pycom12419g00050

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012419
OG0000798
Physical Location & Seq
Forward (+)
96603 .. 97843
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12419g00050.1

Sequence Viewer

Length: 318 bp
ATGTCAGGAAGATCGATTAGAGACCATGGGGACATGTCCTCAAAGAAATTTCGTCATGATAAACGAGTGTATCTCGGAGCGCTTAAGTTTGTCCCCCATGCAGTTTACAAGCTCCTCGAGAATATGCCAATGCCATGGGAGCAGGTCCGGGATGTGAAGGTTTTGTACCATATAACTGGGGCAATTACATTTGTAAATGAAATACCATGGGTTGTTGAACCTATCTATTTGGCTCAGTGGGGTACCATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGATCGGCATTTCAAAAGAATGCGGTTTCCACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

106

Amino Acids

12.44

Weight (kDa)

9.99

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 6 - 99 1.3e-52 PRO8NT (NUC069), PrP8 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 133
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AciI CCGC 1 cut(s) 302
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 2 cut(s) 167, 244
AfeI AGCGCT 1 cut(s) 81
AflII CTTAAG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 2 cut(s) 218, 293
AluBI AGCT 1 cut(s) 112
AluI AGCT 1 cut(s) 112
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 116
Aor51HI AGCGCT 1 cut(s) 81
ApoI RAATTY 1 cut(s) 47
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 145
BaeI ACNNNNGTAYC 2 cut(s) 53, 86
BanI GGYRCC 1 cut(s) 242
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpuMI CCSGG 1 cut(s) 149
Bsa29I ATCGAT 2 cut(s) 14, 280
BsaI GGTCTC 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 25, 134, 206
Bse1I ACTGG 1 cut(s) 181
BseCI ATCGAT 2 cut(s) 14, 280
BseDI CCNNGG 3 cut(s) 25, 134, 206
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 242
BshVI ATCGAT 2 cut(s) 14, 280
BsiEI CGRYCG 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 148
BslFI GGGAC 2 cut(s) 44, 77
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 44, 77
BsmI GAATGC 1 cut(s) 304
Bso31I GGTCTC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 116
Bsp143I GATC 3 cut(s) 11, 277, 281
Bsp19I CCATGG 3 cut(s) 25, 134, 206
BspACI CCGC 1 cut(s) 302
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 2 cut(s) 14, 280
BspHI TCATGA 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 242
BspTI CTTAAG 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 15
BsrI ACTGG 1 cut(s) 181
BssECI CCNNGG 3 cut(s) 25, 134, 206
BssMI GATC 3 cut(s) 11, 277, 281
BssT1I CCWWGG 3 cut(s) 25, 134, 206
Bst6I CTCTTC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 234
BstDSI CCRYGG 3 cut(s) 25, 134, 206
BstF5I GGATG 1 cut(s) 157
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 14, 280, 284
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 3 cut(s) 11, 277, 281
BstMCI CGRYCG 1 cut(s) 284
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 147
BstXI CCANNNNNNTGG 2 cut(s) 135, 176
Bsu15I ATCGAT 2 cut(s) 14, 280
BsuTUI ATCGAT 2 cut(s) 14, 280
BtgI CCRYGG 3 cut(s) 25, 134, 206
BtsCI GGATG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 242
BveI ACCTGC 1 cut(s) 133
CciI TCATGA 1 cut(s) 55
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 2 cut(s) 14, 280
Csp6I GTAC 2 cut(s) 166, 243
CviAII CATG 7 cut(s) 26, 34, 56, 98, 135, 207, 247
CviJI RGCY 2 cut(s) 112, 233
CviKI_1 RGCY 2 cut(s) 112, 233
CviQI GTAC 2 cut(s) 166, 243
DdeI CTNAG 1 cut(s) 234
DpnI GATC 3 cut(s) 13, 279, 283
DpnII GATC 3 cut(s) 11, 277, 281
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Eco130I CCWWGG 3 cut(s) 25, 134, 206
Eco31I GGTCTC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 145
Eco47III AGCGCT 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 116
EcoT14I CCWWGG 3 cut(s) 25, 134, 206
ErhI CCWWGG 3 cut(s) 25, 134, 206
FaeI CATG 7 cut(s) 29, 37, 59, 101, 138, 210, 250
FaqI GGGAC 2 cut(s) 44, 77
FatI CATG 7 cut(s) 25, 33, 55, 97, 134, 206, 246
FokI GGATG 1 cut(s) 164
GlaI GCGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 83
HapII CCGG 1 cut(s) 148
HhaI GCGC 1 cut(s) 82
Hin1II CATG 7 cut(s) 29, 37, 59, 101, 138, 210, 250
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HpaII CCGG 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 3 cut(s) 6, 56, 118
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 2 cut(s) 151, 258
HpyCH4V TGCA 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 234
Hsp92II CATG 7 cut(s) 29, 37, 59, 101, 138, 210, 250
HspAI GCGC 1 cut(s) 80
KpnI GGTACC 1 cut(s) 246
Kzo9I GATC 3 cut(s) 11, 277, 281
LmnI GCTCC 3 cut(s) 77, 117, 139
LpnPI CCDG 3 cut(s) 128, 161, 162
MalI GATC 3 cut(s) 13, 279, 283
MboI GATC 3 cut(s) 11, 277, 281
MboII GAAGA 2 cut(s) 21, 283
MluCI AATT 2 cut(s) 47, 183
MnlI CCTC 3 cut(s) 49, 125, 267
MseI TTAA 1 cut(s) 84
MspCI CTTAAG 1 cut(s) 83
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 149
NcoI CCATGG 3 cut(s) 25, 134, 206
NdeII GATC 3 cut(s) 11, 277, 281
NlaIII CATG 7 cut(s) 29, 37, 59, 101, 138, 210, 250
NlaIV GGNNCC 1 cut(s) 244
NspI RCATGY 1 cut(s) 37
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 1 cut(s) 55
PciI ACATGT 1 cut(s) 33
PctI GAATGC 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 147
Ple19I CGATCG 1 cut(s) 284
PscI ACATGT 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 145
PvuI CGATCG 1 cut(s) 284
RsaI GTAC 2 cut(s) 167, 244
RsaNI GTAC 2 cut(s) 166, 243
SaqAI TTAA 1 cut(s) 84
Sau3AI GATC 3 cut(s) 11, 277, 281
Sau96I GGNCC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 149
SetI ASST 4 cut(s) 114, 147, 162, 223
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 145
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 83, 116
SmoI CTYRAG 2 cut(s) 83, 116
Sse9I AATT 2 cut(s) 47, 183
SsiI CCGC 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 3 cut(s) 25, 134, 206
TaqI TCGA 3 cut(s) 14, 117, 280
TasI AATT 2 cut(s) 47, 183
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 242
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 1 cut(s) 242
Vha464I CTTAAG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 1 cut(s) 47
XceI RCATGY 1 cut(s) 37
XhoI CTCGAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.