MD09G1102300.v1.1

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000798
Physical Location & Seq
Reverse (-)
7481672 .. 7482565
894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1102300.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGTTATGTACATTAAAACTGAGGATCCTGGTCTGCCAGCATTTTACTATGATCCGTTGGTGCATCCAATACCCAGCACTAGCACGAAGAGAATTAATGATGTCGATGACAGTGAGGATCATGGTTTTGTTTTACCGAAAGTGGTGGAACCGTTTCTGAAAGATACTCCGCTTCATAGTGACACCACGACTGCTGGAAAGCTGTGGAACTTGAACAACTATAGAACCGACGTTATCCAAGCTCTTGGAGGTGTTGAGGCAATACTTGAGCACACATTGTTCAAAGGAACATACTTCCCGACTTGGGAGGGTCTTTTCTGGGAGAAGGCATCTGGCTTTGAGGAATCTATGAAGTATAAGAAGTTAACTAATGCACAAGGATCTGGGTTCAACCAAATTCCCAATTGTAGGTTTACCCTTTGGTGGTCGCCTACCATCAATCTGGCCAATGTATATGTGGGTTTCCAAGGGCAGTTGGATCTAACAGGAATCTTTACGCATGGGAAGATACCGACCTTGAAGATATTTTTGATTCAGATATTTCGTGCACACTTGTGGCAGAAGATTCATGAAAGTGTTGTCATGGATCTCTGTCAAGTCCTAGACCAGGAGCTGGATGCTTTCGAAATCGAAACGGTGCAGAAAGAAACAATACATCCAAGGAAGAGTTACAAAATGAACAGCTCATGTGCCGACATTCTTTTCTTTGCAGCCCATAAATGGCCAATGGCGAAACCAAGTCTTGTGGCTGAGCCAAACGATGTGTTCAATCAGAAAGCAAGCAATAAGTACTGGATAGATGTACAACTCCGTTGGGGAGACTACGATTCACCAGAGCCAAGCTTATGGATTTGTACAACTGACAACATGTCCCTCTATCCTTCTCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

298

Amino Acids

34.06

Weight (kDa)

5.46

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U6-snRNA_bdg PF10596 60 - 204 4.3e-74 U6-snRNA interacting domain of PrP8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 613
AciI CCGC 1 cut(s) 170
AclWI GGATC 7 cut(s) 20, 33, 47, 126, 388, 486, 594
AcoI YGGCCR 2 cut(s) 444, 722
AcsI RAATTY 1 cut(s) 396
AfaI GTAC 4 cut(s) 11, 791, 804, 856
AfiI CCNNNNNNNGG 6 cut(s) 304, 408, 423, 607, 613, 720
AflIII ACRYGT 1 cut(s) 867
AgsI TTSAA 5 cut(s) 214, 283, 391, 520, 769
AhdI GACNNNNNGTC 1 cut(s) 868
AjnI CCWGG 2 cut(s) 28, 606
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 5 cut(s) 202, 242, 613, 684, 843
AluI AGCT 5 cut(s) 202, 242, 613, 684, 843
Alw21I GWGCWC 2 cut(s) 273, 550
Alw26I GTCTC 1 cut(s) 813
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 7 cut(s) 20, 33, 47, 126, 388, 486, 594
AlwNI CAGNNNCTG 1 cut(s) 613
AoxI GGCC 2 cut(s) 444, 722
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 1 cut(s) 710
ApoI RAATTY 1 cut(s) 396
AseI ATTAAT 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 822
AsuII TTCGAA 1 cut(s) 624
BaeGI GKGCMC 1 cut(s) 550
BalI TGGCCA 2 cut(s) 446, 724
BamHI GGATCC 1 cut(s) 25
Bbv12I GWGCWC 2 cut(s) 273, 550
BbvI GCAGC 1 cut(s) 722
BccI CCATC 1 cut(s) 443
BciT130I CCWGG 2 cut(s) 30, 608
BcoDI GTCTC 1 cut(s) 813
BfaI CTAG 2 cut(s) 81, 602
BfmI CTRYAG 1 cut(s) 220
BisI GCNGC 1 cut(s) 711
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 712
BmcAI AGTACT 1 cut(s) 791
Bme1390I CCNGG 2 cut(s) 30, 608
BmeRI GACNNNNNGTC 1 cut(s) 868
BmiI GGNNCC 2 cut(s) 27, 150
BmrFI CCNGG 2 cut(s) 30, 608
BmsI GCATC 3 cut(s) 73, 338, 607
Bpu1102I GCTNAGC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 466, 659
Bsc4I CCNNNNNNNGG 6 cut(s) 304, 408, 423, 607, 613, 720
Bse1I ACTGG 1 cut(s) 797
BseBI CCWGG 2 cut(s) 30, 608
BseDI CCNNGG 2 cut(s) 466, 659
BseGI GGATG 3 cut(s) 64, 622, 655
BseLI CCNNNNNNNGG 6 cut(s) 304, 408, 423, 607, 613, 720
BseMII CTCAG 2 cut(s) 12, 741
BseNI ACTGG 1 cut(s) 797
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 1 cut(s) 722
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 659
BshFI GGCC 2 cut(s) 446, 724
BsiHKAI GWGCWC 2 cut(s) 273, 550
BslFI GGGAC 1 cut(s) 856
BslI CCNNNNNNNGG 6 cut(s) 304, 408, 423, 607, 613, 720
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 856
BsnI GGCC 2 cut(s) 446, 724
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 273, 550
Bsp1407I TGTACA 3 cut(s) 9, 802, 854
Bsp143I GATC 6 cut(s) 25, 52, 118, 380, 478, 586
Bsp1720I GCTNAGC 1 cut(s) 750
BspACI CCGC 1 cut(s) 170
BspANI GGCC 2 cut(s) 446, 724
BspCNI CTCAG 2 cut(s) 13, 742
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 27, 150
BspPI GGATC 7 cut(s) 20, 33, 47, 126, 388, 486, 594
BspT104I TTCGAA 1 cut(s) 624
BsrGI TGTACA 3 cut(s) 9, 802, 854
BsrI ACTGG 1 cut(s) 797
BssECI CCNNGG 2 cut(s) 466, 659
BssMI GATC 6 cut(s) 25, 52, 118, 380, 478, 586
BssT1I CCWWGG 2 cut(s) 466, 659
Bst2UI CCWGG 2 cut(s) 30, 608
Bst4CI ACNGT 3 cut(s) 113, 153, 637
Bst6I CTCTTC 2 cut(s) 83, 659
BstAUI TGTACA 3 cut(s) 9, 802, 854
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 2 cut(s) 39, 781
BstDEI CTNAG 2 cut(s) 21, 750
BstENI CCTNNNNNAGG 1 cut(s) 605
BstF5I GGATG 3 cut(s) 64, 622, 655
BstKTI GATC 6 cut(s) 28, 55, 121, 383, 481, 589
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 6 cut(s) 25, 52, 118, 380, 478, 586
BstNI CCWGG 2 cut(s) 30, 608
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 2 cut(s) 28, 606
BstSFI CTRYAG 1 cut(s) 220
BstSLI GKGCMC 1 cut(s) 550
BstV1I GCAGC 1 cut(s) 722
BstX2I RGATCY 4 cut(s) 25, 380, 478, 586
BstXI CCANNNNNNTGG 3 cut(s) 245, 442, 846
BstYI RGATCY 4 cut(s) 25, 380, 478, 586
BsuRI GGCC 2 cut(s) 446, 724
BtsCI GGATG 3 cut(s) 64, 622, 655
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 39, 781
CaiI CAGNNNCTG 1 cut(s) 613
CciI TCATGA 1 cut(s) 568
Csp6I GTAC 4 cut(s) 10, 790, 803, 855
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 7 cut(s) 122, 500, 569, 583, 687, 868, 891
CviQI GTAC 4 cut(s) 10, 790, 803, 855
DdeI CTNAG 2 cut(s) 21, 750
DpnI GATC 6 cut(s) 27, 54, 120, 382, 480, 588
DpnII GATC 6 cut(s) 25, 52, 118, 380, 478, 586
DriI GACNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 2 cut(s) 444, 722
Eam1104I CTCTTC 2 cut(s) 83, 659
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 2 cut(s) 83, 659
Eco130I CCWWGG 2 cut(s) 466, 659
EcoNI CCTNNNNNAGG 1 cut(s) 605
EcoRII CCWGG 2 cut(s) 28, 606
EcoT14I CCWWGG 2 cut(s) 466, 659
ErhI CCWWGG 2 cut(s) 466, 659
FaeI CATG 7 cut(s) 125, 503, 572, 586, 690, 871, 894
FaqI GGGAC 1 cut(s) 856
FatI CATG 7 cut(s) 121, 499, 568, 582, 686, 867, 890
Fnu4HI GCNGC 1 cut(s) 711
FokI GGATG 3 cut(s) 51, 629, 642
Fsp4HI GCNGC 1 cut(s) 711
FspBI CTAG 2 cut(s) 81, 602
GluI GCNGC 1 cut(s) 711
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 2 cut(s) 446, 724
Hin1II CATG 7 cut(s) 125, 503, 572, 586, 690, 871, 894
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HindIII AAGCTT 1 cut(s) 841
HinfI GANTC 5 cut(s) 344, 489, 532, 565, 827
HpaI GTTAAC 1 cut(s) 366
HphI GGTGA 1 cut(s) 822
Hpy166II GTNNAC 3 cut(s) 366, 414, 548
Hpy188I TCNGA 3 cut(s) 159, 537, 774
Hpy188III TCNNGA 2 cut(s) 298, 569
Hpy8I GTNNAC 3 cut(s) 366, 414, 548
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 319, 891
HpyCH4III ACNGT 3 cut(s) 113, 153, 637
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 64, 374, 548, 640, 710
HpyF3I CTNAG 2 cut(s) 21, 750
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 7 cut(s) 125, 503, 572, 586, 690, 871, 894
KspAI GTTAAC 1 cut(s) 366
Kzo9I GATC 6 cut(s) 25, 52, 118, 380, 478, 586
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 1 cut(s) 722
LweI GCATC 3 cut(s) 73, 338, 607
MaeI CTAG 2 cut(s) 81, 602
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 179, 668
MalI GATC 6 cut(s) 27, 54, 120, 382, 480, 588
MboI GATC 6 cut(s) 25, 52, 118, 380, 478, 586
MboII GAAGA 5 cut(s) 100, 517, 532, 574, 676
MfeI CAATTG 1 cut(s) 403
MflI RGATCY 4 cut(s) 25, 380, 478, 586
MhlI GDGCHC 2 cut(s) 273, 550
MlsI TGGCCA 2 cut(s) 446, 724
MluCI AATT 3 cut(s) 93, 396, 403
MluNI TGGCCA 2 cut(s) 446, 724
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 7 cut(s) 16, 109, 242, 250, 301, 334, 884
Mox20I TGGCCA 2 cut(s) 446, 724
MroXI GAANNNNTTC 1 cut(s) 153
MscI TGGCCA 2 cut(s) 446, 724
MseI TTAA 3 cut(s) 15, 96, 365
MslI CAYNNNNRTG 2 cut(s) 553, 573
Msp20I TGGCCA 2 cut(s) 446, 724
MspR9I CCNGG 2 cut(s) 30, 608
MunI CAATTG 1 cut(s) 403
MvaI CCWGG 2 cut(s) 30, 608
NdeII GATC 6 cut(s) 25, 52, 118, 380, 478, 586
NlaIII CATG 7 cut(s) 125, 503, 572, 586, 690, 871, 894
NlaIV GGNNCC 2 cut(s) 27, 150
NmuCI GTSAC 1 cut(s) 179
NspI RCATGY 1 cut(s) 871
NspV TTCGAA 1 cut(s) 624
OliI CACNNNNGTG 1 cut(s) 553
PagI TCATGA 1 cut(s) 568
PciI ACATGT 1 cut(s) 867
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 5 cut(s) 344, 489, 532, 565, 827
PflMI CCANNNNNTGG 1 cut(s) 613
PkrI GCNGC 1 cut(s) 712
PscI ACATGT 1 cut(s) 867
PshBI ATTAAT 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 28, 606
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 28, 606
PspN4I GGNNCC 2 cut(s) 27, 150
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 4 cut(s) 25, 380, 478, 586
RsaI GTAC 4 cut(s) 11, 791, 804, 856
RsaNI GTAC 4 cut(s) 10, 790, 803, 855
RseI CAYNNNNRTG 2 cut(s) 553, 573
SaqAI TTAA 3 cut(s) 15, 96, 365
SatI GCNGC 1 cut(s) 711
Sau3AI GATC 6 cut(s) 25, 52, 118, 380, 478, 586
ScaI AGTACT 1 cut(s) 791
ScrFI CCNGG 2 cut(s) 30, 608
SduI GDGCHC 2 cut(s) 273, 550
SetI ASST 9 cut(s) 204, 234, 244, 253, 413, 518, 615, 686, 845
SfaNI GCATC 3 cut(s) 73, 338, 607
SfcI CTRYAG 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 553, 573
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 93, 396, 403
SsiI CCGC 1 cut(s) 170
SspMI CTAG 2 cut(s) 81, 602
StyD4I CCNGG 2 cut(s) 28, 606
StyI CCWWGG 2 cut(s) 466, 659
TaaI ACNGT 3 cut(s) 113, 153, 637
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 105, 624, 630
TasI AATT 3 cut(s) 93, 396, 403
TatI WGTACW 4 cut(s) 9, 789, 802, 854
TfiI GAWTC 5 cut(s) 344, 489, 532, 565, 827
Tru1I TTAA 3 cut(s) 15, 96, 365
Tru9I TTAA 3 cut(s) 15, 96, 365
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 5 cut(s) 164, 365, 557, 585, 692
TspGWI ACGGA 2 cut(s) 45, 800
TspRI CASTG 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 613
VneI GTGCAC 1 cut(s) 546
VspI ATTAAT 1 cut(s) 96
XagI CCTNNNNNAGG 1 cut(s) 605
XapI RAATTY 1 cut(s) 396
XceI RCATGY 1 cut(s) 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 2 cut(s) 81, 602
ZrmI AGTACT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.