FvH4_6g29610

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000798
Physical Location & Seq
Reverse (-)
22848662 .. 22858643
9982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29610.t1

Sequence Viewer

Length: 7038 bp
ATGTGGAACAACGGCCAGATTGTGCCGCCTGGCACCGGTGGCTCGTCGATTCCGCCACCGCCTGCTGCTCAGCCCTCTTATACGGTGCTGCCGCCTCCGGCAGATGCCGAGGCCGTGCTCGAAGAGAAGGCTCGTAAATGGCAGCAGCTCAATTCCAAGCGCTACAGCGACAAGCGCAAGTTTGGCTTCGTTGAGACGCAGAAGGAGGACATGCCTCCGGAGCATGTCAGGAAGATTATTAGAGACCATGGGGACATGTCCTCGAAGAAATTCCGTCATGATAAACGTGTGTATCTAGGAGCACTTAAATTTGTTCCGCACGCAGTTTACAAGCTTCTCGAGAATATGCCAATGCCCTGGGAGCAGGTCCGTGATGTGAAGGTTCTGTACCATATTACTGGGGCAATTACATTTGTGAATGAGATACCTTGGGTTGTTGAACCTATATATTTGGCTCAGTGGGGTTCCATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGTCGACATTTTAAAAGAATGCGGTTTCCACCTTTTGATGATGAGGAACCTCCATTAGATTATGCTGATAATCTGTTAGATGTTGATCCTCTAGAGCCAATTCAACTGGAGTTGGATGAAGAAGAAGATTCTGCTGTTTATACTTGGTTTTATGACCATAAACCTCTAGTCAAGACAAAACTCATTAATGGTCCAAGTTATCGGAAATGGCATCTGTCACTTCCCATTATGGCAACTCTCCACCGGCTTGCAGGACAGCTGCTTTCTGATTTGATTGACCGCAATTATTTCTACTTGTTTGACATGGAGTCATTCTTTACAGCAAAAGCACTGAATATGTGCATTCCAGGTGGGCCTAAGTTTGAACCGCTGTATCGGGATATGGAGAAAGGGGATGAGGACTGGAATGAGTTTAATGACATTAACAAGCTGATTATTCGGTCACCTCTCAGAACAGAGTACAGAATTGCATTCCCTCATCTCTACAATAATAGACCTAGGAAGGTAAAGCTCTGCGTGTATCACACACCCATGGTAATGTACATAAAAACTGAAGATCCTGATCTACCTGCATTCTATTATGATCCCTTGATACACCCAATCCCCAGCACAAACAAGGAGCGACGTGAAAAGAAAGTTGATGAGGATGATGACACTTTCATATTGCCAGAAGGAGTAGAGCCATTTCTGAGTGATACTCAGCTCTATACTGACACAACAGCAGCTGGAGTTTCCCTCTTGTTTGCACCACGCCCCTTTAACATGAGATCTGGCCGGACTCGCCGTGCAGAAGATATACCCCTTGTGTCAGAGTGGTATAAAGAGCATTGTCCTCCTTCCTATCCAGTTAAGGTTCGGGTCAGCTATCAGAAGTTGTTAAAATGTTTTGTGTTGAACGAGTTGCATCATAGGCCACCCAAGGCTCAGAAGAAGAAGCACTTGTTTCGATCACTCCAAGCTACTAAGTTCTTCCAAACTACCGAACTTGATTGGGCTGAAGCGGGTCTTCAAGTCTGCAAGCAGGGTTATAACATGCTAAATCTCCTAATCCATAGAAAAAATTTGAATTACCTCCATCTTGATTACAATTTCAACCTGAAGCCTGTGAAAACCTTGACAACCAAAGAGCGTAAGAAGTCCCGTTTTGGAAATGCTTTTCATCTTTGCCGTGAGATCTTGCGGTTGACAAAGCTGGTAGTTGATGCCAACATTCAGTTCCGTTTGGGTAATGTTGATGCCTTTCAGTTGGCTGATGGCCTGCAGTACACTTTCTCTCATGTTGGTCAGTTGACTGGTATGTATCGTTACAAATACAGGTTGATGCGTCAGATTAGAATGTGCAAAGATTTGAAGCACTTGATTTACTACCGCTTCAACACGGGGCCAGTTGGGAAAGGACCTGGATGTGGATTCTGGGCACCAATGTGGAGGGTCTGGTTGTTCTTCCTTCGTGGCATAGTTCCTCTTTTGGAAAGGTGGTTGGGAAATTTACTTGCTCGGCAATTTGAAGGGCGCCATTCAAAAGGAGTGGCAAAGACTGTAACTAAGCAGCGTGTGGAGAGCCATTTTGACTTGGAGCTTCGAGCTGCTGTCATGCATGATGTTCTTGATGCGATGCCTGAGGGAATCAAGCAAAATAAGGCCAGAACTATATTGCAACATCTCAGTGAGGCATGGCGTTGCTGGAAAGCAAATATTCCTTGGAAGGTACCTGGTTTGCCAGTTCCCATTGAGAATATGATTCTTCGCTACGTCAAGTCAAAGGCAGACTGGTGGACAAATGTGGCTCATTACAATCGTGAACGTATAAGAAGAGGTGCTACTGTAGATAAGACCGTTTGTCGAAAGAATCTCGGAAGATTAACTCGCCTATGGCTGAAGGCTGAGCAGGAACGTCAACACAACTATTTGAAAGATGGTCCATATGTTACCCCAGAGGAGGCAGTTGCAATATACACGACAACTGTGCATTGGTTGGAGTCAAGAAAGTTCAGCCCCATCCCATTTCCACCTTTGTCGTACAAGCATGATACCAAGCTACTTATCCTTGCTCTGGAGAGGTTGAAGGAATCATATAGTGTTGCTGTGAGATTGAATCAGCTTCAAAGAGAAGAGCTTGGTCTTATTGAACAAGCGTATGATAACCCGCATGAGGCATTGTCTCGAATTAAGCGTCACTTACTCACTCAGCGTGCCTTCAAAGAAGTTGGCATAGAGTTCATGGATTTGTACAGCTATCTCATTCCAGTTTATGAGATTGAACCTCTTGAGAAGATTACAGATGCGTATCTTGATCAATATCTGTGGTATGAGGGTGACAAACGTCATCTCTTCCCGAACTGGATCAAGCCTGCAGATTCGGAGCCGCCTCCTCTCTTGGTTTATAAATGGTGTCAGGGAATAAACAATTTACAGAGCATATGGGACACTGGTGACGGGCAGTGTGTTGTCATGCTTCAGACCAAGTTTGAGAAGTTCTTTGATAAGATTGACTTGACGATGCTAAATAGGCTTCTACGTCTAGTCCTTGATCATAATATCGCTGATTATGTCACCGCGAAGAACAATGTGGTATTGTCCTACAAGGATATGAGTCATACAAATTCTTATGGTCTTATTCGCGGTCTTCAGTTTGCTTCCTTCGTTGTACAATATTACGGGCTTGTGCTGGATCTGTTGCTTCTTGGATTGACTCGAGCCAGTGAGATTGCTGGTCCACCCCAGATGCCGAATGAGTTCATCACTTATTGGGACACAAAGGTGGAGACCCGACATCCTATCAGGCTGTACTCTCGGTACATAGACAAGGTGCACATCTTGTTTCGCTTCACTCATGATGAAGCCCGGGATCTAATCCAGCGATATCTCACTGAACATCCAGATCCTAATAATGAGAATATGGTGGGATATAATAACAAGAAGTGCTGGCCAAGAGATGCTAGAATGAGGCTCATGAAGCATGATGTCAATCTTGGGAGAAGTGTTTTCTGGGATATGAAGAACCGTCTTCCTCGAAGCATCACAACTTTAGAGTGGGAGAACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTCTTCAGCATGTGTGGATTTGAGGTTCGCATACTTCCCAAGATAAGAATGAGCCAAGAGGCATTCAGCAACACAAGAGATGGAGTCTGGAATTTGCAGAATGAACAGACAAAGGAAAGGACTGCAGTTGCTTTCTTACGGGTTGATGATGAACACATGAAGATATTTGAGAATCGTGTGAGGCAGATCCTTATGTCGTCAGGGTCTACCACATTTACAAAAATTGTCAACAAATGGAATACTGCTCTGATTGGTCTTATGACTTACTTCCGTGAAGCGACTGTACATACACAAGAGCTGTTAGATTTACTAGTCAAGTGCGAGAACAAAATTCAGACCCGTATTAAGATTGGATTGAATTCCAAGATGCCTAGTAGGTTTCCACCTGTCATATTTTACACACCAAAAGAAATTGGAGGACTTGGTATGTTATCCATGGGTCACATATTGATTCCCCAAAGTGATCTCAGATACAGTCAGCAGACTGATGTTGGTGTGACACACTTTAGGAGTGGTATGAGTCATGAAGAGGACCAGCTGATTCCAAATCTGTACCGCTACATACAACCTTGGGAGAGTGAATTCATTGATTCCCAGCGTGTTTGGGCAGAATATGCACTTAAAAGGCAGGAGGCTCAAGCACAAAATAGGCGTCTGACCCTTGAAGATTTGGAAGATTCGTGGGATAGGGGAATTCCTCGCATAAATACTTTGTTCCAGAAGGACCGGCACACACTAGCATATGACAAAGGGTGGAGGGTTCGTACTGATTTTAAGCAGTACCAGGTCTTGAAACAGAATCCTTTTTGGTGGACTCACCAAAGGCATGACGGGAAGCTTTGGAACTTGAATAACTACAGAACTGATGTTATTCAAGCTCTTGGAGGTGTAGAGGGAATCTTGGAACACACCTTATTTAAAGGAACATACTTCCCTACCTGGGAGGGTCTCTTCTGGGAAAAAGCATCTGGTTTCGAGGAATCGATGAAGTACAAGAAGCTGACCAATGCACAGAGATCTGGGCTCAACCAAATTCCCAATCGTAGGTTCACACTTTGGTGGTCGCCAACCATTAATCGGGCTAATGTATATGTCGGTTTCCAAGTGCAGTTGGATCTTACTGGGATCTTTATGCATGGAAAAATCCCAACCTTGAAGATATCCTTGATTCAAATATTCCGTGCACATTTGTGGCAAAAGATCCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAGGAGTTAGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACAATTCATCCCAGGAAGAGTTACAAAATGAACAGTTCTTGTGCTGACATTCTTCTCTTTGCCGCCCATAGATGGCCAATGTCAAAACCTAGTCTTGTGGCGGAGCCAAAGGATGTCTTTGATCAGAAAGCAAGCAACAAGTACTGGATAGATGTACAACTACGATGGGGAGATTATGATTCCCACGATATTGAACGTTACACCCGAGCCAAGTTTATGGATTATACAACAGACAACATGTCTATCTATCCCTCCCCCACAGGGGTGATGATTGGTCTTGATCTGGCGTATAATTTACATTCTGCTTTTGGTAATTGGTTTCCTGGGTCCAAACCCCTGCTTCAGCAGGCAATGAACAAAATAATGAAGTCAAATCCAGCTCTGTATGTCTTGCGGGAGAGGATACGGAAAGGATTGCAGCTTTACTCTTCGGAACCTACAGAACCTTACTTATCATCCCAGAACTATGGGGAGATTTTCAGCAATCAAATTATTTGGTTTGTTGATGATACTAATGTGTATCGAGTTACCATCCACAAGACATTTGAAGGAAATCTGACTACAAAACCAATTAACGGTGCAATTTTCATATTCAATCCAAGGACTGGGCAGCTGTTCCTGAAGGTCATCCACACAAGTGTGTGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCCAAGTGGAAAACTGCAGAAGAAGTTGCTGCACTTGTGCGCTCTTTACCAGTAGAAGAGCAGCCAAAACAAATTATTGTGACTCGAAAGGGAATGCTTGATCCACTGGAGGTCCACTTGTTGGATTTCCCGAACATTGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTCCAGGCTTGTCTCAAGATTGAGAAATTTGGTGATCTGATATTGAAGGCTACTGAGCCACAGATGGTTCTTTTCAACATTTATGATGATTGGTTGAAGAGTATATCATCATATACTGCATTCTCTCGGCTGATTTTGATTCTTCGTGCACTTCATGTAAACAATGAGAAGGCAAAGATGTTACTGAAACCTGACACAACAGTCATTACTGAGCCACACCACATCTGGCCTTCTCTCTCCGATGATCAATGGATGAAGGTTGAGGTTGCTCTAAGGGATCTGATTCTCTCGGACTATGCAAAAAAGAATAACGTGAACACATCGGCTCTGACACAGTCTGAGATTCGTGATATCATTCTTGGAGCTGAGATTACTCCTCCTTCACAACAAAGGCAACAAATTGCAGAGATTGAAAAGCAGCATAAAGAAGCAAGTCAGCTGACAGCAGTTACTACTAGGACTACGAATGTGCATGGTGATGAGCTCATTGTCACCACTACTAGTCCTTATGAGCAAGCTGCATTTGGGTCCAAGACTGACTGGCGTGTGAGAGCAATATCGGCTACCAATCTCTACCTTCGTGTCAACCATATATATGTGAATTCAGAAGATGTAAAGGAAACGGGTTACACTTATATCATGCCGAAGAACATATTGAAGAAGTTCATATGCGTTGCGGATCTGAGGACACAAATTGCTGGCTACTTGTATGGTGTAAGCCCACCTGACAACCCTCAGGTCAAGGAAATTCGCTGCATTGCCATGCCACCGCAGTGGGGAACCCACCAGCAAGTCAATCTTCCAACAGCTCTTCCCGAGCATGATTTTCTCAATGATCTGGAACCTTTGGGATGGATGCACACACAACCAAACGAACTTCCTCAGCTTTCTCCTCAGGATTTAACCTCTCATGCTAAGATCTTGGAGAACACCAAGCAATGGGATGGGGAAAAGTGCATCGTCTTAACTTGCAGTTTCACTCCTGGTTCCTGCTCGTTAACTGCATACAAACTCACTCCATCTGGTTATGAGTGGGGACGGGTCAACAAAGACACAGGAAGCAATCCACACGGTTACCTCCCAACTCATTACGAGAAGGTCCAAATGCTTCTCAGTGACAGGTTCCTTGGTTTCTATATGATTCCTGATAATGGTCCATGGAACTACAACTTCATGGGTGTGAAGCATACTCAAGGCATGAAGTACGGGGTCAAGCTTGGGACACCAAGGGAGTACTATCATGAGGACCACCGGCCGACCCATTACCTTGAGTTTAGCAACATGGAGGAGGGTGACACAGTGGTGGGAGATCGTGACGATACCTTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2346

Amino Acids

273.87

Weight (kDa)

8.9

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 219 2.7e-85 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 405 - 810 1.1e-235 PROCN (NUC071) domain
RRM_4 PF10598 995 - 1085 2.4e-46 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1247 - 1382 4e-75 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1452 - 1610 8.7e-89 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1770 - 1999 2.6e-129 PRP8 domain IV core
JAB PF01398 2110 - 2209 1.1e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2222 - 2342 1.7e-48 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1530, 2886
AasI GACNNNNNNGTC 2 cut(s) 5523, 5906
Acc36I ACCTGC 2 cut(s) 355, 1078
Acc65I GGTACC 1 cut(s) 2209
AccB1I GGYRCC 4 cut(s) 32, 1918, 2013, 2209
AccB7I CCANNNNNTGG 3 cut(s) 5471, 5659, 6645
AccI GTMKAC 2 cut(s) 505, 3788
AccII CGCG 2 cut(s) 3059, 3123
AccIII TCCGGA 1 cut(s) 217
AclI AACGTT 1 cut(s) 5062
AcoI YGGCCR 6 cut(s) 13, 1273, 3428, 4940, 5538, 6959
AcuI CTGAAG 9 cut(s) 1074, 1518, 1619, 2399, 2942, 3113, 3571, 5192, 5507
AcyI GRCGYC 2 cut(s) 2014, 4234
AfeI AGCGCT 1 cut(s) 161
AflIII ACRYGT 3 cut(s) 255, 286, 5103
AgeI ACCGGT 1 cut(s) 35
AhdI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
AhlI ACTAGT 2 cut(s) 3892, 6206
AjiI CACGTC 1 cut(s) 1127
AjuI GAANNNNNNNTTGG 4 cut(s) 1700, 1732, 4613, 4645
AleI CACNNNNGTG 9 cut(s) 1924, 3260, 4639, 4771, 5129, 5502, 5504, 6478, 7007
AloI GAACNNNNNNTCC 2 cut(s) 3591, 3623
Alw21I GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5857, 6192
Alw26I GTCTC 7 cut(s) 188, 237, 2667, 3260, 4535, 4844, 5723
Alw44I GTGCAC 3 cut(s) 3311, 4764, 5853
AlwNI CAGNNNCTG 1 cut(s) 1226
Ama87I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
Aor13HI TCCGGA 1 cut(s) 217
Aor51HI AGCGCT 1 cut(s) 161
ApaLI GTGCAC 3 cut(s) 3311, 4764, 5853
ArsI GACNNNNNNTTYG 4 cut(s) 2659, 2691, 4219, 4251
AseI ATTAAT 2 cut(s) 687, 4656
AsiGI ACCGGT 1 cut(s) 35
Asp700I GAANNNNTTC 4 cut(s) 269, 3236, 4869, 6368
Asp718I GGTACC 1 cut(s) 2209
AspA2I CCTAGG 1 cut(s) 998
AspLEI GCGC 4 cut(s) 162, 177, 2016, 5580
AsuC2I CCSGG 2 cut(s) 3346, 3347
AvaI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
AvrII CCTAGG 1 cut(s) 998
AxyI CCTNAGG 3 cut(s) 2121, 6441, 6600
BaeGI GKGCMC 4 cut(s) 1921, 3315, 4768, 5857
BaeI ACNNNNGTAYC 6 cut(s) 275, 308, 857, 890, 5367, 5400
BalI TGGCCA 3 cut(s) 3430, 4942, 5540
BanI GGYRCC 4 cut(s) 32, 1918, 2013, 2209
BanII GRGCYC 2 cut(s) 4608, 6192
BarI GAAGNNNNNNTAC 6 cut(s) 371, 403, 2305, 2337, 3849, 3881
BbsI GAAGAC 3 cut(s) 1499, 3119, 3500
Bbv12I GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5857, 6192
BbvCI CCTCAGC 1 cut(s) 6588
BceAI ACGGC 4 cut(s) 28, 98, 1269, 1653
BcgI CGANNNNNNTGC 4 cut(s) 1427, 1461, 2449, 2483
BciVI GTATCC 1 cut(s) 5262
BclI TGATCA 4 cut(s) 2794, 3031, 4987, 5950
BcnI CCSGG 2 cut(s) 3346, 3347
BcoDI GTCTC 7 cut(s) 188, 237, 2667, 3260, 4535, 4844, 5723
BcuI ACTAGT 2 cut(s) 3892, 6206
BfmI CTRYAG 9 cut(s) 163, 1760, 2325, 2853, 3705, 4438, 5304, 5553, 5696
BfoI RGCGCY 2 cut(s) 163, 2017
BfuAI ACCTGC 2 cut(s) 355, 1078
BfuI GTATCC 1 cut(s) 5262
BglII AGATCT 4 cut(s) 1268, 1674, 4598, 6624
BlnI CCTAGG 1 cut(s) 998
BlpI GCTNAGC 2 cut(s) 69, 2385
BmcAI AGTACT 2 cut(s) 5009, 6941
BmeRI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
BmeT110I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
BmgBI CACGTC 1 cut(s) 1127
BmrI ACTGGG 3 cut(s) 408, 4713, 5481
BmuI ACTGGG 3 cut(s) 408, 4713, 5481
BoxI GACNNNNGTC 1 cut(s) 2823
BpiI GAAGAC 3 cut(s) 1499, 3119, 3500
BplI GAGNNNNNCTC 4 cut(s) 1183, 1215, 1221, 1253
BpmI CTGGAG 4 cut(s) 629, 1248, 2576, 5666
Bpu10I CCTNAGC 1 cut(s) 6588
Bpu1102I GCTNAGC 2 cut(s) 69, 2385
BpuEI CTTGAG 5 cut(s) 2789, 4203, 5705, 6882, 6995
BpuMI CCSGG 2 cut(s) 3346, 3347
Bsa29I ATCGAT 1 cut(s) 4565
BsaBI GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BsaHI GRCGYC 2 cut(s) 2014, 4234
BsaI GGTCTC 3 cut(s) 237, 3260, 4535
BsaWI WCCGGW 2 cut(s) 35, 217
BsaXI ACNNNNNCTCC 2 cut(s) 4330, 4360
Bse118I RCCGGY 4 cut(s) 35, 744, 4308, 6957
Bse21I CCTNAGG 3 cut(s) 2121, 6441, 6600
Bse3DI GCAATG 3 cut(s) 5223, 6462, 6650
Bse8I GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BseAI TCCGGA 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 4565
BseJI GATNNNNATC 5 cut(s) 585, 1062, 2787, 2799, 3468
BseMI GCAATG 3 cut(s) 5223, 6462, 6650
BseRI GAGGAG 5 cut(s) 2453, 2862, 6072, 6588, 7007
BseSI GKGCMC 4 cut(s) 1921, 3315, 4768, 5857
BseX3I CGGCCG 1 cut(s) 6959
BseYI CCCAGC 2 cut(s) 1106, 4176
BsgI GTGCAG 4 cut(s) 1308, 4709, 4877, 5553
Bsh1236I CGCG 2 cut(s) 3059, 3123
Bsh1285I CGRYCG 1 cut(s) 6962
BshNI GGYRCC 4 cut(s) 32, 1918, 2013, 2209
BshTI ACCGGT 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 4565
BsiEI CGRYCG 1 cut(s) 6962
BsiHKAI GWGCWC 6 cut(s) 120, 304, 3315, 4768, 5857, 6192
BsiHKCI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
BsiSI CCGG 8 cut(s) 36, 98, 218, 745, 1276, 3346, 4309, 6958
BslFI GGGAC 6 cut(s) 266, 1624, 2939, 3266, 6756, 6940
BsmAI GTCTC 7 cut(s) 188, 237, 2667, 3260, 4535, 4844, 5723
BsmBI CGTCTC 1 cut(s) 188
BsmFI GGGAC 6 cut(s) 266, 1624, 2939, 3266, 6756, 6940
BsmI GAATGC 7 cut(s) 525, 843, 971, 1073, 3644, 5637, 5825
Bso31I GGTCTC 3 cut(s) 237, 3260, 4535
BsoBI CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
Bsp1286I GDGCHC 8 cut(s) 120, 304, 1921, 3315, 4608, 4768, 5857, 6192
Bsp13I TCCGGA 1 cut(s) 217
Bsp1407I TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
Bsp1720I GCTNAGC 2 cut(s) 69, 2385
Bsp19I CCATGG 4 cut(s) 247, 1032, 4017, 6863
BspDI ATCGAT 1 cut(s) 4565
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 2 cut(s) 3059, 3123
BspHI TCATGA 5 cut(s) 277, 3334, 3453, 4105, 6946
BspMAI CTGCAG 5 cut(s) 1764, 2857, 3709, 5557, 5700
BspMI ACCTGC 2 cut(s) 355, 1078
BspQI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
BspT107I GGYRCC 4 cut(s) 32, 1918, 2013, 2209
BspTNI GGTCTC 3 cut(s) 237, 3260, 4535
BsrDI GCAATG 3 cut(s) 5223, 6462, 6650
BsrFI RCCGGY 4 cut(s) 35, 744, 4308, 6957
BsrGI TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
BssAI RCCGGY 4 cut(s) 35, 744, 4308, 6957
BssNI GRCGYC 2 cut(s) 2014, 4234
BstACI GRCGYC 2 cut(s) 2014, 4234
BstAPI GCANNNNNTGC 1 cut(s) 4196
BstAUI TGTACA 5 cut(s) 1041, 2730, 3148, 3865, 5020
BstDSI CCRYGG 4 cut(s) 247, 1032, 4017, 6863
BstEII GGTNACC 2 cut(s) 942, 6779
BstFNI CGCG 2 cut(s) 3059, 3123
BstH2I RGCGCY 2 cut(s) 163, 2017
BstHHI GCGC 4 cut(s) 162, 177, 2016, 5580
BstMAI GTCTC 7 cut(s) 188, 237, 2667, 3260, 4535, 4844, 5723
BstMCI CGRYCG 1 cut(s) 6962
BstNSI RCATGY 6 cut(s) 214, 227, 259, 1537, 3595, 5107
BstPAI GACNNNNGTC 1 cut(s) 2823
BstPI GGTNACC 2 cut(s) 942, 6779
BstSFI CTRYAG 9 cut(s) 163, 1760, 2325, 2853, 3705, 4438, 5304, 5553, 5696
BstSLI GKGCMC 4 cut(s) 1921, 3315, 4768, 5857
BstUI CGCG 2 cut(s) 3059, 3123
BstV2I GAAGAC 3 cut(s) 1499, 3119, 3500
BstXI CCANNNNNNTGG 5 cut(s) 357, 398, 5083, 5333, 6480
BstZI CGGCCG 1 cut(s) 6959
Bsu15I ATCGAT 1 cut(s) 4565
Bsu36I CCTNAGG 3 cut(s) 2121, 6441, 6600
BsuI GTATCC 1 cut(s) 5262
BsuTUI ATCGAT 1 cut(s) 4565
BtgI CCRYGG 4 cut(s) 247, 1032, 4017, 6863
BtgZI GCGATG 1 cut(s) 2129
BtrI CACGTC 1 cut(s) 1127
BtsI GCAGTG 2 cut(s) 2948, 6485
BveI ACCTGC 2 cut(s) 355, 1078
CaiI CAGNNNCTG 1 cut(s) 1226
CciI TCATGA 5 cut(s) 277, 3334, 3453, 4105, 6946
CfoI GCGC 4 cut(s) 162, 177, 2016, 5580
Cfr10I RCCGGY 4 cut(s) 35, 744, 4308, 6957
Cfr9I CCCGGG 1 cut(s) 3345
ClaI ATCGAT 1 cut(s) 4565
CseI GACGC 5 cut(s) 205, 1814, 2663, 4223, 5513
CsiI ACCWGGT 2 cut(s) 2212, 4365
CspAI ACCGGT 1 cut(s) 35
CspCI CAANNNNNGTGG 4 cut(s) 2010, 2045, 2786, 2821
DinI GGCGCC 1 cut(s) 2015
DraI TTTAAA 2 cut(s) 514, 4501
DrdI GACNNNNNNGTC 2 cut(s) 5523, 5906
DriI GACNNNNNGTC 3 cut(s) 808, 2340, 5104
DseDI GACNNNNNNGTC 2 cut(s) 5523, 5906
EaeI YGGCCR 6 cut(s) 13, 1273, 3428, 4940, 5538, 6959
EagI CGGCCG 1 cut(s) 6959
Eam1105I GACNNNNNGTC 3 cut(s) 808, 2340, 5104
EciI GGCGGA 2 cut(s) 42, 4982
Ecl136II GAGCTC 1 cut(s) 6190
EclXI CGGCCG 1 cut(s) 6959
Eco24I GRGCYC 2 cut(s) 4608, 6192
Eco31I GGTCTC 3 cut(s) 237, 3260, 4535
Eco32I GATATC 3 cut(s) 3365, 4743, 6058
Eco47III AGCGCT 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 6959
Eco53kI GAGCTC 1 cut(s) 6190
Eco57I CTGAAG 9 cut(s) 1074, 1518, 1619, 2399, 2942, 3113, 3571, 5192, 5507
Eco81I CCTNAGG 3 cut(s) 2121, 6441, 6600
Eco88I CYCGRG 5 cut(s) 338, 3195, 3345, 5070, 6521
Eco91I GGTNACC 2 cut(s) 942, 6779
EcoICRI GAGCTC 1 cut(s) 6190
EcoO109I RGGNCCY 1 cut(s) 1898
EcoO65I GGTNACC 2 cut(s) 942, 6779
EcoRI GAATTC 4 cut(s) 3940, 4163, 4275, 6307
EcoRV GATATC 3 cut(s) 3365, 4743, 6058
EcoT22I ATGCAT 2 cut(s) 2100, 4719
EcoT38I GRGCYC 2 cut(s) 4608, 6192
EgeI GGCGCC 1 cut(s) 2015
EheI GGCGCC 1 cut(s) 2015
Esp3I CGTCTC 1 cut(s) 188
FaqI GGGAC 6 cut(s) 266, 1624, 2939, 3266, 6756, 6940
FauI CCCGC 3 cut(s) 1495, 2655, 5253
FauNDI CATATG 4 cut(s) 2425, 2921, 4324, 6374
FbaI TGATCA 4 cut(s) 2794, 3031, 4987, 5950
FblI GTMKAC 2 cut(s) 505, 3788
FriOI GRGCYC 2 cut(s) 4608, 6192
GlaI GCGC 4 cut(s) 161, 176, 2015, 5579
GsaI CCCAGC 2 cut(s) 1110, 4180
GsuI CTGGAG 4 cut(s) 629, 1248, 2576, 5666
HaeII RGCGCY 2 cut(s) 163, 2017
HapII CCGG 8 cut(s) 36, 98, 218, 745, 1276, 3346, 4309, 6958
HgaI GACGC 5 cut(s) 205, 1814, 2663, 4223, 5513
HhaI GCGC 4 cut(s) 162, 177, 2016, 5580
Hin1I GRCGYC 2 cut(s) 2014, 4234
Hin6I GCGC 4 cut(s) 160, 175, 2014, 5578
HinP1I GCGC 4 cut(s) 160, 175, 2014, 5578
HincII GTYRAC 8 cut(s) 506, 1686, 1791, 2399, 3811, 6292, 6705, 6751
HindII GTYRAC 8 cut(s) 506, 1686, 1791, 2399, 3811, 6292, 6705, 6751
HindIII AAGCTT 3 cut(s) 332, 4418, 6920
HpaI GTTAAC 1 cut(s) 6705
HpaII CCGG 8 cut(s) 36, 98, 218, 745, 1276, 3346, 4309, 6958
Hpy99I CGWCG 3 cut(s) 49, 507, 1128
HpyCH4IV ACGT 9 cut(s) 286, 1126, 2253, 2305, 2395, 2824, 3019, 5062, 6018
HpySE526I ACGT 9 cut(s) 286, 1126, 2253, 2305, 2395, 2824, 3019, 5062, 6018
Hsp92I GRCGYC 2 cut(s) 2014, 4234
HspAI GCGC 4 cut(s) 160, 175, 2014, 5578
KasI GGCGCC 1 cut(s) 2013
Kpn2I TCCGGA 1 cut(s) 217
KpnI GGTACC 1 cut(s) 2213
Ksp22I TGATCA 4 cut(s) 2794, 3031, 4987, 5950
KspAI GTTAAC 1 cut(s) 6705
LguI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
LmnI GCTCC 8 cut(s) 220, 299, 361, 1120, 2077, 2863, 4969, 6068
MabI ACCWGGT 2 cut(s) 2212, 4365
MaeII ACGT 9 cut(s) 286, 1126, 2253, 2305, 2395, 2824, 3019, 5062, 6018
MhlI GDGCHC 8 cut(s) 120, 304, 1921, 3315, 4608, 4768, 5857, 6192
MlsI TGGCCA 3 cut(s) 3430, 4942, 5540
MluNI TGGCCA 3 cut(s) 3430, 4942, 5540
Mly113I GGCGCC 1 cut(s) 2014
MlyI GAGTC 9 cut(s) 818, 1273, 2489, 3103, 3187, 3675, 4111, 4390, 5614
MmeI TCCRAC 5 cut(s) 594, 2457, 4674, 5640, 6533
Mox20I TGGCCA 3 cut(s) 3430, 4942, 5540
Mph1103I ATGCAT 2 cut(s) 2100, 4719
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 4 cut(s) 269, 3236, 4869, 6368
MscI TGGCCA 3 cut(s) 3430, 4942, 5540
Msp20I TGGCCA 3 cut(s) 3430, 4942, 5540
MspA1I CMGCKG 6 cut(s) 760, 871, 1226, 4120, 5479, 6145
MspI CCGG 8 cut(s) 36, 98, 218, 745, 1276, 3346, 4309, 6958
Mva1269I GAATGC 7 cut(s) 525, 843, 971, 1073, 3644, 5637, 5825
MvnI CGCG 2 cut(s) 3059, 3123
NarI GGCGCC 1 cut(s) 2014
NciI CCSGG 2 cut(s) 3346, 3347
NcoI CCATGG 4 cut(s) 247, 1032, 4017, 6863
NdeI CATATG 4 cut(s) 2425, 2921, 4324, 6374
NmeAIII GCCGAG 3 cut(s) 133, 1978, 5812
NsiI ATGCAT 2 cut(s) 2100, 4719
NspI RCATGY 6 cut(s) 214, 227, 259, 1537, 3595, 5107
OliI CACNNNNGTG 9 cut(s) 1924, 3260, 4639, 4771, 5129, 5502, 5504, 6478, 7007
PaeR7I CTCGAG 2 cut(s) 338, 3195
PagI TCATGA 5 cut(s) 277, 3334, 3453, 4105, 6946
PasI CCCWGGG 1 cut(s) 357
PciI ACATGT 2 cut(s) 255, 5103
PciSI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
PcsI WCGNNNNNNNCGW 2 cut(s) 2671, 5068
PctI GAATGC 7 cut(s) 525, 843, 971, 1073, 3644, 5637, 5825
PdmI GAANNNNTTC 4 cut(s) 269, 3236, 4869, 6368
PflFI GACNNNGTC 1 cut(s) 6040
PflMI CCANNNNNTGG 3 cut(s) 5471, 5659, 6645
PinAI ACCGGT 1 cut(s) 35
PleI GAGTC 9 cut(s) 817, 1273, 2488, 3102, 3187, 3674, 4110, 4390, 5614
PluTI GGCGCC 1 cut(s) 2017
PpsI GAGTC 9 cut(s) 817, 1273, 2488, 3102, 3187, 3674, 4110, 4390, 5614
PpuMI RGGWCCY 1 cut(s) 1898
PscI ACATGT 2 cut(s) 255, 5103
PshAI GACNNNNGTC 1 cut(s) 2823
PshBI ATTAAT 2 cut(s) 687, 4656
PsiI TTATAA 2 cut(s) 1530, 2886
Psp124BI GAGCTC 1 cut(s) 6192
Psp1406I AACGTT 1 cut(s) 5062
Psp5II RGGWCCY 1 cut(s) 1898
PspEI GGTNACC 2 cut(s) 942, 6779
PspFI CCCAGC 2 cut(s) 1106, 4176
PspPPI RGGWCCY 1 cut(s) 1898
PspXI VCTCGAGB 1 cut(s) 3195
PstI CTGCAG 5 cut(s) 1764, 2857, 3709, 5557, 5700
PstNI CAGNNNCTG 1 cut(s) 1226
PsyI GACNNNGTC 1 cut(s) 6040
PvuII CAGCTG 5 cut(s) 760, 1226, 4120, 5479, 6145
SacI GAGCTC 1 cut(s) 6192
SalI GTCGAC 1 cut(s) 504
SapI GCTCTTC 4 cut(s) 2607, 3590, 5589, 6522
ScaI AGTACT 2 cut(s) 5009, 6941
SchI GAGTC 9 cut(s) 818, 1273, 2489, 3103, 3187, 3675, 4111, 4390, 5614
SduI GDGCHC 8 cut(s) 120, 304, 1921, 3315, 4608, 4768, 5857, 6192
SexAI ACCWGGT 2 cut(s) 2212, 4365
SfcI CTRYAG 9 cut(s) 163, 1760, 2325, 2853, 3705, 4438, 5304, 5553, 5696
SfoI GGCGCC 1 cut(s) 2015
Sfr274I CTCGAG 2 cut(s) 338, 3195
SgrAI CRCCGGYG 1 cut(s) 35
SlaI CTCGAG 2 cut(s) 338, 3195
SmaI CCCGGG 1 cut(s) 3347
SmlI CTYRAG 7 cut(s) 338, 2768, 3195, 4218, 5720, 6897, 6974
SmoI CTYRAG 7 cut(s) 338, 2768, 3195, 4218, 5720, 6897, 6974
SpeI ACTAGT 2 cut(s) 3892, 6206
SspDI GGCGCC 1 cut(s) 2013
SspI AATATT 3 cut(s) 2197, 3155, 4758
SstI GAGCTC 1 cut(s) 6192
TaiI ACGT 9 cut(s) 289, 1129, 2256, 2308, 2398, 2827, 3022, 5065, 6021
TaqII GACCGA 1 cut(s) 930
TauI GCSGC 4 cut(s) 28, 94, 2869, 4931
TspGWI ACGGA 6 cut(s) 263, 359, 1709, 3842, 4751, 5287
TspMI CCCGGG 1 cut(s) 3345
Tth111I GACNNNGTC 1 cut(s) 6040
Van91I CCANNNNNTGG 3 cut(s) 5471, 5659, 6645
VneI GTGCAC 3 cut(s) 3311, 4764, 5853
VspI ATTAAT 2 cut(s) 687, 4656
XbaI TCTAGA 1 cut(s) 592
XceI RCATGY 6 cut(s) 214, 227, 259, 1537, 3595, 5107
XcmI CCANNNNNNNNNTGG 1 cut(s) 5928
XhoI CTCGAG 2 cut(s) 338, 3195
XmaI CCCGGG 1 cut(s) 3345
XmaJI CCTAGG 1 cut(s) 998
XmiI GTMKAC 2 cut(s) 505, 3788
XmnI GAANNNNTTC 4 cut(s) 269, 3236, 4869, 6368
ZrmI AGTACT 2 cut(s) 5009, 6941
Zsp2I ATGCAT 2 cut(s) 2100, 4719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.