Rh2BG563900

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000798
Physical Location & Seq
Forward (+)
77524688 .. 77532001
7314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG563900.1

Sequence Viewer

Length: 6150 bp
ATGGAGCAAGGGGAGGAGGATTGGAATGAGTTTAATGACATAAACAAGCTCATTATTCGGTCTCCGCTGAGATCAGAGTACAAAATTGCATTCCCTAATCTTTACAACAACAGGCCAAGGAAGGTAAAGCTCTCTGTTTATCACACACCACTGGTTATGCATATTAAAACTGAGGATCCTGACCTGCCTGCATTTTATTATGATCCATTGCTAAACCCAATACCGGGCACCAACAGGAGTAGTTATGATAATAGTGGGGAAGATGGTGATTTTGTTTTGCCTGAAGGGGTGGAGCCTCTTTTGAGTCATACTCAGCTTTATACTGACACCACTGCTGCTGGGATTTCCCTGTATTTTGCCCCACGCCCTTTTAACTTGAGGTCTGGTCGGACTCGTCGTGCAGAGGATATACCTCTTGTGTCAGAGTGGTTTAAGGAGCATTGTCCTCGGTCATATCCTGTTAAGGTTCGAGTCAGCTATCAGAAGTTGTTGAAATCGTTTGTGTTGAATGAGCTGCATCGTAGGCCTCCCAAGGCACAAAAGAAGAAGAGCTTGTTTCGGTCTCTTCAGGCTACCAAGTTTTTCCAAACTACTGAGCTTGATTGGGTTGAAGCGGGTCTTCAACTCTGTAGGCAGGGTCATAACATGCTAAATCTGCTGATACATAGGAAAGGTCTGAACTATCTTCACCTTGATTATAATTTTAATCTCAAGCCTGTGAAAACCCTGACAACAAAAGAGCGTAAGAAATCTCGGTTTGGTAATCCTTTTCATCTGTTGCGTGAAATCTTGCGGTTAACAAAGCTTGTAGTTGATGCCCAGGTCCAGTTCCGTTTGGGTAATATTGATGCCTTTCAATTGGCTGATGGTCTGCAGTACACATTTTCTCATGTTGGTCAGTTAACTGGAATGTACCGTTACAAGTATAGGTTGATGCGGCAGATTAGAATGAGCAAAGATTTGAAGCACTTGATTTACTACCGTTTCAATACTGGGCCGGTCGGGAAAGGGCCGGGATGTGGATTCTGGGCACCAATGTGGAGGATGTGGTTGTCCTTCCTTCGTGGCATGGTCCCTCTTCTGGAACAGTGGTTGGGAAATTTACTTGCTAGACAATTTGAAGGACGCCATTCTGTGGCAAAGACAGTAACTAAGCAGCGTGTTGAAAGCAATTTCGACTTGGAGTTTCGAGCAGATGTCTTGAAAGATATTCTGGATGCCATGCCAGTGGGCATGAAGCAAAATAAGGCGAAAACAATTATGCAGCATCTCAGTGAGGCATGGCGTTGTTGGAAAGCAAATATTCCTTGGAAGGTTCCTGGTATGCCTGCTCCGGTTGAGAATATAATTCTTCGTTATGTCAAGTCAAAGGCAGATTGGTGGACACATGGTGCTCATTATAACCGTGAACGAATTAGAAGAGGTGCTACAGTGGATAAGACAGTTTGTCGAAAGAATCTTGGAAGATTAACTCGCCTTTGGCTAAAGGGAGAGCAGGAGCGACAGCACAATTATCTCAAAGATGGTCCATATATTTCACCAGAAGAAGCAGTAGCAATTTACTCAACAACAGTGCATTGGTTGGAGTCAAGAAATTTCCAACATATTCCATTTCCCCCGTTGTTGTATAAGCATGATACCAAGCTACTTATCCTTGCTCTGGAGAGGTTGAAGGAATCTTATACTGCGGCGGTAAAATTGAACCAGCATCAAAGAGAGGAGTTGGGTCTCATTGAACAAGCCTATGATAACCCCCATGAGGCATTGATACGGATAAAACGTCACTTGATCTCACAGCGTGCCTTCAAAGACGTGAGCATAGAGTTCATGGATCTGTACAGCCATCATATTCCAGTTTTTGAGATTGACCCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGCTGACAAACGTCAGCTCTTCCCGAACTGGATCAAGCCTGCAGACTCAGAGCCACCTCCACTTTTGGTCTATAAATGGTGTCAGGGGATAAACAATTTAGAGAGTATATGGGACACAAGTGAGGGGGAGTGTGTTGTGATGCTTCAGACAAAACTTGAGAGGTTATCTGAGAAGATTGACTTGACATTGCTAAATAGGCTTCTACGTCTTGTTCTTGACCATAACATTGCTGATTATATCACTGCAAAAAACAATGTGGTATTGTCATACAAAGATATGAGTCATACAAATTCATATGGTCTTATACGGGGTCTCCAGCCTGTATCATTTGTTGTACAGTATTATGGGCTTGTTTTGGATCTAATGCTTCTTGGATTGACTCGGGCCAATGAAATTGCAGGTCCACAGCAGAAGCCAAATGAGTTCATGACTTATGGGGCCACAAAGGTTGAGACTTGCCATCCTATCAGGCTGTACGCTCGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACTCAGGAGGAGGCTCGGGACCTCATACAGCGATATCTTACCGAGCATCCAGATCCCAATAATGAAAATATGGTTGGATATAATAATAAGAAGTGCTGGGCAAGAGATGCAAGGATGAGGCTCCTGAAACATGATGTCAATCTTGGAAGGAGTGTCTTTTGGAATGTCAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAATGGGAGGACAGTTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTTTTCAGCATGTGTGGATTTGAAGTAAGAATACTTCCCAAGATAATAATGAGTACGAAGGAACACCAAAGAGATGGAGTTTGGAACTTGCAAAATGAACAGACAAAGGAAAGGACTGCTGTTGCTTTTTTACGTGTTGAGGATGAATACATGAAGGCGTTTGAGAATCGTGTAAGGCAGATCCTTATGTCCTCAGGGTCTACAACATTCACAAAAGTTATCAACAAATGGAATACTGCCCTTATTGGTCTTATGACTTACTTCCGTGAAGCAGTTGTACATACAGCGGAGCTGTTAGATTTACTGGTCAAATGTGAAAATAAAATTCAGACCCGCATTAAGATTGGATTGAATTCAAAGATGCCAAGCAGGTTCCCACCTGTCATTTTTTACACGCCAAAAGAAATTGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAGAGTGACCTCAGATACTCTCGGCAGACAGAAATTGGTGTGACACACTTTAGGAGTGGAATGAGTCATGAAGAAGAGCAGATGATTCCAAATCTTTACCGTTACATACAACCATGGGAGAGTGAGTTTATAGATTCACAGCGTGTTTGGGCAGAATATGCACTAAAAAGACAGGAAGCTATGGAACAGAATCGGCGTCTTACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACATTGTTCCAGAAGGATCGACACACTTTAGCATATGACAAAGGGTGGAGAGTTCGCACAGATTTTAAGCAGTACCAGATCTTGAAGCAAAATCCTTTCTGGTGGACACACCAAAGGCATGATGGAAAATTGTGGAATTTGAATAATTATAGAACTGATGTGATCCAAGCTCTTGGAGGAGTTGATGGAATACTTGAGCACACCTTATTCAAAGGAACATACTTCCCTACTTGGGAGGGTCTCTTTTGGGAGAAGGCATCTGGATTTGAGGAATCGATGAAGTATAAAAAGTTGACTAATGCCCAAAGATCTGGCCTCAACCAAATCCCCAATCGTAGGTTTACACTTTGGTGGTCGCCTACCATAAATCGAGCCAATGTATATGTCGGTTTTCAAGTACAGCTGGATCTGACAGGAATCTTTATGCATGGGAAGATCCCAACCTTGAAGATATCGTTGATTCAGATATTCCGTGCACACTTGTGGCAGAAGATTCATGAAAGTGTTGTGATGGACCTTTGTCAGGTTTTGGATCAGGAGTTGGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACCATACATCCCAGGAAGAGTTACAAACTGAACAGCTCTTGTGCAGACATTCTCCTCTTTTCTGCTCATAAATGGCAAATGTCGAAACCTAGTCTTGTGGCTGAGCCGAAGGATGAGTATGATAAAAAAACAAACAACAAGTACTGGATAGACGTGCAGCTCCGTTGGGGGGATTATGATTCTCATGATATTGAACGTTACACCCGGGCAAAGTTTATGGACTATACAACTGATAACATGTCTATCTATCCTTCTCCGATGGGAGTGATGATTGGTATTGATTTAGCCTATAATGTGCATTCTGCATTTGGTAACTGGTTTCCCGGATCAAAACCACTGATTCAACAGGCAATGAACAAGATCATGAAGTCAAATCCGGCTTTATATGTGTTGAGGGAGCGCATAAGGAAAGGATTGCAGTTGTATTCCTCGGAGCCTACAGAGCCATATTTGTCTTCCCAAAACTATGGGGAGCTCTTCAGCAATCAAATTAAATGGTTTGTTGACGATACAAATGTGTATCGTGTTACAATGCACAAGACTTTTGACGGAAATCTTGTTACAAAACCCATCAATGGTGCTATCTTCATGTTCAATCCAGGGACTGGGCAGCTGTTCTTGAAGGTTATCCACACAAGTGTGTGGGCAGGACAGAAGCGACTTGGGCAGTTGGCTAAATGGAAAACTGCGGAAGAAGTTGCTGCTCTTGTGCGTTCTCTACCTGTTGAAGAACAGCCAAAGCAAATTATTGTGACTCGTAAGGGAATGCTCGACCCATTGGAGGTTCATTTACTGGATTTTCCCAACATAGTCATAAAAGGAAGTGAGCTACAGCTTCCTTTTCAGGTTTGCTTGAAAATTGAGAAGTTGAATGATCTGGTACTAAAGGCTACTGAGCCACAGATGGTTCTTTTTAATGTTTACGATGATTGGTTGAAGAGTGTTTCATCATACACTGCATTCTCTCGGCTCATTCTGATTCTTCGGGCACTGCATGTGAACAGAGACAAGGCAAGTATGTTGTTGAAGCCTGACCGAACAGTCATTAGTGAGCCACACCACATTTGGCCTTCTCTTTCTGATGAGCAATGGATGAAGGTTGAGCGTGCTCTAACAGATCTCATTCTGTCAGACTATGCGAAAAAGAATAATGTGAATACTTCAGCCCTCACACAGTCTGAAATTCGTGATATAATACTTGGAGCAGAGATTACTCCACCTTCTCAACAGAGGCAAGAGATAGCAGACATTGAGAAACAGGCTAAAGGAGTTAGTCAGCTGACAGCAGTCCCCATGAGGACCACAAACGTGCATGGAGATGAGCTTATTTCCACCACTACGAGTCAATATGAGCAAGCTGCATTCAGGTCCAAAACTGATTGGCGTGTGAGAGCAATATCAGCTACAAATCTACATCTTCGTGTCAACCATATTTATGTGAGTTCAGAAGATACAAAGGAAACAGGCTACACTTACATCATGCCAATGAACATTTTGAAAAAATTTATCTGTGTTGCTGATCTGAGGACACAAGTTGCTGGTTACTTGTATGGTATAAGCCCACCTGACAACCCTCAGATTAAGGAAATTCGCTGCATTGTGATGCCACCACAGCGGGGCACCCACCAGCAAGTTCATCTTCCATCAGCTCTTCCTGAGCATGATTTCCTCAATTATTTGGAACCTCTGGGATGGATGCACACCCAGCCAAATGAGCTACCTCAGCTTTCTCCTCAGGATCTCACCACTCATGCTAACATGTTGGAGAATGGCAAGCAATGGGATAGGGAGAAATGCATTATTATGACCTGCGCTTTTACTCCAGGTTCCTGCTCGTTAACTGCATATAAACTTACTCCTTCTGGTTATGAGTGGGGATGTTGTTCTAACAAGAACAGCGGAAGAAACAATAATCCACTAGATGGATACCTTCCAACTCATTATGAGAAGGTCCAGATGCTTCTCAGTGATCGCTTCCTTGGTGTCTACATGGTTCCTGATAATTGTCCATGGAACTTCAATTTCATGGGAGTGAGGCATGCATGTGACATGAAGTATGGCATCAAGCTTGGACCACCCAAGGAGTACTATCACGAGGAACACCGACCAACCCATTTCATGGAGTTCAGCGAACTAGATAAGGATGACCCGATGGAGTTAGATCGAGATTATGCCTACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2049

Amino Acids

239.02

Weight (kDa)

8.97

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROCN PF08083 109 - 512 1.4e-225 PROCN (NUC071) domain
RRM_4 PF10598 697 - 786 2.7e-44 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 947 - 1082 6.4e-75 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1152 - 1310 1.6e-88 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1470 - 1699 9.2e-126 PRP8 domain IV core
JAB PF01398 1812 - 1908 1.9e-10 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 1922 - 2044 1.4e-42 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 699, 1401
AasI GACNNNNNNGTC 1 cut(s) 5006
Acc36I ACCTGC 4 cut(s) 192, 2309, 3048, 5783
AccB1I GGYRCC 3 cut(s) 227, 1030, 5585
AccB7I CCANNNNNTGG 4 cut(s) 1135, 4571, 5888, 6085
AccI GTMKAC 2 cut(s) 2888, 5952
AclI AACGTT 1 cut(s) 4162
AcuI CTGAAG 5 cut(s) 303, 551, 2048, 4429, 5112
AcyI GRCGYC 2 cut(s) 1126, 3334
AdeI CACNNNGTG 4 cut(s) 1391, 1799, 3143, 3281
AflIII ACRYGT 3 cut(s) 2821, 4203, 5724
AhdI GACNNNNNGTC 1 cut(s) 1446
AjiI CACGTC 2 cut(s) 1813, 4120
AjnI CCWGG 5 cut(s) 819, 1318, 3977, 4564, 5788
AjuI GAANNNNNNNTTGG 2 cut(s) 2493, 2525
AleI CACNNNNGTG 6 cut(s) 1036, 3739, 3871, 4602, 4604, 5273
Alw21I GWGCWC 5 cut(s) 1396, 3591, 3868, 4443, 5077
Alw26I GTCTC 9 cut(s) 66, 567, 1733, 2237, 2366, 2618, 3635, 3944, 4965
Alw44I GTGCAC 1 cut(s) 3864
AlwNI CAGNNNCTG 1 cut(s) 4571
Ama87I CYCGRG 3 cut(s) 2301, 2451, 4170
AoxI GGCC 8 cut(s) 113, 524, 995, 1010, 2304, 2358, 3703, 5033
ApaLI GTGCAC 1 cut(s) 3864
ApeKI GCWGC 9 cut(s) 335, 514, 1156, 1264, 4123, 4576, 4667, 5324, 5559
AspLEI GCGC 2 cut(s) 4368, 5780
AsuC2I CCSGG 5 cut(s) 225, 1014, 4171, 4172, 4290
AsuHPI GGTGA 4 cut(s) 278, 680, 1530, 5701
AvaI CYCGRG 3 cut(s) 2301, 2451, 4170
AxyI CCTNAGG 2 cut(s) 2881, 5700
BaeGI GKGCMC 5 cut(s) 230, 1033, 3868, 4957, 5588
BaeI ACNNNNGTAYC 2 cut(s) 4467, 4500
BamHI GGATCC 1 cut(s) 175
BanI GGYRCC 3 cut(s) 227, 1030, 5585
BanII GRGCYC 1 cut(s) 4443
BarI GAAGNNNNNNTAC 8 cut(s) 1411, 1443, 2703, 2706, 2735, 2738, 2949, 2981
BauI CACGAG 1 cut(s) 6059
BbsI GAAGAC 2 cut(s) 611, 4413
Bbv12I GWGCWC 5 cut(s) 1396, 3591, 3868, 4443, 5077
BbvCI CCTCAGC 1 cut(s) 5688
BbvI GCAGC 9 cut(s) 322, 501, 1168, 1276, 4135, 4588, 4654, 5311, 5546
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 5 cut(s) 821, 1320, 3979, 4566, 5790
BciVI GTATCC 1 cut(s) 5885
BclI TGATCA 1 cut(s) 1900
BcnI CCSGG 5 cut(s) 225, 1014, 4171, 4172, 4290
BcoDI GTCTC 9 cut(s) 66, 567, 1733, 2237, 2366, 2618, 3635, 3944, 4965
BfaI CTAG 4 cut(s) 1110, 4056, 5885, 6101
BfmI CTRYAG 6 cut(s) 628, 872, 1428, 1959, 4404, 4796
BfuAI ACCTGC 4 cut(s) 192, 2309, 3048, 5783
BfuI GTATCC 1 cut(s) 5885
BglII AGATCT 3 cut(s) 3468, 3698, 5083
BlpI GCTNAGC 1 cut(s) 4068
BmcAI AGTACT 2 cut(s) 4109, 6053
BmeRI GACNNNNNGTC 1 cut(s) 1446
BmeT110I CYCGRG 3 cut(s) 2301, 2451, 4170
BmgBI CACGTC 2 cut(s) 1813, 4120
BmrI ACTGGG 2 cut(s) 1002, 4581
BmuI ACTGGG 2 cut(s) 1002, 4581
BoxI GACNNNNGTC 3 cut(s) 1929, 3909, 5252
BpiI GAAGAC 2 cut(s) 611, 4413
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 3 cut(s) 1682, 2219, 5772
Bpu10I CCTNAGC 2 cut(s) 5622, 5688
Bpu1102I GCTNAGC 1 cut(s) 4068
BpuEI CTTGAG 5 cut(s) 397, 695, 1895, 2096, 3605
BpuMI CCSGG 5 cut(s) 225, 1014, 4171, 4172, 4290
Bsa29I ATCGAT 1 cut(s) 3665
BsaAI YACGTR 1 cut(s) 2822
BsaBI GATNNNNATC 3 cut(s) 1893, 1905, 2574
BsaHI GRCGYC 2 cut(s) 1126, 3334
BsaI GGTCTC 5 cut(s) 66, 567, 1733, 2237, 3635
BsaWI WCCGGW 1 cut(s) 1333
BsaXI ACNNNNNCTCC 4 cut(s) 2217, 2247, 3248, 3278
Bse118I RCCGGY 1 cut(s) 997
Bse21I CCTNAGG 2 cut(s) 2881, 5700
Bse3DI GCAATG 6 cut(s) 206, 2105, 2145, 4323, 5060, 5750
Bse8I GATNNNNATC 3 cut(s) 1893, 1905, 2574
BseBI CCWGG 5 cut(s) 821, 1320, 3979, 4566, 5790
BseCI ATCGAT 1 cut(s) 3665
BseJI GATNNNNATC 3 cut(s) 1893, 1905, 2574
BseMI GCAATG 6 cut(s) 206, 2105, 2145, 4323, 5060, 5750
BseRI GAGGAG 6 cut(s) 29, 1733, 2459, 3582, 4010, 5688
BseSI GKGCMC 5 cut(s) 230, 1033, 3868, 4957, 5588
BseXI GCAGC 9 cut(s) 322, 501, 1168, 1276, 4135, 4588, 4654, 5311, 5546
BseYI CCCAGC 3 cut(s) 338, 2532, 5670
BsgI GTGCAG 4 cut(s) 420, 3977, 4029, 4142
Bsh1285I CGRYCG 1 cut(s) 1002
BshFI GGCC 8 cut(s) 115, 526, 997, 1012, 2306, 2360, 3705, 5035
BshNI GGYRCC 3 cut(s) 227, 1030, 5585
BshVI ATCGAT 1 cut(s) 3665
BsiEI CGRYCG 1 cut(s) 1002
BsiHKAI GWGCWC 5 cut(s) 1396, 3591, 3868, 4443, 5077
BsiHKCI CYCGRG 3 cut(s) 2301, 2451, 4170
BsiSI CCGG 7 cut(s) 224, 998, 1013, 1334, 4171, 4290, 4343
BslFI GGGAC 5 cut(s) 1058, 2045, 2468, 4582, 5240
BsmAI GTCTC 9 cut(s) 66, 567, 1733, 2237, 2366, 2618, 3635, 3944, 4965
BsmBI CGTCTC 1 cut(s) 2618
BsmFI GGGAC 5 cut(s) 1058, 2045, 2468, 4582, 5240
BsmI GAATGC 5 cut(s) 89, 4264, 4737, 4925, 5327
BsnI GGCC 8 cut(s) 115, 526, 997, 1012, 2306, 2360, 3705, 5035
Bso31I GGTCTC 5 cut(s) 66, 567, 1733, 2237, 3635
BsoBI CYCGRG 3 cut(s) 2301, 2451, 4170
Bsp1286I GDGCHC 9 cut(s) 230, 1033, 1396, 3591, 3868, 4443, 4957, 5077, 5588
Bsp1407I TGTACA 3 cut(s) 1836, 2254, 2965
Bsp1720I GCTNAGC 1 cut(s) 4068
Bsp19I CCATGG 2 cut(s) 3251, 5975
BspANI GGCC 8 cut(s) 115, 526, 997, 1012, 2306, 2360, 3705, 5035
BspDI ATCGAT 1 cut(s) 3665
BspHI TCATGA 5 cut(s) 2346, 3205, 3886, 4150, 4329
BspMAI CTGCAG 2 cut(s) 876, 1963
BspMI ACCTGC 4 cut(s) 192, 2309, 3048, 5783
BspQI GCTCTTC 5 cut(s) 542, 1943, 3207, 4448, 5622
BspT107I GGYRCC 3 cut(s) 227, 1030, 5585
BspTNI GGTCTC 5 cut(s) 66, 567, 1733, 2237, 3635
BsrDI GCAATG 6 cut(s) 206, 2105, 2145, 4323, 5060, 5750
BsrFI RCCGGY 1 cut(s) 997
BsrGI TGTACA 3 cut(s) 1836, 2254, 2965
BssAI RCCGGY 1 cut(s) 997
BssNI GRCGYC 2 cut(s) 1126, 3334
BssSI CACGAG 1 cut(s) 6059
BssT1I CCWWGG 7 cut(s) 116, 531, 1307, 3251, 5944, 5975, 6045
Bst2BI CACGAG 1 cut(s) 6059
Bst2UI CCWGG 5 cut(s) 821, 1320, 3979, 4566, 5790
BstACI GRCGYC 2 cut(s) 1126, 3334
BstAPI GCANNNNNTGC 2 cut(s) 2543, 3296
BstAUI TGTACA 3 cut(s) 1836, 2254, 2965
BstBAI YACGTR 1 cut(s) 2822
BstC8I GCNNGC 8 cut(s) 189, 1329, 1800, 1959, 5073, 5322, 5741, 6006
BstDSI CCRYGG 2 cut(s) 3251, 5975
BstENI CCTNNNNNAGG 1 cut(s) 3911
BstHHI GCGC 2 cut(s) 4368, 5780
BstMAI GTCTC 9 cut(s) 66, 567, 1733, 2237, 2366, 2618, 3635, 3944, 4965
BstMCI CGRYCG 1 cut(s) 1002
BstNI CCWGG 5 cut(s) 821, 1320, 3979, 4566, 5790
BstNSI RCATGY 8 cut(s) 649, 2701, 3112, 4207, 4964, 5728, 6008, 6012
BstPAI GACNNNNGTC 3 cut(s) 1929, 3909, 5252
BstSFI CTRYAG 6 cut(s) 628, 872, 1428, 1959, 4404, 4796
BstSLI GKGCMC 5 cut(s) 230, 1033, 3868, 4957, 5588
BstV1I GCAGC 9 cut(s) 322, 501, 1168, 1276, 4135, 4588, 4654, 5311, 5546
BstV2I GAAGAC 2 cut(s) 611, 4413
BstXI CCANNNNNNTGG 5 cut(s) 1228, 2762, 3563, 3701, 4433
Bsu15I ATCGAT 1 cut(s) 3665
Bsu36I CCTNAGG 2 cut(s) 2881, 5700
BsuI GTATCC 1 cut(s) 5885
BsuRI GGCC 8 cut(s) 115, 526, 997, 1012, 2306, 2360, 3705, 5035
BsuTUI ATCGAT 1 cut(s) 3665
BtgI CCRYGG 2 cut(s) 3251, 5975
BtrI CACGTC 2 cut(s) 1813, 4120
BtsI GCAGTG 4 cut(s) 330, 2160, 4920, 4955
BveI ACCTGC 4 cut(s) 192, 2309, 3048, 5783
Cac8I GCNNGC 8 cut(s) 189, 1329, 1800, 1959, 5073, 5322, 5741, 6006
CaiI CAGNNNCTG 1 cut(s) 4571
CciI TCATGA 5 cut(s) 2346, 3205, 3886, 4150, 4329
CfoI GCGC 2 cut(s) 4368, 5780
Cfr10I RCCGGY 1 cut(s) 997
Cfr9I CCCGGG 1 cut(s) 4170
ClaI ATCGAT 1 cut(s) 3665
CseI GACGC 2 cut(s) 1134, 3323
CspCI CAANNNNNGTGG 2 cut(s) 2350, 2385
DraIII CACNNNGTG 4 cut(s) 1391, 1799, 3143, 3281
DrdI GACNNNNNNGTC 1 cut(s) 5006
DriI GACNNNNNGTC 1 cut(s) 1446
DseDI GACNNNNNNGTC 1 cut(s) 5006
Eam1105I GACNNNNNGTC 1 cut(s) 1446
Ecl136II GAGCTC 1 cut(s) 4441
Eco130I CCWWGG 7 cut(s) 116, 531, 1307, 3251, 5944, 5975, 6045
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 4443
Eco31I GGTCTC 5 cut(s) 66, 567, 1733, 2237, 3635
Eco32I GATATC 2 cut(s) 2471, 3843
Eco53kI GAGCTC 1 cut(s) 4441
Eco57I CTGAAG 5 cut(s) 303, 551, 2048, 4429, 5112
Eco81I CCTNAGG 2 cut(s) 2881, 5700
Eco88I CYCGRG 3 cut(s) 2301, 2451, 4170
EcoICRI GAGCTC 1 cut(s) 4441
EcoNI CCTNNNNNAGG 1 cut(s) 3911
EcoO109I RGGNCCY 1 cut(s) 2455
EcoRI GAATTC 1 cut(s) 3040
EcoRII CCWGG 5 cut(s) 819, 1318, 3977, 4564, 5788
EcoRV GATATC 2 cut(s) 2471, 3843
EcoT14I CCWWGG 7 cut(s) 116, 531, 1307, 3251, 5944, 5975, 6045
EcoT22I ATGCAT 5 cut(s) 162, 1894, 3819, 5765, 6010
EcoT38I GRGCYC 1 cut(s) 4443
ErhI CCWWGG 7 cut(s) 116, 531, 1307, 3251, 5944, 5975, 6045
Esp3I CGTCTC 1 cut(s) 2618
FalI AAGNNNNNCTT 8 cut(s) 536, 568, 603, 635, 2084, 2116, 5589, 5621
FaqI GGGAC 5 cut(s) 1058, 2045, 2468, 4582, 5240
FauI CCCGC 3 cut(s) 607, 3029, 5574
FauNDI CATATG 2 cut(s) 2215, 3424
FbaI TGATCA 1 cut(s) 1900
FblI GTMKAC 2 cut(s) 2888, 5952
FriOI GRGCYC 1 cut(s) 4443
FspBI CTAG 4 cut(s) 1110, 4056, 5885, 6101
GlaI GCGC 2 cut(s) 4367, 5779
GsaI CCCAGC 3 cut(s) 342, 2536, 5674
GsuI CTGGAG 3 cut(s) 1682, 2219, 5772
HaeIII GGCC 8 cut(s) 115, 526, 997, 1012, 2306, 2360, 3705, 5035
HapII CCGG 7 cut(s) 224, 998, 1013, 1334, 4171, 4290, 4343
HgaI GACGC 2 cut(s) 1134, 3323
HhaI GCGC 2 cut(s) 4368, 5780
Hin1I GRCGYC 2 cut(s) 1126, 3334
Hin6I GCGC 2 cut(s) 4366, 5778
HinP1I GCGC 2 cut(s) 4366, 5778
HincII GTYRAC 6 cut(s) 798, 903, 3684, 4471, 5392, 5805
HindII GTYRAC 6 cut(s) 798, 903, 3684, 4471, 5392, 5805
HindIII AAGCTT 2 cut(s) 803, 6032
HpaI GTTAAC 3 cut(s) 798, 903, 5805
HpaII CCGG 7 cut(s) 224, 998, 1013, 1334, 4171, 4290, 4343
HphI GGTGA 4 cut(s) 278, 680, 1530, 5701
Hpy99I CGWCG 1 cut(s) 399
HpyCH4IV ACGT 8 cut(s) 1780, 1812, 1930, 2125, 2821, 4119, 4162, 5274
HpySE526I ACGT 8 cut(s) 1780, 1812, 1930, 2125, 2821, 4119, 4162, 5274
Hsp92I GRCGYC 2 cut(s) 1126, 3334
HspAI GCGC 2 cut(s) 4366, 5778
Ksp22I TGATCA 1 cut(s) 1900
KspAI GTTAAC 3 cut(s) 798, 903, 5805
LguI GCTCTTC 5 cut(s) 542, 1943, 3207, 4448, 5622
Lsp1109I GCAGC 9 cut(s) 322, 501, 1168, 1276, 4135, 4588, 4654, 5311, 5546
MaeI CTAG 4 cut(s) 1110, 4056, 5885, 6101
MaeII ACGT 8 cut(s) 1780, 1812, 1930, 2125, 2821, 4119, 4162, 5274
MfeI CAATTG 1 cut(s) 857
MhlI GDGCHC 9 cut(s) 230, 1033, 1396, 3591, 3868, 4443, 4957, 5077, 5588
MmeI TCCRAC 8 cut(s) 368, 1271, 1563, 1624, 2491, 3912, 5709, 5924
Mph1103I ATGCAT 5 cut(s) 162, 1894, 3819, 5765, 6010
MspA1I CMGCKG 7 cut(s) 67, 2975, 3793, 4579, 5245, 5581, 5865
MspI CCGG 7 cut(s) 224, 998, 1013, 1334, 4171, 4290, 4343
MunI CAATTG 1 cut(s) 857
Mva1269I GAATGC 5 cut(s) 89, 4264, 4737, 4925, 5327
MvaI CCWGG 5 cut(s) 821, 1320, 3979, 4566, 5790
NciI CCSGG 5 cut(s) 225, 1014, 4171, 4172, 4290
NcoI CCATGG 2 cut(s) 3251, 5975
NdeI CATATG 2 cut(s) 2215, 3424
NmeAIII GCCGAG 2 cut(s) 3139, 4912
NmuCI GTSAC 6 cut(s) 1781, 3122, 3143, 3178, 4717, 6011
NsiI ATGCAT 5 cut(s) 162, 1894, 3819, 5765, 6010
NspI RCATGY 8 cut(s) 649, 2701, 3112, 4207, 4964, 5728, 6008, 6012
OliI CACNNNNGTG 6 cut(s) 1036, 3739, 3871, 4602, 4604, 5273
PaeI GCATGC 1 cut(s) 6008
PagI TCATGA 5 cut(s) 2346, 3205, 3886, 4150, 4329
PceI AGGCCT 1 cut(s) 526
PciI ACATGT 2 cut(s) 4203, 5724
PciSI GCTCTTC 5 cut(s) 542, 1943, 3207, 4448, 5622
PcsI WCGNNNNNNNCGW 2 cut(s) 394, 1777
PctI GAATGC 5 cut(s) 89, 4264, 4737, 4925, 5327
PflMI CCANNNNNTGG 4 cut(s) 1135, 4571, 5888, 6085
PfoI TCCNGGA 1 cut(s) 4288
Ppu21I YACGTR 1 cut(s) 2822
PpuMI RGGWCCY 1 cut(s) 2455
PscI ACATGT 2 cut(s) 4203, 5724
PshAI GACNNNNGTC 3 cut(s) 1929, 3909, 5252
PsiI TTATAA 2 cut(s) 699, 1401
Psp124BI GAGCTC 1 cut(s) 4443
Psp1406I AACGTT 1 cut(s) 4162
Psp5II RGGWCCY 1 cut(s) 2455
Psp6I CCWGG 5 cut(s) 819, 1318, 3977, 4564, 5788
PspFI CCCAGC 3 cut(s) 338, 2532, 5670
PspGI CCWGG 5 cut(s) 819, 1318, 3977, 4564, 5788
PspPPI RGGWCCY 1 cut(s) 2455
PstI CTGCAG 2 cut(s) 876, 1963
PstNI CAGNNNCTG 1 cut(s) 4571
PvuII CAGCTG 3 cut(s) 3793, 4579, 5245
SacI GAGCTC 1 cut(s) 4443
SapI GCTCTTC 5 cut(s) 542, 1943, 3207, 4448, 5622
ScaI AGTACT 2 cut(s) 4109, 6053
SduI GDGCHC 9 cut(s) 230, 1033, 1396, 3591, 3868, 4443, 4957, 5077, 5588
SfcI CTRYAG 6 cut(s) 628, 872, 1428, 1959, 4404, 4796
SmaI CCCGGG 1 cut(s) 4172
SmlI CTYRAG 5 cut(s) 376, 710, 1874, 2075, 3584
SmoI CTYRAG 5 cut(s) 376, 710, 1874, 2075, 3584
SphI GCATGC 1 cut(s) 6008
SseBI AGGCCT 1 cut(s) 526
SspI AATATT 2 cut(s) 844, 1303
SspMI CTAG 4 cut(s) 1110, 4056, 5885, 6101
SstI GAGCTC 1 cut(s) 4443
StuI AGGCCT 1 cut(s) 526
StyI CCWWGG 7 cut(s) 116, 531, 1307, 3251, 5944, 5975, 6045
TaiI ACGT 8 cut(s) 1783, 1815, 1933, 2128, 2824, 4122, 4165, 5277
TaqII GACCGA 4 cut(s) 48, 438, 549, 5016
TatI WGTACW 8 cut(s) 78, 876, 1836, 2254, 2965, 3787, 4107, 6051
TauI GCSGC 2 cut(s) 940, 1691
TseFI GTSAC 6 cut(s) 1781, 3122, 3143, 3178, 4717, 6011
TseI GCWGC 9 cut(s) 335, 514, 1156, 1264, 4123, 4576, 4667, 5324, 5559
Tsp45I GTSAC 6 cut(s) 1781, 3122, 3143, 3178, 4717, 6011
TspGWI ACGGA 6 cut(s) 821, 1786, 2942, 3851, 4118, 4530
TspMI CCCGGG 1 cut(s) 4170
Van91I CCANNNNNTGG 4 cut(s) 1135, 4571, 5888, 6085
VneI GTGCAC 1 cut(s) 3864
XagI CCTNNNNNAGG 1 cut(s) 3911
XceI RCATGY 8 cut(s) 649, 2701, 3112, 4207, 4964, 5728, 6008, 6012
XcmI CCANNNNNNNNNTGG 2 cut(s) 3509, 5028
XmaI CCCGGG 1 cut(s) 4170
XmiI GTMKAC 2 cut(s) 2888, 5952
XspI CTAG 4 cut(s) 1110, 4056, 5885, 6101
ZrmI AGTACT 2 cut(s) 4109, 6053
Zsp2I ATGCAT 5 cut(s) 162, 1894, 3819, 5765, 6010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.