Rh2AG549500

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000798
Physical Location & Seq
Forward (+)
77988303 .. 77999577
11275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG549500.1

Sequence Viewer

Length: 5496 bp
ATGTGGTACAACGCCGAAATTTCACCGCCGGGGACGGGAAATACCTCGATTCCATCGGCGGTGGCTCAGCTCTGTCTTCCGTCACCGGCCGATCTTAAAGAGAAAGCCCGCAGATCGCAGCAGCTTTCCTCCCGCAAGTGGTCCAATCGCCACGGCGAACGGCGCGCCGTCGATTCGCAAAAACAGGACATGCCTCCGGAGCATGTGAGGAAGGTCGTGAAAGAGCACGGCGACATGTCGTCGAAGAAGTACCGGCACGACAAGCGGGTCTACCTGGGAGCTCTGAAGTTCGTTCCTCACGCCGTGTACAAGCTTCTCGAGAATATGCCGATGCCGTGGGAGCAGGTCCGTAATGTGAAGGTGCTGTACCATATTACCGGCGCAGTTACCTTCGTCAACGAGACTCCCTGGGTTGTGGAGCCGATCTACATGGCTCAATGGGCCACAATGTGGATCATGATGAGGAGGGAGAAGAGGGATCGCCGGCATTTCAAGAGGATGAGGTTTCCGCCGTTCGATGATGAGGAGCCTATGTTGGATTATGCTGATAACTTGTTGGATGTTGAGCCTCTTGAGCCGATTCAGCTCGAGCTTGATGCCAAAGAGGATTCGGCTGTGTATGACTGGTTTTATGACCATAAACCTCTTGTGAAGACAAAGCTGATCAACGGCCCGACCTACCGGAAGTGGCAGCTCTCCCCGCCGATTATGGAAGCCCTTTACCGGCTTTCAGGACAGGTAATTTCCGATTTGACTGATCGGAACTATTTCTATTTGTTTGATAAGGAGTCGTTTCTGACAGCCAAGGCATTGAATATGTGTATACCAGGTGGGCCGAAGTTCGAGCCATTGTTTAGGGATATGCAGCAAGGGGAGGAGGATTGGAATGAGTTTAATGACATAAACAAGCTCATTATTCGGTCTCCGCTGAGATCAGAGTACAAAATTGCATTCCCTAATCTTTACAACAACAGGCCAAGGAAGGTAAAGCTCTCTGTTTATCACACACCAACGGTTATGCATATTAAAACTGAGGATCCTGACCTGCCTGCATTTTATTATGATCCATTGCTAAACCCAATACCAGGCACCAACAGGAGTAGTTATGATAATAGTGAGGAAGATGGTGATTTTGTTTTGCCTGAAGGGGTGGAGCCTCTTTTGAGTCATACTCAGCTTTATACTGACACCACTGCTGCTGGGATTTCCCTGTATTTTGCCCCACGCCCTTTTAACTTGAGGTCTGGTCGGACTTGTCGCGCAGAGGATATACCTCTTGTGTCAGAGTGGTTTAAGGAGCATTGTCCTCAGTCATATCCTGTTAAGGTTCGAGTCAGCTGTCAGAAGTTGTTGAAATCGTTTGTGTTGAATGAGCTGCATCGTAGGCCTCCCAGGGCACAAAAGAAGAAGAACTTGTTTCGGTCTCTTCAGGCTACCAAGTTTTTCCAAACTACTGAGCTTGATTGGGTTGAAGCAGGTCTTCAACTCTGTAGGCAGGGTCATAACATGCTAAATCTGCTGATACATAGGAAAGGTTTGAACTATCTTCACCTTGATTATAATTTTAATCTCAAGCCTGTGAAAACCCTGACAACAAAAGAGCGTAAGAAATCTCGGTTTGGTAATCCTTTTCATCTGTTGCGTGAAATCTTGCGGTTAACAAAGCTTGTAGTTGATGCCCAGGTCCAGTTCCGTTTGGGTAATATTGATGCCTTTCAATTGGCTGATGGTCTGCAGTACATATTTTCTCATGTTGGTCAGTTAACTGGAATGTACCGTTACAAGTATAGGTTGATGCGGCAGATTAGAATGAGCAAAGATTTGAAGCACTTGATTTACTACCGTTTCAATACTGGGCCGGTCGGGAAAGGGCCGGGATGTGGATTCTGGGCACCAATGTGGAGGGTGTGGTTGTCCTTCCTTCGTGGCATGGTCCCTCTTCTGGAACAGTGGTTGGGAAATTTACTTGCTAGACAATTTGAAGGGCGCCATTCTGTGGCAAAGACAGTAACTAAGCAGAGTGTTGAAAGCAATTTCGACTTGGAGTTTCGAGCAGATGTCTTGAAAGATATTCTGGATGCCATGCCAGTGGGCATGAAGCAAAATAAGGCGAAAACAATTATGCAGCATCTCAGTGAGGCATGGCGTTGTTGGAAAGCAAATATTCCTTGGAAGGTTCCTGCAGTAAAATTGAACCAGCATCAAAGAGAGGAGTTGGGCCTCATTGAACAAGCCTATGATAACCCCCATGAGGCATTGATACGGATAAAACGTCACTTGATCTCACAGCGTGCCTTCAAAGACGTGAGCATAGAGTTCATGGATCTGTACAGCCATCATATTCCAGTTTTTGAGATTGACCCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGCTGACAAACGTCAGCTCTTCCCGAACTGGATCAAGCCTGCAGACTCAGAGCCACCTCCACTTTTGGTCTATAAATGGTGTCAGGGGATAAACAATTTAGAGAGTATATGGGACACAAGTGAGGGGGAGTGTGTTGTGATGCTTCAGACAAAACTTGAGAGATTATCTGAGAAGATTGACTTGACATTGCTAAATAGGCTTCTACGTCTTATTCTTGACCATAACATTGCTGATTATATCACTGCAAAAAACAATGTGGTATTGTCTTACAAAGATATGAGTCATACAAATTCATATGGTCTTATACGGGGTCTCCAGCCTGCATCATTTGTTGTACAGTATTATGGGCTTGTTTTGGATCTAATGCTTCTTGGATTGACTTGGGCCAATGAAATTGCAGGTCCACAGCAGAAGCCAAATGAGTTCATGACTTATGGGGCCACAAAGGTTGAGACTCGCCATCCTATCAGGCTGTACTCTCGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACTCAGGAGGAGGCTCGGGACCTCATACAGCGATATCTTACTGAGCATCCAGATCCTAATAATGAAAATATGGTTGGATATAATAATAAGAAGTGCTGGGCAAGAGATGCGAGGATGAGGCTCCTGAAACATGATGTCAATCTTGGAAGGAGTGTCTTTTGGAATGTCAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAATGGGAGGACAGTTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTTTTCAGCATGTGTGGATTTGAAGTAAGAATACTTCCCAAGATAAGAATGAGTACGAAGGAACACCAAAGAGATGGAGTTTGGAACTTGCAAAATGAACAGACAAAGGAAAGGACTGCTGTTGCTTTTTTACGTGTTGAGGATGAATACATGAAGGCGTTTGAGAATCGTGCAAGGCAGATCCTTATGTCCTCAGGGTCTACAACATTCACAAAAGTTATCAACAAATGGAATACTGCCCTTATTGGTCTTATGACTTACTTCCGTGAAGCAGTTGTACATACAGCGGAGCTGTTAGATTTACTGGTCAAATGTGAAAATAAAATTCAGACCCGCATTAAGATTGGATTGAATTCAAAGATGCCAAGCAGGTTCCCACCTGTCATTTTTTACACGCCAAAAGAAATTGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAGAGTGACCTCAGATACTCTCGGCAGACAGAAATTGGTGTGACACACTTTAGGAGTGGAATGAGTCATGAAGAAGAGCAGCTGATTCCAAATCTTTACCGTTACATACAACCATGGGAGAGTGAGTTTATAGATTCACAGCGTGTTTGGGCAGAATATGCACTAAAAAGACAGGAAACTATGGAACAGAATCGGCGTCTTACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACATTGTTCCAGAAGGATCGACACACTTCAGCATATGACAAAGGGTGGAGAGTTCGCACAGATTTTAAGCAGTACCAGATCTTGAAGCAAAATCCTTTCTGGTGGACACACCAAAGGCATGATGGAAAATTGTGGAATTTGAATAATTATAGAACTGATGTGATCCAAGCTCTTGGAGGAGTTGAGGGAATACTTGAGCACACCTTATTCAAAGGAACATACTTCCCTACTTGGGAGGGTCTCTTTTGGGAGAAGGCATCTGGATTTGAGGAATCGATGAAGTATAAAAAGTTGACTAATGCCCAAAGATCTGGCCTCAACCAAATCCCCAATCGTAGGTTTGCACTTTGGTGGTCGCCTACCATAAATCGAGCCAATGTATATGTCGGTTTTCAAGTACAGCTGGATCTGACAGGAATCTTTATGCATGGGAAGATCCCAACCTTGAAGATATCGTTGATTCAGATATTCCGTGCACACTTGTGGCAGAAGATTCATGAAAGTGTTGTGATGGATCTTTGTCAGGTTTTGGATCAGGAGTTGGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACCATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCAGACATTCTCCTCTTTTCTGCTCATAAATGGCAAATGTCGAAACCTAGTCTTGTGGCTGAGCCGAAGGATGAGTATGATAAAAAAACAAGCAACAAGTACTGGATAGACGTGCAGCTCCGTTGGGGGGATTATGATTCTCATGATATTGAACGTTACACCCGGGCAAAGTTTATGGACTATACAACTGATAACATGTCTATCTATCCTTCTCCGACGGGAGTGATGATTGGTATTGATTTAGCCTATAATGTGCATTCTGCATTTGGTAACTGGTTTCCCGGATCAAAACCACTGATTCAACAGGCAATGAACAAGATCATGAAGTCAAATCCGGCTTTATATGTGTTGAGGGAGCGCATAAGGAAAGGATTGCAGTTGTATTCCTCGGAGCCTACAGAGCCATATTTGTCTTCCCAAAACTATGGGGAGCTCTTCAGCAATCAAATTAAATGGTTTGTTGACGATAAAAATGTGTATCGTGTTACAATGCACAAGACTTTTGACGGAAATCTTGTTACAAAACCCATCAATGGTGCTATCTTCATGTTCAATCCAGGGACTGGGCAGCTGTTCTTGAAGGTTATCCACACAAGTGTGTGGGCAGGACAGAAGCGACTTGGGCAGTTGGCTAAATGGAAAACTGCAGAAGAAGTTGCTGCTCTTGTGCGTTCTCTACCTGTTGAAGAACAGCCAAAGCAAATTATTGTGACTCGTAAGGGAATGCTCGACCCATTGGAGGTTCATTTGCTGGATTTTCCCAACATAGTCATAAAAGGAAGTGAGCTACAGCTTCCTTTTCAGGTTTGCTTGAAAATTGAGAAGTTGAATGATCTGGTACTAAAGTCTACTGAGCCACAGATGGTTCTTTTTAATGTTTACGATGATTGGTTGAAGAGTGTTTCATCATACACTGCATTCTCTAGGCTCATTCTGATTCTGCGGGCACTGCATGTGAACAGAGACAAGGCAAGTATGTTGTTGAAGCCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

1831

Amino Acids

214.45

Weight (kDa)

9.19

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 61 - 212 1.3e-83 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 396 - 729 6e-183 PROCN (NUC071) domain
RRM_4 PF10598 861 - 950 1.1e-43 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1111 - 1246 2.2e-74 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1316 - 1474 7.3e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1634 - 1831 3.9e-110 PRP8 domain IV core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1560
AasI GACNNNNNNGTC 1 cut(s) 266
Acc36I ACCTGC 5 cut(s) 334, 1053, 1466, 2801, 3540
AccB1I GGYRCC 3 cut(s) 1088, 1891, 1986
AccB7I CCANNNNNTGG 3 cut(s) 450, 1996, 5063
AccI GTMKAC 4 cut(s) 270, 823, 3380, 5348
AccII CGCG 2 cut(s) 165, 1260
AccIII TCCGGA 1 cut(s) 196
AclI AACGTT 1 cut(s) 4654
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 7 cut(s) 18, 1960, 2701, 3175, 3504, 3532, 4018
AcuI CTGAAG 6 cut(s) 305, 1164, 1412, 2540, 3894, 4921
AcyI GRCGYC 2 cut(s) 1987, 3826
AdeI CACNNNGTG 5 cut(s) 304, 450, 2291, 3635, 3773
AflIII ACRYGT 3 cut(s) 234, 3313, 4695
AhdI GACNNNNNGTC 1 cut(s) 238
AjiI CACGTC 2 cut(s) 2305, 4612
AjnI CCWGG 8 cut(s) 273, 407, 826, 1084, 1391, 1680, 4469, 5056
AjuI GAANNNNNNNTTGG 2 cut(s) 2985, 3017
AleI CACNNNNGTG 5 cut(s) 1897, 4231, 4363, 5094, 5096
Alw21I GWGCWC 5 cut(s) 228, 283, 4083, 4360, 4935
Alw26I GTCTC 9 cut(s) 395, 927, 1428, 2729, 2858, 3110, 4127, 4436, 5457
Alw44I GTGCAC 1 cut(s) 4356
AlwNI CAGNNNCTG 1 cut(s) 5063
Ama87I CYCGRG 4 cut(s) 317, 587, 2943, 4662
Aor13HI TCCGGA 1 cut(s) 196
ApaLI GTGCAC 1 cut(s) 4356
ApoI RAATTY 7 cut(s) 18, 1960, 2701, 3175, 3504, 3532, 4018
AscI GGCGCGCC 1 cut(s) 163
Asp700I GAANNNNTTC 1 cut(s) 767
AspLEI GCGC 6 cut(s) 165, 167, 383, 1262, 1989, 4860
AsuC2I CCSGG 5 cut(s) 30, 1875, 4663, 4664, 4782
AsuHPI GGTGA 4 cut(s) 15, 75, 1139, 1541
AvaI CYCGRG 4 cut(s) 317, 587, 2943, 4662
AvaII GGWCC 6 cut(s) 141, 346, 1684, 1933, 2813, 2947
AxyI CCTNAGG 1 cut(s) 3373
BaeGI GKGCMC 4 cut(s) 1399, 1894, 4360, 5449
BaeI ACNNNNGTAYC 2 cut(s) 5330, 5363
BamHI GGATCC 1 cut(s) 1036
BanI GGYRCC 3 cut(s) 1088, 1891, 1986
BanII GRGCYC 2 cut(s) 283, 4935
BarI GAAGNNNNNNTAC 8 cut(s) 350, 382, 3195, 3198, 3227, 3230, 3441, 3473
BbsI GAAGAC 4 cut(s) 68, 659, 1472, 4905
Bbv12I GWGCWC 5 cut(s) 228, 283, 4083, 4360, 4935
BceAI ACGGC 8 cut(s) 152, 169, 176, 244, 287, 319, 496, 685
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 8 cut(s) 275, 409, 828, 1086, 1393, 1682, 4471, 5058
BclI TGATCA 2 cut(s) 663, 2392
BcnI CCSGG 5 cut(s) 30, 1875, 4663, 4664, 4782
BcoDI GTCTC 9 cut(s) 395, 927, 1428, 2729, 2858, 3110, 4127, 4436, 5457
BfaI CTAG 3 cut(s) 1971, 4548, 5424
BfmI CTRYAG 7 cut(s) 1489, 1733, 2181, 2451, 4896, 5145, 5288
BfoI RGCGCY 1 cut(s) 1990
BfuAI ACCTGC 5 cut(s) 334, 1053, 1466, 2801, 3540
BglII AGATCT 2 cut(s) 3960, 4190
BlpI GCTNAGC 2 cut(s) 66, 4560
BmcAI AGTACT 1 cut(s) 4601
Bme18I GGWCC 6 cut(s) 141, 346, 1684, 1933, 2813, 2947
BmeRI GACNNNNNGTC 1 cut(s) 238
BmeT110I CYCGRG 4 cut(s) 317, 587, 2943, 4662
BmgBI CACGTC 2 cut(s) 2305, 4612
BmrI ACTGGG 2 cut(s) 1863, 5073
BmuI ACTGGG 2 cut(s) 1863, 5073
BoxI GACNNNNGTC 1 cut(s) 2421
BpiI GAAGAC 4 cut(s) 68, 659, 1472, 4905
BplI GAGNNNNNCTC 2 cut(s) 1156, 1188
BpmI CTGGAG 1 cut(s) 2711
Bpu1102I GCTNAGC 2 cut(s) 66, 4560
BpuEI CTTGAG 6 cut(s) 593, 1258, 1556, 2387, 2588, 4097
BpuMI CCSGG 5 cut(s) 30, 1875, 4663, 4664, 4782
Bsa29I ATCGAT 1 cut(s) 4157
BsaAI YACGTR 1 cut(s) 3314
BsaBI GATNNNNATC 3 cut(s) 2385, 2397, 3066
BsaHI GRCGYC 2 cut(s) 1987, 3826
BsaI GGTCTC 4 cut(s) 927, 1428, 2729, 4127
BsaWI WCCGGW 2 cut(s) 196, 681
BsaXI ACNNNNNCTCC 6 cut(s) 518, 548, 2709, 2739, 3740, 3770
Bse118I RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
Bse21I CCTNAGG 1 cut(s) 3373
Bse3DI GCAATG 4 cut(s) 1067, 2597, 2637, 4815
Bse8I GATNNNNATC 3 cut(s) 2385, 2397, 3066
BseAI TCCGGA 1 cut(s) 196
BseBI CCWGG 8 cut(s) 275, 409, 828, 1086, 1393, 1682, 4471, 5058
BseCI ATCGAT 1 cut(s) 4157
BseJI GATNNNNATC 3 cut(s) 2385, 2397, 3066
BseMI GCAATG 4 cut(s) 1067, 2597, 2637, 4815
BsePI GCGCGC 1 cut(s) 163
BseRI GAGGAG 7 cut(s) 478, 539, 890, 2225, 2951, 4074, 4502
BseSI GKGCMC 4 cut(s) 1399, 1894, 4360, 5449
BseX3I CGGCCG 1 cut(s) 87
BseYI CCCAGC 2 cut(s) 1199, 3024
BsgI GTGCAG 3 cut(s) 4469, 4521, 4634
Bsh1236I CGCG 2 cut(s) 165, 1260
Bsh1285I CGRYCG 2 cut(s) 90, 1863
BshNI GGYRCC 3 cut(s) 1088, 1891, 1986
BshVI ATCGAT 1 cut(s) 4157
BsiEI CGRYCG 2 cut(s) 90, 1863
BsiHKAI GWGCWC 5 cut(s) 228, 283, 4083, 4360, 4935
BsiHKCI CYCGRG 4 cut(s) 317, 587, 2943, 4662
BslFI GGGAC 5 cut(s) 46, 1919, 2537, 2960, 5074
BsmAI GTCTC 9 cut(s) 395, 927, 1428, 2729, 2858, 3110, 4127, 4436, 5457
BsmBI CGTCTC 1 cut(s) 3110
BsmFI GGGAC 5 cut(s) 46, 1919, 2537, 2960, 5074
BsmI GAATGC 4 cut(s) 950, 4756, 5229, 5417
Bso31I GGTCTC 4 cut(s) 927, 1428, 2729, 4127
BsoBI CYCGRG 4 cut(s) 317, 587, 2943, 4662
Bsp1286I GDGCHC 8 cut(s) 228, 283, 1399, 1894, 4083, 4360, 4935, 5449
Bsp13I TCCGGA 1 cut(s) 196
Bsp1407I TGTACA 4 cut(s) 306, 2328, 2746, 3457
Bsp1720I GCTNAGC 2 cut(s) 66, 4560
Bsp19I CCATGG 1 cut(s) 3743
BspDI ATCGAT 1 cut(s) 4157
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 2 cut(s) 165, 1260
BspHI TCATGA 6 cut(s) 456, 2838, 3697, 4378, 4642, 4821
BspMAI CTGCAG 4 cut(s) 1737, 2185, 2455, 5149
BspMI ACCTGC 5 cut(s) 334, 1053, 1466, 2801, 3540
BspQI GCTCTTC 3 cut(s) 2435, 3699, 4940
BspT107I GGYRCC 3 cut(s) 1088, 1891, 1986
BspTNI GGTCTC 4 cut(s) 927, 1428, 2729, 4127
BsrDI GCAATG 4 cut(s) 1067, 2597, 2637, 4815
BsrFI RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
BsrGI TGTACA 4 cut(s) 306, 2328, 2746, 3457
BssAI RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
BssHII GCGCGC 1 cut(s) 163
BssNAI GTATAC 1 cut(s) 824
BssNI GRCGYC 2 cut(s) 1987, 3826
BssT1I CCWWGG 4 cut(s) 804, 977, 2168, 3743
Bst1107I GTATAC 1 cut(s) 824
Bst2UI CCWGG 8 cut(s) 275, 409, 828, 1086, 1393, 1682, 4471, 5058
Bst6I CTCTTC 8 cut(s) 467, 1431, 1944, 2435, 3699, 4469, 4940, 5390
BstACI GRCGYC 2 cut(s) 1987, 3826
BstAPI GCANNNNNTGC 2 cut(s) 3035, 3788
BstAUI TGTACA 4 cut(s) 306, 2328, 2746, 3457
BstBAI YACGTR 1 cut(s) 3314
BstC8I GCNNGC 8 cut(s) 109, 165, 485, 1050, 2292, 2451, 2733, 5445
BstDSI CCRYGG 3 cut(s) 151, 335, 3743
BstFNI CGCG 2 cut(s) 165, 1260
BstH2I RGCGCY 1 cut(s) 1990
BstHHI GCGC 6 cut(s) 165, 167, 383, 1262, 1989, 4860
BstMAI GTCTC 9 cut(s) 395, 927, 1428, 2729, 2858, 3110, 4127, 4436, 5457
BstMCI CGRYCG 2 cut(s) 90, 1863
BstNI CCWGG 8 cut(s) 275, 409, 828, 1086, 1393, 1682, 4471, 5058
BstNSI RCATGY 8 cut(s) 193, 206, 238, 1510, 3193, 3604, 4699, 5456
BstPAI GACNNNNGTC 1 cut(s) 2421
BstSFI CTRYAG 7 cut(s) 1489, 1733, 2181, 2451, 4896, 5145, 5288
BstSLI GKGCMC 4 cut(s) 1399, 1894, 4360, 5449
BstUI CGCG 2 cut(s) 165, 1260
BstV2I GAAGAC 4 cut(s) 68, 659, 1472, 4905
BstXI CCANNNNNNTGG 5 cut(s) 2089, 3254, 4055, 4193, 4925
BstZ17I GTATAC 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 4157
Bsu36I CCTNAGG 1 cut(s) 3373
BsuTUI ATCGAT 1 cut(s) 4157
BtgI CCRYGG 3 cut(s) 151, 335, 3743
BtrI CACGTC 2 cut(s) 2305, 4612
BtsI GCAGTG 4 cut(s) 1191, 2652, 5412, 5447
BtsIMutI CAGTG 8 cut(s) 1191, 1955, 2094, 2140, 2652, 4793, 5412, 5447
BveI ACCTGC 5 cut(s) 334, 1053, 1466, 2801, 3540
Cac8I GCNNGC 8 cut(s) 109, 165, 485, 1050, 2292, 2451, 2733, 5445
CaiI CAGNNNCTG 1 cut(s) 5063
CciI TCATGA 6 cut(s) 456, 2838, 3697, 4378, 4642, 4821
CfoI GCGC 6 cut(s) 165, 167, 383, 1262, 1989, 4860
Cfr10I RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
Cfr9I CCCGGG 1 cut(s) 4662
ClaI ATCGAT 1 cut(s) 4157
CseI GACGC 1 cut(s) 3815
CsiI ACCWGGT 1 cut(s) 826
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
DinI GGCGCC 1 cut(s) 1988
DraIII CACNNNGTG 5 cut(s) 304, 450, 2291, 3635, 3773
DrdI GACNNNNNNGTC 1 cut(s) 266
DriI GACNNNNNGTC 1 cut(s) 238
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 8 cut(s) 467, 1431, 1944, 2435, 3699, 4469, 4940, 5390
Eam1105I GACNNNNNGTC 1 cut(s) 238
EarI CTCTTC 8 cut(s) 467, 1431, 1944, 2435, 3699, 4469, 4940, 5390
EciI GGCGGA 1 cut(s) 498
Ecl136II GAGCTC 2 cut(s) 281, 4933
EclXI CGGCCG 1 cut(s) 87
Eco130I CCWWGG 4 cut(s) 804, 977, 2168, 3743
Eco147I AGGCCT 1 cut(s) 1387
Eco24I GRGCYC 2 cut(s) 283, 4935
Eco31I GGTCTC 4 cut(s) 927, 1428, 2729, 4127
Eco32I GATATC 2 cut(s) 2963, 4335
Eco47I GGWCC 6 cut(s) 141, 346, 1684, 1933, 2813, 2947
Eco52I CGGCCG 1 cut(s) 87
Eco53kI GAGCTC 2 cut(s) 281, 4933
Eco57I CTGAAG 6 cut(s) 305, 1164, 1412, 2540, 3894, 4921
Eco81I CCTNAGG 1 cut(s) 3373
Eco88I CYCGRG 4 cut(s) 317, 587, 2943, 4662
EcoICRI GAGCTC 2 cut(s) 281, 4933
EcoO109I RGGNCCY 1 cut(s) 2947
EcoRI GAATTC 1 cut(s) 3532
EcoRII CCWGG 8 cut(s) 273, 407, 826, 1084, 1391, 1680, 4469, 5056
EcoRV GATATC 2 cut(s) 2963, 4335
EcoT14I CCWWGG 4 cut(s) 804, 977, 2168, 3743
EcoT22I ATGCAT 3 cut(s) 1023, 2386, 4311
EcoT38I GRGCYC 2 cut(s) 283, 4935
EgeI GGCGCC 1 cut(s) 1988
EheI GGCGCC 1 cut(s) 1988
ErhI CCWWGG 4 cut(s) 804, 977, 2168, 3743
Esp3I CGTCTC 1 cut(s) 3110
FalI AAGNNNNNCTT 6 cut(s) 1397, 1429, 1464, 1496, 2576, 2608
FaqI GGGAC 5 cut(s) 46, 1919, 2537, 2960, 5074
FauI CCCGC 6 cut(s) 116, 140, 258, 708, 3521, 5436
FauNDI CATATG 2 cut(s) 2707, 3916
FbaI TGATCA 2 cut(s) 663, 2392
FblI GTMKAC 4 cut(s) 270, 823, 3380, 5348
FriOI GRGCYC 2 cut(s) 283, 4935
FspBI CTAG 3 cut(s) 1971, 4548, 5424
GlaI GCGC 6 cut(s) 164, 166, 382, 1261, 1988, 4859
GsaI CCCAGC 2 cut(s) 1203, 3028
GsuI CTGGAG 1 cut(s) 2711
HaeII RGCGCY 1 cut(s) 1990
HgaI GACGC 1 cut(s) 3815
HhaI GCGC 6 cut(s) 165, 167, 383, 1262, 1989, 4860
Hin1I GRCGYC 2 cut(s) 1987, 3826
Hin6I GCGC 6 cut(s) 163, 165, 381, 1260, 1987, 4858
HinP1I GCGC 6 cut(s) 163, 165, 381, 1260, 1987, 4858
HincII GTYRAC 5 cut(s) 397, 1659, 1764, 4176, 4963
HindII GTYRAC 5 cut(s) 397, 1659, 1764, 4176, 4963
HindIII AAGCTT 2 cut(s) 311, 1664
HpaI GTTAAC 2 cut(s) 1659, 1764
HphI GGTGA 4 cut(s) 15, 75, 1139, 1541
Hpy99I CGWCG 3 cut(s) 173, 244, 4720
HpyCH4IV ACGT 7 cut(s) 2272, 2304, 2422, 2617, 3313, 4611, 4654
HpySE526I ACGT 7 cut(s) 2272, 2304, 2422, 2617, 3313, 4611, 4654
Hsp92I GRCGYC 2 cut(s) 1987, 3826
HspAI GCGC 6 cut(s) 163, 165, 381, 1260, 1987, 4858
KasI GGCGCC 1 cut(s) 1986
Kpn2I TCCGGA 1 cut(s) 196
KroI GCCGGC 1 cut(s) 483
KroNI GCCGGC 1 cut(s) 485
Ksp22I TGATCA 2 cut(s) 663, 2392
KspAI GTTAAC 2 cut(s) 1659, 1764
LguI GCTCTTC 3 cut(s) 2435, 3699, 4940
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 3 cut(s) 1971, 4548, 5424
MaeII ACGT 7 cut(s) 2272, 2304, 2422, 2617, 3313, 4611, 4654
MfeI CAATTG 1 cut(s) 1718
MhlI GDGCHC 8 cut(s) 228, 283, 1399, 1894, 4083, 4360, 4935, 5449
Mly113I GGCGCC 1 cut(s) 1987
MlyI GAGTC 9 cut(s) 397, 797, 1174, 1341, 2450, 2701, 2860, 3703, 5206
MmeI TCCRAC 7 cut(s) 516, 537, 1229, 2132, 2983, 4404, 4739
Mph1103I ATGCAT 3 cut(s) 1023, 2386, 4311
MroI TCCGGA 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 767
MslI CAYNNNNRTG 8 cut(s) 1897, 2087, 2133, 4231, 4363, 4383, 5094, 5096
MspA1I CMGCKG 6 cut(s) 928, 1338, 3467, 3712, 4285, 5071
MunI CAATTG 1 cut(s) 1718
Mva1269I GAATGC 4 cut(s) 950, 4756, 5229, 5417
MvaI CCWGG 8 cut(s) 275, 409, 828, 1086, 1393, 1682, 4471, 5058
MvnI CGCG 2 cut(s) 165, 1260
NaeI GCCGGC 1 cut(s) 485
NarI GGCGCC 1 cut(s) 1987
NciI CCSGG 5 cut(s) 30, 1875, 4663, 4664, 4782
NcoI CCATGG 1 cut(s) 3743
NdeI CATATG 2 cut(s) 2707, 3916
NgoMIV GCCGGC 1 cut(s) 483
NmeAIII GCCGAG 1 cut(s) 3631
NmuCI GTSAC 6 cut(s) 81, 2273, 3614, 3635, 3670, 5209
NsiI ATGCAT 3 cut(s) 1023, 2386, 4311
NspI RCATGY 8 cut(s) 193, 206, 238, 1510, 3193, 3604, 4699, 5456
OliI CACNNNNGTG 5 cut(s) 1897, 4231, 4363, 5094, 5096
PaeR7I CTCGAG 2 cut(s) 317, 587
PagI TCATGA 6 cut(s) 456, 2838, 3697, 4378, 4642, 4821
PalAI GGCGCGCC 1 cut(s) 163
PasI CCCWGGG 2 cut(s) 408, 1392
PauI GCGCGC 1 cut(s) 163
PceI AGGCCT 1 cut(s) 1387
PciI ACATGT 2 cut(s) 234, 4695
PciSI GCTCTTC 3 cut(s) 2435, 3699, 4940
PcsI WCGNNNNNNNCGW 1 cut(s) 2269
PctI GAATGC 4 cut(s) 950, 4756, 5229, 5417
PdiI GCCGGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 767
PflMI CCANNNNNTGG 3 cut(s) 450, 1996, 5063
PfoI TCCNGGA 1 cut(s) 4780
PleI GAGTC 9 cut(s) 397, 796, 1173, 1340, 2450, 2700, 2860, 3702, 5206
PluTI GGCGCC 1 cut(s) 1990
PpsI GAGTC 9 cut(s) 397, 796, 1173, 1340, 2450, 2700, 2860, 3702, 5206
Ppu21I YACGTR 1 cut(s) 3314
PpuMI RGGWCCY 1 cut(s) 2947
PscI ACATGT 2 cut(s) 234, 4695
PshAI GACNNNNGTC 1 cut(s) 2421
PsiI TTATAA 1 cut(s) 1560
Psp124BI GAGCTC 2 cut(s) 283, 4935
Psp1406I AACGTT 1 cut(s) 4654
Psp5II RGGWCCY 1 cut(s) 2947
Psp6I CCWGG 8 cut(s) 273, 407, 826, 1084, 1391, 1680, 4469, 5056
PspFI CCCAGC 2 cut(s) 1199, 3024
PspGI CCWGG 8 cut(s) 273, 407, 826, 1084, 1391, 1680, 4469, 5056
PspPPI RGGWCCY 1 cut(s) 2947
PspXI VCTCGAGB 1 cut(s) 587
PstI CTGCAG 4 cut(s) 1737, 2185, 2455, 5149
PstNI CAGNNNCTG 1 cut(s) 5063
PteI GCGCGC 1 cut(s) 163
PvuII CAGCTG 4 cut(s) 1338, 3712, 4285, 5071
RseI CAYNNNNRTG 8 cut(s) 1897, 2087, 2133, 4231, 4363, 4383, 5094, 5096
SacI GAGCTC 2 cut(s) 283, 4935
SapI GCTCTTC 3 cut(s) 2435, 3699, 4940
ScaI AGTACT 1 cut(s) 4601
SchI GAGTC 9 cut(s) 397, 797, 1174, 1341, 2450, 2701, 2860, 3703, 5206
SduI GDGCHC 8 cut(s) 228, 283, 1399, 1894, 4083, 4360, 4935, 5449
SexAI ACCWGGT 1 cut(s) 826
SfcI CTRYAG 7 cut(s) 1489, 1733, 2181, 2451, 4896, 5145, 5288
SfoI GGCGCC 1 cut(s) 1988
Sfr274I CTCGAG 2 cut(s) 317, 587
SgsI GGCGCGCC 1 cut(s) 163
SinI GGWCC 6 cut(s) 141, 346, 1684, 1933, 2813, 2947
SlaI CTCGAG 2 cut(s) 317, 587
SmaI CCCGGG 1 cut(s) 4664
SmiMI CAYNNNNRTG 8 cut(s) 1897, 2087, 2133, 4231, 4363, 4383, 5094, 5096
SmlI CTYRAG 8 cut(s) 317, 572, 587, 1237, 1571, 2366, 2567, 4076
SmoI CTYRAG 8 cut(s) 317, 572, 587, 1237, 1571, 2366, 2567, 4076
SseBI AGGCCT 1 cut(s) 1387
SspDI GGCGCC 1 cut(s) 1986
SspI AATATT 2 cut(s) 1705, 2164
SspMI CTAG 3 cut(s) 1971, 4548, 5424
SstI GAGCTC 2 cut(s) 283, 4935
StuI AGGCCT 1 cut(s) 1387
StyI CCWWGG 4 cut(s) 804, 977, 2168, 3743
TaiI ACGT 7 cut(s) 2275, 2307, 2425, 2620, 3316, 4614, 4657
TaqII GACCGA 2 cut(s) 909, 1410
TatI WGTACW 9 cut(s) 306, 939, 1737, 2328, 2746, 2886, 3457, 4279, 4599
TauI GCSGC 1 cut(s) 1801
TscAI CASTG 8 cut(s) 1198, 1955, 2094, 2140, 2659, 4800, 5419, 5454
TseFI GTSAC 6 cut(s) 81, 2273, 3614, 3635, 3670, 5209
Tsp45I GTSAC 6 cut(s) 81, 2273, 3614, 3635, 3670, 5209
TspGWI ACGGA 8 cut(s) 69, 338, 1682, 2278, 3434, 4343, 4610, 5022
TspMI CCCGGG 1 cut(s) 4662
TspRI CASTG 8 cut(s) 1198, 1955, 2094, 2140, 2659, 4800, 5419, 5454
Van91I CCANNNNNTGG 3 cut(s) 450, 1996, 5063
VneI GTGCAC 1 cut(s) 4356
VpaK11BI GGWCC 6 cut(s) 141, 346, 1684, 1933, 2813, 2947
XapI RAATTY 7 cut(s) 18, 1960, 2701, 3175, 3504, 3532, 4018
XceI RCATGY 8 cut(s) 193, 206, 238, 1510, 3193, 3604, 4699, 5456
XcmI CCANNNNNNNNNTGG 1 cut(s) 4001
XhoI CTCGAG 2 cut(s) 317, 587
XmaI CCCGGG 1 cut(s) 4662
XmiI GTMKAC 4 cut(s) 270, 823, 3380, 5348
XmnI GAANNNNTTC 1 cut(s) 767
XspI CTAG 3 cut(s) 1971, 4548, 5424
ZrmI AGTACT 1 cut(s) 4601
Zsp2I ATGCAT 3 cut(s) 1023, 2386, 4311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.