Rh2DG397000

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000798
Physical Location & Seq
Reverse (-)
57758213 .. 57773156
14944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG397000.1

Sequence Viewer

Length: 6885 bp
ATGTGGAACAACGGCCAGATTGTGCCGCCAGGCACTGGTGGCTCGTCGATTCCGCCACCACCCGCGGCTCAGCCCTCTTATACGGTGTTGCCGCCTCCGGCAGATGCCGAGGCCGTGCTCGAAGAGAAGGCTCGTAAATGGCAGCAACTCAATTCCAAGCGGTACAGCGACAAGCGCAAGTTTGGCTTCGTTGAGACGCAGAAGGAGGACATGCCTCCTGAGCATGTCAGGAAGATCATTAGAGACCATGGGGACATGTCCTCGAAGAAATTCCGTCACGATAAACGCGTGTATCTAGGAGCACTTAAATTTGTTCCGCATGCAGTTTACAAGCTTCTCGAGAATATGCCAATGCCCTGGGAGCAGGTCCGTGATGTGAAGGTTCTGTACCATATAACTGGGGCAATTACATTTGTGAATGAGATACCTTGGGTTGTTGAACCTATATATCTGGCTCAGTGGGGTTCAATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGACATTTTAAAAGAATGCGGTTTCCACCTTTTGATGATGAGGAACCTCCATTAGATTATGCTGATAATCTGTTAGATGTTGATCCTCTAGAGCCAATTCAACTGGAGTTGGATGAAGACGAAGATTCTGCTGTTTATACTTGGTTTTATGACCATAAACCTCTAGTCAAGACAAAACTCATTAACGGTCCAAGTTACCGGAAATGGCATCTCTCCCTTCCAATCATGGCAACTCTCCACCGGCTTGCAGGACAGCTACTATCTGATTTGATAGACCGCAATTATTTCTACTTGTTTGACATGGAGTCTTTCTTTACAGCGAAAGCCCTCAATATGTGCATTCCAGGTGGGCCTAAGTTTGAACCTTTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATTAACAAGCTGATTATTCGGTCACCTCTCAGAACAGAGTACAGAATTGCGTTCCCTCATCTCTACAACAATAGACCCAGGAAGGTAAAGCTCTGTGTGTATCACACACCCATGGTTATGTACATAAAAACTGAGGATCCTGATCTACCTGCATTTTATTATGATCCCTTGATACACCCAATCCCCAGCACAAACAAGGAGCGACGTGAAAAGAAGATTGATGAGGATGATGACACTTTCATACTGCCAGAAGGGGTAGAACCATTTCTGAGTGATACTCAGCTTTATACTGACACAACAGCAGCTGGAGTTTCCCTCTTGTTTGCACCACGCCCCTTTAACATGAGATCTGGGCGGACTCGCCGTGCAGAAGATATACCCCTTGTGTCAGAGTGGTATAAAGAGCATTGTCCTCCGTCCTATCCAGTGAAAGTGCGGGTCAGCTATCAGAAATTGTTAAAATGTTTTGTGTTGAACGAGTTGCATCATAGACCACCCAAGGCTCAAAAGAAGAAGCACTTGTTTCGATCACTTCAAGCTACTAAGTTCTTCCAAACTACAGAACTTGATTGGGCTGAAGCGGGTCTTCAAGTCTGCAAGCAGGGTTATAACATGCTAAATCTCTTGATCCACAGGAAAAATTTGAATTACCTTCACCTTGATTATAATTTCAATCTGAAGCCTGTGAAAACCCTGACAACTAAAGAGCGTAAAAAGTCCCGGTTTGGTAATGCTTTTCATCTTTGCCGTGAGATCTTGCGGTTGACAAAGCTGGTAGTTGATGCCAACATCCAGTTCCGTTTGGGTAATGTCGATGCCTTTCAATTGGCTGATGGCCTGCAGTACACTTTCTCTCATGTTGGCCAGTTGACTGGTATGTATCGTTACAAATACAGGTTGATGCGTCAGATTAGAATGTGCAAAGATCTGAAGCACTTGATTTACTACCGTTTCAACACGGGACCAGTTGGGAAAGGGCCTGGATGTGGATTCTGGGCGCCGATGTGGAGGGTTTGGTTGTTCTTCCTTCGTGGCATAGTTCCTCTTTTGGAACGGTGGTTGGGAAATTTACTTGCACGGCAATTTGAAGGGCGCCATTCAAAAGGAGTGGCAAAAACTGTAACTAAGCAGCGTGTGGAAAGCCATTTTGATTTGGAGCTTCGAGCTGCTGTCATGCATGATGTTCTTGATGCGATGCCTGAGGGAATCAAGCAAAATAAGGCCAGAACTATATTGCAACATCTCAGTGAGGCATGGCGTTGCTGGAAAGCAAATATTCCTTGGAAGGTACCTGGTTTGCCAGTTCCCATTGAGAATATGATTCTTCGTTATGTCAAGTCAAAGGCAGACTGGTGGACAAATGTGGCTCATTACAATCGTGAACGTATAAGAAGAGGTGCTACAGTAGATAAGACAGTTTGTCGGAAGAATCTTGGAAGATTAACACGCCTTTGGCTGAAGGCAGAGCAGGAACGACAGCACAATTATCTCAAAGATGGCCCATATGTTACACCAGAGGAGGCAGTTGCAATTTACACGACAACTGTGCATTGGTTGGAGTCAAGAAAGTTCAGCCCCATCCCATTTCCTCCGTTGTCGTACAAGCATGATACCAAGCTACTCATCCTTGCTCTGGAGAGGTTGAAGGAATCATATAGTGTTGCTGTGAGACTGAATCAGCTTCAAAGAGAAGAGCTTGGTCTTATTGAACAAGCCTATGATAACCCGCATGAAGCATTGTCACGAATTAAGCGTCACTTACTCACTCAGCGTGCCTTCAAAGAAGTTGGCATAGAGTTCATGGATTTGTACAGCTATCTCATTCCAGTCTATGAGATTGAACCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGGTGACAAACGTCATCTCTTCCCGAACTGGATCAAGCCTGCAGATTCGGAGCCACCTCCACTCTTGGTTTATAAATGGTGTCAGGGAATAAACAATTTACAGAGTATCTGGGACACGAGTGACGGGCAGTGTGTTGTTATGCTTCAGACCAAGTTTGAGAAGTTCTTTGAAAAGATTGACTTGACAATGCTAAATAGGCTTCTACGTCTAGTTCTTGATCATAATATCGCTGATTATGTCACTGCGAAGAACAATGTGGTATTGTCCTACAAGGATATGAGTCATACAAATTCGTATGGTCTTATTCGTGGTCTTCAGTTTGCATCCTTTGTTGTACAGTATTATGGGCTTGTGTTGGATCTATTGCTTCTTGGATTGACTCGAGCCAGTGAGATTGCTGGACCACCCCAGATGCCAAATGAGTTCATCACTTATTGGGACACAAAGGTCGAGACCCGGCATCCTATCAGGCTGTACTCTCGATACATAGACAGGGTGCACATCTTGTTCCGCTTCACTCATGAGGAAGCTCGGGATCTTATCCAGCGATATCTCACTGAACATCCTGATCCTAATAATGAGAATATGGTGGGATATAACAACAAGAAGTGCTGGCCAAGAGATGCCAGAATGAGGCTCATGAAACACGATGTCAATCTTGGAAGGAGTGTTTTCTGGGATATGAAAAACCGTCTCCCTCGAAGCATCACAACTTTAGAGTGGGAGAACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTCTTCAGCATGTGTGGGTTTGAGGTTCGGATACTTCCCAAGATAAGAATGAGCCAAGAGGCATTCAGCAACACAAGAGATGGAGTTTGGAATTTGCAGAATGAACAGACAAAGGAAAGGACTGCAGTTGCTTTCTTACGTGTTGATGATGAACACATGAAAGTATTTGAGAATCGTGTGAGGCAGATTCTTATGTCATCAGGGTCTACCACATTTACAAAGATTGTCAACAAATGGAATACAGCTCTGATTGGTCTTATGACTTACTTCCGGGAAGCAACTGTACATACGCAAGAACTGTTAGATTTACTAGTCAAGTGCGAAAACAAAATTCAGACCCGCATTAAGATTGGATTAAATTCAAAGATGCCTAGTAGGTTTCCACCTGTCATCTTTTACACACCAAAAGAAATTGGAGGACTTGGAATGTTATCTATGGGTCACATATTGATTCCCCAAAGTGATCTCAGATACAGTCAGCAGACAGATGTTGGTGTGACACACTTTAGGAGTGGGATGAGTCATGAAGAGGACCAGCTGATTCCAAATCTGTACCGTTACATACAACCTTGGGAGAGTGAATTCATTGATTCCCAGCGGGTTTGGGCAGAATATGCTCTGAAAAGGCAGGAGGCTCAAGCACAAAACAGGCGCCTAACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATTCCACGCATAAATACTTTGTTCCAGAAAGATCGGCACACTTTAGCATATGACAAAGGGTGGAGAGTTCGTACTGATTTTAAGCAGTACCAGGTCTTGAAACAAAATCCTTTTTGGTGGACACACCAAAGGCATGACGGAAAACTTTGGAACTTGAACAACTACAGAACTGATGTTATTCAAGCTCTTGGAGGTGTGGAGGGAATCTTGGAGCATACCTTATTTAAAGGAACATACTTCCCTACCTGGGAGGGTCTCTTCTGGGAAAAAGCATCTGGTTTTGAGGAATCGATGAAGTACAAGAAGCTGACTAATGCACAGAGATCTGGGCTCAACCAAATTCCCAATCGTAGGTTCACGCTTTGGTGGTCGCCGACCATTAATCGGGCTAATGTATATGTCGGTTTCCAAGTGCAGTTAGATCTTACTGGGATCTTTATGCATGGGAAAATTCCAACCTTGAAGATATCCTTGATTCAAATATTCCGTGCACATTTGTGGCAAAAGATCCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAGGAGTTAGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACGATTCATCCCAGGAAGAGTTACAAAATGAACAGTTCTTGTGCTGACATTCTACTCTTTGCTGCCCATAGATGGCCAATGTCAAAACCTAGTCTTGTGGCGGAGCCAAAGGATGTGTTTGATCAGAAAGCAAGCAACAAGTACTGGATAGATGTACAGCTCCGATGGGGAGATTATGATTCCCACGATATTGAACGTTACACCCGAGCCAAGTTTATGGATTATACAACAGACAACATGTCTATCTATCCCTCCCCCACAGGGGTGATGATTGGTCTTGATCTGGCGTATAATTTACATTCTGCTTTTGGTAATTGGTTTCCTGGGTCCAAACCACTGCTTCAGCAGGCAATGAACAAAATAATGAAGTCAAATCCAGCTTTGTACGTCTTGCGGGAGCGGATACGGAAAGGATTGCAGCTTTACTCTTCAGAGCCTACAGAACCTTACTTGTCATCCCAGAACTATGGGGAGATTTTCAGCAATCAAATTATTTGGTTTGTTGATGATACCAATGTGTATCGTGTTACAATCCACAAGACATTTGAAGGAAATCTCACTACAAAACCAATTAATGGTGCGATTTTCATATTCAATCCAAGGACTGGGCAGCTGTTCCTGAAGGTCATCCACACAAGTGTATGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCCAAGTGGAAAACCGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTACCAGTTGAAGAGCAGCCAAAACAAATTATTGTGACTCGAAAGGGAATGCTTGATCCGCTGGAGGTGCACTTGTTGGATTTCCCTAACATAGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTCCAGGCTTGTCTTAAGATTGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAGCCACAGATGGTTCTTTTCAACATCTATGATGATTGGTTGAAGAGTATATCATCATATACTGCATTCTCTCGGCTCATTTTGATTCTTCGTGCACTTCATGTGAACAATGAGAAGGCAAAGATGTTACTGAAACCTGATACAACGGTCATTACTGAGCCACACCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTAAGGGATCTGATTCTCTCAGATTATGCAAAAAAGAACAACGTGAACACATCAGCTCTGACACAATCTGAGATTCGTGATATCATTCTTGGAGCTGAGATTACTCCTCCTTCACAGCAAAGGCAACAAATTGCAGAGATTGAAAAGCAGCATAAAGAAGCAAGTCAGCTGACAGCAGTTACTACTAGGACGACGAATGTGCATGGTGATGAACTCATTGTCACCACTACTAGTCCTTATGAGCAAGCTGCATTTGGGTCCAAGACCGATTGGCGTGTGAGAGCAATATCGGCTACCAATCTCTACCTTCGTGTCAATCATATATATGTGAATTCAGAGGATGTGAAGGAAACGGGTTACACTTATATCATGCCAAAGAACATATTGAAGAAGTTCATCTGTGTTGCGGATCTGAGGACACAAATTGCTGGTTACTTGTACGGTGTAAGCCCACCTGACAACCCTCAGGTTAAGGAAATTCGCTGCATTGCCATGCCACCGCAGTGGGGAACCCACCAGCAAGTTAATCTTCCAACTGCTCTTCCCGAGCATGATTTCCTCAATGATCTGGAACCTTTGGGATGGATGCACACACAACCAAACGAACTTCCTCAGCTTTCTCCTCAGGATCTAACCTCTCATGCTAAGATCTTGGAAAGCACCAAGCAATGGGATGGGGAGAAGTGCATCATTTTAACCTGCAGTTTCACTCCTGGTTCCTGCTCGTTAACTGCATACAAACTCACTCCATCTGGTTATGAGTGGGGACGGGTCAACAAAGACACAGGAAGCAATCCACACGGTTACCTCCCAACTCATTACGAGAAGGTCCAAATGCTTCTCAGTGATCGGTTCCTTGGTTTCTATATGGTAAGATCATTATATCACATGTTCAGTTTTGCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2294

Amino Acids

268.01

Weight (kDa)

9.01

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 219 4.1e-85 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 405 - 810 1e-235 PROCN (NUC071) domain
RRM_4 PF10598 995 - 1085 1.4e-46 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1247 - 1382 4e-75 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1452 - 1610 8.4e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1770 - 1999 2.6e-129 PRP8 domain IV core
JAB PF01398 2110 - 2209 1.1e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2222 - 2284 2.3e-22 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1530, 1587, 2886
AasI GACNNNNNNGTC 2 cut(s) 3260, 5523
Acc36I ACCTGC 3 cut(s) 355, 1078, 6683
Acc65I GGTACC 1 cut(s) 2209
AccB1I GGYRCC 4 cut(s) 1918, 2013, 2209, 4233
AccB7I CCANNNNNTGG 4 cut(s) 35, 5441, 5471, 6645
AccBSI CCGCTC 1 cut(s) 5266
AccI GTMKAC 2 cut(s) 505, 3788
AccII CGCG 2 cut(s) 65, 288
AclI AACGTT 1 cut(s) 5062
AcoI YGGCCR 5 cut(s) 13, 1783, 3428, 4940, 5538
AcyI GRCGYC 3 cut(s) 1919, 2014, 4234
AflII CTTAAG 1 cut(s) 5720
AflIII ACRYGT 5 cut(s) 255, 286, 3721, 5103, 6864
AhdI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
AhlI ACTAGT 2 cut(s) 3892, 6206
AjiI CACGTC 1 cut(s) 1127
AjuI GAANNNNNNNTTGG 4 cut(s) 1700, 1732, 4613, 4645
AleI CACNNNNGTG 4 cut(s) 4771, 5129, 5502, 6478
Alw21I GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5655, 5857
Alw26I GTCTC 7 cut(s) 188, 237, 2584, 3260, 3512, 4535, 4844
Alw44I GTGCAC 4 cut(s) 3311, 4764, 5651, 5853
AlwNI CAGNNNCTG 2 cut(s) 35, 1226
Ama87I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
ApaLI GTGCAC 4 cut(s) 3311, 4764, 5651, 5853
ArsI GACNNNNNNTTYG 2 cut(s) 2659, 2691
AseI ATTAAT 2 cut(s) 4656, 5439
Asp700I GAANNNNTTC 4 cut(s) 269, 1185, 4869, 6368
Asp718I GGTACC 1 cut(s) 2209
AspLEI GCGC 4 cut(s) 177, 1921, 2016, 4236
AsuC2I CCSGG 3 cut(s) 1642, 3271, 3854
AsuHPI GGTGA 7 cut(s) 936, 1568, 2828, 5143, 5750, 6190, 6194
AvaI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
AvaII GGWCC 8 cut(s) 367, 692, 1883, 3215, 4114, 5193, 6234, 6805
AxyI CCTNAGG 3 cut(s) 2121, 6441, 6600
BaeGI GKGCMC 4 cut(s) 3315, 4768, 5655, 5857
BaeI ACNNNNGTAYC 8 cut(s) 275, 308, 857, 890, 5367, 5400, 5892, 5925
BalI TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
BamHI GGATCC 1 cut(s) 1057
BanI GGYRCC 4 cut(s) 1918, 2013, 2209, 4233
BanII GRGCYC 1 cut(s) 4608
BarI GAAGNNNNNNTAC 6 cut(s) 371, 403, 2305, 2337, 3849, 3881
BauI CACGAG 1 cut(s) 2929
BbsI GAAGAC 3 cut(s) 627, 1499, 3119
Bbv12I GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5655, 5857
BbvCI CCTCAGC 1 cut(s) 6588
BceAI ACGGC 5 cut(s) 28, 98, 1269, 1653, 2015
BcgI CGANNNNNNTGC 8 cut(s) 614, 648, 1427, 1461, 2449, 2483, 6157, 6191
BciVI GTATCC 2 cut(s) 3606, 5262
BclI TGATCA 4 cut(s) 2794, 3031, 4987, 5950
BcnI CCSGG 3 cut(s) 1642, 3271, 3854
BcoDI GTCTC 7 cut(s) 188, 237, 2584, 3260, 3512, 4535, 4844
BcuI ACTAGT 2 cut(s) 3892, 6206
BfaI CTAG 9 cut(s) 296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
BfmI CTRYAG 9 cut(s) 1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
BfoI RGCGCY 3 cut(s) 1922, 2017, 4237
BfrI CTTAAG 1 cut(s) 5720
BfuAI ACCTGC 3 cut(s) 355, 1078, 6683
BfuI GTATCC 2 cut(s) 3606, 5262
BglII AGATCT 6 cut(s) 1268, 1674, 1846, 4598, 4696, 6624
BlpI GCTNAGC 1 cut(s) 69
BmcAI AGTACT 1 cut(s) 5009
Bme18I GGWCC 8 cut(s) 367, 692, 1883, 3215, 4114, 5193, 6234, 6805
BmeRI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
BmeT110I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
BmgBI CACGTC 1 cut(s) 1127
BmrI ACTGGG 3 cut(s) 408, 4713, 5481
BmuI ACTGGG 3 cut(s) 408, 4713, 5481
BoxI GACNNNNGTC 1 cut(s) 2823
BpiI GAAGAC 3 cut(s) 627, 1499, 3119
BplI GAGNNNNNCTC 4 cut(s) 1183, 1215, 1221, 1253
BpmI CTGGAG 4 cut(s) 629, 1248, 2576, 5666
Bpu10I CCTNAGC 2 cut(s) 219, 6588
Bpu1102I GCTNAGC 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 2789, 4203
BpuMI CCSGG 3 cut(s) 1642, 3271, 3854
Bsa29I ATCGAT 1 cut(s) 4565
BsaAI YACGTR 1 cut(s) 3722
BsaBI GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BsaHI GRCGYC 3 cut(s) 1919, 2014, 4234
BsaI GGTCTC 3 cut(s) 237, 3260, 4535
BsaWI WCCGGW 1 cut(s) 702
BsaXI ACNNNNNCTCC 2 cut(s) 4330, 4360
Bse118I RCCGGY 1 cut(s) 744
Bse21I CCTNAGG 3 cut(s) 2121, 6441, 6600
Bse3DI GCAATG 3 cut(s) 5223, 6462, 6650
Bse8I GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BseCI ATCGAT 1 cut(s) 4565
BseJI GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BseMI GCAATG 3 cut(s) 5223, 6462, 6650
BseRI GAGGAG 3 cut(s) 2453, 6072, 6588
BseSI GKGCMC 4 cut(s) 3315, 4768, 5655, 5857
BseYI CCCAGC 2 cut(s) 1106, 4176
BsgI GTGCAG 4 cut(s) 1308, 4709, 4877, 5553
Bsh1236I CGCG 2 cut(s) 65, 288
BshNI GGYRCC 4 cut(s) 1918, 2013, 2209, 4233
BshVI ATCGAT 1 cut(s) 4565
BsiHKAI GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5655, 5857
BsiHKCI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
BsiSI CCGG 6 cut(s) 98, 703, 745, 1642, 3271, 3853
BslFI GGGAC 6 cut(s) 266, 1624, 1896, 2939, 3266, 6756
BsmAI GTCTC 7 cut(s) 188, 237, 2584, 3260, 3512, 4535, 4844
BsmBI CGTCTC 2 cut(s) 188, 3512
BsmFI GGGAC 6 cut(s) 266, 1624, 1896, 2939, 3266, 6756
BsmI GAATGC 5 cut(s) 525, 843, 3644, 5637, 5825
Bso31I GGTCTC 3 cut(s) 237, 3260, 4535
BsoBI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
Bsp1286I GDGCHC 7 cut(s) 120, 304, 3315, 4608, 4768, 5655, 5857
Bsp1407I TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
Bsp1720I GCTNAGC 1 cut(s) 69
Bsp19I CCATGG 2 cut(s) 247, 1032
BspDI ATCGAT 1 cut(s) 4565
BspFNI CGCG 2 cut(s) 65, 288
BspHI TCATGA 3 cut(s) 3334, 3453, 4105
BspMAI CTGCAG 5 cut(s) 1764, 2857, 3709, 5700, 6680
BspMI ACCTGC 3 cut(s) 355, 1078, 6683
BspQI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
BspT107I GGYRCC 4 cut(s) 1918, 2013, 2209, 4233
BspTI CTTAAG 1 cut(s) 5720
BspTNI GGTCTC 3 cut(s) 237, 3260, 4535
BsrBI CCGCTC 1 cut(s) 5266
BsrDI GCAATG 3 cut(s) 5223, 6462, 6650
BsrFI RCCGGY 1 cut(s) 744
BsrGI TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
BssAI RCCGGY 1 cut(s) 744
BssNI GRCGYC 3 cut(s) 1919, 2014, 4234
BssSI CACGAG 1 cut(s) 2929
BssT1I CCWWGG 8 cut(s) 247, 428, 1032, 1419, 2201, 4151, 5465, 6832
Bst2BI CACGAG 1 cut(s) 2929
BstACI GRCGYC 3 cut(s) 1919, 2014, 4234
BstAFI CTTAAG 1 cut(s) 5720
BstAPI GCANNNNNTGC 1 cut(s) 4196
BstAUI TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
BstBAI YACGTR 1 cut(s) 3722
BstDSI CCRYGG 3 cut(s) 63, 247, 1032
BstEII GGTNACC 2 cut(s) 942, 6779
BstFNI CGCG 2 cut(s) 65, 288
BstH2I RGCGCY 3 cut(s) 1922, 2017, 4237
BstHHI GCGC 4 cut(s) 177, 1921, 2016, 4236
BstMAI GTCTC 7 cut(s) 188, 237, 2584, 3260, 3512, 4535, 4844
BstNSI RCATGY 8 cut(s) 214, 227, 259, 323, 1537, 3595, 5107, 6868
BstPAI GACNNNNGTC 1 cut(s) 2823
BstPI GGTNACC 2 cut(s) 942, 6779
BstSFI CTRYAG 9 cut(s) 1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
BstSLI GKGCMC 4 cut(s) 3315, 4768, 5655, 5857
BstUI CGCG 2 cut(s) 65, 288
BstV2I GAAGAC 3 cut(s) 627, 1499, 3119
BstXI CCANNNNNNTGG 6 cut(s) 357, 398, 1793, 5083, 5333, 6480
Bsu15I ATCGAT 1 cut(s) 4565
Bsu36I CCTNAGG 3 cut(s) 2121, 6441, 6600
BsuI GTATCC 2 cut(s) 3606, 5262
BsuTUI ATCGAT 1 cut(s) 4565
BtgI CCRYGG 3 cut(s) 63, 247, 1032
BtgZI GCGATG 1 cut(s) 2129
BtrI CACGTC 1 cut(s) 1127
BtsI GCAGTG 4 cut(s) 2948, 3054, 5201, 6485
BveI ACCTGC 3 cut(s) 355, 1078, 6683
CaiI CAGNNNCTG 2 cut(s) 35, 1226
CciI TCATGA 3 cut(s) 3334, 3453, 4105
CfoI GCGC 4 cut(s) 177, 1921, 2016, 4236
Cfr10I RCCGGY 1 cut(s) 744
Cfr42I CCGCGG 1 cut(s) 66
ClaI ATCGAT 1 cut(s) 4565
CseI GACGC 4 cut(s) 205, 1814, 2663, 5513
CsiI ACCWGGT 2 cut(s) 2212, 4365
CspCI CAANNNNNGTGG 2 cut(s) 2010, 2045
DinI GGCGCC 3 cut(s) 1920, 2015, 4235
DraI TTTAAA 2 cut(s) 514, 4501
DrdI GACNNNNNNGTC 2 cut(s) 3260, 5523
DriI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
DseDI GACNNNNNNGTC 2 cut(s) 3260, 5523
EaeI YGGCCR 5 cut(s) 13, 1783, 3428, 4940, 5538
Eam1105I GACNNNNNGTC 3 cut(s) 808, 2340, 5104
EciI GGCGGA 3 cut(s) 42, 1291, 4982
Eco130I CCWWGG 8 cut(s) 247, 428, 1032, 1419, 2201, 4151, 5465, 6832
Eco24I GRGCYC 1 cut(s) 4608
Eco31I GGTCTC 3 cut(s) 237, 3260, 4535
Eco32I GATATC 3 cut(s) 3365, 4743, 6058
Eco47I GGWCC 8 cut(s) 367, 692, 1883, 3215, 4114, 5193, 6234, 6805
Eco81I CCTNAGG 3 cut(s) 2121, 6441, 6600
Eco88I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
Eco91I GGTNACC 2 cut(s) 942, 6779
EcoO109I RGGNCCY 1 cut(s) 1898
EcoO65I GGTNACC 2 cut(s) 942, 6779
EcoRI GAATTC 3 cut(s) 4163, 4275, 6307
EcoRV GATATC 3 cut(s) 3365, 4743, 6058
EcoT14I CCWWGG 8 cut(s) 247, 428, 1032, 1419, 2201, 4151, 5465, 6832
EcoT22I ATGCAT 3 cut(s) 2100, 2788, 4719
EcoT38I GRGCYC 1 cut(s) 4608
EgeI GGCGCC 3 cut(s) 1920, 2015, 4235
EheI GGCGCC 3 cut(s) 1920, 2015, 4235
ErhI CCWWGG 8 cut(s) 247, 428, 1032, 1419, 2201, 4151, 5465, 6832
Esp3I CGTCTC 2 cut(s) 188, 3512
FaqI GGGAC 6 cut(s) 266, 1624, 1896, 2939, 3266, 6756
FauI CCCGC 7 cut(s) 70, 1350, 1495, 2655, 3929, 4173, 5253
FauNDI CATATG 3 cut(s) 2425, 4324, 6881
FbaI TGATCA 4 cut(s) 2794, 3031, 4987, 5950
FblI GTMKAC 2 cut(s) 505, 3788
FriOI GRGCYC 1 cut(s) 4608
FspBI CTAG 9 cut(s) 296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
GlaI GCGC 4 cut(s) 176, 1920, 2015, 4235
GsaI CCCAGC 2 cut(s) 1110, 4180
GsuI CTGGAG 4 cut(s) 629, 1248, 2576, 5666
HaeII RGCGCY 3 cut(s) 1922, 2017, 4237
HapII CCGG 6 cut(s) 98, 703, 745, 1642, 3271, 3853
HgaI GACGC 4 cut(s) 205, 1814, 2663, 5513
HhaI GCGC 4 cut(s) 177, 1921, 2016, 4236
Hin1I GRCGYC 3 cut(s) 1919, 2014, 4234
Hin6I GCGC 4 cut(s) 175, 1919, 2014, 4234
HinP1I GCGC 4 cut(s) 175, 1919, 2014, 4234
HincII GTYRAC 6 cut(s) 506, 1686, 1791, 3811, 6705, 6751
HindII GTYRAC 6 cut(s) 506, 1686, 1791, 3811, 6705, 6751
HindIII AAGCTT 1 cut(s) 332
HpaI GTTAAC 1 cut(s) 6705
HpaII CCGG 6 cut(s) 98, 703, 745, 1642, 3271, 3853
HphI GGTGA 7 cut(s) 936, 1568, 2828, 5143, 5750, 6190, 6194
Hpy99I CGWCG 4 cut(s) 49, 507, 1128, 6172
HpyCH4IV ACGT 8 cut(s) 1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
HpySE526I ACGT 8 cut(s) 1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
Hsp92I GRCGYC 3 cut(s) 1919, 2014, 4234
HspAI GCGC 4 cut(s) 175, 1919, 2014, 4234
KasI GGCGCC 3 cut(s) 1918, 2013, 4233
KpnI GGTACC 1 cut(s) 2213
Ksp22I TGATCA 4 cut(s) 2794, 3031, 4987, 5950
KspAI GTTAAC 1 cut(s) 6705
KspI CCGCGG 1 cut(s) 66
LguI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
MabI ACCWGGT 2 cut(s) 2212, 4365
MaeI CTAG 9 cut(s) 296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
MaeII ACGT 8 cut(s) 1126, 2305, 2824, 3019, 3721, 5062, 5253, 6018
MbiI CCGCTC 1 cut(s) 5266
MfeI CAATTG 1 cut(s) 1745
MhlI GDGCHC 7 cut(s) 120, 304, 3315, 4608, 4768, 5655, 5857
MlsI TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
MluI ACGCGT 1 cut(s) 286
MluNI TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
Mly113I GGCGCC 3 cut(s) 1919, 2014, 4234
MlyI GAGTC 7 cut(s) 818, 1273, 2489, 3103, 3187, 4111, 5614
MmeI TCCRAC 7 cut(s) 594, 2324, 2457, 3150, 4754, 5640, 6533
Mox20I TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
Mph1103I ATGCAT 3 cut(s) 2100, 2788, 4719
MroXI GAANNNNTTC 4 cut(s) 269, 1185, 4869, 6368
MscI TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
Msp20I TGGCCA 4 cut(s) 1785, 3430, 4942, 5540
MspA1I CMGCKG 7 cut(s) 65, 1226, 4120, 4180, 5479, 5644, 6145
MspCI CTTAAG 1 cut(s) 5720
MspI CCGG 6 cut(s) 98, 703, 745, 1642, 3271, 3853
MunI CAATTG 1 cut(s) 1745
Mva1269I GAATGC 5 cut(s) 525, 843, 3644, 5637, 5825
MvnI CGCG 2 cut(s) 65, 288
NarI GGCGCC 3 cut(s) 1919, 2014, 4234
NciI CCSGG 3 cut(s) 1642, 3271, 3854
NcoI CCATGG 2 cut(s) 247, 1032
NdeI CATATG 3 cut(s) 2425, 4324, 6881
NmeAIII GCCGAG 2 cut(s) 133, 5812
NsiI ATGCAT 3 cut(s) 2100, 2788, 4719
NspI RCATGY 8 cut(s) 214, 227, 259, 323, 1537, 3595, 5107, 6868
OliI CACNNNNGTG 4 cut(s) 4771, 5129, 5502, 6478
PaeI GCATGC 1 cut(s) 323
PaeR7I CTCGAG 2 cut(s) 338, 3195
PagI TCATGA 3 cut(s) 3334, 3453, 4105
PasI CCCWGGG 1 cut(s) 357
PciI ACATGT 3 cut(s) 255, 5103, 6864
PciSI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 2671, 5068
PctI GAATGC 5 cut(s) 525, 843, 3644, 5637, 5825
PdmI GAANNNNTTC 4 cut(s) 269, 1185, 4869, 6368
PflMI CCANNNNNTGG 4 cut(s) 35, 5441, 5471, 6645
PfoI TCCNGGA 1 cut(s) 3852
PleI GAGTC 7 cut(s) 817, 1273, 2488, 3102, 3187, 4110, 5614
PluTI GGCGCC 3 cut(s) 1922, 2017, 4237
PpsI GAGTC 7 cut(s) 817, 1273, 2488, 3102, 3187, 4110, 5614
Ppu21I YACGTR 1 cut(s) 3722
PscI ACATGT 3 cut(s) 255, 5103, 6864
PshAI GACNNNNGTC 1 cut(s) 2823
PshBI ATTAAT 2 cut(s) 4656, 5439
PsiI TTATAA 3 cut(s) 1530, 1587, 2886
Psp1406I AACGTT 1 cut(s) 5062
PspEI GGTNACC 2 cut(s) 942, 6779
PspFI CCCAGC 2 cut(s) 1106, 4176
PspXI VCTCGAGB 1 cut(s) 3195
PstI CTGCAG 5 cut(s) 1764, 2857, 3709, 5700, 6680
PstNI CAGNNNCTG 2 cut(s) 35, 1226
PvuII CAGCTG 4 cut(s) 1226, 4120, 5479, 6145
SacII CCGCGG 1 cut(s) 66
SalI GTCGAC 1 cut(s) 504
SapI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
ScaI AGTACT 1 cut(s) 5009
SchI GAGTC 7 cut(s) 818, 1273, 2489, 3103, 3187, 4111, 5614
SduI GDGCHC 7 cut(s) 120, 304, 3315, 4608, 4768, 5655, 5857
SexAI ACCWGGT 2 cut(s) 2212, 4365
SfcI CTRYAG 9 cut(s) 1479, 1760, 2322, 2853, 3705, 4438, 5304, 5696, 6676
SfoI GGCGCC 3 cut(s) 1920, 2015, 4235
Sfr274I CTCGAG 2 cut(s) 338, 3195
Sfr303I CCGCGG 1 cut(s) 66
SgrBI CCGCGG 1 cut(s) 66
SinI GGWCC 8 cut(s) 367, 692, 1883, 3215, 4114, 5193, 6234, 6805
SlaI CTCGAG 2 cut(s) 338, 3195
SmlI CTYRAG 5 cut(s) 338, 2768, 3195, 4218, 5720
SmoI CTYRAG 5 cut(s) 338, 2768, 3195, 4218, 5720
SpeI ACTAGT 2 cut(s) 3892, 6206
SphI GCATGC 1 cut(s) 323
SspDI GGCGCC 3 cut(s) 1918, 2013, 4233
SspI AATATT 2 cut(s) 2197, 4758
SspMI CTAG 9 cut(s) 296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
StyI CCWWGG 8 cut(s) 247, 428, 1032, 1419, 2201, 4151, 5465, 6832
TaiI ACGT 8 cut(s) 1129, 2308, 2827, 3022, 3724, 5065, 5256, 6021
TaqII GACCGA 2 cut(s) 930, 6257
TauI GCSGC 3 cut(s) 28, 68, 94
TspGWI ACGGA 8 cut(s) 263, 359, 1326, 1709, 2502, 4428, 4751, 5287
Van91I CCANNNNNTGG 4 cut(s) 35, 5441, 5471, 6645
Vha464I CTTAAG 1 cut(s) 5720
VneI GTGCAC 4 cut(s) 3311, 4764, 5651, 5853
VpaK11BI GGWCC 8 cut(s) 367, 692, 1883, 3215, 4114, 5193, 6234, 6805
VspI ATTAAT 2 cut(s) 4656, 5439
XbaI TCTAGA 1 cut(s) 592
XceI RCATGY 8 cut(s) 214, 227, 259, 323, 1537, 3595, 5107, 6868
XcmI CCANNNNNNNNNTGG 1 cut(s) 5928
XhoI CTCGAG 2 cut(s) 338, 3195
XmiI GTMKAC 2 cut(s) 505, 3788
XmnI GAANNNNTTC 4 cut(s) 269, 1185, 4869, 6368
XspI CTAG 9 cut(s) 296, 593, 668, 3023, 3893, 3954, 4956, 6162, 6207
ZrmI AGTACT 1 cut(s) 5009
Zsp2I ATGCAT 3 cut(s) 2100, 2788, 4719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.