MD09G1102200.v1.1

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000798
Physical Location & Seq
Reverse (-)
7479903 .. 7481584
1682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1102200.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGAACAAGATCATGAAGTCAAATCCAGCTCTGTATGTCTTGAGGGAGCGGATAAGGAAAGGGTTGCAGTTGTATTCATCCGAGCCTACAGAACCTTATTTGTCTTCCCAAAACTATGGGGAGCTGTTCGGCAGTCAACTTATTTGGTTTGTTGATGGCACAAATGTGTATCGTGTTATAGACCATAATACACATGAAGGGAATCTTGTTACAAAATCCATCAATGGGCAGAAACGACTTGGTCAGTTGGCAAAGTCGAAAACCGCAGAAGAAGTTGATGCACTAGTGAGATCTCTACCCATTGAAGAACAACCAAAGCAAATTATTGTGACTCGTAAAGGAATGTTGGACCCTTTGGAGGTTCACTTGCTGGATTTCCCTAACATGGTCATAAAAGGAAGTGAACTGCAGCTTCCTTTCCAGGCTTGCTTGAACAAAGAAAAGGCAAATATGTTACTGAAGCCCGACAGAATGGCAATTACTGAGGCAGTCACAACAAGCACTACAAATGTGCGTGGTGATCAACTTATTGTTACCACTACGAGTTGTTATGAGCAAGAAGTACATCGGTCCAAAACTGATTGGCGTGGGAGAGTAATCTTTGCTGCAAATCTATCTGAGGACGCAAATTGCTGGTTACTTGTACGTATAAGCCCGCCCGACAATTCTCAGGTTAAGGAAATTCGATGCATTGTCACGCCACCACAATGGGGGACTCACCAGCAAGTTCATCTCCCATCGGCTCTTCCTGAGAATGATTTCCTCAATGATTTGGAGCCTTTGGGATGGATGCACACGCAGCCAAATGAGCTTCCTCAGCTTTCTCCCCAGGATATCGCATCTCACGCTAAGATGCTGGAGAACACCAAGCAATGGAATGGGGAGAAGTGCATCATTTTAACCTGCAGCTTCACTCCAGGTTCCTGCTCGTTGGCTGCATATAAGCTTACTCCAGTGGGTTATGAGTGGGGACGTCGTGTTAATACAGGAGACAGCGGAAGCAATGTTAATCAGCACGGATACCTTCCAATCCATTATGAGAAAGCTCAGATGATTCTCAGCGACCGGTTTCTTGGCTTCTTCATGATTCCTGATAATGGTCCCTGGGGCTACAACTTCATGGGGGTGAAGCATACCACAAGAATGAAGTATGACATCAAGCTTGGGATGCCGAGGGAGTACTATCACCAGGACCACCGCCCGATCCATTTCTTGGAGTTCTGCAACATGGAGGACGGTGACGCAGTAGGGGGAGGTTGTGACGACCACTTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

426

Amino Acids

48.35

Weight (kDa)

6.12

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP8_domainIV PF12134 31 - 76 2.6e-14 PRP8 domain IV core
PRP8_domainIV PF12134 76 - 150 3.6e-34 PRP8 domain IV core
JAB PF01398 224 - 285 1.7e-06 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 298 - 412 2.7e-42 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 976
Acc36I ACCTGC 1 cut(s) 911
AccB7I CCANNNNNTGG 2 cut(s) 873, 1213
AccBSI CCGCTC 1 cut(s) 49
AciI CCGC 5 cut(s) 49, 264, 656, 996, 1198
AclWI GGATC 1 cut(s) 1198
AcsI RAATTY 1 cut(s) 681
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 973
AfaI GTAC 3 cut(s) 564, 645, 1181
AfiI CCNNNNNNNGG 7 cut(s) 225, 358, 385, 710, 873, 1097, 1213
AgeI ACCGGT 1 cut(s) 1065
AgsI TTSAA 2 cut(s) 305, 433
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 5 cut(s) 420, 828, 916, 1103, 1188
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 9 cut(s) 29, 124, 412, 811, 820, 909, 946, 1046, 1162
AluI AGCT 9 cut(s) 29, 124, 412, 811, 820, 909, 946, 1046, 1162
Alw26I GTCTC 1 cut(s) 984
AlwI GGATC 1 cut(s) 1198
ApeKI GCWGC 5 cut(s) 409, 605, 799, 906, 935
ApoI RAATTY 1 cut(s) 681
ArsI GACNNNNNNTTYG 1 cut(s) 1253
AsiGI ACCGGT 1 cut(s) 1065
Asp700I GAANNNNTTC 1 cut(s) 758
AspS9I GGNCC 4 cut(s) 349, 570, 1100, 1192
AsuHPI GGTGA 5 cut(s) 530, 710, 1138, 1178, 1250
AvaII GGWCC 4 cut(s) 349, 570, 1100, 1192
BbsI GAAGAC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 5 cut(s) 421, 592, 811, 918, 922
BccI CCATC 4 cut(s) 149, 227, 745, 780
BciT130I CCWGG 5 cut(s) 422, 830, 918, 1105, 1190
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 984
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 3 cut(s) 87, 407, 904
BfuAI ACCTGC 1 cut(s) 911
BfuI GTATCC 1 cut(s) 1013
BglII AGATCT 1 cut(s) 290
BisI GCNGC 5 cut(s) 410, 606, 800, 907, 936
BlsI GCNGC 5 cut(s) 411, 607, 801, 908, 937
BmcAI AGTACT 1 cut(s) 1181
Bme1390I CCNGG 5 cut(s) 422, 830, 918, 1105, 1190
Bme18I GGWCC 4 cut(s) 349, 570, 1100, 1192
BmgT120I GGNCC 4 cut(s) 349, 570, 1100, 1192
BmiI GGNNCC 4 cut(s) 351, 777, 922, 1102
BmrFI CCNGG 5 cut(s) 422, 830, 918, 1105, 1190
BmsI GCATC 7 cut(s) 268, 677, 780, 843, 848, 900, 1158
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 3 cut(s) 878, 900, 936
Bpu10I CCTNAGC 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 61
BsaAI YACGTR 1 cut(s) 647
BsaHI GRCGYC 1 cut(s) 973
BsaJI CCNNGG 4 cut(s) 828, 1103, 1104, 1172
BsaWI WCCGGW 1 cut(s) 1065
Bsc4I CCNNNNNNNGG 7 cut(s) 225, 358, 385, 710, 873, 1097, 1213
Bse118I RCCGGY 1 cut(s) 1065
Bse1I ACTGG 1 cut(s) 953
Bse3DI GCAATG 2 cut(s) 878, 1009
BseBI CCWGG 5 cut(s) 422, 830, 918, 1105, 1190
BseDI CCNNGG 4 cut(s) 828, 1103, 1104, 1172
BseGI GGATG 4 cut(s) 77, 791, 795, 1173
BseLI CCNNNNNNNGG 7 cut(s) 225, 358, 385, 710, 873, 1097, 1213
BseMI GCAATG 2 cut(s) 878, 1009
BseMII CTCAG 7 cut(s) 474, 609, 683, 741, 830, 1061, 1072
BseNI ACTGG 1 cut(s) 953
BseXI GCAGC 5 cut(s) 421, 592, 811, 918, 922
Bsh1285I CGRYCG 1 cut(s) 1066
BshTI ACCGGT 1 cut(s) 1065
BsiEI CGRYCG 1 cut(s) 1066
BsiSI CCGG 1 cut(s) 1066
BslFI GGGAC 3 cut(s) 727, 984, 1086
BslI CCNNNNNNNGG 7 cut(s) 225, 358, 385, 710, 873, 1097, 1213
BsmAI GTCTC 1 cut(s) 984
BsmFI GGGAC 3 cut(s) 727, 984, 1086
Bsp143I GATC 4 cut(s) 9, 290, 520, 1203
BspACI CCGC 5 cut(s) 49, 264, 656, 996, 1198
BspCNI CTCAG 7 cut(s) 475, 610, 682, 742, 829, 1060, 1071
BspHI TCATGA 2 cut(s) 12, 1083
BspLI GGNNCC 4 cut(s) 351, 777, 922, 1102
BspMAI CTGCAG 2 cut(s) 411, 908
BspMI ACCTGC 1 cut(s) 911
BspPI GGATC 1 cut(s) 1198
BspQI GCTCTTC 1 cut(s) 750
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 878, 1009
BsrFI RCCGGY 1 cut(s) 1065
BsrI ACTGG 1 cut(s) 953
BssAI RCCGGY 1 cut(s) 1065
BssECI CCNNGG 4 cut(s) 828, 1103, 1104, 1172
BssMI GATC 4 cut(s) 9, 290, 520, 1203
BssNI GRCGYC 1 cut(s) 973
Bst2UI CCWGG 5 cut(s) 422, 830, 918, 1105, 1190
Bst4CI ACNGT 1 cut(s) 1238
Bst6I CTCTTC 1 cut(s) 750
BstACI GRCGYC 1 cut(s) 973
BstBAI YACGTR 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 427, 656
BstDEI CTNAG 8 cut(s) 483, 618, 669, 750, 816, 849, 1047, 1058
BstF5I GGATG 4 cut(s) 77, 791, 795, 1173
BstKTI GATC 4 cut(s) 12, 293, 523, 1206
BstMAI GTCTC 1 cut(s) 984
BstMBI GATC 4 cut(s) 9, 290, 520, 1203
BstMCI CGRYCG 1 cut(s) 1066
BstMWI GCNNNNNNNGC 5 cut(s) 799, 808, 817, 845, 1168
BstNI CCWGG 5 cut(s) 422, 830, 918, 1105, 1190
BstSCI CCNGG 5 cut(s) 420, 828, 916, 1103, 1188
BstSFI CTRYAG 3 cut(s) 87, 407, 904
BstSNI TACGTA 1 cut(s) 647
BstV1I GCAGC 5 cut(s) 421, 592, 811, 918, 922
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 2 cut(s) 116, 708
BstYI RGATCY 1 cut(s) 290
BsuI GTATCC 1 cut(s) 1013
BtsCI GGATG 4 cut(s) 77, 791, 795, 1173
BtsIMutI CAGTG 1 cut(s) 960
BveI ACCTGC 1 cut(s) 911
Cac8I GCNNGC 2 cut(s) 427, 656
CciI TCATGA 2 cut(s) 12, 1083
Cfr10I RCCGGY 1 cut(s) 1065
Cfr13I GGNCC 4 cut(s) 349, 570, 1100, 1192
CseI GACGC 2 cut(s) 632, 1250
Csp6I GTAC 3 cut(s) 563, 644, 1180
CspAI ACCGGT 1 cut(s) 1065
CviAII CATG 6 cut(s) 13, 194, 385, 1084, 1120, 1228
CviQI GTAC 3 cut(s) 563, 644, 1180
DdeI CTNAG 8 cut(s) 483, 618, 669, 750, 816, 849, 1047, 1058
DpnI GATC 4 cut(s) 11, 292, 522, 1205
DpnII GATC 4 cut(s) 9, 290, 520, 1203
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
Eco105I TACGTA 1 cut(s) 647
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 4 cut(s) 349, 570, 1100, 1192
Eco57I CTGAAG 1 cut(s) 479
EcoRII CCWGG 5 cut(s) 420, 828, 916, 1103, 1188
EcoRV GATATC 1 cut(s) 835
EcoT22I ATGCAT 1 cut(s) 692
FaeI CATG 6 cut(s) 16, 197, 388, 1087, 1123, 1231
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 3 cut(s) 727, 984, 1086
FatI CATG 6 cut(s) 12, 193, 384, 1083, 1119, 1227
FauI CCCGC 1 cut(s) 663
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 5 cut(s) 410, 606, 800, 907, 936
FokI GGATG 4 cut(s) 64, 798, 802, 1180
Fsp4HI GCNGC 5 cut(s) 410, 606, 800, 907, 936
FspBI CTAG 1 cut(s) 284
GluI GCNGC 5 cut(s) 410, 606, 800, 907, 936
GsuI CTGGAG 3 cut(s) 878, 900, 936
HapII CCGG 1 cut(s) 1066
HgaI GACGC 2 cut(s) 632, 1250
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 6 cut(s) 16, 197, 388, 1087, 1123, 1231
HincII GTYRAC 1 cut(s) 137
HindII GTYRAC 1 cut(s) 137
HindIII AAGCTT 2 cut(s) 944, 1160
HinfI GANTC 5 cut(s) 202, 331, 715, 1054, 1087
HpaII CCGG 1 cut(s) 1066
HphI GGTGA 5 cut(s) 530, 710, 1138, 1178, 1250
Hpy166II GTNNAC 3 cut(s) 137, 364, 404
Hpy188I TCNGA 3 cut(s) 82, 619, 1050
Hpy188III TCNNGA 5 cut(s) 13, 40, 749, 1084, 1091
Hpy8I GTNNAC 3 cut(s) 137, 364, 404
Hpy99I CGWCG 1 cut(s) 978
HpyAV CCTTC 2 cut(s) 191, 1034
HpyCH4III ACNGT 1 cut(s) 1238
HpyCH4IV ACGT 2 cut(s) 646, 973
HpyF10VI GCNNNNNNNGC 5 cut(s) 799, 808, 817, 845, 1168
HpyF3I CTNAG 8 cut(s) 483, 618, 669, 750, 816, 849, 1047, 1058
HpySE526I ACGT 2 cut(s) 646, 973
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 6 cut(s) 16, 197, 388, 1087, 1123, 1231
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 4 cut(s) 9, 290, 520, 1203
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 3 cut(s) 46, 121, 775
Lsp1109I GCAGC 5 cut(s) 421, 592, 811, 918, 922
LweI GCATC 7 cut(s) 268, 677, 780, 843, 848, 900, 1158
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 646, 973
MaeIII GTNAC 9 cut(s) 208, 328, 453, 490, 532, 636, 694, 1238, 1259
MalI GATC 4 cut(s) 11, 292, 522, 1205
MbiI CCGCTC 1 cut(s) 49
MboI GATC 4 cut(s) 9, 290, 520, 1203
MboII GAAGA 5 cut(s) 96, 281, 317, 737, 1072
MflI RGATCY 1 cut(s) 290
MluCI AATT 5 cut(s) 321, 477, 628, 664, 681
MlyI GAGTC 2 cut(s) 325, 709
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 9 cut(s) 36, 352, 478, 613, 773, 825, 1167, 1225, 1247
Mph1103I ATGCAT 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 758
MseI TTAA 4 cut(s) 675, 899, 981, 1008
MslI CAYNNNNRTG 4 cut(s) 164, 706, 1124, 1142
MspA1I CMGCKG 1 cut(s) 996
MspI CCGG 1 cut(s) 1066
MspR9I CCNGG 5 cut(s) 422, 830, 918, 1105, 1190
MvaI CCWGG 5 cut(s) 422, 830, 918, 1105, 1190
MwoI GCNNNNNNNGC 5 cut(s) 799, 808, 817, 845, 1168
NdeII GATC 4 cut(s) 9, 290, 520, 1203
NlaIII CATG 6 cut(s) 16, 197, 388, 1087, 1123, 1231
NlaIV GGNNCC 4 cut(s) 351, 777, 922, 1102
NmeAIII GCCGAG 1 cut(s) 1197
NmuCI GTSAC 5 cut(s) 328, 490, 694, 1238, 1259
NsiI ATGCAT 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 164
PagI TCATGA 2 cut(s) 12, 1083
PasI CCCWGGG 1 cut(s) 1104
PciSI GCTCTTC 1 cut(s) 750
PdmI GAANNNNTTC 1 cut(s) 758
PfeI GAWTC 3 cut(s) 202, 1054, 1087
PflFI GACNNNGTC 1 cut(s) 240
PflMI CCANNNNNTGG 2 cut(s) 873, 1213
PinAI ACCGGT 1 cut(s) 1065
PkrI GCNGC 5 cut(s) 411, 607, 801, 908, 937
PleI GAGTC 2 cut(s) 325, 709
PpsI GAGTC 2 cut(s) 325, 709
Ppu21I YACGTR 1 cut(s) 647
Psp6I CCWGG 5 cut(s) 420, 828, 916, 1103, 1188
PspGI CCWGG 5 cut(s) 420, 828, 916, 1103, 1188
PspN4I GGNNCC 4 cut(s) 351, 777, 922, 1102
PspPI GGNCC 4 cut(s) 349, 570, 1100, 1192
PstI CTGCAG 2 cut(s) 411, 908
PsuI RGATCY 1 cut(s) 290
PsyI GACNNNGTC 1 cut(s) 240
RsaI GTAC 3 cut(s) 564, 645, 1181
RsaNI GTAC 3 cut(s) 563, 644, 1180
RseI CAYNNNNRTG 4 cut(s) 164, 706, 1124, 1142
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 4 cut(s) 675, 899, 981, 1008
SatI GCNGC 5 cut(s) 410, 606, 800, 907, 936
Sau3AI GATC 4 cut(s) 9, 290, 520, 1203
Sau96I GGNCC 4 cut(s) 349, 570, 1100, 1192
ScaI AGTACT 1 cut(s) 1181
SchI GAGTC 2 cut(s) 325, 709
ScrFI CCNGG 5 cut(s) 422, 830, 918, 1105, 1190
SfaNI GCATC 7 cut(s) 268, 677, 780, 843, 848, 900, 1158
SfcI CTRYAG 3 cut(s) 87, 407, 904
SinI GGWCC 4 cut(s) 349, 570, 1100, 1192
SmiMI CAYNNNNRTG 4 cut(s) 164, 706, 1124, 1142
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
SnaBI TACGTA 1 cut(s) 647
SpeI ACTAGT 1 cut(s) 283
Sse9I AATT 5 cut(s) 321, 477, 628, 664, 681
SsiI CCGC 5 cut(s) 49, 264, 656, 996, 1198
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 5 cut(s) 420, 828, 916, 1103, 1188
TaaI ACNGT 1 cut(s) 1238
TaiI ACGT 2 cut(s) 649, 976
TaqI TCGA 2 cut(s) 257, 685
TaqII GACCGA 1 cut(s) 558
TasI AATT 5 cut(s) 321, 477, 628, 664, 681
TatI WGTACW 2 cut(s) 562, 1179
TfiI GAWTC 3 cut(s) 202, 1054, 1087
Tru1I TTAA 4 cut(s) 675, 899, 981, 1008
Tru9I TTAA 4 cut(s) 675, 899, 981, 1008
TscAI CASTG 1 cut(s) 960
TseFI GTSAC 5 cut(s) 328, 490, 694, 1238, 1259
TseI GCWGC 5 cut(s) 409, 605, 799, 906, 935
Tsp45I GTSAC 5 cut(s) 328, 490, 694, 1238, 1259
TspDTI ATGAA 8 cut(s) 17, 29, 66, 210, 719, 1072, 1108, 1160
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 1 cut(s) 960
Tth111I GACNNNGTC 1 cut(s) 240
Van91I CCANNNNNTGG 2 cut(s) 873, 1213
VpaK11BI GGWCC 4 cut(s) 349, 570, 1100, 1192
XapI RAATTY 1 cut(s) 681
XmnI GAANNNNTTC 1 cut(s) 758
XspI CTAG 1 cut(s) 284
ZraI GACGTC 1 cut(s) 974
ZrmI AGTACT 1 cut(s) 1181
Zsp2I ATGCAT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.