGACACTTTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2348

Amino Acids

274.16

Weight (kDa)

8.91

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 219 2.7e-85 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 407 - 812 6.2e-236 PROCN (NUC071) domain
RRM_4 PF10598 997 - 1087 1.4e-46 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1249 - 1384 1.6e-73 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1454 - 1612 8.7e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1772 - 2001 1.6e-129 PRP8 domain IV core
JAB PF01398 2112 - 2211 3.7e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2224 - 2344 1.3e-48 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1536, 1593, 2892
AasI GACNNNNNNGTC 1 cut(s) 5529
Acc36I ACCTGC 4 cut(s) 355, 1078, 3954, 6689
AccB1I GGYRCC 3 cut(s) 32, 1924, 2019
AccB7I CCANNNNNTGG 3 cut(s) 1913, 5447, 6651
AccI GTMKAC 2 cut(s) 641, 3794
AccII CGCG 1 cut(s) 7026
AccIII TCCGGA 2 cut(s) 3385, 6856
AcoI YGGCCR 3 cut(s) 13, 3434, 4946
AcuI CTGAAG 8 cut(s) 1406, 1524, 1625, 3119, 4045, 5335, 5513, 5918
AcyI GRCGYC 2 cut(s) 2020, 4240
AdeI CACNNNGTG 2 cut(s) 4049, 4187
AfeI AGCGCT 1 cut(s) 3834
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 6 cut(s) 223, 1773, 3727, 4186, 5109, 6751
AgeI ACCGGT 2 cut(s) 1875, 6863
AhdI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
AjiI CACGTC 1 cut(s) 6752
AjuI GAANNNNNNNTTGG 4 cut(s) 1706, 1738, 3393, 3425
AleI CACNNNNGTG 5 cut(s) 1930, 2471, 5135, 5508, 5510
AloI GAACNNNNNNTCC 4 cut(s) 3597, 3629, 4504, 4536
Alw21I GWGCWC 5 cut(s) 304, 2478, 4774, 5703, 5863
Alw26I GTCTC 6 cut(s) 2381, 2633, 3125, 3266, 3518, 6097
Alw44I GTGCAC 2 cut(s) 2474, 5859
Ama87I CYCGRG 2 cut(s) 3201, 5076
Aor13HI TCCGGA 2 cut(s) 3385, 6856
Aor51HI AGCGCT 1 cut(s) 3834
ApaLI GTGCAC 2 cut(s) 2474, 5859
ArsI GACNNNNNNTTYG 2 cut(s) 4225, 4257
AsiGI ACCGGT 2 cut(s) 1875, 6863
Asp700I GAANNNNTTC 2 cut(s) 1191, 6374
AspLEI GCGC 6 cut(s) 25, 177, 2022, 2187, 2682, 3835
AsuC2I CCSGG 2 cut(s) 133, 371
AvaI CYCGRG 2 cut(s) 3201, 5076
AxyI CCTNAGG 4 cut(s) 3787, 5264, 6447, 6606
BaeGI GKGCMC 4 cut(s) 1927, 2478, 4032, 5863
BaeI ACNNNNGTAYC 6 cut(s) 275, 308, 857, 890, 5373, 5406
BalI TGGCCA 2 cut(s) 3436, 4948
BamHI GGATCC 2 cut(s) 1057, 5644
BanI GGYRCC 3 cut(s) 32, 1924, 2019
BanII GRGCYC 3 cut(s) 4539, 4614, 5703
BarI GAAGNNNNNNTAC 6 cut(s) 371, 403, 2311, 2343, 3603, 3635
BauI CACGAG 5 cut(s) 2657, 2935, 3341, 3351, 6953
BbsI GAAGAC 3 cut(s) 1505, 1622, 3125
Bbv12I GWGCWC 5 cut(s) 304, 2478, 4774, 5703, 5863
BbvCI CCTCAGC 1 cut(s) 6594
BceAI ACGGC 6 cut(s) 28, 308, 706, 1659, 3293, 6934
BciVI GTATCC 3 cut(s) 3407, 3612, 6779
BclI TGATCA 3 cut(s) 2800, 5956, 6529
BcnI CCSGG 2 cut(s) 133, 371
BcoDI GTCTC 6 cut(s) 2381, 2633, 3125, 3266, 3518, 6097
BfaI CTAG 9 cut(s) 593, 749, 2360, 3300, 3663, 3960, 4700, 4962, 6906
BfmI CTRYAG 7 cut(s) 1485, 2331, 2859, 3711, 5310, 5559, 6682
BfoI RGCGCY 4 cut(s) 2023, 2188, 2683, 3836
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
BfuI GTATCC 3 cut(s) 3407, 3612, 6779
BglII AGATCT 3 cut(s) 1274, 4604, 5346
BlpI GCTNAGC 3 cut(s) 69, 114, 4974
BmcAI AGTACT 2 cut(s) 5015, 6947
BmeRI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
BmeT110I CYCGRG 2 cut(s) 3201, 5076
BmgBI CACGTC 1 cut(s) 6752
BmrI ACTGGG 2 cut(s) 408, 1896
BmuI ACTGGG 2 cut(s) 408, 1896
BoxI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
BpiI GAAGAC 3 cut(s) 1505, 1622, 3125
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 5 cut(s) 81, 2582, 5687, 6678, 7016
Bpu10I CCTNAGC 2 cut(s) 4224, 6594
Bpu1102I GCTNAGC 3 cut(s) 69, 114, 4974
BpuEI CTTGAG 4 cut(s) 359, 2795, 4511, 7001
BpuMI CCSGG 2 cut(s) 133, 371
BsaAI YACGTR 2 cut(s) 3128, 3728
BsaBI GATNNNNATC 3 cut(s) 1062, 2793, 3474
BsaHI GRCGYC 2 cut(s) 2020, 4240
BsaI GGTCTC 2 cut(s) 2633, 3125
BsaWI WCCGGW 5 cut(s) 1875, 3385, 6436, 6856, 6863
Bse118I RCCGGY 5 cut(s) 35, 61, 1875, 3218, 6863
Bse21I CCTNAGG 4 cut(s) 3787, 5264, 6447, 6606
Bse3DI GCAATG 6 cut(s) 1169, 5229, 6384, 6468, 6480, 6656
Bse8I GATNNNNATC 3 cut(s) 1062, 2793, 3474
BseAI TCCGGA 2 cut(s) 3385, 6856
BseJI GATNNNNATC 3 cut(s) 1062, 2793, 3474
BseMI GCAATG 6 cut(s) 1169, 5229, 6384, 6468, 6480, 6656
BseRI GAGGAG 6 cut(s) 1163, 3359, 3499, 4580, 6594, 7013
BseSI GKGCMC 4 cut(s) 1927, 2478, 4032, 5863
BseYI CCCAGC 3 cut(s) 1106, 1987, 6576
BsgI GTGCAG 3 cut(s) 4715, 4883, 5559
Bsh1236I CGCG 1 cut(s) 7026
BshNI GGYRCC 3 cut(s) 32, 1924, 2019
BshTI ACCGGT 2 cut(s) 1875, 6863
BsiHKAI GWGCWC 5 cut(s) 304, 2478, 4774, 5703, 5863
BsiHKCI CYCGRG 2 cut(s) 3201, 5076
BslFI GGGAC 8 cut(s) 257, 299, 2945, 3272, 4133, 6201, 6505, 6762
BsmAI GTCTC 6 cut(s) 2381, 2633, 3125, 3266, 3518, 6097
BsmBI CGTCTC 2 cut(s) 2381, 3518
BsmFI GGGAC 8 cut(s) 257, 299, 2945, 3272, 4133, 6201, 6505, 6762
BsmI GAATGC 7 cut(s) 525, 843, 971, 2702, 3650, 5170, 5831
Bso31I GGTCTC 2 cut(s) 2633, 3125
BsoBI CYCGRG 2 cut(s) 3201, 5076
Bsp1286I GDGCHC 9 cut(s) 304, 1927, 2478, 4032, 4539, 4614, 4774, 5703, 5863
Bsp13I TCCGGA 2 cut(s) 3385, 6856
Bsp1407I TGTACA 2 cut(s) 1041, 5026
Bsp1720I GCTNAGC 3 cut(s) 69, 114, 4974
Bsp19I CCATGG 5 cut(s) 247, 356, 1032, 4157, 6869
Bsp68I TCGCGA 1 cut(s) 7026
BspEI TCCGGA 2 cut(s) 3385, 6856
BspFNI CGCG 1 cut(s) 7026
BspHI TCATGA 6 cut(s) 277, 3459, 4111, 4792, 5235, 6532
BspMAI CTGCAG 4 cut(s) 2863, 3715, 5563, 6686
BspMI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
BspQI GCTCTTC 2 cut(s) 4544, 6528
BspT107I GGYRCC 3 cut(s) 32, 1924, 2019
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 2 cut(s) 2633, 3125
BsrDI GCAATG 6 cut(s) 1169, 5229, 6384, 6468, 6480, 6656
BsrFI RCCGGY 5 cut(s) 35, 61, 1875, 3218, 6863
BsrGI TGTACA 2 cut(s) 1041, 5026
BssAI RCCGGY 5 cut(s) 35, 61, 1875, 3218, 6863
BssNAI GTATAC 1 cut(s) 642
BssNI GRCGYC 2 cut(s) 2020, 4240
BssSI CACGAG 5 cut(s) 2657, 2935, 3341, 3351, 6953
Bst1107I GTATAC 1 cut(s) 642
Bst2BI CACGAG 5 cut(s) 2657, 2935, 3341, 3351, 6953
BstACI GRCGYC 2 cut(s) 2020, 4240
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 4202
BstAUI TGTACA 2 cut(s) 1041, 5026
BstBAI YACGTR 2 cut(s) 3128, 3728
BstC8I GCNNGC 5 cut(s) 63, 2700, 2859, 3434, 5005
BstDSI CCRYGG 5 cut(s) 247, 356, 1032, 4157, 6869
BstEII GGTNACC 2 cut(s) 942, 4650
BstENI CCTNNNNNAGG 1 cut(s) 6998
BstFNI CGCG 1 cut(s) 7026
BstH2I RGCGCY 4 cut(s) 2023, 2188, 2683, 3836
BstHHI GCGC 6 cut(s) 25, 177, 2022, 2187, 2682, 3835
BstMAI GTCTC 6 cut(s) 2381, 2633, 3125, 3266, 3518, 6097
BstNSI RCATGY 7 cut(s) 214, 227, 349, 1543, 3601, 4018, 5113
BstPAI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
BstPI GGTNACC 2 cut(s) 942, 4650
BstSFI CTRYAG 7 cut(s) 1485, 2331, 2859, 3711, 5310, 5559, 6682
BstSLI GKGCMC 4 cut(s) 1927, 2478, 4032, 5863
BstUI CGCG 1 cut(s) 7026
BstV2I GAAGAC 3 cut(s) 1505, 1622, 3125
BstXI CCANNNNNNTGG 5 cut(s) 357, 398, 451, 5339, 6486
BstZ17I GTATAC 1 cut(s) 642
Bsu36I CCTNAGG 4 cut(s) 3787, 5264, 6447, 6606
BsuI GTATCC 3 cut(s) 3407, 3612, 6779
BtgI CCRYGG 5 cut(s) 247, 356, 1032, 4157, 6869
BtrI CACGTC 1 cut(s) 6752
BtsI GCAGTG 3 cut(s) 2954, 3060, 5826
BtuMI TCGCGA 1 cut(s) 7026
BveI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
Cac8I GCNNGC 5 cut(s) 63, 2700, 2859, 3434, 5005
CciI TCATGA 6 cut(s) 277, 3459, 4111, 4792, 5235, 6532
CfoI GCGC 6 cut(s) 25, 177, 2022, 2187, 2682, 3835
Cfr10I RCCGGY 5 cut(s) 35, 61, 1875, 3218, 6863
CseI GACGC 5 cut(s) 2390, 3014, 4229, 5519, 6409
CspAI ACCGGT 2 cut(s) 1875, 6863
CspCI CAANNNNNGTGG 2 cut(s) 2016, 2051
DinI GGCGCC 1 cut(s) 2021
DraIII CACNNNGTG 2 cut(s) 4049, 4187
DrdI GACNNNNNNGTC 1 cut(s) 5529
DriI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
DseDI GACNNNNNNGTC 1 cut(s) 5529
EaeI YGGCCR 3 cut(s) 13, 3434, 4946
Eam1105I GACNNNNNGTC 3 cut(s) 808, 2346, 5110
EciI GGCGGA 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 5701
Eco24I GRGCYC 3 cut(s) 4539, 4614, 5703
Eco31I GGTCTC 2 cut(s) 2633, 3125
Eco32I GATATC 3 cut(s) 1162, 3371, 4749
Eco47III AGCGCT 1 cut(s) 3834
Eco53kI GAGCTC 1 cut(s) 5701
Eco57I CTGAAG 8 cut(s) 1406, 1524, 1625, 3119, 4045, 5335, 5513, 5918
Eco81I CCTNAGG 4 cut(s) 3787, 5264, 6447, 6606
Eco88I CYCGRG 2 cut(s) 3201, 5076
Eco91I GGTNACC 2 cut(s) 942, 4650
EcoICRI GAGCTC 1 cut(s) 5701
EcoNI CCTNNNNNAGG 1 cut(s) 6998
EcoO109I RGGNCCY 1 cut(s) 6492
EcoO65I GGTNACC 2 cut(s) 942, 4650
EcoRI GAATTC 3 cut(s) 3242, 3946, 6313
EcoRV GATATC 3 cut(s) 1162, 3371, 4749
EcoT22I ATGCAT 3 cut(s) 2106, 2794, 6671
EcoT38I GRGCYC 3 cut(s) 4539, 4614, 5703
EgeI GGCGCC 1 cut(s) 2021
EheI GGCGCC 1 cut(s) 2021
Esp3I CGTCTC 2 cut(s) 2381, 3518
FaqI GGGAC 8 cut(s) 257, 299, 2945, 3272, 4133, 6201, 6505, 6762
FauI CCCGC 1 cut(s) 1501
FauNDI CATATG 3 cut(s) 2431, 2927, 4330
FbaI TGATCA 3 cut(s) 2800, 5956, 6529
FblI GTMKAC 2 cut(s) 641, 3794
FriOI GRGCYC 3 cut(s) 4539, 4614, 5703
FspBI CTAG 9 cut(s) 593, 749, 2360, 3300, 3663, 3960, 4700, 4962, 6906
GlaI GCGC 6 cut(s) 24, 176, 2021, 2186, 2681, 3834
GsaI CCCAGC 3 cut(s) 1110, 1991, 6580
GsuI CTGGAG 5 cut(s) 81, 2582, 5687, 6678, 7016
HaeII RGCGCY 4 cut(s) 2023, 2188, 2683, 3836
HgaI GACGC 5 cut(s) 2390, 3014, 4229, 5519, 6409
HhaI GCGC 6 cut(s) 25, 177, 2022, 2187, 2682, 3835
Hin1I GRCGYC 2 cut(s) 2020, 4240
Hin6I GCGC 6 cut(s) 23, 175, 2020, 2185, 2680, 3833
HinP1I GCGC 6 cut(s) 23, 175, 2020, 2185, 2680, 3833
HincII GTYRAC 5 cut(s) 1692, 1797, 3198, 3817, 4590
HindII GTYRAC 5 cut(s) 1692, 1797, 3198, 3817, 4590
HindIII AAGCTT 5 cut(s) 116, 1010, 1697, 2543, 6926
Hpy99I CGWCG 3 cut(s) 194, 507, 1293
HpyCH4IV ACGT 9 cut(s) 582, 1775, 2311, 2830, 3127, 3727, 4186, 4499, 6751
HpySE526I ACGT 9 cut(s) 582, 1775, 2311, 2830, 3127, 3727, 4186, 4499, 6751
Hsp92I GRCGYC 2 cut(s) 2020, 4240
HspAI GCGC 6 cut(s) 23, 175, 2020, 2185, 2680, 3833
KasI GGCGCC 1 cut(s) 2019
KflI GGGWCCC 1 cut(s) 6492
Kpn2I TCCGGA 2 cut(s) 3385, 6856
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 3 cut(s) 2800, 5956, 6529
LguI GCTCTTC 2 cut(s) 4544, 6528
MaeI CTAG 9 cut(s) 593, 749, 2360, 3300, 3663, 3960, 4700, 4962, 6906
MaeII ACGT 9 cut(s) 582, 1775, 2311, 2830, 3127, 3727, 4186, 4499, 6751
MfeI CAATTG 2 cut(s) 605, 1751
MhlI GDGCHC 9 cut(s) 304, 1927, 2478, 4032, 4539, 4614, 4774, 5703, 5863
MlsI TGGCCA 2 cut(s) 3436, 4948
MluNI TGGCCA 2 cut(s) 3436, 4948
Mly113I GGCGCC 1 cut(s) 2020
Mox20I TGGCCA 2 cut(s) 3436, 4948
Mph1103I ATGCAT 3 cut(s) 2106, 2794, 6671
MreI CGCCGGCG 1 cut(s) 61
MroI TCCGGA 2 cut(s) 3385, 6856
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 2 cut(s) 1191, 6374
MscI TGGCCA 2 cut(s) 3436, 4948
Msp20I TGGCCA 2 cut(s) 3436, 4948
MspA1I CMGCKG 6 cut(s) 101, 1229, 4126, 5209, 6151, 6465
MspCI CTTAAG 1 cut(s) 305
MunI CAATTG 2 cut(s) 605, 1751
Mva1269I GAATGC 7 cut(s) 525, 843, 971, 2702, 3650, 5170, 5831
MvnI CGCG 1 cut(s) 7026
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 1 cut(s) 2020
NciI CCSGG 2 cut(s) 133, 371
NcoI CCATGG 5 cut(s) 247, 356, 1032, 4157, 6869
NdeI CATATG 3 cut(s) 2431, 2927, 4330
NgoMIV GCCGGC 1 cut(s) 61
NmeAIII GCCGAG 1 cut(s) 133
NruI TCGCGA 1 cut(s) 7026
NsiI ATGCAT 3 cut(s) 2106, 2794, 6671
NspI RCATGY 7 cut(s) 214, 227, 349, 1543, 3601, 4018, 5113
OliI CACNNNNGTG 5 cut(s) 1930, 2471, 5135, 5508, 5510
PagI TCATGA 6 cut(s) 277, 3459, 4111, 4792, 5235, 6532
PasI CCCWGGG 1 cut(s) 6939
PciI ACATGT 2 cut(s) 223, 5109
PciSI GCTCTTC 2 cut(s) 4544, 6528
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 1288
PctI GAATGC 7 cut(s) 525, 843, 971, 2702, 3650, 5170, 5831
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 2 cut(s) 1191, 6374
PflMI CCANNNNNTGG 3 cut(s) 1913, 5447, 6651
PfoI TCCNGGA 1 cut(s) 369
PinAI ACCGGT 2 cut(s) 1875, 6863
PluTI GGCGCC 1 cut(s) 2023
Ppu21I YACGTR 2 cut(s) 3128, 3728
PpuMI RGGWCCY 1 cut(s) 6492
PscI ACATGT 2 cut(s) 223, 5109
PshAI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
PsiI TTATAA 3 cut(s) 1536, 1593, 2892
Psp124BI GAGCTC 1 cut(s) 5703
Psp5II RGGWCCY 1 cut(s) 6492
PspEI GGTNACC 2 cut(s) 942, 4650
PspFI CCCAGC 3 cut(s) 1106, 1987, 6576
PspPPI RGGWCCY 1 cut(s) 6492
PstI CTGCAG 4 cut(s) 2863, 3715, 5563, 6686
PvuII CAGCTG 4 cut(s) 101, 1229, 4126, 6151
RruI TCGCGA 1 cut(s) 7026
SacI GAGCTC 1 cut(s) 5703
SapI GCTCTTC 2 cut(s) 4544, 6528
ScaI AGTACT 2 cut(s) 5015, 6947
SduI GDGCHC 9 cut(s) 304, 1927, 2478, 4032, 4539, 4614, 4774, 5703, 5863
SfcI CTRYAG 7 cut(s) 1485, 2331, 2859, 3711, 5310, 5559, 6682
SfoI GGCGCC 1 cut(s) 2021
SgrAI CRCCGGYG 2 cut(s) 35, 61
SmlI CTYRAG 5 cut(s) 305, 338, 2774, 4490, 6980
SmoI CTYRAG 5 cut(s) 305, 338, 2774, 4490, 6980
SspDI GGCGCC 1 cut(s) 2019
SspI AATATT 5 cut(s) 2807, 3046, 3161, 4716, 5791
SspMI CTAG 9 cut(s) 593, 749, 2360, 3300, 3663, 3960, 4700, 4962, 6906
SstI GAGCTC 1 cut(s) 5703
TaiI ACGT 9 cut(s) 585, 1778, 2314, 2833, 3130, 3730, 4189, 4502, 6754
TaqII GACCGA 1 cut(s) 930
TatI WGTACW 5 cut(s) 960, 1041, 5013, 5026, 6945
TauI GCSGC 2 cut(s) 28, 68
Van91I CCANNNNNTGG 3 cut(s) 1913, 5447, 6651
Vha464I CTTAAG 1 cut(s) 305
VneI GTGCAC 2 cut(s) 2474, 5859
XagI CCTNNNNNAGG 1 cut(s) 6998
XbaI TCTAGA 1 cut(s) 3299
XceI RCATGY 7 cut(s) 214, 227, 349, 1543, 3601, 4018, 5113
XcmI CCANNNNNNNNNTGG 2 cut(s) 4415, 5934
XmiI GTMKAC 2 cut(s) 641, 3794
XmnI GAANNNNTTC 2 cut(s) 1191, 6374
XspI CTAG 9 cut(s) 593, 749, 2360, 3300, 3663, 3960, 4700, 4962, 6906
ZrmI AGTACT 2 cut(s) 5015, 6947
Zsp2I ATGCAT 3 cut(s) 2106, 2794, 6671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.