Rroxscaffold_2G00121310

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000798
Physical Location & Seq
Reverse (-)
53878570 .. 53880561
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121310.1

Sequence Viewer

Length: 768 bp
ATGGCGAACCCGTGGGTGAAGTTTGGGAAGGTTTCGTGGCCGGTAGCTCGAGTTATCAATCTTGGAACAAAAACAGGTTTTTCGGCCTCCGCCGTCGTCTCCGGTGGTTCGCAGCTCAAGGCGCCTCCCTACAGCTGCGCACGATCGTCTATGGAACAATACCTGTCATCCCAAAACTATGGAGAGATTTTCAGCCATCAGGTTATTTGGTTTGTCGACAATAGACTTGAAGAAAATCTTACAATGGAACCCATTGGTGCAGTTATCATATTAAATCCACGGACTGGGCAGCTGTTTCTGAAGGTCAACCCAAGTACAGAAACTGCAGAAGAGGTTGCTGCTCTGGTGCAGTCTTTACCAGCTGGAGAATGTCCGAAACAAATTATTGTGGAGGTGAGTAAGGAATTCTTGATACACTACAGGCTGCTGAGCCACGTACGGATGGTTCCATTTAACGTCTATGATGATTGGTCGAGTAGCATTTCACCATACGCTGCATTTTCCCGGCTCATTCTGATTCTACGTGCCCTTCATGTGAACGATGAGAAGGCAAAGATGTTATTAAATCCTGACACAACAGTAGTTACTGAGCCCAATCACATCTGGCCTTCTCTTTCTGAATTTCAATGGAGGAAGGTCGAGGTTGCTCTGAGGGATCTCATTCTGTCAGATTATGCAGAAAAAGAACAATGTGATCACATCCGCCCTGACCCCAAGTGGAGATTCGTGATATTATTCTTGGAGCGTATTGAGATGATCTCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

255

Amino Acids

29.0

Weight (kDa)

6.09

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP8_domainIV PF12134 52 - 132 1.5e-14 PRP8 domain IV core
PRP8_domainIV PF12134 146 - 223 4.4e-31 PRP8 domain IV core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 121
AccB7I CCANNNNNTGG 1 cut(s) 284
AccI GTMKAC 1 cut(s) 216
AciI CCGC 2 cut(s) 90, 703
AclWI GGATC 1 cut(s) 663
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 404, 620
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 316, 438
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 2 cut(s) 230, 626
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AluBI AGCT 5 cut(s) 47, 115, 135, 292, 362
AluI AGCT 5 cut(s) 47, 115, 135, 292, 362
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 1 cut(s) 663
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 48
AoxI GGCC 3 cut(s) 38, 84, 605
ApeKI GCWGC 6 cut(s) 112, 135, 289, 338, 424, 494
ApoI RAATTY 2 cut(s) 404, 620
AspLEI GCGC 2 cut(s) 124, 140
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 3 cut(s) 28, 406, 477
AvaI CYCGRG 1 cut(s) 48
BaeGI GKGCMC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 594
BbvI GCAGC 6 cut(s) 122, 124, 301, 325, 411, 481
BccI CCATC 2 cut(s) 204, 436
BceAI ACGGC 1 cut(s) 77
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 103
BfmI CTRYAG 3 cut(s) 130, 324, 418
BfoI RGCGCY 1 cut(s) 125
BisI GCNGC 6 cut(s) 113, 136, 290, 339, 425, 495
BlpI GCTNAGC 1 cut(s) 428
BlsI GCNGC 6 cut(s) 114, 137, 291, 340, 426, 496
Bme1390I CCNGG 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 48
BmiI GGNNCC 3 cut(s) 123, 249, 447
BmrFI CCNGG 1 cut(s) 505
BmrI ACTGGG 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 294
BplI GAGNNNNNCTC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 505
BsaAI YACGTR 2 cut(s) 436, 524
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 11, 278
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 11, 278
BseGI GGATG 3 cut(s) 167, 447, 699
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 3 cut(s) 419, 579, 641
BseNI ACTGG 1 cut(s) 289
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 6 cut(s) 122, 124, 301, 325, 411, 481
BsgI GTGCAG 2 cut(s) 279, 368
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 3 cut(s) 40, 86, 607
BshNI GGYRCC 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 3 cut(s) 41, 102, 505
BsiWI CGTACG 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsnI GGCC 3 cut(s) 40, 86, 607
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 2 cut(s) 529, 594
Bsp143I GATC 4 cut(s) 143, 655, 694, 756
Bsp1720I GCTNAGC 1 cut(s) 428
BspACI CCGC 2 cut(s) 90, 703
BspANI GGCC 3 cut(s) 40, 86, 607
BspCNI CTCAG 3 cut(s) 420, 580, 642
BspLI GGNNCC 3 cut(s) 123, 249, 447
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 1 cut(s) 289
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 11, 278
BssMI GATC 4 cut(s) 143, 655, 694, 756
BssNI GRCGYC 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 580
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 122
BstBAI YACGTR 2 cut(s) 436, 524
BstDEI CTNAG 3 cut(s) 428, 588, 650
BstDSI CCRYGG 2 cut(s) 11, 278
BstF5I GGATG 3 cut(s) 167, 447, 699
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 140
BstKTI GATC 4 cut(s) 146, 658, 697, 759
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 4 cut(s) 143, 655, 694, 756
BstMCI CGRYCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 503
BstSFI CTRYAG 3 cut(s) 130, 324, 418
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 6 cut(s) 122, 124, 301, 325, 411, 481
BstX2I RGATCY 1 cut(s) 655
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 1 cut(s) 655
BsuRI GGCC 3 cut(s) 40, 86, 607
BtgI CCRYGG 2 cut(s) 11, 278
BtsCI GGATG 3 cut(s) 167, 447, 699
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 2 cut(s) 124, 140
Cfr10I RCCGGY 1 cut(s) 40
Csp6I GTAC 2 cut(s) 315, 437
CviAII CATG 1 cut(s) 533
CviQI GTAC 2 cut(s) 315, 437
DdeI CTNAG 3 cut(s) 428, 588, 650
DinI GGCGCC 1 cut(s) 123
DpnI GATC 4 cut(s) 145, 657, 696, 758
DpnII GATC 4 cut(s) 143, 655, 694, 756
EaeI YGGCCR 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EciI GGCGGA 2 cut(s) 79, 692
Eco24I GRGCYC 1 cut(s) 594
Eco57I CTGAAG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 594
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 1 cut(s) 536
FaiI YATR 7 cut(s) 152, 180, 269, 462, 490, 534, 675
FalI AAGNNNNNCTT 4 cut(s) 222, 254, 392, 424
FatI CATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 694
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 6 cut(s) 113, 136, 290, 339, 425, 495
FokI GGATG 3 cut(s) 154, 454, 686
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 6 cut(s) 113, 136, 290, 339, 425, 495
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 123, 139
GluI GCNGC 6 cut(s) 113, 136, 290, 339, 425, 495
GsuI CTGGAG 1 cut(s) 384
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 40, 86, 607
HapII CCGG 3 cut(s) 41, 102, 505
HhaI GCGC 2 cut(s) 124, 140
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 1 cut(s) 536
Hin6I GCGC 2 cut(s) 122, 138
HinP1I GCGC 2 cut(s) 122, 138
HincII GTYRAC 2 cut(s) 217, 307
HindII GTYRAC 2 cut(s) 217, 307
HinfI GANTC 2 cut(s) 517, 723
HpaII CCGG 3 cut(s) 41, 102, 505
HphI GGTGA 3 cut(s) 28, 406, 477
Hpy166II GTNNAC 3 cut(s) 217, 307, 538
Hpy188I TCNGA 6 cut(s) 300, 375, 516, 619, 651, 670
Hpy188III TCNNGA 3 cut(s) 409, 569, 727
Hpy8I GTNNAC 3 cut(s) 217, 307, 538
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 6 cut(s) 22, 295, 539, 541, 618, 628
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4IV ACGT 3 cut(s) 435, 456, 523
HpyCH4V TGCA 5 cut(s) 260, 326, 349, 497, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 3 cut(s) 428, 588, 650
HpySE526I ACGT 3 cut(s) 435, 456, 523
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 1 cut(s) 536
HspAI GCGC 2 cut(s) 122, 138
KasI GGCGCC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 4 cut(s) 143, 655, 694, 756
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 6 cut(s) 122, 124, 301, 325, 411, 481
MaeII ACGT 3 cut(s) 435, 456, 523
MaeIII GTNAC 1 cut(s) 583
MalI GATC 4 cut(s) 145, 657, 696, 758
MboI GATC 4 cut(s) 143, 655, 694, 756
MboII GAAGA 2 cut(s) 242, 341
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 2 cut(s) 529, 594
MluCI AATT 3 cut(s) 381, 404, 620
Mly113I GGCGCC 1 cut(s) 122
MnlI CCTC 7 cut(s) 97, 135, 325, 385, 624, 634, 645
MseI TTAA 4 cut(s) 272, 453, 563, 766
MspA1I CMGCKG 3 cut(s) 135, 292, 362
MspI CCGG 3 cut(s) 41, 102, 505
MspR9I CCNGG 1 cut(s) 505
MwoI GCNNNNNNNGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 122
NciI CCSGG 1 cut(s) 505
NdeII GATC 4 cut(s) 143, 655, 694, 756
NlaIII CATG 1 cut(s) 536
NlaIV GGNNCC 3 cut(s) 123, 249, 447
NsbI TGCGCA 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 48
PfeI GAWTC 2 cut(s) 517, 723
Pfl23II CGTACG 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 284
PkrI GCNGC 6 cut(s) 114, 137, 291, 340, 426, 496
Ple19I CGATCG 1 cut(s) 146
PluTI GGCGCC 1 cut(s) 125
Ppu21I YACGTR 2 cut(s) 436, 524
PspLI CGTACG 1 cut(s) 436
PspN4I GGNNCC 3 cut(s) 123, 249, 447
PspXI VCTCGAGB 1 cut(s) 48
PsrI GAACNNNNNNTAC 2 cut(s) 429, 461
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 655
PvuI CGATCG 1 cut(s) 146
PvuII CAGCTG 3 cut(s) 135, 292, 362
RsaI GTAC 2 cut(s) 316, 438
RsaNI GTAC 2 cut(s) 315, 437
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 4 cut(s) 272, 453, 563, 766
SatI GCNGC 6 cut(s) 113, 136, 290, 339, 425, 495
Sau3AI GATC 4 cut(s) 143, 655, 694, 756
ScrFI CCNGG 1 cut(s) 505
SduI GDGCHC 2 cut(s) 529, 594
SfcI CTRYAG 3 cut(s) 130, 324, 418
SfoI GGCGCC 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 48
SlaI CTCGAG 1 cut(s) 48
SmlI CTYRAG 2 cut(s) 48, 116
SmoI CTYRAG 2 cut(s) 48, 116
Sse9I AATT 3 cut(s) 381, 404, 620
SsiI CCGC 2 cut(s) 90, 703
SspDI GGCGCC 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 503
TaaI ACNGT 1 cut(s) 580
TaiI ACGT 3 cut(s) 438, 459, 526
TaqI TCGA 4 cut(s) 49, 216, 473, 639
TasI AATT 3 cut(s) 381, 404, 620
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 2 cut(s) 517, 723
Tru1I TTAA 4 cut(s) 272, 453, 563, 766
Tru9I TTAA 4 cut(s) 272, 453, 563, 766
TseI GCWGC 6 cut(s) 112, 135, 289, 338, 424, 494
TspDTI ATGAA 1 cut(s) 521
TspGWI ACGGA 2 cut(s) 295, 454
Van91I CCANNNNNTGG 1 cut(s) 284
XapI RAATTY 2 cut(s) 404, 620
XhoI CTCGAG 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.