MD09G1127300.v1.1

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000798
Physical Location & Seq
Forward (+)
9809280 .. 9809690
411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1127300.v1.1.491

Sequence Viewer

Length: 411 bp
ATGATGCAAATTGCTGGTTACTTGCACGGTGTAAGCCCACCCGACAACCCTCGGGTTAAGGAAATTCGACGCATGGTCATGCCACCACAGTGGGGGACTCACCAGCAAGTTCATCTGCCATCAGCTCTTTCTGAGAATGATTTCCTCAATGATTTAGAGCCTTTGGGATGGATGCACACGCAGCCAAATGAGCTTCCTCAGCTTTCTTTCCAGGATGTCGCATGGCTGGAGAACACCAAGCAAGGGAATGGGGAGAAGTGCAACATTTTAACCTGCAGCTTCACTCCAGGTTCTTGCTGGTTGCCTGCATGTAAGCTTACTCTGGCGGGTTATGAGTGGGGACGTAGTGCTAATACAGGAGACAGCGGAAGCAAAGTTAATCCACAAGGATACCTTTCCAACACATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

137

Amino Acids

15.16

Weight (kDa)

5.72

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 3 - 75 4.9e-08 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 90 - 132 2.2e-06 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
AciI CCGC 2 cut(s) 326, 366
AcsI RAATTY 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 92, 243
AhdI GACNNNNNGTC 1 cut(s) 74
AjnI CCWGG 2 cut(s) 210, 286
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 5 cut(s) 125, 193, 202, 279, 316
AluI AGCT 5 cut(s) 125, 193, 202, 279, 316
Alw26I GTCTC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 181, 276
ApoI RAATTY 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 193, 288
BccI CCATC 2 cut(s) 127, 162
BciT130I CCWGG 2 cut(s) 212, 288
BciVI GTATCC 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 281
BfuI GTATCC 1 cut(s) 383
BisI GCNGC 2 cut(s) 182, 277
BlsI GCNGC 2 cut(s) 183, 278
Bme1390I CCNGG 2 cut(s) 212, 288
BmeRI GACNNNNNGTC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 51
BmrFI CCNGG 2 cut(s) 212, 288
BmsI GCATC 1 cut(s) 162
BpmI CTGGAG 2 cut(s) 248, 270
Bpu10I CCTNAGC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 50
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 243
BseBI CCWGG 2 cut(s) 212, 288
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 3 cut(s) 173, 177, 220
BseLI CCNNNNNNNGG 2 cut(s) 92, 243
BseMII CTCAG 2 cut(s) 123, 212
BseXI GCAGC 2 cut(s) 193, 288
BsiHKCI CYCGRG 1 cut(s) 51
BslFI GGGAC 2 cut(s) 109, 354
BslI CCNNNNNNNGG 2 cut(s) 92, 243
BsmAI GTCTC 1 cut(s) 354
BsmFI GGGAC 2 cut(s) 109, 354
BsoBI CYCGRG 1 cut(s) 51
BspACI CCGC 2 cut(s) 326, 366
BspCNI CTCAG 2 cut(s) 124, 211
BspMAI CTGCAG 1 cut(s) 278
BspMI ACCTGC 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 212, 288
Bst4CI ACNGT 2 cut(s) 29, 90
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 2 cut(s) 132, 198
BstF5I GGATG 3 cut(s) 173, 177, 220
BstMAI GTCTC 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 181, 190, 199
BstNI CCWGG 2 cut(s) 212, 288
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 2 cut(s) 210, 286
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 193, 288
BstXI CCANNNNNNTGG 1 cut(s) 90
BsuI GTATCC 1 cut(s) 383
BtsCI GGATG 3 cut(s) 173, 177, 220
BtsIMutI CAGTG 1 cut(s) 95
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 306
CseI GACGC 1 cut(s) 78
CviAII CATG 4 cut(s) 73, 79, 222, 309
CviJI RGCY 9 cut(s) 36, 125, 160, 184, 193, 202, 226, 279, 316
CviKI_1 RGCY 9 cut(s) 36, 125, 160, 184, 193, 202, 226, 279, 316
DdeI CTNAG 2 cut(s) 132, 198
DriI GACNNNNNGTC 1 cut(s) 74
Eam1105I GACNNNNNGTC 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 210, 286
FaeI CATG 4 cut(s) 76, 82, 225, 312
FaiI YATR 5 cut(s) 74, 80, 223, 310, 333
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 109, 354
FatI CATG 4 cut(s) 72, 78, 221, 308
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 182, 277
FokI GGATG 3 cut(s) 180, 184, 227
Fsp4HI GCNGC 2 cut(s) 182, 277
GluI GCNGC 2 cut(s) 182, 277
GsuI CTGGAG 2 cut(s) 248, 270
HgaI GACGC 1 cut(s) 78
Hin1II CATG 4 cut(s) 76, 82, 225, 312
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 1 cut(s) 97
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 133, 410
Hpy99I CGWCG 1 cut(s) 72
HpyCH4III ACNGT 2 cut(s) 29, 90
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 6 cut(s) 7, 25, 175, 261, 276, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 190, 199
HpyF3I CTNAG 2 cut(s) 132, 198
HpySE526I ACGT 1 cut(s) 343
Hsp92II CATG 4 cut(s) 76, 82, 225, 312
Lsp1109I GCAGC 2 cut(s) 193, 288
LweI GCATC 1 cut(s) 162
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 1 cut(s) 17
MluCI AATT 2 cut(s) 9, 63
MlyI GAGTC 1 cut(s) 91
MnlI CCTC 3 cut(s) 60, 155, 207
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 3 cut(s) 57, 269, 378
MslI CAYNNNNRTG 2 cut(s) 77, 88
MspA1I CMGCKG 1 cut(s) 366
MspR9I CCNGG 2 cut(s) 212, 288
MvaI CCWGG 2 cut(s) 212, 288
MwoI GCNNNNNNNGC 3 cut(s) 181, 190, 199
NlaIII CATG 4 cut(s) 76, 82, 225, 312
NspI RCATGY 1 cut(s) 312
OliI CACNNNNGTG 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 2 cut(s) 183, 278
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Psp6I CCWGG 2 cut(s) 210, 286
PspGI CCWGG 2 cut(s) 210, 286
PstI CTGCAG 1 cut(s) 278
RseI CAYNNNNRTG 2 cut(s) 77, 88
SaqAI TTAA 3 cut(s) 57, 269, 378
SatI GCNGC 2 cut(s) 182, 277
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 212, 288
SetI ASST 9 cut(s) 127, 195, 204, 275, 281, 292, 318, 346, 396
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 77, 88
Sse9I AATT 2 cut(s) 9, 63
SsiI CCGC 2 cut(s) 326, 366
StyD4I CCNGG 2 cut(s) 210, 286
TaaI ACNGT 2 cut(s) 29, 90
TaiI ACGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 67
TasI AATT 2 cut(s) 9, 63
Tru1I TTAA 3 cut(s) 57, 269, 378
Tru9I TTAA 3 cut(s) 57, 269, 378
TscAI CASTG 1 cut(s) 95
TseI GCWGC 2 cut(s) 181, 276
TspDTI ATGAA 1 cut(s) 101
TspRI CASTG 1 cut(s) 95
XapI RAATTY 1 cut(s) 63
XceI RCATGY 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.