MD00G1072300.v1.1

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000798
Physical Location & Seq
Forward (+)
14214266 .. 14224948
10683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1072300.v1.1.491

Sequence Viewer

Length: 7044 bp
ATGTGGAACAACGGCCAGATTGCGCCGCCTGGCACCGGCGGTTCGTCGATTCCGCCACCGCCGGCGGCTCAGCCATCTTACACGGTATTGCCTTCTCCAGCTGAGGCCGAGGCCAAGCTTGAGGAGAAGGCCCGGAAATGGCAGCAACTAAATTCCAAGCGGTATAGCGATAAGCGCAAGTTCGGGTTCGTCGAGTCGCAGAAGGAGGATATGCCTCCTGAACATGTCAGGAAGATCATTAGAGACCATGGGGACATGTCCTCAAAGAAATTTCGTCATGATAAACGAGTGTATCTCGGAGCACTTAAGTTTGTCCCCCATGCAGTTTACAAGCTCCTCGAGAATATGCCAATGCCATGGGAGCAGGTCCGGGATGTGAAGGTTTTGTACCATATAACTGGGGCAATTACATTTGTAAATGAAATACCATGGGTTGTTGAACCCATCTATTTGGCTCAGTGGGGTACGATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGGCATTTCAAAAGAATGCGGTTTCCACCATTTGATGACGAGGAACCTCCATTAGACTATGCTGATAATCTGTTAGATGTTGATCCTCTAGAGCCAATTCAATTGGAGTTGGATGAAGAAGAAGATTCTGCTGTGTATACATGGTTTTATGACCATAAACCTCTTGTCAAAACAAAGCTCATAAACGGTCCAAGTTATAGGAAGTGGCATCTGTCACTTCCGATCATGGCAACTCTTCATCGACTAGCAGGACAGCTGCTTTCTGATCTTATTGACCGCAATTATTTCTACCTGTTCGACATGGAGTCTTTTTTTACAGCCAAAGCACTCAACATGTGCATTCCAGGTGGACCTAAATTTGAACCATTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATCAACAAACTCATTATTCGGTCACCTCTTAGAACAGAGTACAGAATTGCATTCCCTCATCTCTACAACAATAGACCAAGGAAGGTCAAGCTCTGTGTTTATCACACACCCATGGTGATGTACATAAAGGCCGAGGATCCTGATCTACCTGCGTTTTATTATGATCCATTGATACACCCAATACCCAGCACTAACAAGGAGCGGCGTGAGAAGAAAACTTCTGATGAGGAGGAAGATGATATCTTTATGTTGCCGGATGCTGTGGAACCTTTTCTGAAAGATACTCAGCTTTATACTGACACAACAGCTGCCGGAATCTCCCTTTTGTTTGCCCCGCGCCCTTTTAACATGAGATCTGGTCGGACCCGTCGTGCAGAAGATATACCCCTTGTGTCCGAGTGGTATAAGGAACATTGTCCTCCTTCATATCCAGTTAAAGTTCGAGTCAGCTATCAGAAGTTGCTGAAATGTTTTGTATTGAACGAGCTGCATCACAGACCACCCAAAGCTCAAAAGAAGAAACATTTGTTTCGATCTCTCCAAGCTACCAAGTTTTTCCAAACTACAGAACTTGATTGGGCTGAAGCCGGTCTTCAAGTCTGTAAGCAGGGTTATAACATGCTGAATCTCTTGATCCACAGGAAAAATTTGAATTACCTTCACCTTGATTACAATTTTAATCTGAAGCCTGTGAAGACCTTGACAACAAAGGAGCGTAAGAAATCTAGGTTTGGTAATGCTTTTCATCTGTGCCGTGAAATCTTGCGGTTGACAAAGCTTGTAGTTGATGCCAATATCCAGTTCCGTTTGGGTAATGTTGATGCCTTTCAATTGGCTGATGGCTTACAATATACATTTTCTCATGTTGGTCAGTTGACTGGAATGTACCGTTACAAGTACAGGTTGATGAGGCAGATTAGAATGTGCAAAGATTTGAAGCACTTGATTTACTACCGGTTCAATACTGGGCCAGTGGGAAAAGGACCAGGATGTGGGTTCTGGGCACCAATGTGGAGGGTGTGGTTGTTTTTCCTTCGTGGCATAGTTCCTCTTCTGGAACGGTGGCTGGGGAATTTACTAGCACGTCAATTTGAGGGGCGCCATTCAAAAGGAGTGGCAAAGACTGTTACTAAGCAGCGTGTTGAAAGCCATTTTGACTTGGAGCTTCGAGCTGCTGTGATGCATGATGTTCTTGATGCCATGCCTGAGGGTATCAAGCAAAATAAGGCCAGAACTATCTTGCAACATCTCAGTGAGGCATGGCGTTGCTGGAAAGCAAACATTCCTTGGAAGGTTCCTGGTTTGCCAGTTCCCATTGAAAATATGATTCTTCGTTATGTCAAATCAAAGGCAGACTGGTGGACAAATGTTGCTCATTATAATCGTGAACGTATAAGAAGAGGTGCTACTGTTGATAAGACAGTTTGTCGAAAGAATCTTGGAAGATTAACTCGTCTCTGGCTAAAGGCGGAGCAGGAACGACAGCATAATTATTTGAAAGATGGTCCGTATGTTACACCAGAAGAGGCAGTTGCAATTTACACGACAACCGTGCACTGGTTGGAGTCAAGGAAGTTTACACCCATTCCGTTCCCTCCATTGTCGTACAAGCATGATACCAAGCTTCTGATCCTTGCTCTGGAGAGGTTGAAGGAGTCTTATAGTGTGGCTGTAAGATTGAACCAGCTTCAAAGAGAAGAGCTGGGTCTCATTGAACAAGCCTATGATAATCCTCATGAGGCATTGTCACGAATTAAGCGTCACTTGCTCACTCAGCGTGCATTCAAAGAAGTTGGCATAGAGTTCATGGACTTGTATAGCTATCTGATTCCAGTTTATGAGATAGAACCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATTTATGGTATGAGGGTGACAAACGTCATCTCTTTCCCAACTGGATCAAGCCTGCAGATTCAGAGCCACCTCCACTCTTGGTTTATAAGTGGTGTCAGGGAATCAATAATTTGCAGAGCATATGGGATACAAGTGAGGGGCAATGTGTGGTGATGCTTCAAACAAAGTTTGAGAAGTTCTTTGAGAAGATCGACTTGACAATGCTAAATAGGCTCCTGCGGCTTGTTCTTGACCATAATATTGCTGATTATGTCACTGCAAAGAACAATGTGGTGTTGTCCTACAAAGATATGAGTCATACAAATTCGTATGGTCTCATACGTGGTCTTCAGTTTGCATCTTTTGTAGTACAATATTATGGGCTTGTGTTGGATCTGTTACTTCTTGGGCTGACTCGGGCCAGTGAGATTGCCGGTCCACCCCAGATGCCAAATGAGTTCATCACTTATTGGGATACAAAGGTTGAGACTCGGCATCCTATCAGGCTGTATTCTAGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACCCATGAGGAGGCTCGGGACCTTATTCAGCGATATCTTACTGAGCATCCAGACCCCAATAATGAAAATATGGTTGGATATAATAATAAGAAATGCTGGCCAAGAGATGCTAGAATGAGGCTTATGAAACATGATGTCAATCTTGGGAGGAGTGTTTTTTGGGATATGAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAGTGGGAGAACAGCTTTGTGTCTGTTTACAGCAAGGATAATCCAAATTTTCTTTTCAGTATGTCTGGATTTGAAGTTCGGATACTTCCGAAGATAAGAATGAGTCAAGAAGCATTTAGTAACACGAGGGATGGGGTTTGGAATTTGCAGAATGAGCAGACAAAGGAGAGGACTGCTGTTGCTTTCTTACGTGTTGATGATGAACACATGAAGGTGTTTGAGAATCGTGTGAGGCAGATTCTTATGTCTTCAGGGTCTACAACGTTTACAAAAATCGTTAACAAATGGAATACAGCGCTTATTGGTCTAATGACTTACTTCCGTGAAGCAACTGTTCATACACAAGAACTGTTGGATTTGCTCGTCAAATGTGAAAACAAAATTCAGACCCGTATTAAGATTGGGTTGAATTCAAAGATGCCTAGCAGGTTTCCACCTGTCATATTTTACACACCAAAAGAAATTGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAAAGTGACCTCCGTTACAGTCAGCAGACAGATGTTGGTGTGACTCATTTTAGGAGTGGAATGAGTCATGAAGAGGACCAGCTAATCCCAAATCTTTACCGTTACATACAGCCATGGGAGAGTGAATTTATAGATTCTCAACGTGTTTGGGCAGAATATGCACTGAAAAGGCAGGAAGCTCAGGCACAAAATAGGCGTCTGACTCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACTTTGTTCCAGAAGGACCGGCATACTTTAGCATATGACAAAGGATGGAGAGTTCGCACAGATTTTAAGCAGTACCAGGTGTTGAAACAAAATCCTTTCTGGTGGACACACCAGAGGCATGATGGAAAGTTATGGAACTTAAACAACTACCGAACTGATGTGATTCAAGCCCTTGGAGGTGTTGAAGGAATCCTTGAGCATACATTATTCAAAGGAACATACTTTCCTACATGGGAGGGGCTCTTCTGGGAGAAGGCATCTGGTTTTGAGGAGTCGATGAAGTATAAAAAGTTGACCAATGCACAGAGATCTGGGCTCAATCAAATCCCTAATCGTAGATTTACTCTTTGGTGGTCACCTACCATAAATCGGGCCAACGTATATGTCGGTTTCCAAGTGCAGCTAGATTTAACTGGAATATTTATGCACGGGAAGATTCCAACCTTGAAGATATCACTGATTCAGATATTCCGTGCTCACTTGTGGCAGAAGATTCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAAGAGTTGGATGCTTTGGAAATTGAAACTGTGCAGAAAGAAACGATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCTGACATTCTTCTGTTTGCTGCCCATCGATGGCCAATGTCAAAACCTAGTCTTGTAGCTGAGCCCAAGGATGTCTTTGACCAGAAAGTGAGCAATAAGTACTGGATAGATGTACAACTGCGTTGGGGAGATTATGATTCTCACGATATTGAGCGTTACACCCGAGCTAAGTTTATGGACTATACCACTGACAACATGTCCATCTATCCTTCTCCCACAGGGGTGATGATTGGTCTTGATTTGGCCTATAATTTGCATTCTGCATTTGGTAACTGGTTTCCTGGTTCAAAACCCCTGCTTCAACAGGCAATGAACAAGATCATGAAATCAAATCCAGCCTTATATGTCCTGAGGGAGCGAATAAGGAAAGGATTGCAGCTATATTCTTCTGAGCCTACAGAGCCATATTTATCATCCCAAAACTATGGGGAGATCTTCAGCAATCAAATTATTTGGTTTGTTGATGACACCAATGTGTATCGTGTTACAATCCATAAGACATTTGAAGGAAATCTCACTACAAAACCAATCAATGGTGCTATCTTCATATTCAATCCAAGGACAGGGCAGCTATTCCTGAAGGTTATCCACACGAGTGTCTGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCAAAGTGGAAAACTGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTGCCAGTGGAAGAACAACCAAAGCAAATTATTGTGACTCGTAAGGGAATGTTGGATCCCTTAGAGGTTCATTTGCTCGATTTCCCCAACATAGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTCCAAGCTTGTTTGAAGATTGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAACCGCAGATGGTTCTTTTCAATATTTATGATGATTGGTTGAAGAGTATTTCATCATACACTGCATTTTCTCGGCTAATTTTGATTCTTCGTGCTCTTCATGTGAACAATGAGAAGGCAAAGATGTTATTGAAGCCTGATAAAACAGTCGTTACTGAGCCACATCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTTAGGGATCTCATATTGTCAGACTACGCAAAAAAGAACAATGTGAATACATCAGCCCTAACACAATCTGAGATCCGTGACATTATACTTGGAGCTGAGATCACCCCACCTTCGCAACAGAGGCAGCAAATTGCAGAAATAGAAAAACAGGCCAAAGAAGCCAGCCAGCTGACAGCAGTCACAACAAGGACTACAAATGTGCATGGTGATGAACTCATTGTCACCACTACGAGTCCCTATGAGCAAAGTGCATTTGGCTCCAAAACTGATTGGCGTGTGAGAGCAATATCAGCTACAAATCTATATCTCCGTGTCAATCATATATATGTGAATTCAGAAGATATTAAGGAAACGGGTTACACCTATATTATGCCAAAGAACATTTTGAAAAAGTTCATCTGCATTGCGGATCTGAGGACGCAAATAGCTGGTTACTTGTATGGCATAAGCCCACCCGATAACCCTCAGGTCAAGGAAATCCGCTGCATTGCAATGCCTCCACAATGGGGGACCCACCAGCAAGTTCATCTTCCATCCGCTCTTCCTGATCATGATTTCCTCAACGATTTGGAGCCTCTGGGATGGATGCATACACAGCCGAATGAGCTTCCTCAGCTTTCTCCTCAGGATCTCACTTCTCATGCTAAGATTTTAGAGAACACTAAGCAATGGGACGGGGAGAAATGCATCATTTTAACCTGCAGTTTCACTCCAGGTTCCTGCTCTTTAACTGCATACAAGCTTACTCCATCTGGTTATGAATGGGGACGTGTCAATAAAGACACAGGAAGCAATCCTCACGGATACCTTCCCACTCATTACGAGAAGGTGCAGATGCTTCTCAGTGACCGCTTCCTTGGTTTCTATATGATTCCGGACACTGGTCCATGGAACTACAACTTCATGGGGGTGAAGCATACGCCGAGCATGAAGTATGGCATCAAGCTTGGAACACCCAGGGAGTACTATCATGAGGACCACCGGCCGACCCATTACCTTGAGTTCAGCAATCTTGAGGAGGGTGATACAGTGGAAGGAGATCGCGATGATACTTTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2348

Amino Acids

274.34

Weight (kDa)

8.92

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 219 1.8e-85 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 407 - 812 6.2e-236 PROCN (NUC071) domain
RRM_4 PF10598 997 - 1087 1.4e-46 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1249 - 1384 4.1e-75 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1454 - 1612 8.7e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1772 - 2001 1.4e-129 PRP8 domain IV core
JAB PF01398 2112 - 2211 3.7e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2224 - 2344 1.3e-48 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1536, 2301, 2892
AasI GACNNNNNNGTC 1 cut(s) 5529
Acc36I ACCTGC 4 cut(s) 355, 1078, 3954, 6689
AccB1I GGYRCC 3 cut(s) 32, 1924, 2019
AccB7I CCANNNNNTGG 2 cut(s) 1913, 5447
AccBSI CCGCTC 2 cut(s) 1123, 6521
AccI GTMKAC 2 cut(s) 641, 3794
AccII CGCG 2 cut(s) 1258, 7026
AccIII TCCGGA 1 cut(s) 6856
AclI AACGTT 1 cut(s) 3800
AcoI YGGCCR 4 cut(s) 13, 3434, 4946, 6965
AcuI CTGAAG 6 cut(s) 1524, 1625, 3119, 3771, 5335, 5513
AcyI GRCGYC 2 cut(s) 2020, 4240
AdeI CACNNNGTG 1 cut(s) 4049
AfeI AGCGCT 1 cut(s) 3834
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 7 cut(s) 223, 255, 837, 3727, 4186, 5109, 6751
AgeI ACCGGT 1 cut(s) 1875
AhdI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
AjiI CACGTC 2 cut(s) 2006, 6752
AjnI CCWGG 9 cut(s) 28, 847, 1906, 2218, 4371, 4883, 5194, 6694, 6938
AjuI GAANNNNNNNTTGG 4 cut(s) 1706, 1738, 3393, 3425
AleI CACNNNNGTG 4 cut(s) 1930, 5135, 5387, 5508
AloI GAACNNNNNNTCC 6 cut(s) 3597, 3629, 4332, 4364, 4504, 4536
Alw21I GWGCWC 4 cut(s) 304, 2478, 4774, 5863
Alw26I GTCTC 6 cut(s) 237, 2381, 2633, 3125, 3266, 3518
Alw44I GTGCAC 1 cut(s) 2474
Ama87I CYCGRG 4 cut(s) 338, 3201, 3351, 5076
Aor13HI TCCGGA 1 cut(s) 6856
Aor51HI AGCGCT 1 cut(s) 3834
ApaLI GTGCAC 1 cut(s) 2474
ArsI GACNNNNNNTTYG 4 cut(s) 2665, 2697, 4225, 4257
AsiGI ACCGGT 1 cut(s) 1875
Asp700I GAANNNNTTC 2 cut(s) 1191, 6374
AspLEI GCGC 5 cut(s) 25, 177, 1260, 2022, 3835
AsuC2I CCSGG 2 cut(s) 133, 371
AvaI CYCGRG 4 cut(s) 338, 3201, 3351, 5076
AxyI CCTNAGG 4 cut(s) 2127, 5264, 6447, 6606
BaeGI GKGCMC 2 cut(s) 1927, 2478
BaeI ACNNNNGTAYC 6 cut(s) 275, 308, 857, 890, 3252, 3285
BalI TGGCCA 2 cut(s) 3436, 4948
BamHI GGATCC 2 cut(s) 1057, 5644
BanI GGYRCC 3 cut(s) 32, 1924, 2019
BanII GRGCYC 3 cut(s) 4539, 4614, 4980
BarI GAAGNNNNNNTAC 6 cut(s) 371, 403, 2311, 2343, 3603, 3635
BauI CACGAG 2 cut(s) 3661, 5506
BbsI GAAGAC 4 cut(s) 1505, 1622, 3125, 3777
Bbv12I GWGCWC 4 cut(s) 304, 2478, 4774, 5863
BbvCI CCTCAGC 2 cut(s) 102, 6594
BceAI ACGGC 2 cut(s) 28, 1659
BcgI CGANNNNNNTGC 2 cut(s) 2455, 2489
BciT130I CCWGG 9 cut(s) 30, 849, 1908, 2220, 4373, 4885, 5196, 6696, 6940
BciVI GTATCC 4 cut(s) 2926, 3253, 3612, 6779
BclI TGATCA 3 cut(s) 2800, 5956, 6529
BcnI CCSGG 2 cut(s) 133, 371
BcoDI GTCTC 6 cut(s) 237, 2381, 2633, 3125, 3266, 3518
BfaI CTAG 9 cut(s) 593, 749, 1647, 2000, 3300, 3447, 3960, 4700, 4962
BfmI CTRYAG 6 cut(s) 1485, 2859, 5310, 5559, 5702, 6682
BfoI RGCGCY 2 cut(s) 2023, 3836
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
BfuI GTATCC 4 cut(s) 2926, 3253, 3612, 6779
BglII AGATCT 3 cut(s) 1274, 4604, 5346
BlpI GCTNAGC 2 cut(s) 69, 4974
BmcAI AGTACT 2 cut(s) 5015, 6947
BmeRI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
BmeT110I CYCGRG 4 cut(s) 338, 3201, 3351, 5076
BmgBI CACGTC 2 cut(s) 2006, 6752
BmrI ACTGGG 2 cut(s) 408, 1896
BmuI ACTGGG 2 cut(s) 408, 1896
BoxI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
BpiI GAAGAC 4 cut(s) 1505, 1622, 3125, 3777
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 3 cut(s) 81, 2582, 6678
Bpu10I CCTNAGC 3 cut(s) 102, 4224, 6594
Bpu1102I GCTNAGC 2 cut(s) 69, 4974
BpuEI CTTGAG 5 cut(s) 140, 2795, 4511, 7001, 7016
BpuMI CCSGG 2 cut(s) 133, 371
Bsa29I ATCGAT 1 cut(s) 4942
BsaAI YACGTR 2 cut(s) 3128, 3728
BsaBI GATNNNNATC 4 cut(s) 585, 1062, 2793, 3474
BsaHI GRCGYC 2 cut(s) 2020, 4240
BsaI GGTCTC 3 cut(s) 237, 2633, 3125
BsaWI WCCGGW 2 cut(s) 1875, 6856
Bse118I RCCGGY 7 cut(s) 35, 61, 1508, 1875, 3218, 4314, 6963
Bse21I CCTNAGG 4 cut(s) 2127, 5264, 6447, 6606
Bse3DI GCAATG 6 cut(s) 2954, 5229, 6384, 6468, 6480, 6656
Bse8I GATNNNNATC 4 cut(s) 585, 1062, 2793, 3474
BseAI TCCGGA 1 cut(s) 6856
BseBI CCWGG 9 cut(s) 30, 849, 1908, 2220, 4373, 4885, 5196, 6696, 6940
BseCI ATCGAT 1 cut(s) 4942
BseJI GATNNNNATC 4 cut(s) 585, 1062, 2793, 3474
BseMI GCAATG 6 cut(s) 2954, 5229, 6384, 6468, 6480, 6656
BseRI GAGGAG 8 cut(s) 137, 326, 1163, 3359, 3499, 4580, 6594, 7013
BseSI GKGCMC 2 cut(s) 1927, 2478
BseX3I CGGCCG 1 cut(s) 6965
BseYI CCCAGC 3 cut(s) 1106, 1987, 2623
BsgI GTGCAG 5 cut(s) 1314, 4715, 4883, 5559, 6833
Bsh1236I CGCG 2 cut(s) 1258, 7026
Bsh1285I CGRYCG 1 cut(s) 6968
BshNI GGYRCC 3 cut(s) 32, 1924, 2019
BshTI ACCGGT 1 cut(s) 1875
BshVI ATCGAT 1 cut(s) 4942
BsiEI CGRYCG 1 cut(s) 6968
BsiHKAI GWGCWC 4 cut(s) 304, 2478, 4774, 5863
BsiHKCI CYCGRG 4 cut(s) 338, 3201, 3351, 5076
BslFI GGGAC 7 cut(s) 266, 299, 3368, 6201, 6505, 6668, 6762
BsmAI GTCTC 6 cut(s) 237, 2381, 2633, 3125, 3266, 3518
BsmBI CGTCTC 2 cut(s) 2381, 3518
BsmFI GGGAC 7 cut(s) 266, 299, 3368, 6201, 6505, 6668, 6762
BsmI GAATGC 5 cut(s) 525, 843, 971, 2702, 5170
Bso31I GGTCTC 3 cut(s) 237, 2633, 3125
BsoBI CYCGRG 4 cut(s) 338, 3201, 3351, 5076
Bsp1286I GDGCHC 8 cut(s) 304, 1927, 2478, 4539, 4614, 4774, 4980, 5863
Bsp13I TCCGGA 1 cut(s) 6856
Bsp1407I TGTACA 2 cut(s) 1041, 5026
Bsp1720I GCTNAGC 2 cut(s) 69, 4974
Bsp19I CCATGG 6 cut(s) 247, 356, 428, 1032, 4157, 6869
Bsp68I TCGCGA 1 cut(s) 7026
BspDI ATCGAT 1 cut(s) 4942
BspEI TCCGGA 1 cut(s) 6856
BspFNI CGCG 2 cut(s) 1258, 7026
BspHI TCATGA 7 cut(s) 277, 2656, 4111, 4792, 5235, 6532, 6952
BspMAI CTGCAG 4 cut(s) 2863, 5563, 5706, 6686
BspMI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
BspQI GCTCTTC 4 cut(s) 2613, 4544, 5868, 6528
BspT107I GGYRCC 3 cut(s) 32, 1924, 2019
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 3 cut(s) 237, 2633, 3125
BsrBI CCGCTC 2 cut(s) 1123, 6521
BsrDI GCAATG 6 cut(s) 2954, 5229, 6384, 6468, 6480, 6656
BsrFI RCCGGY 7 cut(s) 35, 61, 1508, 1875, 3218, 4314, 6963
BsrGI TGTACA 2 cut(s) 1041, 5026
BssAI RCCGGY 7 cut(s) 35, 61, 1508, 1875, 3218, 4314, 6963
BssNAI GTATAC 1 cut(s) 642
BssNI GRCGYC 2 cut(s) 2020, 4240
BssSI CACGAG 2 cut(s) 3661, 5506
Bst1107I GTATAC 1 cut(s) 642
Bst2BI CACGAG 2 cut(s) 3661, 5506
Bst2UI CCWGG 9 cut(s) 30, 849, 1908, 2220, 4373, 4885, 5196, 6696, 6940
BstACI GRCGYC 2 cut(s) 2020, 4240
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 4202
BstAUI TGTACA 2 cut(s) 1041, 5026
BstBAI YACGTR 2 cut(s) 3128, 3728
BstC8I GCNNGC 6 cut(s) 63, 2700, 2859, 3434, 6145, 6149
BstDSI CCRYGG 6 cut(s) 247, 356, 428, 1032, 4157, 6869
BstEII GGTNACC 2 cut(s) 942, 4650
BstFNI CGCG 2 cut(s) 1258, 7026
BstH2I RGCGCY 2 cut(s) 2023, 3836
BstHHI GCGC 5 cut(s) 25, 177, 1260, 2022, 3835
BstMAI GTCTC 6 cut(s) 237, 2381, 2633, 3125, 3266, 3518
BstMCI CGRYCG 1 cut(s) 6968
BstNI CCWGG 9 cut(s) 30, 849, 1908, 2220, 4373, 4885, 5196, 6696, 6940
BstNSI RCATGY 6 cut(s) 227, 259, 841, 1543, 4018, 5113
BstPAI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
BstPI GGTNACC 2 cut(s) 942, 4650
BstSFI CTRYAG 6 cut(s) 1485, 2859, 5310, 5559, 5702, 6682
BstSLI GKGCMC 2 cut(s) 1927, 2478
BstUI CGCG 2 cut(s) 1258, 7026
BstV2I GAAGAC 4 cut(s) 1505, 1622, 3125, 3777
BstXI CCANNNNNNTGG 4 cut(s) 357, 398, 451, 5339
BstZ17I GTATAC 1 cut(s) 642
BstZI CGGCCG 1 cut(s) 6965
Bsu15I ATCGAT 1 cut(s) 4942
Bsu36I CCTNAGG 4 cut(s) 2127, 5264, 6447, 6606
BsuI GTATCC 4 cut(s) 2926, 3253, 3612, 6779
BsuTUI ATCGAT 1 cut(s) 4942
BtgI CCRYGG 6 cut(s) 247, 356, 428, 1032, 4157, 6869
BtrI CACGTC 2 cut(s) 2006, 6752
BtsI GCAGTG 2 cut(s) 3060, 5826
BtuMI TCGCGA 1 cut(s) 7026
BveI ACCTGC 4 cut(s) 355, 1078, 3954, 6689
Cac8I GCNNGC 6 cut(s) 63, 2700, 2859, 3434, 6145, 6149
CciI TCATGA 7 cut(s) 277, 2656, 4111, 4792, 5235, 6532, 6952
CfoI GCGC 5 cut(s) 25, 177, 1260, 2022, 3835
Cfr10I RCCGGY 7 cut(s) 35, 61, 1508, 1875, 3218, 4314, 6963
ClaI ATCGAT 1 cut(s) 4942
CseI GACGC 4 cut(s) 2669, 4229, 5519, 6409
CsiI ACCWGGT 1 cut(s) 4371
CspAI ACCGGT 1 cut(s) 1875
CspCI CAANNNNNGTGG 2 cut(s) 2016, 2051
DinI GGCGCC 1 cut(s) 2021
DraIII CACNNNGTG 1 cut(s) 4049
DrdI GACNNNNNNGTC 1 cut(s) 5529
DriI GACNNNNNGTC 3 cut(s) 808, 2346, 5110
DseDI GACNNNNNNGTC 1 cut(s) 5529
EaeI YGGCCR 4 cut(s) 13, 3434, 4946, 6965
EagI CGGCCG 1 cut(s) 6965
Eam1105I GACNNNNNGTC 3 cut(s) 808, 2346, 5110
EciI GGCGGA 2 cut(s) 42, 2405
EclXI CGGCCG 1 cut(s) 6965
Eco24I GRGCYC 3 cut(s) 4539, 4614, 4980
Eco31I GGTCTC 3 cut(s) 237, 2633, 3125
Eco32I GATATC 3 cut(s) 1162, 3371, 4749
Eco47III AGCGCT 1 cut(s) 3834
Eco52I CGGCCG 1 cut(s) 6965
Eco57I CTGAAG 6 cut(s) 1524, 1625, 3119, 3771, 5335, 5513
Eco81I CCTNAGG 4 cut(s) 2127, 5264, 6447, 6606
Eco88I CYCGRG 4 cut(s) 338, 3201, 3351, 5076
Eco91I GGTNACC 2 cut(s) 942, 4650
EcoO109I RGGNCCY 2 cut(s) 3355, 6492
EcoO65I GGTNACC 2 cut(s) 942, 4650
EcoRI GAATTC 2 cut(s) 3946, 6313
EcoRII CCWGG 9 cut(s) 28, 847, 1906, 2218, 4371, 4883, 5194, 6694, 6938
EcoRV GATATC 3 cut(s) 1162, 3371, 4749
EcoT22I ATGCAT 4 cut(s) 2106, 2794, 6573, 6671
EcoT38I GRGCYC 3 cut(s) 4539, 4614, 4980
EgeI GGCGCC 1 cut(s) 2021
EheI GGCGCC 1 cut(s) 2021
Esp3I CGTCTC 2 cut(s) 2381, 3518
FaqI GGGAC 7 cut(s) 266, 299, 3368, 6201, 6505, 6668, 6762
FauI CCCGC 1 cut(s) 1263
FauNDI CATATG 2 cut(s) 2927, 4330
FbaI TGATCA 3 cut(s) 2800, 5956, 6529
FblI GTMKAC 2 cut(s) 641, 3794
FriOI GRGCYC 3 cut(s) 4539, 4614, 4980
FspBI CTAG 9 cut(s) 593, 749, 1647, 2000, 3300, 3447, 3960, 4700, 4962
GlaI GCGC 5 cut(s) 24, 176, 1259, 2021, 3834
GsaI CCCAGC 3 cut(s) 1110, 1991, 2627
GsuI CTGGAG 3 cut(s) 81, 2582, 6678
HaeII RGCGCY 2 cut(s) 2023, 3836
HgaI GACGC 4 cut(s) 2669, 4229, 5519, 6409
HhaI GCGC 5 cut(s) 25, 177, 1260, 2022, 3835
Hin1I GRCGYC 2 cut(s) 2020, 4240
Hin6I GCGC 5 cut(s) 23, 175, 1258, 2020, 3833
HinP1I GCGC 5 cut(s) 23, 175, 1258, 2020, 3833
HincII GTYRAC 4 cut(s) 1692, 1797, 3817, 4590
HindII GTYRAC 4 cut(s) 1692, 1797, 3817, 4590
HindIII AAGCTT 6 cut(s) 116, 1697, 2543, 5718, 6722, 6926
HpaI GTTAAC 1 cut(s) 3817
Hpy99I CGWCG 4 cut(s) 49, 194, 507, 1293
HpyCH4IV ACGT 9 cut(s) 2005, 2311, 2830, 3127, 3727, 3800, 4186, 4674, 6751
HpySE526I ACGT 9 cut(s) 2005, 2311, 2830, 3127, 3727, 3800, 4186, 4674, 6751
Hsp92I GRCGYC 2 cut(s) 2020, 4240
HspAI GCGC 5 cut(s) 23, 175, 1258, 2020, 3833
KasI GGCGCC 1 cut(s) 2019
KflI GGGWCCC 1 cut(s) 6492
Kpn2I TCCGGA 1 cut(s) 6856
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 3 cut(s) 2800, 5956, 6529
KspAI GTTAAC 1 cut(s) 3817
LguI GCTCTTC 4 cut(s) 2613, 4544, 5868, 6528
MabI ACCWGGT 1 cut(s) 4371
MaeI CTAG 9 cut(s) 593, 749, 1647, 2000, 3300, 3447, 3960, 4700, 4962
MaeII ACGT 9 cut(s) 2005, 2311, 2830, 3127, 3727, 3800, 4186, 4674, 6751
MbiI CCGCTC 2 cut(s) 1123, 6521
MfeI CAATTG 2 cut(s) 605, 1751
MhlI GDGCHC 8 cut(s) 304, 1927, 2478, 4539, 4614, 4774, 4980, 5863
MlsI TGGCCA 2 cut(s) 3436, 4948
MluNI TGGCCA 2 cut(s) 3436, 4948
Mly113I GGCGCC 1 cut(s) 2020
MmeI TCCRAC 9 cut(s) 594, 1262, 2463, 3156, 3391, 3870, 4760, 4818, 5622
Mox20I TGGCCA 2 cut(s) 3436, 4948
Mph1103I ATGCAT 4 cut(s) 2106, 2794, 6573, 6671
MreI CGCCGGCG 1 cut(s) 61
MroI TCCGGA 1 cut(s) 6856
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 2 cut(s) 1191, 6374
MscI TGGCCA 2 cut(s) 3436, 4948
Msp20I TGGCCA 2 cut(s) 3436, 4948
MspA1I CMGCKG 5 cut(s) 101, 760, 1229, 6151, 6465
MspCI CTTAAG 1 cut(s) 305
MunI CAATTG 2 cut(s) 605, 1751
Mva1269I GAATGC 5 cut(s) 525, 843, 971, 2702, 5170
MvaI CCWGG 9 cut(s) 30, 849, 1908, 2220, 4373, 4885, 5196, 6696, 6940
MvnI CGCG 2 cut(s) 1258, 7026
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 1 cut(s) 2020
NciI CCSGG 2 cut(s) 133, 371
NcoI CCATGG 6 cut(s) 247, 356, 428, 1032, 4157, 6869
NdeI CATATG 2 cut(s) 2927, 4330
NgoMIV GCCGGC 1 cut(s) 61
NmeAIII GCCGAG 5 cut(s) 133, 1078, 3256, 5818, 6930
NruI TCGCGA 1 cut(s) 7026
NsiI ATGCAT 4 cut(s) 2106, 2794, 6573, 6671
NspI RCATGY 6 cut(s) 227, 259, 841, 1543, 4018, 5113
OliI CACNNNNGTG 4 cut(s) 1930, 5135, 5387, 5508
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 7 cut(s) 277, 2656, 4111, 4792, 5235, 6532, 6952
PasI CCCWGGG 1 cut(s) 6939
PciI ACATGT 4 cut(s) 223, 255, 837, 5109
PciSI GCTCTTC 4 cut(s) 2613, 4544, 5868, 6528
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 1288, 2677
PctI GAATGC 5 cut(s) 525, 843, 971, 2702, 5170
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 2 cut(s) 1191, 6374
PflMI CCANNNNNTGG 2 cut(s) 1913, 5447
PfoI TCCNGGA 1 cut(s) 369
PinAI ACCGGT 1 cut(s) 1875
PluTI GGCGCC 1 cut(s) 2023
Ppu21I YACGTR 2 cut(s) 3128, 3728
PpuMI RGGWCCY 2 cut(s) 3355, 6492
PscI ACATGT 4 cut(s) 223, 255, 837, 5109
PshAI GACNNNNGTC 3 cut(s) 2829, 6158, 6864
PsiI TTATAA 3 cut(s) 1536, 2301, 2892
Psp1406I AACGTT 1 cut(s) 3800
Psp5II RGGWCCY 2 cut(s) 3355, 6492
Psp6I CCWGG 9 cut(s) 28, 847, 1906, 2218, 4371, 4883, 5194, 6694, 6938
PspEI GGTNACC 2 cut(s) 942, 4650
PspFI CCCAGC 3 cut(s) 1106, 1987, 2623
PspGI CCWGG 9 cut(s) 28, 847, 1906, 2218, 4371, 4883, 5194, 6694, 6938
PspPPI RGGWCCY 2 cut(s) 3355, 6492
PstI CTGCAG 4 cut(s) 2863, 5563, 5706, 6686
PvuII CAGCTG 4 cut(s) 101, 760, 1229, 6151
RruI TCGCGA 1 cut(s) 7026
SapI GCTCTTC 4 cut(s) 2613, 4544, 5868, 6528
ScaI AGTACT 2 cut(s) 5015, 6947
SduI GDGCHC 8 cut(s) 304, 1927, 2478, 4539, 4614, 4774, 4980, 5863
SexAI ACCWGGT 1 cut(s) 4371
SfcI CTRYAG 6 cut(s) 1485, 2859, 5310, 5559, 5702, 6682
SfoI GGCGCC 1 cut(s) 2021
Sfr274I CTCGAG 1 cut(s) 338
SgrAI CRCCGGYG 2 cut(s) 35, 61
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 7 cut(s) 119, 305, 338, 2774, 4490, 6980, 6995
SmoI CTYRAG 7 cut(s) 119, 305, 338, 2774, 4490, 6980, 6995
SspDI GGCGCC 1 cut(s) 2019
SspI AATATT 5 cut(s) 2807, 3046, 3161, 4716, 5791
SspMI CTAG 9 cut(s) 593, 749, 1647, 2000, 3300, 3447, 3960, 4700, 4962
TaiI ACGT 9 cut(s) 2008, 2314, 2833, 3130, 3730, 3803, 4189, 4677, 6754
TaqII GACCGA 1 cut(s) 930
TatI WGTACW 7 cut(s) 960, 1041, 1818, 3154, 5013, 5026, 6945
TauI GCSGC 4 cut(s) 28, 68, 1126, 3028
TspGWI ACGGA 9 cut(s) 1715, 2418, 2499, 3848, 4046, 4757, 6047, 6281, 6798
Van91I CCANNNNNTGG 2 cut(s) 1913, 5447
Vha464I CTTAAG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 2474
XbaI TCTAGA 2 cut(s) 592, 3299
XceI RCATGY 6 cut(s) 227, 259, 841, 1543, 4018, 5113
XcmI CCANNNNNNNNNTGG 2 cut(s) 4415, 5934
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 2 cut(s) 641, 3794
XmnI GAANNNNTTC 2 cut(s) 1191, 6374
XspI CTAG 9 cut(s) 593, 749, 1647, 2000, 3300, 3447, 3960, 4700, 4962
ZrmI AGTACT 2 cut(s) 5015, 6947
Zsp2I ATGCAT 4 cut(s) 2106, 2794, 6573, 6671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.