Rmu_sc0000674.1_g000029

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000674.1
OG0000798
Physical Location & Seq
Forward (+)
148261 .. 155271
7011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000674.1_g000029.1.cds

Sequence Viewer

Length: 7011 bp
atgtggtacaacgccgaaatttcaccgccggggacgggaaatacctcgattccatcggcggtggctcaaccctgtcttccgtcaccggccgatcttgaagagaaagcccgcagatggcagcagctttcctccctcaagtggtccaatcgccacggcgaacggcgcgccgtcgattcgcaaaaacaggacatgcctccggagcatgtgaggaagatcgtgaaagagcacggcgacatgtcgtcgaagaagtaccggcacgacaagcgggtctacctgggagctctgaagttcgttcctcacgccgtgtacaagcttctcgagaatatgccgatgccgtgggagcaggtccgtaatgtgaaggtgctgtaccatattaccggcgcagttaccttcgtcaacgagactccctgggttgtggagccgatctacatggctcaatgggccacaatgtggatcatgatgaggagggagaagcgggatcgccggcatttcaagaggatgaggtttccgccgttcgatgatgaggagcctgtgttggattatgctgataacttgttggatgttgagcctcttgagccgattcagctcgagcttgatgccgaagaggattcggctgtgtatgactggttttatgaccataaacctcttgtgaagactaagctgatcaacggcccgacctaccggaagtggcagctctccctgccgattatggaagccctttaccggctttcaggacaggtaatttccgatttgactgatcggaactatttctatttgtttgataaggagtcgtttctgacagccaaggcattgaatatgtgtataccaggtgggccgaagttcgagccattgtttagggatatggagcaaggggaggaggattggaatgagtttaatgacataaacaagctcattattcggtctccgctgagatcagagtacaaaattgcattccctaatctttacaacaacaggccaaggaaggtaaagctctctgtttatcacacaccaatggttatgcatattaaaactgaagatcctgacctgcctgcattttattatgatccattgctaaatccaataccatgcactaacaggagtagttatgataatagtgaggaagatggtgattttgttttgcctgaaggggtggagcctcttttgagtcatactcagctttatactgacaccactgctgctgggatttccctgtattttgccccacgcccttttaacttgaggtctggtcggactcgtcgtgcagaggatatacctcttgtgtcagagtggtttaaggagcattgtcctcggtcatatcctgttaaggttcgagtcagctatcagaagttgttgaaatcgtttgtgttgaatgagctgcatcgtaggcctcccaaggcacaaaagaagaagagcttgtttcggtctcttcaggctaccaagtttttccaaactactgagcttgattgggttgaagcgggtcttcaactctgtaggcagggtcataacatgctaaatctgctgatacataggaaaggtctgaactatcttcaccttgattataattttaatctcaagcctgtgaaaaccctgacaacaaaagagcgtaagaaatctcggtttggtaatccttttcatctgttgcgtgaaatcttgcggttaacaaagcttgtagttgatgcccaggtccagttccgtttgggtaatattgatgcctttcaattggctgatggtctgcagtacatattttctcatgttggtcagttaactggaatgtaccgttacaagtataggttgatgcggcagattagaatgagcaaagatttgaagcacttgatttactaccgtttcaatactgggccggtcgggaaagggccaggatgtggattctgggcaccaatgtggagggtgtggttgtccttccttcgtggcatggtccctcttctggaacagtggttgggaaatttacttgctagacaatttgaaggacgccattctgtggcaaagacagtaactaagcagcgtgttgaaagcaatttcgacttggagtttcgagcagatgtcttgaaagatattctggatgccatgccagtgggcatgaagcaaaataaggcgaaaacaattatgcagcatctcagtgaggcatggcgttgttggaaagcaaatattccttggaaggttcctggtatgcctgctccggttgagaatataattcttcgttatgtcaagtcaaaggcaaattggtggacacatggtgctcattataaccgtgaacgaattagaagaggtgctacagtggataagacagtttgtcgaaagaatcttggaagattaactcgcctttggctaaagggagagcaggagcgacagcacaattatctcaaagatggtccatatatttcaccagaagaagtagtagcaatttactcaacaacagtgcattggttggagtcaagaaatttccaacatattccatttccccctttgttgtataagcatgataccaagctacttatccttgctctggagaggttgaaggaatcttatactgcggcagtaaaattgaaccagcatcaaagagaggagttgggtctcattgaacaagcctatgataacccccatgaggcattgatacggataaaacgtcacttgatctcacagcgtgccttcaaagacgtgagcatagagttcatggatctgtacagccatcatattccagtttttgagattgaccctcttgagaagattacagatgcatatcttgatcaatatctatggtatgaggctgacaaacgtcagctcttcccgaactggatcaagcctgcagactcggagccacctccacttttggtctataaatggtgtcaggggataaacaatttagagagtatatgggacacaagtgagggggagtgtgttgtgatgcttcagacaaaacttgagaggttatctgagaagattgacttgacattgctaaataggcttctacgtcttgttcttgaccataacattgctgattatatcactgcaaaaaacaatgtggtattgtcatacaaagatatgagtcatacaaattcatatggtcttatacggggtctccagcctgcatcatttgttgtacagtattatgggcttgttttggatctaatgcttcttggattgactcgggccaatgaaattgcaggtccacagcagaagccaaatgagttcatgacttatggggccacaaaggttgagacttgccatcctatcaggctgtacgctcgatacatagacagggtgcatatcttgttccgcttcactcaggaggaggctcgggacctcatacagcgatatcttaccgagcatccagatcccaataatgaaagtatggttggatataataataagaagtgctgggcaagagatgcaaggatgaggctcctgaaacatgatgtcaatcttggaaggagtgtcttttggaatgtcaagaaccatctccctcgaagcatcacaactttggaatgggaggacagttttgtttctgtttacagcaaggataatccaaatttgcttttcagcatgtgtggatttgaagtaagaatacttcccaagataagaatgagtacgaaggaacaccaaagagatggagtttggaacttgcaaaatgaacagacaaaggaaaggactgctgttgcttttttacgtgttgaggatgaatacatgaaggcgtttgagaatcgtgtaaggcagatccttatgtcctcagggtctacaacattcacaaaagttatcaacaaatggaatactgcccttattggtcttatgacttacttccgtgaagcagttgtacatacagcggagctgttagatttactggtcaaatgtgaaaataaaattcagacccgcattaagattggattgaattcaaagatgccaagcaggttcccacctgtcattttttacacgccaaaagaaattggaggacttggcatgttatctatgggtcacatattgattccacagagtgacctcagatactctcggcagacagaaatcggtgtgacacactttaggagtggaatgagtcatgaagaagagcagctgattccaaatctttaccgttacatacaaccatgggagagtgagtttatagattcacagcgtgtttgggcagaatatgcactaaaaagacaggaagctatggaacagaatcggcgtcttacccttgaagatttggaagattcatgggataggggaataccacgcataaatacattgttccagaaggatcgacacactttagcatatgacaaagggtggagagttcgcacagattttaagcagtaccagatcttgaagcaaaatcctttctggtggacacaccaaaggcatgatggaaaattgtggaatttgaataattatagaactgatgtgatccaagctcttggaggagttgatggaatacttgagcacaccttattcaaaggaacatacttccctacttgggagggtctcttttgggagaaggcatctggatttgaggaatcgatgaagtataaaaagttgactaatgcccaaagatctggcctcaaccaaatccccaatcgtaggtttacactttggtggtcgcctaccataaatcgagccaatgtatatgtcggttttcaagtacagctggatctgacaggaatctttatgcatgggaagatcccaaccttgaagatatcgttgattcagatattccgtgcacacttgtggcagaagattcatgaaagtgttgtgatggacctttgtcaggttttggatcaggagttggatgctttggaaattgagactgtgcagaaggaaaccatacatcccaggaagagttacaaaatgaacagctcttgtgcagacattctcctcttttctgctcataaatggcaaatgtcgaaacctagtcttgtggctgagccgaaggatgagtatgataaaaaaacaaacaacaagtactggatagacgtgcagctccgttggggggattatgattctcatgatattgaacgttacacccgggcaaagtttatggactatacaactgataacatgtctatctatccttctccgatgggagtgatgattggtattgatttagcctataatgtgcattctgcatttggtaactggtttcccggatcaaaaccactgattcaacaggcaatgaacaagatcatgaagtcaaatccggctttatatgtgttgagggagcgcataaggaaaggattgcagttgtattcctcggagcctacagagccatatttgtcttcccaaaactatggggagctcttcagcaatcaaattaaatggtttgttgacgatacaaatgtgtatcgtgttacaatgcacaagacttttgacggaaatcttgttacaaaacccatcaatggtgctatcttcatgttcaatccagggactgggcagctgttcttgaaggttatccacacaagtgtgtgggcaggacagaagcgacttgggcagttggctaaatggaaaactgcagaagaagttgctgctcttgtgcgttctctacctgttgaagaacagccaaagaaaattattgtgactcgtaagggaatgctcgacccattggaggttcatttactggattttcccaacatagtcataaaaggaagtgagctacagcttccttttcaggtttgcttgaaaattgagaagttgaatgatctggtactaaaggctactgagccacagatggttctttttaatgtttacgatgattggttgaagagtgtttcatcatacactgcattctctcggctcattctgattcttcgggcactgcatgtgaacagagacaaggcaagtatgttgttgaagcctgaccgaacagtcattagtgagccacaccacatttggccttctctttctgatgagcaatggatgaaggttgagcgtgctctaacagatctcatactgtcagactatgcgaaaaagaataatgtgaatacttcagccctcacacagtctgaaattcgtgatataatacttggagcagagattactccaccttctcaacagaggcaagagatagcagagattgagaaacaggctaaaggagttagtcagctgacagcagtcaccatgaggaccacaaacgtgcatggagatgagcttatttccaccactacgagtcaatatgagcaagctgcattcaggtccaaaactgattggcgtgtgagagcaatatcagctacaaatctacatcttcgtgtcaaccatatttatgtgagttcagaagatacaaaggaaacaggctacacttacatcatgccaatgaacattttgaaaaaatttatctgtgttgctgatctgaggacacaagttgctggttacttgtatggtataagcccacctgacaaccctcagattaaggaaatttgctgcattgtgatgccaccacagcggggcacccaccagcaagttcatcttccatcagctcttcctgagcatgatttcctcaattatttggaacctctgggatggatgcacacccagccaaatgagctacctcagctttctcctcaggatctcaccactcatgctaacatgttggagaatggcaagcaatgggatagggagaaatgcattattatgacctgcgcttttactccaggttcctgctcgttaactgcgtataaacttactccttctggttatgagtggggatgttgttctaacaagaacagcagaagaaacaataatccactagatggataccttccaactcattatgagaaggtccagatgcttctcagtgatcgcttccttggtgtctacatggttcctgataattgtccatggaacttcaatttcatgggagtgaggcatgcatgtgacatgaagtatggcatcaagcttggaccacccaaggagtactatcacgaggaacaccgaccaacccatttcatggagttcagcgaactagataaggatgacccgatggagttagatcgagattatgcctacgcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2336

Amino Acids

273.0

Weight (kDa)

8.9

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1560, 2262
AasI GACNNNNNNGTC 2 cut(s) 266, 5867
Acc36I ACCTGC 5 cut(s) 334, 1053, 3170, 3909, 6644
AccB1I GGYRCC 2 cut(s) 1891, 6446
AccB7I CCANNNNNTGG 6 cut(s) 450, 1880, 1996, 5432, 6749, 6946
AccI GTMKAC 4 cut(s) 270, 823, 3749, 6813
AccII CGCG 1 cut(s) 165
AccIII TCCGGA 1 cut(s) 196
AclI AACGTT 1 cut(s) 5023
AcoI YGGCCR 1 cut(s) 87
AcuI CTGAAG 7 cut(s) 305, 1053, 1164, 1412, 2909, 5290, 5973
AcyI GRCGYC 2 cut(s) 1987, 4195
AdeI CACNNNGTG 6 cut(s) 304, 450, 2252, 2660, 4004, 4142
AflIII ACRYGT 4 cut(s) 234, 3682, 5064, 6585
AhdI GACNNNNNGTC 2 cut(s) 238, 2307
AjiI CACGTC 2 cut(s) 2674, 4981
AjnI CCWGG 9 cut(s) 273, 407, 826, 1680, 1873, 2179, 4838, 5425, 6649
AjuI GAANNNNNNNTTGG 2 cut(s) 3354, 3386
AleI CACNNNNGTG 6 cut(s) 1897, 4600, 4732, 5463, 5465, 6134
Alw21I GWGCWC 7 cut(s) 228, 283, 2257, 4452, 4729, 5304, 5938
Alw26I GTCTC 9 cut(s) 395, 927, 1428, 2594, 3098, 3227, 4496, 4805, 5826
Alw44I GTGCAC 1 cut(s) 4725
AlwNI CAGNNNCTG 1 cut(s) 5432
Ama87I CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
Aor13HI TCCGGA 1 cut(s) 196
ApaLI GTGCAC 1 cut(s) 4725
AscI GGCGCGCC 1 cut(s) 163
Asp700I GAANNNNTTC 1 cut(s) 767
AspLEI GCGC 5 cut(s) 165, 167, 383, 5229, 6641
AsuC2I CCSGG 4 cut(s) 30, 5032, 5033, 5151
AsuHPI GGTGA 7 cut(s) 15, 75, 1139, 1541, 2391, 6109, 6562
AvaI CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
AxyI CCTNAGG 2 cut(s) 3742, 6561
BaeGI GKGCMC 4 cut(s) 1894, 4729, 5818, 6449
BaeI ACNNNNGTAYC 2 cut(s) 5328, 5361
BanI GGYRCC 2 cut(s) 1891, 6446
BanII GRGCYC 2 cut(s) 283, 5304
BauI CACGAG 1 cut(s) 6920
BbsI GAAGAC 4 cut(s) 68, 659, 1472, 5274
Bbv12I GWGCWC 7 cut(s) 228, 283, 2257, 4452, 4729, 5304, 5938
BbvCI CCTCAGC 1 cut(s) 6549
BceAI ACGGC 8 cut(s) 152, 169, 176, 244, 287, 319, 496, 685
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 1289, 1323
BciT130I CCWGG 9 cut(s) 275, 409, 828, 1682, 1875, 2181, 4840, 5427, 6651
BciVI GTATCC 1 cut(s) 6746
BclI TGATCA 2 cut(s) 663, 2761
BcnI CCSGG 4 cut(s) 30, 5032, 5033, 5151
BcoDI GTCTC 9 cut(s) 395, 927, 1428, 2594, 3098, 3227, 4496, 4805, 5826
BfaI CTAG 4 cut(s) 1971, 4917, 6746, 6962
BfmI CTRYAG 7 cut(s) 1489, 1733, 2289, 2820, 5265, 5514, 5657
BfuAI ACCTGC 5 cut(s) 334, 1053, 3170, 3909, 6644
BfuI GTATCC 1 cut(s) 6746
BglII AGATCT 3 cut(s) 4329, 4559, 5944
BlpI GCTNAGC 1 cut(s) 4929
BmcAI AGTACT 2 cut(s) 4970, 6914
BmeRI GACNNNNNGTC 2 cut(s) 238, 2307
BmeT110I CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
BmgBI CACGTC 2 cut(s) 2674, 4981
BmrI ACTGGG 2 cut(s) 1863, 5442
BmuI ACTGGG 2 cut(s) 1863, 5442
BoxI GACNNNNGTC 3 cut(s) 2790, 4770, 6113
BpiI GAAGAC 4 cut(s) 68, 659, 1472, 5274
BplI GAGNNNNNCTC 2 cut(s) 1156, 1188
BpmI CTGGAG 3 cut(s) 2543, 3080, 6633
Bpu10I CCTNAGC 2 cut(s) 6483, 6549
Bpu1102I GCTNAGC 1 cut(s) 4929
BpuEI CTTGAG 7 cut(s) 119, 593, 1258, 1556, 2756, 2957, 4466
BpuMI CCSGG 4 cut(s) 30, 5032, 5033, 5151
Bsa29I ATCGAT 1 cut(s) 4526
BsaAI YACGTR 1 cut(s) 3683
BsaBI GATNNNNATC 3 cut(s) 2754, 2766, 3435
BsaHI GRCGYC 2 cut(s) 1987, 4195
BsaI GGTCTC 5 cut(s) 927, 1428, 2594, 3098, 4496
BsaWI WCCGGW 3 cut(s) 196, 681, 2194
BsaXI ACNNNNNCTCC 6 cut(s) 518, 548, 3078, 3108, 4109, 4139
Bse118I RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
Bse21I CCTNAGG 2 cut(s) 3742, 6561
Bse3DI GCAATG 6 cut(s) 1067, 2966, 3006, 5184, 5921, 6611
Bse8I GATNNNNATC 3 cut(s) 2754, 2766, 3435
BseAI TCCGGA 1 cut(s) 196
BseBI CCWGG 9 cut(s) 275, 409, 828, 1682, 1875, 2181, 4840, 5427, 6651
BseCI ATCGAT 1 cut(s) 4526
BseJI GATNNNNATC 3 cut(s) 2754, 2766, 3435
BseMI GCAATG 6 cut(s) 1067, 2966, 3006, 5184, 5921, 6611
BsePI GCGCGC 1 cut(s) 163
BseRI GAGGAG 8 cut(s) 478, 539, 890, 2594, 3320, 4443, 4871, 6549
BseSI GKGCMC 4 cut(s) 1894, 4729, 5818, 6449
BseX3I CGGCCG 1 cut(s) 87
BseYI CCCAGC 3 cut(s) 1199, 3393, 6531
BsgI GTGCAG 4 cut(s) 1281, 4838, 4890, 5003
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 2 cut(s) 90, 1863
BshNI GGYRCC 2 cut(s) 1891, 6446
BshVI ATCGAT 1 cut(s) 4526
BsiEI CGRYCG 2 cut(s) 90, 1863
BsiHKAI GWGCWC 7 cut(s) 228, 283, 2257, 4452, 4729, 5304, 5938
BsiHKCI CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
BslFI GGGAC 5 cut(s) 46, 1919, 2906, 3329, 5443
BsmAI GTCTC 9 cut(s) 395, 927, 1428, 2594, 3098, 3227, 4496, 4805, 5826
BsmFI GGGAC 5 cut(s) 46, 1919, 2906, 3329, 5443
BsmI GAATGC 5 cut(s) 950, 5125, 5598, 5786, 6188
Bso31I GGTCTC 5 cut(s) 927, 1428, 2594, 3098, 4496
BsoBI CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
Bsp13I TCCGGA 1 cut(s) 196
Bsp1407I TGTACA 4 cut(s) 306, 2697, 3115, 3826
Bsp1720I GCTNAGC 1 cut(s) 4929
Bsp19I CCATGG 2 cut(s) 4112, 6836
BspDI ATCGAT 1 cut(s) 4526
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 1 cut(s) 165
BspHI TCATGA 6 cut(s) 456, 3207, 4066, 4747, 5011, 5190
BspMAI CTGCAG 3 cut(s) 1737, 2824, 5518
BspMI ACCTGC 5 cut(s) 334, 1053, 3170, 3909, 6644
BspQI GCTCTTC 5 cut(s) 1403, 2804, 4068, 5309, 6483
BspT107I GGYRCC 2 cut(s) 1891, 6446
BspTNI GGTCTC 5 cut(s) 927, 1428, 2594, 3098, 4496
BsrDI GCAATG 6 cut(s) 1067, 2966, 3006, 5184, 5921, 6611
BsrFI RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
BsrGI TGTACA 4 cut(s) 306, 2697, 3115, 3826
BssAI RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
BssHII GCGCGC 1 cut(s) 163
BssNAI GTATAC 1 cut(s) 824
BssNI GRCGYC 2 cut(s) 1987, 4195
BssSI CACGAG 1 cut(s) 6920
BssT1I CCWWGG 8 cut(s) 804, 977, 1392, 2168, 4112, 6805, 6836, 6906
Bst1107I GTATAC 1 cut(s) 824
Bst2BI CACGAG 1 cut(s) 6920
Bst2UI CCWGG 9 cut(s) 275, 409, 828, 1682, 1875, 2181, 4840, 5427, 6651
BstACI GRCGYC 2 cut(s) 1987, 4195
BstAPI GCANNNNNTGC 2 cut(s) 3404, 4157
BstAUI TGTACA 4 cut(s) 306, 2697, 3115, 3826
BstBAI YACGTR 1 cut(s) 3683
BstDSI CCRYGG 4 cut(s) 151, 335, 4112, 6836
BstENI CCTNNNNNAGG 1 cut(s) 4772
BstFNI CGCG 1 cut(s) 165
BstHHI GCGC 5 cut(s) 165, 167, 383, 5229, 6641
BstMAI GTCTC 9 cut(s) 395, 927, 1428, 2594, 3098, 3227, 4496, 4805, 5826
BstMCI CGRYCG 2 cut(s) 90, 1863
BstNI CCWGG 9 cut(s) 275, 409, 828, 1682, 1875, 2181, 4840, 5427, 6651
BstPAI GACNNNNGTC 3 cut(s) 2790, 4770, 6113
BstSFI CTRYAG 7 cut(s) 1489, 1733, 2289, 2820, 5265, 5514, 5657
BstSLI GKGCMC 4 cut(s) 1894, 4729, 5818, 6449
BstUI CGCG 1 cut(s) 165
BstV2I GAAGAC 4 cut(s) 68, 659, 1472, 5274
BstXI CCANNNNNNTGG 5 cut(s) 2089, 3623, 4424, 4562, 5294
BstZ17I GTATAC 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 4526
Bsu36I CCTNAGG 2 cut(s) 3742, 6561
BsuI GTATCC 1 cut(s) 6746
BsuTUI ATCGAT 1 cut(s) 4526
BtgI CCRYGG 4 cut(s) 151, 335, 4112, 6836
BtrI CACGTC 2 cut(s) 2674, 4981
BtsI GCAGTG 4 cut(s) 1191, 3021, 5781, 5816
BveI ACCTGC 5 cut(s) 334, 1053, 3170, 3909, 6644
CaiI CAGNNNCTG 1 cut(s) 5432
CciI TCATGA 6 cut(s) 456, 3207, 4066, 4747, 5011, 5190
CfoI GCGC 5 cut(s) 165, 167, 383, 5229, 6641
Cfr10I RCCGGY 6 cut(s) 85, 252, 377, 483, 723, 1858
Cfr9I CCCGGG 1 cut(s) 5031
ClaI ATCGAT 1 cut(s) 4526
CseI GACGC 2 cut(s) 1995, 4184
CsiI ACCWGGT 1 cut(s) 826
CspCI CAANNNNNGTGG 2 cut(s) 3211, 3246
DraIII CACNNNGTG 6 cut(s) 304, 450, 2252, 2660, 4004, 4142
DrdI GACNNNNNNGTC 2 cut(s) 266, 5867
DriI GACNNNNNGTC 2 cut(s) 238, 2307
DseDI GACNNNNNNGTC 2 cut(s) 266, 5867
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
Eam1105I GACNNNNNGTC 2 cut(s) 238, 2307
EciI GGCGGA 1 cut(s) 498
Ecl136II GAGCTC 2 cut(s) 281, 5302
EclXI CGGCCG 1 cut(s) 87
Eco130I CCWWGG 8 cut(s) 804, 977, 1392, 2168, 4112, 6805, 6836, 6906
Eco147I AGGCCT 1 cut(s) 1387
Eco24I GRGCYC 2 cut(s) 283, 5304
Eco31I GGTCTC 5 cut(s) 927, 1428, 2594, 3098, 4496
Eco32I GATATC 2 cut(s) 3332, 4704
Eco52I CGGCCG 1 cut(s) 87
Eco53kI GAGCTC 2 cut(s) 281, 5302
Eco57I CTGAAG 7 cut(s) 305, 1053, 1164, 1412, 2909, 5290, 5973
Eco81I CCTNAGG 2 cut(s) 3742, 6561
Eco88I CYCGRG 5 cut(s) 317, 587, 3162, 3312, 5031
EcoICRI GAGCTC 2 cut(s) 281, 5302
EcoNI CCTNNNNNAGG 1 cut(s) 4772
EcoO109I RGGNCCY 1 cut(s) 3316
EcoRI GAATTC 1 cut(s) 3901
EcoRII CCWGG 9 cut(s) 273, 407, 826, 1680, 1873, 2179, 4838, 5425, 6649
EcoRV GATATC 2 cut(s) 3332, 4704
EcoT14I CCWWGG 8 cut(s) 804, 977, 1392, 2168, 4112, 6805, 6836, 6906
EcoT22I ATGCAT 5 cut(s) 1023, 2755, 4680, 6626, 6871
EcoT38I GRGCYC 2 cut(s) 283, 5304
ErhI CCWWGG 8 cut(s) 804, 977, 1392, 2168, 4112, 6805, 6836, 6906
FalI AAGNNNNNCTT 8 cut(s) 1397, 1429, 1464, 1496, 2945, 2977, 6450, 6482
FaqI GGGAC 5 cut(s) 46, 1919, 2906, 3329, 5443
FauI CCCGC 6 cut(s) 116, 258, 468, 1468, 3890, 6435
FauNDI CATATG 2 cut(s) 3076, 4285
FbaI TGATCA 2 cut(s) 663, 2761
FblI GTMKAC 4 cut(s) 270, 823, 3749, 6813
FriOI GRGCYC 2 cut(s) 283, 5304
FspBI CTAG 4 cut(s) 1971, 4917, 6746, 6962
GlaI GCGC 5 cut(s) 164, 166, 382, 5228, 6640
GsaI CCCAGC 3 cut(s) 1203, 3397, 6535
GsuI CTGGAG 3 cut(s) 2543, 3080, 6633
HgaI GACGC 2 cut(s) 1995, 4184
HhaI GCGC 5 cut(s) 165, 167, 383, 5229, 6641
Hin1I GRCGYC 2 cut(s) 1987, 4195
Hin6I GCGC 5 cut(s) 163, 165, 381, 5227, 6639
HinP1I GCGC 5 cut(s) 163, 165, 381, 5227, 6639
HincII GTYRAC 7 cut(s) 397, 1659, 1764, 4545, 5332, 6253, 6666
HindII GTYRAC 7 cut(s) 397, 1659, 1764, 4545, 5332, 6253, 6666
HindIII AAGCTT 3 cut(s) 311, 1664, 6893
HpaI GTTAAC 3 cut(s) 1659, 1764, 6666
HphI GGTGA 7 cut(s) 15, 75, 1139, 1541, 2391, 6109, 6562
Hpy99I CGWCG 3 cut(s) 173, 244, 1260
HpyCH4IV ACGT 8 cut(s) 2641, 2673, 2791, 2986, 3682, 4980, 5023, 6135
HpySE526I ACGT 8 cut(s) 2641, 2673, 2791, 2986, 3682, 4980, 5023, 6135
Hsp92I GRCGYC 2 cut(s) 1987, 4195
HspAI GCGC 5 cut(s) 163, 165, 381, 5227, 6639
Kpn2I TCCGGA 1 cut(s) 196
KroI GCCGGC 1 cut(s) 483
KroNI GCCGGC 1 cut(s) 485
Ksp22I TGATCA 2 cut(s) 663, 2761
KspAI GTTAAC 3 cut(s) 1659, 1764, 6666
LguI GCTCTTC 5 cut(s) 1403, 2804, 4068, 5309, 6483
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 4 cut(s) 1971, 4917, 6746, 6962
MaeII ACGT 8 cut(s) 2641, 2673, 2791, 2986, 3682, 4980, 5023, 6135
MfeI CAATTG 1 cut(s) 1718
Mph1103I ATGCAT 5 cut(s) 1023, 2755, 4680, 6626, 6871
MroI TCCGGA 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 767
MspA1I CMGCKG 7 cut(s) 928, 3836, 4081, 4654, 5440, 6106, 6442
MunI CAATTG 1 cut(s) 1718
Mva1269I GAATGC 5 cut(s) 950, 5125, 5598, 5786, 6188
MvaI CCWGG 9 cut(s) 275, 409, 828, 1682, 1875, 2181, 4840, 5427, 6651
MvnI CGCG 1 cut(s) 165
NaeI GCCGGC 1 cut(s) 485
NciI CCSGG 4 cut(s) 30, 5032, 5033, 5151
NcoI CCATGG 2 cut(s) 4112, 6836
NdeI CATATG 2 cut(s) 3076, 4285
NgoMIV GCCGGC 1 cut(s) 483
NmeAIII GCCGAG 2 cut(s) 4000, 5773
NmuCI GTSAC 8 cut(s) 81, 2642, 3983, 4004, 4039, 5578, 6115, 6872
NsiI ATGCAT 5 cut(s) 1023, 2755, 4680, 6626, 6871
OliI CACNNNNGTG 6 cut(s) 1897, 4600, 4732, 5463, 5465, 6134
PaeI GCATGC 1 cut(s) 6869
PaeR7I CTCGAG 2 cut(s) 317, 587
PagI TCATGA 6 cut(s) 456, 3207, 4066, 4747, 5011, 5190
PalAI GGCGCGCC 1 cut(s) 163
PasI CCCWGGG 1 cut(s) 408
PauI GCGCGC 1 cut(s) 163
PceI AGGCCT 1 cut(s) 1387
PciI ACATGT 3 cut(s) 234, 5064, 6585
PciSI GCTCTTC 5 cut(s) 1403, 2804, 4068, 5309, 6483
PcsI WCGNNNNNNNCGW 3 cut(s) 1255, 2638, 6668
PctI GAATGC 5 cut(s) 950, 5125, 5598, 5786, 6188
PdiI GCCGGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 767
PflMI CCANNNNNTGG 6 cut(s) 450, 1880, 1996, 5432, 6749, 6946
PfoI TCCNGGA 1 cut(s) 5149
Ppu21I YACGTR 1 cut(s) 3683
PpuMI RGGWCCY 1 cut(s) 3316
PscI ACATGT 3 cut(s) 234, 5064, 6585
PshAI GACNNNNGTC 3 cut(s) 2790, 4770, 6113
PsiI TTATAA 2 cut(s) 1560, 2262
Psp124BI GAGCTC 2 cut(s) 283, 5304
Psp1406I AACGTT 1 cut(s) 5023
Psp5II RGGWCCY 1 cut(s) 3316
Psp6I CCWGG 9 cut(s) 273, 407, 826, 1680, 1873, 2179, 4838, 5425, 6649
PspFI CCCAGC 3 cut(s) 1199, 3393, 6531
PspGI CCWGG 9 cut(s) 273, 407, 826, 1680, 1873, 2179, 4838, 5425, 6649
PspPPI RGGWCCY 1 cut(s) 3316
PspXI VCTCGAGB 1 cut(s) 587
PstI CTGCAG 3 cut(s) 1737, 2824, 5518
PstNI CAGNNNCTG 1 cut(s) 5432
PteI GCGCGC 1 cut(s) 163
PvuII CAGCTG 4 cut(s) 4081, 4654, 5440, 6106
SacI GAGCTC 2 cut(s) 283, 5304
SapI GCTCTTC 5 cut(s) 1403, 2804, 4068, 5309, 6483
ScaI AGTACT 2 cut(s) 4970, 6914
SexAI ACCWGGT 1 cut(s) 826
SfcI CTRYAG 7 cut(s) 1489, 1733, 2289, 2820, 5265, 5514, 5657
Sfr274I CTCGAG 2 cut(s) 317, 587
SgsI GGCGCGCC 1 cut(s) 163
SlaI CTCGAG 2 cut(s) 317, 587
SmaI CCCGGG 1 cut(s) 5033
SmlI CTYRAG 9 cut(s) 134, 317, 572, 587, 1237, 1571, 2735, 2936, 4445
SmoI CTYRAG 9 cut(s) 134, 317, 572, 587, 1237, 1571, 2735, 2936, 4445
SphI GCATGC 1 cut(s) 6869
SseBI AGGCCT 1 cut(s) 1387
SspI AATATT 2 cut(s) 1705, 2164
SspMI CTAG 4 cut(s) 1971, 4917, 6746, 6962
SstI GAGCTC 2 cut(s) 283, 5304
StuI AGGCCT 1 cut(s) 1387
StyI CCWWGG 8 cut(s) 804, 977, 1392, 2168, 4112, 6805, 6836, 6906
TaiI ACGT 8 cut(s) 2644, 2676, 2794, 2989, 3685, 4983, 5026, 6138
TaqII GACCGA 4 cut(s) 909, 1299, 1410, 5877
TatI WGTACW 9 cut(s) 306, 939, 1737, 2697, 3115, 3826, 4648, 4968, 6912
TauI GCSGC 2 cut(s) 1801, 2552
TseFI GTSAC 8 cut(s) 81, 2642, 3983, 4004, 4039, 5578, 6115, 6872
Tsp45I GTSAC 8 cut(s) 81, 2642, 3983, 4004, 4039, 5578, 6115, 6872
TspGWI ACGGA 8 cut(s) 69, 338, 1682, 2647, 3803, 4712, 4979, 5391
TspMI CCCGGG 1 cut(s) 5031
Van91I CCANNNNNTGG 6 cut(s) 450, 1880, 1996, 5432, 6749, 6946
VneI GTGCAC 1 cut(s) 4725
XagI CCTNNNNNAGG 1 cut(s) 4772
XcmI CCANNNNNNNNNTGG 2 cut(s) 4370, 5889
XhoI CTCGAG 2 cut(s) 317, 587
XmaI CCCGGG 1 cut(s) 5031
XmiI GTMKAC 4 cut(s) 270, 823, 3749, 6813
XmnI GAANNNNTTC 1 cut(s) 767
XspI CTAG 4 cut(s) 1971, 4917, 6746, 6962
ZrmI AGTACT 2 cut(s) 4970, 6914
Zsp2I ATGCAT 5 cut(s) 1023, 2755, 4680, 6626, 6871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.