Rh2DG573000

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000798
Physical Location & Seq
Forward (+)
80307334 .. 80312838
5505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG573000.1

Sequence Viewer

Length: 5505 bp
ATGCTAAATCTGCTGATACATAGGAAAGGTCTGAACTATCTTCACCTTGATTATAATTTTAATCTCAAGCCTGTGAAAACCCTGACAACAAAAGAGCGTAAGAAATCTCGGTTTGGTAATTCTTTTCATCTGTTGCGTGAAATCTTGCGGTTAACAAAGCTTGTAGTTGATGCCCAGGTCCAGTTCCGTTTGGGTAATATTGATGCCTTTCAATTGGCTGATGGTCTGCAGTACATATTTTCTCATGTTGGTCAGTTAACTGGAATGTACCGTTACAAGTATAGGTTGATGCGGCAGATTAGAATGAGCAAAGATTTGAAGCACTTGATTTACTACCGTTTCAATACTGGGCCGGTCGGGAAAGGGCCGGGATGTGGATTCTGGGCACCAATGTGGAGGGTGTGGTTGTCCTTCCTTCGTGGCATGGTCCCTCTTCTGGAACAGTGGTTGGGAAATTTACTTGCTAGACAATTTGAAGGGCGCCATTCTGTGGCAAAGACAGTAACTAAGCAGCGTGTTGAAAGCAATTTCAACTTGGAGTTTCGAGCAGATGTCTTGAAAGATATTCTGGATGCCATGCCAGTGGGCATGAAGCAAAATAAGGCGAAAACAATTATGCAGCATCTCAGTGAGGCATGGCGTTGTTGGAAAGCAAATATTCCTTGGAAGGTTCCTGGTATGCCTGCTCCGGTTGAGAATATAATTCTTCGTTATGTCAAGTCAAAGGCAGATTGGTGGACACATGGTGCTCATTATAACCGTGAACGTATTAGAAGAGGTGCTACAGTGGATAAGATAGTTTGTCGAAAGAATCTTGGCAGATTAACTCGCCTTTGGCTAAAGGGAGAGCAGGAGCGACAGCACAATTATCTCAAAGATGGTCCATATATTTCACCAGAAGAAGCAGTAGCAATTTACTCAACAACTGTGCATTGGTTGGAGTCAAGAAATTTCCAACATATTCCATTTCCCCCTTTGTCGTATAAGCATGATACCAAGCTACTTATCCTTGCTCTGGAGAGGTTGAAGGAATCTTATACTGCAGCAGTAAAATTGAACCAGCATCAAAGAGAGGAGTTGGGCCTCATTGAACAAGCCTATGATAACCCCCATGAGGCATTGATACGGATAAAGCGTCACTTGATCTCACAGCGTGCCTTCAAAGACGTGAGCATAGAGTTCATGGATCTGTACAGCCATCATATTCCAGTTTTTGAGATTGACCCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGCTGACAAACGTCAGCTCTTCCCGAACTGGATCAAGCCTGCAGACTCAGAGCCACCTCCACTTTTGGTCTATAAATGGTGTCAGGGGATAAACAATTTAGAGAGTATATGGGACACAAGTGAGGGGGAGTGTGTTGTGATGCTTCAGACAAAACTTGAGAGATTATCTGAGAAGATTGACTTGACATTGCTAAATAGGCTTCTACGTCTTGTTCTTGACCATAACATTGCTGATTATATCACTGCAAAAAACAATGTGGTATTGTCTTACAAAGATATGAGTCATACAAATTCATATGGTCTTATACGGGGTCTCCTGCCTGCATCATTTGTTGTACAGTATTATGGGCTTGTTTTGGATCTAATGCTTCTTGGATTGACTCGGGCCAATGAAATTGCAGGTCCACAGCAGAAGCCAAATGAGTTCATGACTTATGGGGCCACAAAGGTTGAGACTCGCCATCCTATCAGGCTGTACTCTCGATACGTAGACAGGGTGCATATCTTGTTCCGCTTCACTCAGGAGGAGGCTCGGGACCTCATACAGCGATATCTTACTGAGCATCCAGATCCCAATAATGAAAATATGGTTGGATATAATAATAAGAAGTGCTGGGCAAGAAATGCGAGGATGAGGCTCCTGAAACATGATGTCAATCTTGGAAGGAGTGTCTTTTGGAATGTCAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAATGGGAGGACAGTTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTTTTCAGCATGTGTGGATTTGAAGTAAGAATACTTCCCAAGATAAGAATGAGTACGAAGGAACACCAAAGAGATGGAGTTTGGAACTTGCAAAATGAACAGACAAAGGAAAGGACTGCTGTTGCTATTTTACGTGTTGAGGATGAATACATGAAGGCGTTTGAGAATCGTGTAAGGCAGATCCTTATGTCCTCAGGGTCTACAACATTTACAAAAGTTATCAACAAATGGAATACTGCCCTTATTGGTCTTATGACTTACTTCCGTGAAGCAGTTGTACATACAGCGGAGCTGTTAGATTTACTGGTCAAATGTGAAAATAAAATTCAGACCCGCATTAAGATTGGATTGAATTCAAAGATGCCAAGCAGGTTCCCACCTGTCATTTTTTACACGCCAAAAGAAATTGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAGAGTGACCTCAGATACTCTTGGCAGACAGAAATTGGTGTGACACACTTTAGGAGTGGAATGAGTCATGAAGAAGAGCAGCTGATTCCAAATCTTTACCGTTACATACAACCATGGGAGAGTGAGTTTATAGATTCACAGCGTGTTTGGGCAGAATATGCACTAAAAAGACAGGAAGCTATGGAACAGAATCGGCGTCTTACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACATTGTTCCAGAAGGATCGACACACTTTAGCATATGACAAAGGGTGGAGAGTTCGCACAGATTTTAAGCAGTACCAGATCTTGAAGCAAAATCCTTTCTGGTGGACACACCAAAGGCATGATGGAAAATTGTGGAATTTGAATAATTATAGAACTGATGTGATCCAAGCTCTTGGAGGAGTTGAGGGAATACTTGAGCACACCTTATTCAAAGGAACATACTTCCCTACTTGGGAGGGTCTCTTTTGGGAGAAGGCATCTGGATTTGAGGAATCGATGAAGTATAAAAAGTTGACTAATGCCCAAAGATCTGGCCTCAACCAAATCCCCAATCGTAGGTTTACACTTTGGTGGTCGCCTACCATAAATCGAGCCAATGTATATGTCGGTTTTCAAGTACAGCTGGATCTGACAGGAATCTTTATGCATGGGAAGATCCCAACCTTGAAGATATCGTTGATTCAGATATTCCGTGCACACTTGTGGCAGAAGATTCATGAAAGTGTTGTGATGGATCTTTGTCAGGTTTTGGATCAGGAGTTGGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACCATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCAGACATTCTCCTCTTTTCTGCTCATAAATGGCAAATGTCGAAACCTAGTCTTGTGGCTGAGCCGAAGGATGAGTATGATAAAAAAACAAGCAACAAGTACTGGATAGACGTGCAGCTCCGTTGGGGGGATTATGATTCTCATGATATTGAACGTTACACCCGGGCAAAGTTTATGGACTATACAACTGATAACATGTCTATCTATCCTTCTCCGACCGGAGTGATGATTGGTATTGATTTAGCCTATAATGTGCATTCTGCATTTGGTAACTGGTTTCCCGGATCAAAACCACTGATTCAACAGGCAATGAACAAGATCATGAAGTCAAATCCGGCTTTATATGTGTTGAGGGAGCGCATAAGGAAAGGATTGCAGTTGTATTCCTCGGAGCCTACAGAGCCATATTTGTCTTCCCAAAACTATGGGGAGCTCTTCAGCAATCAAATTAAATGGTTTGTTGACGATACAAATGTGTATCGTGTTACAATGCACAAGACTTTTGACGGAAATCTTGTTACAAAACCCATCAATGGTGCTATCTTCATGTTCAATCCAGGGACTGGGCAGCTGTTCTTGAAGGTTATCCACACAAGTGTGTGGGCAGGACAGAAGCGACTTGGGCAGTTGGCTAAATGGAAAACTGCAGAAGAAGTTGCTGCTCTTGTGCGTTCTCTACCTGTTGAAGAACAGCCAAAGCAAATTATTGTGACTCGTAAGGGAATGCTCGACCCATTGGAGGTTCATTTGCTGGATTTTCCCAACATAGTCATAAAAGGAAGTGAGCTACAGCTTCCTTTTCAGGTTTGCTTGAAAATTGAGAAGTTGAATGATCTGGTACTAAAGTCTACTGAGCCACAGATGGTTCTTTTTAATGTTTACGATGATTGGTTGAAGAGTGTTTCATCGTACACTGCATTCTCTCGGCTCATTCTGATTCTTCGGGCACTGCATGTGAACAGAGACAAGGCAAGTATGTTGTTGAAGCCTGACCGAACAGTCATTAGTGAGCCACACCACATTTGGCCTTCTCTTTCTGATGAGCAATGGATGAAGGTTGAGCGTGGTCTAACAGATCTCATACTGTCAGACTATGCGAAAAAGAATAATGTGAGTACTTCAGCCCTCACACAGTCTGAAATTCGTGATATAATACTTGGAGCAGAGATTACTCCACCTTCTCAACAGAGGCAAGAGATAGCAGAGATTGAGAAACAGGCTAAAGGAGCTAGTCAGCTGACAGCAGTCACCACAAGGACCACAAACGTGCATGGAGATGAGCTTATTTCCACCACTACGAGTCAATATGAGCAAGCTGCATTCAGGTCCAAAACTGATTGGCATGTGAGAGCAATTTCAGCTACAAATCTACATCTTCGTGTCAACCATATTTATGTGAGTTCAGAAGATACAAAGGAAACATGCTGCACTTACATCATGCCAATGAACATTTTGAAAAAGTTTATCTGTGTTGCTGATCTGTGGACACAAGTTGCTGGTTACTTGTATGGTATAAGCCCACCTGACAACCCTCAGGTTAAGGAAATTCGCTGCATTGTGATGCCACCACAGCGGGGCACCCACCAGCAAGTTCATCTTCCATCAGCTCTTCCTGAGCATGATTTCCTCAATTATTTGGAACCTCTGGGATGGATGCACACCCAGCCAAATGAGCTACCTCAGCTTTCTCCTCAGGATCTCACCACTCATGCTAACATGTTGGAGAATGGCAAGCAATGGGATAGGGAGAAATGCATTATTATGACCTGCGCTTTTACTCCAGGTTCCTGCTCGTTAACTGCATATAAACTTACTCCATCTGGTTATGAGTGGGGATGTTGTTCTAATAAGAACACCGGAAGAAACAATAATCCACTAGATGGATACCTTCCAATTCATTATGAGAAGGTCCAGATGCTTCTCAGTGATCGCTTCCTTGGTTTCTACATGGTTCCTGATAATTGTCCATGGAACTTCAATTTCATGGGAGTGAGGCATGCATGTGACATGAAGTATGGCATCAAGCTTGGACCACCCAAGGAGTACTATCACGAGGAACACCGACCAACCCATTTCATGGAGTTCAGCGAACTAGATAAGGATGACCCGATGGAGTTAGATCGAGATTATGCCTACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

1834

Amino Acids

213.95

Weight (kDa)

8.96

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROCN PF08083 1 - 297 2.2e-173 PROCN (NUC071) domain
RRM_4 PF10598 482 - 571 5.8e-44 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 732 - 867 6e-74 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 937 - 1095 6.6e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1255 - 1484 5.7e-125 PRP8 domain IV core
JAB PF01398 1605 - 1693 5.5e-10 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 1707 - 1829 1.9e-42 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 756
AasI GACNNNNNNGTC 1 cut(s) 4361
Acc36I ACCTGC 3 cut(s) 1664, 2403, 5138
AccB1I GGYRCC 3 cut(s) 385, 480, 4940
AccB7I CCANNNNNTGG 4 cut(s) 490, 3926, 5243, 5440
AccI GTMKAC 3 cut(s) 1764, 2243, 4211
AciI CCGC 6 cut(s) 148, 292, 1786, 2330, 2377, 4936
AclI AACGTT 1 cut(s) 3517
AcsI RAATTY 9 cut(s) 454, 949, 1564, 2038, 2367, 2395, 2881, 4503, 4908
AcuI CTGAAG 3 cut(s) 1403, 3784, 4467
AcyI GRCGYC 2 cut(s) 481, 2689
AdeI CACNNNGTG 4 cut(s) 746, 1154, 2498, 2636
AflIII ACRYGT 3 cut(s) 2176, 3558, 5079
AjiI CACGTC 2 cut(s) 1168, 3475
AjnI CCWGG 5 cut(s) 174, 673, 3332, 3919, 5143
AjuI GAANNNNNNNTTGG 2 cut(s) 1848, 1880
AleI CACNNNNGTG 6 cut(s) 391, 3094, 3226, 3957, 3959, 4628
Alw21I GWGCWC 4 cut(s) 751, 2946, 3223, 3798
Alw26I GTCTC 6 cut(s) 1592, 1721, 1973, 2990, 3299, 4320
Alw44I GTGCAC 1 cut(s) 3219
AlwNI CAGNNNCTG 1 cut(s) 3926
Ama87I CYCGRG 3 cut(s) 1656, 1806, 3525
AoxI GGCC 7 cut(s) 350, 365, 1081, 1659, 1713, 3058, 4388
ApaLI GTGCAC 1 cut(s) 3219
ApoI RAATTY 9 cut(s) 454, 949, 1564, 2038, 2367, 2395, 2881, 4503, 4908
AspLEI GCGC 3 cut(s) 483, 3723, 5135
AsuC2I CCSGG 4 cut(s) 369, 3526, 3527, 3645
AsuHPI GGTGA 4 cut(s) 35, 885, 4603, 5056
AvaI CYCGRG 3 cut(s) 1656, 1806, 3525
AvaII GGWCC 9 cut(s) 178, 427, 881, 1676, 1810, 4620, 4689, 5272, 5393
AxyI CCTNAGG 3 cut(s) 2236, 4896, 5055
BaeGI GKGCMC 4 cut(s) 388, 3223, 4312, 4943
BaeI ACNNNNGTAYC 4 cut(s) 3822, 3855, 4193, 4226
BanI GGYRCC 3 cut(s) 385, 480, 4940
BanII GRGCYC 1 cut(s) 3798
BarI GAAGNNNNNNTAC 8 cut(s) 766, 798, 2058, 2061, 2090, 2093, 2304, 2336
BauI CACGAG 1 cut(s) 5414
BbsI GAAGAC 1 cut(s) 3768
Bbv12I GWGCWC 4 cut(s) 751, 2946, 3223, 3798
BbvCI CCTCAGC 1 cut(s) 5043
BciT130I CCWGG 5 cut(s) 176, 675, 3334, 3921, 5145
BciVI GTATCC 1 cut(s) 5240
BclI TGATCA 1 cut(s) 1255
BcnI CCSGG 4 cut(s) 369, 3526, 3527, 3645
BcoDI GTCTC 6 cut(s) 1592, 1721, 1973, 2990, 3299, 4320
BfaI CTAG 5 cut(s) 465, 3411, 4593, 5240, 5456
BfmI CTRYAG 7 cut(s) 227, 783, 1041, 1314, 3759, 4008, 4151
BfoI RGCGCY 1 cut(s) 484
BfuAI ACCTGC 3 cut(s) 1664, 2403, 5138
BfuI GTATCC 1 cut(s) 5240
BglII AGATCT 3 cut(s) 2823, 3053, 4438
BlpI GCTNAGC 1 cut(s) 3423
BmcAI AGTACT 3 cut(s) 3464, 4480, 5408
Bme1390I CCNGG 9 cut(s) 176, 369, 675, 3334, 3526, 3527, 3645, 3921, 5145
Bme18I GGWCC 9 cut(s) 178, 427, 881, 1676, 1810, 4620, 4689, 5272, 5393
BmeT110I CYCGRG 3 cut(s) 1656, 1806, 3525
BmgBI CACGTC 2 cut(s) 1168, 3475
BmrFI CCNGG 9 cut(s) 176, 369, 675, 3334, 3526, 3527, 3645, 3921, 5145
BmrI ACTGGG 2 cut(s) 357, 3936
BmuI ACTGGG 2 cut(s) 357, 3936
BoxI GACNNNNGTC 2 cut(s) 1284, 4607
BpiI GAAGAC 1 cut(s) 3768
BpmI CTGGAG 2 cut(s) 1037, 5127
Bpu10I CCTNAGC 2 cut(s) 4977, 5043
Bpu1102I GCTNAGC 1 cut(s) 3423
BpuEI CTTGAG 4 cut(s) 50, 1250, 1451, 2960
BpuMI CCSGG 4 cut(s) 369, 3526, 3527, 3645
Bsa29I ATCGAT 1 cut(s) 3020
BsaAI YACGTR 2 cut(s) 1762, 2177
BsaBI GATNNNNATC 3 cut(s) 1248, 1260, 1929
BsaHI GRCGYC 2 cut(s) 481, 2689
BsaI GGTCTC 2 cut(s) 1592, 2990
BsaWI WCCGGW 3 cut(s) 688, 3581, 5219
BsaXI ACNNNNNCTCC 4 cut(s) 1572, 1602, 2603, 2633
Bse118I RCCGGY 1 cut(s) 352
Bse21I CCTNAGG 3 cut(s) 2236, 4896, 5055
Bse3DI GCAATG 5 cut(s) 1460, 1500, 3678, 4415, 5105
Bse8I GATNNNNATC 3 cut(s) 1248, 1260, 1929
BseBI CCWGG 5 cut(s) 176, 675, 3334, 3921, 5145
BseCI ATCGAT 1 cut(s) 3020
BseJI GATNNNNATC 3 cut(s) 1248, 1260, 1929
BseMI GCAATG 5 cut(s) 1460, 1500, 3678, 4415, 5105
BseRI GAGGAG 5 cut(s) 1088, 1814, 2937, 3365, 5043
BseSI GKGCMC 4 cut(s) 388, 3223, 4312, 4943
BseYI CCCAGC 2 cut(s) 1887, 5025
BsgI GTGCAG 4 cut(s) 3332, 3384, 3497, 4774
Bsh1285I CGRYCG 2 cut(s) 357, 3582
BshFI GGCC 7 cut(s) 352, 367, 1083, 1661, 1715, 3060, 4390
BshNI GGYRCC 3 cut(s) 385, 480, 4940
BshVI ATCGAT 1 cut(s) 3020
BsiEI CGRYCG 2 cut(s) 357, 3582
BsiHKAI GWGCWC 4 cut(s) 751, 2946, 3223, 3798
BsiHKCI CYCGRG 3 cut(s) 1656, 1806, 3525
BsiSI CCGG 8 cut(s) 353, 368, 689, 3526, 3582, 3645, 3698, 5220
BslFI GGGAC 4 cut(s) 413, 1400, 1823, 3937
BsmAI GTCTC 6 cut(s) 1592, 1721, 1973, 2990, 3299, 4320
BsmBI CGTCTC 1 cut(s) 1973
BsmFI GGGAC 4 cut(s) 413, 1400, 1823, 3937
BsmI GAATGC 4 cut(s) 3619, 4092, 4280, 4682
BsnI GGCC 7 cut(s) 352, 367, 1083, 1661, 1715, 3060, 4390
Bso31I GGTCTC 2 cut(s) 1592, 2990
BsoBI CYCGRG 3 cut(s) 1656, 1806, 3525
Bsp1286I GDGCHC 7 cut(s) 388, 751, 2946, 3223, 3798, 4312, 4943
Bsp1407I TGTACA 3 cut(s) 1191, 1609, 2320
Bsp1720I GCTNAGC 1 cut(s) 3423
Bsp19I CCATGG 2 cut(s) 2606, 5330
BspACI CCGC 6 cut(s) 148, 292, 1786, 2330, 2377, 4936
BspANI GGCC 7 cut(s) 352, 367, 1083, 1661, 1715, 3060, 4390
BspDI ATCGAT 1 cut(s) 3020
BspHI TCATGA 5 cut(s) 1701, 2560, 3241, 3505, 3684
BspMAI CTGCAG 4 cut(s) 231, 1045, 1318, 4012
BspMI ACCTGC 3 cut(s) 1664, 2403, 5138
BspQI GCTCTTC 4 cut(s) 1298, 2562, 3803, 4977
BspT107I GGYRCC 3 cut(s) 385, 480, 4940
BspTNI GGTCTC 2 cut(s) 1592, 2990
BsrDI GCAATG 5 cut(s) 1460, 1500, 3678, 4415, 5105
BsrFI RCCGGY 1 cut(s) 352
BsrGI TGTACA 3 cut(s) 1191, 1609, 2320
BssAI RCCGGY 1 cut(s) 352
BssNI GRCGYC 2 cut(s) 481, 2689
BssSI CACGAG 1 cut(s) 5414
BssT1I CCWWGG 5 cut(s) 662, 2606, 5299, 5330, 5400
Bst2BI CACGAG 1 cut(s) 5414
Bst2UI CCWGG 5 cut(s) 176, 675, 3334, 3921, 5145
Bst6I CTCTTC 8 cut(s) 438, 769, 1298, 2562, 3332, 3803, 4253, 4977
BstACI GRCGYC 2 cut(s) 481, 2689
BstAPI GCANNNNNTGC 2 cut(s) 1898, 2651
BstAUI TGTACA 3 cut(s) 1191, 1609, 2320
BstBAI YACGTR 2 cut(s) 1762, 2177
BstC8I GCNNGC 7 cut(s) 684, 1155, 1314, 1596, 4677, 5096, 5361
BstDSI CCRYGG 2 cut(s) 2606, 5330
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 3 cut(s) 483, 3723, 5135
BstMAI GTCTC 6 cut(s) 1592, 1721, 1973, 2990, 3299, 4320
BstMCI CGRYCG 2 cut(s) 357, 3582
BstNI CCWGG 5 cut(s) 176, 675, 3334, 3921, 5145
BstNSI RCATGY 9 cut(s) 2056, 2467, 3562, 4319, 4709, 4788, 5083, 5363, 5367
BstPAI GACNNNNGTC 2 cut(s) 1284, 4607
BstSCI CCNGG 9 cut(s) 174, 367, 673, 3332, 3524, 3525, 3643, 3919, 5143
BstSFI CTRYAG 7 cut(s) 227, 783, 1041, 1314, 3759, 4008, 4151
BstSLI GKGCMC 4 cut(s) 388, 3223, 4312, 4943
BstSNI TACGTA 1 cut(s) 1762
BstV2I GAAGAC 1 cut(s) 3768
BstXI CCANNNNNNTGG 5 cut(s) 583, 2117, 2918, 3056, 3788
Bsu15I ATCGAT 1 cut(s) 3020
Bsu36I CCTNAGG 3 cut(s) 2236, 4896, 5055
BsuI GTATCC 1 cut(s) 5240
BsuRI GGCC 7 cut(s) 352, 367, 1083, 1661, 1715, 3060, 4390
BsuTUI ATCGAT 1 cut(s) 3020
BtgI CCRYGG 2 cut(s) 2606, 5330
BtrI CACGTC 2 cut(s) 1168, 3475
BtsI GCAGTG 3 cut(s) 1515, 4275, 4310
BtsIMutI CAGTG 9 cut(s) 449, 588, 634, 792, 1515, 3656, 4275, 4310, 5293
BveI ACCTGC 3 cut(s) 1664, 2403, 5138
Cac8I GCNNGC 7 cut(s) 684, 1155, 1314, 1596, 4677, 5096, 5361
CaiI CAGNNNCTG 1 cut(s) 3926
CciI TCATGA 5 cut(s) 1701, 2560, 3241, 3505, 3684
CfoI GCGC 3 cut(s) 483, 3723, 5135
Cfr10I RCCGGY 1 cut(s) 352
Cfr9I CCCGGG 1 cut(s) 3525
ClaI ATCGAT 1 cut(s) 3020
CseI GACGC 2 cut(s) 1124, 2678
CspCI CAANNNNNGTGG 2 cut(s) 1705, 1740
DinI GGCGCC 1 cut(s) 482
DraIII CACNNNGTG 4 cut(s) 746, 1154, 2498, 2636
DrdI GACNNNNNNGTC 1 cut(s) 4361
DseDI GACNNNNNNGTC 1 cut(s) 4361
Eam1104I CTCTTC 8 cut(s) 438, 769, 1298, 2562, 3332, 3803, 4253, 4977
EarI CTCTTC 8 cut(s) 438, 769, 1298, 2562, 3332, 3803, 4253, 4977
Ecl136II GAGCTC 1 cut(s) 3796
Eco105I TACGTA 1 cut(s) 1762
Eco130I CCWWGG 5 cut(s) 662, 2606, 5299, 5330, 5400
Eco24I GRGCYC 1 cut(s) 3798
Eco31I GGTCTC 2 cut(s) 1592, 2990
Eco32I GATATC 2 cut(s) 1826, 3198
Eco47I GGWCC 9 cut(s) 178, 427, 881, 1676, 1810, 4620, 4689, 5272, 5393
Eco53kI GAGCTC 1 cut(s) 3796
Eco57I CTGAAG 3 cut(s) 1403, 3784, 4467
Eco81I CCTNAGG 3 cut(s) 2236, 4896, 5055
Eco88I CYCGRG 3 cut(s) 1656, 1806, 3525
EcoICRI GAGCTC 1 cut(s) 3796
EcoO109I RGGNCCY 1 cut(s) 1810
EcoRI GAATTC 1 cut(s) 2395
EcoRII CCWGG 5 cut(s) 174, 673, 3332, 3919, 5143
EcoRV GATATC 2 cut(s) 1826, 3198
EcoT14I CCWWGG 5 cut(s) 662, 2606, 5299, 5330, 5400
EcoT22I ATGCAT 4 cut(s) 1249, 3174, 5120, 5365
EcoT38I GRGCYC 1 cut(s) 3798
EgeI GGCGCC 1 cut(s) 482
EheI GGCGCC 1 cut(s) 482
ErhI CCWWGG 5 cut(s) 662, 2606, 5299, 5330, 5400
Esp3I CGTCTC 1 cut(s) 1973
FalI AAGNNNNNCTT 6 cut(s) 1124, 1156, 1439, 1471, 4944, 4976
FaqI GGGAC 4 cut(s) 413, 1400, 1823, 3937
FauI CCCGC 2 cut(s) 2384, 4929
FauNDI CATATG 2 cut(s) 1570, 2779
FbaI TGATCA 1 cut(s) 1255
FblI GTMKAC 3 cut(s) 1764, 2243, 4211
FriOI GRGCYC 1 cut(s) 3798
FspBI CTAG 5 cut(s) 465, 3411, 4593, 5240, 5456
GlaI GCGC 3 cut(s) 482, 3722, 5134
GsaI CCCAGC 2 cut(s) 1891, 5029
GsuI CTGGAG 2 cut(s) 1037, 5127
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 7 cut(s) 352, 367, 1083, 1661, 1715, 3060, 4390
HapII CCGG 8 cut(s) 353, 368, 689, 3526, 3582, 3645, 3698, 5220
HgaI GACGC 2 cut(s) 1124, 2678
HhaI GCGC 3 cut(s) 483, 3723, 5135
Hin1I GRCGYC 2 cut(s) 481, 2689
Hin6I GCGC 3 cut(s) 481, 3721, 5133
HinP1I GCGC 3 cut(s) 481, 3721, 5133
HincII GTYRAC 6 cut(s) 153, 258, 3039, 3826, 4747, 5160
HindII GTYRAC 6 cut(s) 153, 258, 3039, 3826, 4747, 5160
HindIII AAGCTT 2 cut(s) 158, 5387
HpaI GTTAAC 3 cut(s) 153, 258, 5160
HpaII CCGG 8 cut(s) 353, 368, 689, 3526, 3582, 3645, 3698, 5220
HphI GGTGA 4 cut(s) 35, 885, 4603, 5056
HpyCH4IV ACGT 9 cut(s) 766, 1167, 1285, 1480, 1761, 2176, 3474, 3517, 4629
HpySE526I ACGT 9 cut(s) 766, 1167, 1285, 1480, 1761, 2176, 3474, 3517, 4629
Hsp92I GRCGYC 2 cut(s) 481, 2689
HspAI GCGC 3 cut(s) 481, 3721, 5133
KasI GGCGCC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 1255
KspAI GTTAAC 3 cut(s) 153, 258, 5160
LguI GCTCTTC 4 cut(s) 1298, 2562, 3803, 4977
MaeI CTAG 5 cut(s) 465, 3411, 4593, 5240, 5456
MaeII ACGT 9 cut(s) 766, 1167, 1285, 1480, 1761, 2176, 3474, 3517, 4629
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 7 cut(s) 388, 751, 2946, 3223, 3798, 4312, 4943
Mly113I GGCGCC 1 cut(s) 481
MlyI GAGTC 8 cut(s) 950, 1313, 1564, 1648, 1723, 2566, 4069, 4672
MmeI TCCRAC 7 cut(s) 626, 918, 979, 1846, 3267, 3602, 5064
Mph1103I ATGCAT 4 cut(s) 1249, 3174, 5120, 5365
MspA1I CMGCKG 6 cut(s) 2330, 2575, 3148, 3934, 4600, 4936
MspI CCGG 8 cut(s) 353, 368, 689, 3526, 3582, 3645, 3698, 5220
MspR9I CCNGG 9 cut(s) 176, 369, 675, 3334, 3526, 3527, 3645, 3921, 5145
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 4 cut(s) 3619, 4092, 4280, 4682
MvaI CCWGG 5 cut(s) 176, 675, 3334, 3921, 5145
NarI GGCGCC 1 cut(s) 481
NciI CCSGG 4 cut(s) 369, 3526, 3527, 3645
NcoI CCATGG 2 cut(s) 2606, 5330
NdeI CATATG 2 cut(s) 1570, 2779
NmeAIII GCCGAG 1 cut(s) 4267
NmuCI GTSAC 7 cut(s) 1136, 2477, 2498, 2533, 4072, 4609, 5366
NsiI ATGCAT 4 cut(s) 1249, 3174, 5120, 5365
NspI RCATGY 9 cut(s) 2056, 2467, 3562, 4319, 4709, 4788, 5083, 5363, 5367
OliI CACNNNNGTG 6 cut(s) 391, 3094, 3226, 3957, 3959, 4628
PaeI GCATGC 1 cut(s) 5363
PagI TCATGA 5 cut(s) 1701, 2560, 3241, 3505, 3684
PciI ACATGT 2 cut(s) 3558, 5079
PciSI GCTCTTC 4 cut(s) 1298, 2562, 3803, 4977
PcsI WCGNNNNNNNCGW 1 cut(s) 1132
PctI GAATGC 4 cut(s) 3619, 4092, 4280, 4682
PflMI CCANNNNNTGG 4 cut(s) 490, 3926, 5243, 5440
PfoI TCCNGGA 1 cut(s) 3643
PleI GAGTC 8 cut(s) 949, 1313, 1563, 1648, 1723, 2565, 4069, 4671
PluTI GGCGCC 1 cut(s) 484
PpsI GAGTC 8 cut(s) 949, 1313, 1563, 1648, 1723, 2565, 4069, 4671
Ppu21I YACGTR 2 cut(s) 1762, 2177
PpuMI RGGWCCY 1 cut(s) 1810
PscI ACATGT 2 cut(s) 3558, 5079
PshAI GACNNNNGTC 2 cut(s) 1284, 4607
PsiI TTATAA 2 cut(s) 54, 756
Psp124BI GAGCTC 1 cut(s) 3798
Psp1406I AACGTT 1 cut(s) 3517
Psp5II RGGWCCY 1 cut(s) 1810
Psp6I CCWGG 5 cut(s) 174, 673, 3332, 3919, 5143
PspFI CCCAGC 2 cut(s) 1887, 5025
PspGI CCWGG 5 cut(s) 174, 673, 3332, 3919, 5143
PspPPI RGGWCCY 1 cut(s) 1810
PstI CTGCAG 4 cut(s) 231, 1045, 1318, 4012
PstNI CAGNNNCTG 1 cut(s) 3926
PvuII CAGCTG 4 cut(s) 2575, 3148, 3934, 4600
SacI GAGCTC 1 cut(s) 3798
SapI GCTCTTC 4 cut(s) 1298, 2562, 3803, 4977
ScaI AGTACT 3 cut(s) 3464, 4480, 5408
SchI GAGTC 8 cut(s) 950, 1313, 1564, 1648, 1723, 2566, 4069, 4672
ScrFI CCNGG 9 cut(s) 176, 369, 675, 3334, 3526, 3527, 3645, 3921, 5145
SduI GDGCHC 7 cut(s) 388, 751, 2946, 3223, 3798, 4312, 4943
SfcI CTRYAG 7 cut(s) 227, 783, 1041, 1314, 3759, 4008, 4151
SfoI GGCGCC 1 cut(s) 482
SinI GGWCC 9 cut(s) 178, 427, 881, 1676, 1810, 4620, 4689, 5272, 5393
SmaI CCCGGG 1 cut(s) 3527
SmlI CTYRAG 4 cut(s) 65, 1229, 1430, 2939
SmoI CTYRAG 4 cut(s) 65, 1229, 1430, 2939
SnaBI TACGTA 1 cut(s) 1762
SphI GCATGC 1 cut(s) 5363
SsiI CCGC 6 cut(s) 148, 292, 1786, 2330, 2377, 4936
SspDI GGCGCC 1 cut(s) 480
SspI AATATT 2 cut(s) 199, 658
SspMI CTAG 5 cut(s) 465, 3411, 4593, 5240, 5456
SstI GAGCTC 1 cut(s) 3798
StyD4I CCNGG 9 cut(s) 174, 367, 673, 3332, 3524, 3525, 3643, 3919, 5143
StyI CCWWGG 5 cut(s) 662, 2606, 5299, 5330, 5400
TaiI ACGT 9 cut(s) 769, 1170, 1288, 1483, 1764, 2179, 3477, 3520, 4632
TaqII GACCGA 1 cut(s) 4371
TatI WGTACW 9 cut(s) 231, 1191, 1609, 1749, 2320, 3142, 3462, 4478, 5406
TauI GCSGC 1 cut(s) 295
TscAI CASTG 9 cut(s) 449, 588, 634, 792, 1522, 3663, 4282, 4317, 5293
TseFI GTSAC 7 cut(s) 1136, 2477, 2498, 2533, 4072, 4609, 5366
Tsp45I GTSAC 7 cut(s) 1136, 2477, 2498, 2533, 4072, 4609, 5366
TspGWI ACGGA 6 cut(s) 176, 1141, 2297, 3206, 3473, 3885
TspMI CCCGGG 1 cut(s) 3525
TspRI CASTG 9 cut(s) 449, 588, 634, 792, 1522, 3663, 4282, 4317, 5293
Van91I CCANNNNNTGG 4 cut(s) 490, 3926, 5243, 5440
VneI GTGCAC 1 cut(s) 3219
VpaK11BI GGWCC 9 cut(s) 178, 427, 881, 1676, 1810, 4620, 4689, 5272, 5393
XapI RAATTY 9 cut(s) 454, 949, 1564, 2038, 2367, 2395, 2881, 4503, 4908
XceI RCATGY 9 cut(s) 2056, 2467, 3562, 4319, 4709, 4788, 5083, 5363, 5367
XcmI CCANNNNNNNNNTGG 2 cut(s) 2864, 4383
XmaI CCCGGG 1 cut(s) 3525
XmiI GTMKAC 3 cut(s) 1764, 2243, 4211
XspI CTAG 5 cut(s) 465, 3411, 4593, 5240, 5456
ZrmI AGTACT 3 cut(s) 3464, 4480, 5408
Zsp2I ATGCAT 4 cut(s) 1249, 3174, 5120, 5365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.