Rroxscaffold_2G00089540

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000798
Physical Location & Seq
Reverse (-)
11499937 .. 11506949
7013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089540.1

Sequence Viewer

Length: 6942 bp
ATGTGTAACAACGCCGAAATTTCACCGCCGGGGACGGGAGATTCCTCGATTCCATCGGCGGTGGCTCAGACCTGTCTTCCGTCACCGGCCGATCTTGAAGAGAAAGCCCGTAGATGGCAGCAGCTTTCCTCCCGCAAGTGGTCCAATCGCCACGGCGAACGGCGCGTCGTCGATTCACAAAAACAGGACATGCCTGCGGAGCATGTGAGGAAGATCGTGAAAGAGCACGGCGACATGTCATCGAAGAAGTACCGGCAAGACAAGCGGGTCTACCTGGGAGCTCTCAAGTTCGTTCCTCACGCCGTGTACAAGCTCCTCGAGAATATGCCGATGCCGTGGGAGCAGGTCCCCGATCTACATGGCCCAATGGGCCACAATGTGGATCATGATGAGGAGGGGAAGAAGAGGGATCGCCGGCATTTCAAAAGAATGAGGTTTCCGCCGTTTGATGACGAAGAGCCTGTGTTGGATTATGCAGATAACTTGTTGGATGTTGAGCCTCTTGAGCCGATTCAGCTGGAGCTTGATGCCGAAGAGGATTCCGCTGTGTATAAGTGGTTTTATGACCATAAACCTCTAATGAAGACAAAGCTGATCAACGGCCCGACCTACTGGAAGTGGCAGCTCTCGCTGCCGATTATGGAAGCCCTTTACCGGCTTTCAGGACAGGTAATTTCCGATTTGAGTGATCGGAACTATTTCTATTTGTTTGATAAGGAGTCGTTTCTGACAGCCAAGGCATTGAATATGTGTATGCCAGGTGGGCCGAAATTCGAGCCATTGTTTAGGGATATGGAGCAAGGGGAGGAGGATTGGAATGAGTTTAATGACATAAACAAGCTCATTATTCGGTCTCCGCTGAGATCAGAGTACAAAATTGCATTCCCTAATCTTTACAACAACAGGCCGAGGAAGGTAAAGCTCTCTGTTTATCACACACCAATGGTTATGCATATTAAAACTGAGGATCCTGACCTGCCTGCATTTTATTATGATCCATTGCTAAACCCAATACCAGGCACTAACAGGAGTAGTTATGATAATAGTGAGGAAGATGGTGATTTTGTTTTGCCTGAAGGGGTGGAGCCTCTTTTGAGTCATACTCAGCTTTATACTGACACCACTGCTGCTGGGATTTCCCTGTATTTTGCCCCACGCCCTTTTAACTTGAGGTCTGGTCGGACTTGTCGCGCAGAGGATATACCTCTTGTGTCAGAGTGGTTTAAGGAGCATTGTCCTCGGTCATATCCTGTTAAGGTTCGAGTCAGCTATCAGAAGTTGTTGAAATCGTTTGTGTTGAATGAGCTGCATCGTAGGCCTCCCAAGGCACAAAAGAAGAAGGACTTGTTTCGGTCTCTTCAGGCTACCAAGTTTTTCCAAACTACTGAGCTTGATTGGGTTGAAGCGGGTCTTCAAATCTGTAGGCAGGGTCATAACATGCTAAATCTGCTGATACATAGGAAAGGTCTGAACTATCTTCACCTTGATTATAATTTTAATCTCAAGCCTGTGAAAACCCTGACAACAAAAGAGCGTAAGAAATCTCGGTTTGGTAATTCTTTTCATCTGTTGCGTGAAATCTTGCGGTTAACAAAGCTTGTAGTTGATGCCCAGGTCCAGTTCCGTTTGGGTAATATCGATGCCTTTCAATTGGCTGATGGTCTGCAGTACATATTTTCTCATGTTGGTCAGTTAACTGGAATGTACCGTTACAAGTATAGGTTGATGCGGCAGATTAGAATGAGCAAAGATTTGAAGCACTTGATTTACTACCGTTTCAATACTGGGCCAGTCGGGAAAGGGCAGGGATGTGGATTCTGGGCACCAATGTGGAGGGTGTGGTTGTCCTTCCTTCGTGGCATGGTCCCTCTTCTGGAACAGTGGTTGGGAAATTTACTTGCTAGACAATTTGAAGGGCGCCATTCTGTGGCAAAGACAGTAACTAAGCAGCGTGTCGAAAGCAATTTCGACTTGGAGTTTCGAGCAGATGTCTTGAAAGATATTCTGGATGCCATGCCAGTGGGCATGAAGCAAAACAAGGCGAAAACAATTATGCAGCATCTCAGTGAGGCATGGCGTTGTTGGAAAGCAAATATTCCTTGGAAGGTTCCTGGTATGCCTGCTCCGGTTGAGAATATAATTCTTCGTTATGTCAAGTCAAAGGCAGATTGGTGGACACATGGTGCTCATTATAACCGTGAACGTATTAGAAGAGGTGCTACAGTGGATAAGACAGTTTGTCGAAAGAATCTTGGAAGATTAACTCGCCTTTGGCTAAAGGGAGAGCAGGAGCGACAGCACAATTATCTCAAAGATGGTCCATATATTTCACCAGAAGAAGCAATAGCAATTTACTCAACAACTGTGCATTGGTTGGAGTCAAGAAATTTCCAACATATTCCATTTCCCCCTTTGTCATATAAGCATGATACCAAGCTACTTATCCTTGCTCTGGAGAGGTTGAAAGAATCTTATACTGCGGCAGGAAAATTGAACCAGCATCAAAGAGAGGAGTTGGGTCTCATTGAACAAGCCTATGATAACCCCCATGAGGCATTGATACGGATAAAGCGTCACTTGATCTCACAGCGTGCCTTCAAAGACGTGAGCATAGAGTTCATGGATCTGTACAGCCATCATATTCCAGTTTTTGAGATTGACCCTCTTGAGAAGATTACAGATGCATATCTTGATCAATATCTATGGTATGAGGCTGACAAACGTCAGCTCTTCCCGAACTGGATCAAGCCTGCAGACTCAGAGCCACCTCCACTTTTGGTCTATAAATGGTGTCAGGGGATAAACAATTTAGAGAGTATATGGGACACAAGTGAGGGGGAGTGTGTTGTGATGCTTCAGACAAAACTTGAGAGATTATCTGAGAAGATTGACTTGACATTGCTAAATAGGCTTCTACGTCTTGTTCTTGACCATAACATTGCTGATTATATCACTGCAAAAAACAATGTGGTATTGTCTTACAAAGATATGAGTCATACAAATTCATATGGTCTTATACGGGGTCTCCAGCCTGCATCATTTGTTGTACAGTATTATGGGCTTGTTTTGGATCTAATGCTTCTTGGATTGACTCGGGCCAATGAAATTGCAGGTCCACAGCATAAGCCAAATGAGTTCATGACTTATGGGGCCACAAAGGTTGAGACTCGCCATCCTATCAGGCTGTACTCTCGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACTCAGGAGGAGGCTCGGGACCTCATACAGCGATATCTTACCGAGCATCCAGATCCCAATAATGAGAATATGGTTGGATATAATAATAAGAAGTGCTGGGCAAGAGATGCAAGGATGAGGCTCCTGAAACATGATGTCAATCTTGGAAGAAGTGTCTTTTGGAATGTCAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAATGGGAGGACAGCTTTGTTTCTGTTTACAGCAAGGATAATCCAAATTTGCTTTTCAGCATGTGTGGATTTGAAGTAAGAATACTTCCCAAGATAAGAATGACTACGAAGGAACACCAAAGAGATGGAGTTTGGAACTTGCAAAATGAACAGACAAAGGAAAGGACTGCTGTTGCTTTTTTACGTGTTGAGGATGAATACATGAAGGCGTTTGAGAATCGTGTAAGGCAGATCCTTATGTCCTCAGGGTCTACAACATTCACAAAAGTTATCAACAAATGGAATACAGCCCTTATTGGTCTTATGACTTACTTCCGTGAAGCAGTTGTACATACAGCGGAGCTGTTAGATTTACTGGTCAAATGTGAAAATAAAATTCAGACCCGCATTAAGATTGGATTGAATTCAAAGATGCCAAGCAGGTTCCCACCTGTCATTTTTTACACGCCAAAAGAAATCGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAGAGTGACCTCAGATACTCTCGGCAGACAGAAATTGGTGTGACACACTTGAGGAGTGGAATGAGTCATGAAGAAGAGCAGCTGATTCCAAATCTTTACCGTTACATACAACCATGGGAGAGTGAGTTTATAGATTCACAGCGTGTTTGGGCAGAATATGCACTAAAAAGACAGGAAGCTATGGAACAGAATCGGCGTCTTACCCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACATTGTTCCAGAAGGATCAACACACTTTAGCATATGACAAAGGGTGGAGAGTTCGCACAGATTTTAAGCAGTACCAGATCTTGAAGCAAAATCCTTTCTGGTGGACACACCAAAGGCATGATGGAAAATTGTGGAATTTGAATAATTATAGAACTGATGTGATCCAAGCTCTTGGAGGAGTTGAGGGAATACTTGAGCACACCTTATTCAAAGGAACATACTTCCCTACTTGGGAGGGTCTCTTCTGGGAGAAGGCATCTGGATTTGAGGAATCGATGAAGTATAAAAAGTTGACTAATGCCCAAAGATCTGGCCTCAACCAAATCCCCAATCGTAGGTTTACGCTTTGGTGGTCGCCTACCATAAATCGAGCCAATGTATATGTCGGTTTTCAAGTACAGCTGGATCTGACAGGAATCTTTATGCATGGGAAGATCCCAACCTTGAAGATATCATTGATTCAGATATTTCGTTCACACTTGTGGCAGAAGATTCATGAAAGTGTTGTGATGGATCTTTGTCAGGTTTTGGATCAGGAGTTGGATGCTTTGGAAATTGAGACTGTGCAGAAGGAAACCATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCAGACATTCTCCTCTTTTCTGCTCATAAATGGCAAATGTCGAAACCTAGTCTTGTGGCTGAGCCGAAGGATGAGTATGATAAAAAAACAAGCAACAAGTACTGGATAGACGTGCAGCTCCGTTGGGGGGATTATGATTCTCATGATATTGAACGTTACACCTGGGCAAAGTTTATGGACTATACAACTGATAACATGTCTATCTATCCTTCTCTGACGGGAGTGATGATTGGTATTGATTTAGCCTATAATGTGCATTCTGCATTTGGTAACTGGTTTCCTGGATCAAAACCACTGATTCAACAGGCAATGAACAAGATCATGAAGTCAAATCCGGCTTTATATGTGTTGAGGGAGCGCATAAGGAAAGGATTGCAGTTGTATTCCTCTGAGCCTACAGAGCCATATTTGTCTTCCCAAAACTATGGGGAGCTCTTCAGCAATCAAATTAAATGGTTTGTTGACGATACAAATGTGTATCGTGTTACAATGCACAAGACTTTTGACGGAAATCTTGTTACAAAACCCATCAATGGTGCTATCTTCATGTTCAATCCAGGGACTGGGCAGCTGTTCTTGAAGGTTATCCACACAAGTGTGTGGGCAGGACAGAAGCGACTTGGGCAGTTGGCTAAATGGAAAGCTGCAGAAGAAGTTGCTGCTCTTGTGCGTTCTCTACCTGTTGAAGAACAGCCAAAGCAAATTATTGTGACTCGTAAGGGAATGCTCGACCCATTGGAGGTTCATTTGCTGGATTTTCCCAACATAATCATAAAAGGAAGTGAGCTACAGCTTCCTTTTCAGGTTTGCTTGAAAATTGAGAAGTTGAATGATCTGGTACTAAAGGCTACTGAGCCACAGATGGTTCTTTTTAATGTTTACGATGATTGGTTGAAGAGTGTTTCATCATACACTGCATTCTCTCGGCTCATTCTGATTCTTCGGGCACTACATGTGAACAGAGACAAGGCAAGTATGTTGTTGAAGCCTGACCGAACAGTCATTAGTGAGCCACACCACATTTGGCCTTCTCTTTCTGATGAGCAATGGATGAAGGTTGAGCGTGCTCTAACAGATCTCATATTGTCAGACTCTGCGAAAAAGAATAATGTGAGTACTTCAGCCCTCACACAGTCTGAAATTCGTGATATAATACTTGGAGCAGAGATTACTCCACCTTCTCAACAGAGGCAAGAGATAGCAGAGATTGAGAAACAGGCTAAAGGAGTTAGTCAGCTGACAGCAGTCACCACGAGGACCACAAACGTGCATGGAGATGAGCTTATTTCCACCACTACAAGTCAATATGAGCAAGCTGCATTCAGGTCCAAAACTGATTGGCGTGTGAGAGCAATATCAGCTACAAATCTACATCTTCGTGTCAACCATATTTATGTGAGTTCAGAAGATACAAAGGAAACAGGCTACACTTACATCATGCCAATGAACATTTTGAAAAAGTTTATCTGTGTTGCTGATCTGAGGACACAAGTTGCTGGTTACTTGTATGGTATAAGCCCACCTGACAACCCTCAGGTTAAGGAAATTCGCTGCATTGTGATGCCACCACAGCAGGGCACCCACCAGCAAGTTCATCTTCCATCAGCTCTTCCTGAGCATGATTTCCTCAATTATTTGGAACCTCTGGGATGGATGCACACCCAGCCAAATGAGCTCCCTCAGCTTTCTCCTCAGGATCTCACCACTCATGCTAACATGTTGGAGAATGGCAAGCAATGGGATAGGGAGAAATGCATTATTATGACCTGCGCTTTTACTCCAGGTTCGTGCTCGTTAACTGCATATAAACTTACTCCATCTGGTTATGAGTGGGGATGTTGTTCTAATAAGAACAGCAGAAGAAACAATAATCCACTAGATGGATACCTTCCAACTCATTATGAGAAGGTCCAGATGCTTCTCAGTGATCGCTTCCTTGGTTTCTACATGGTTCCTGATAGTTGTCCATGGAACTTCAATTTCATGGGAGTGAGGCATGCATGTGACATGAAGTATGGCATCAAGCTTGAACCACCCAAGGAGTACTATCACGAGGAACACCGACCAACCCATTTCATGGAGTTCAGCGAACTAGATAGGGATGACCCAATGGAGTTAGATCGAGATTATGCCTACGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

2313

Amino Acids

269.93

Weight (kDa)

8.73

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 61 - 117 4.6e-27 PRO8NT (NUC069), PrP8 N-terminal domain
PRO8NT PF08082 134 - 189 5.9e-22 PRO8NT (NUC069), PrP8 N-terminal domain
PROCN PF08083 373 - 776 3.9e-225 PROCN (NUC071) domain
RRM_4 PF10598 961 - 1050 1.7e-44 RNA recognition motif of the spliceosomal PrP8
U5_2-snRNA_bdg PF10597 1211 - 1346 8.7e-74 U5-snRNA binding site 2 of PrP8
U6-snRNA_bdg PF10596 1416 - 1574 1.9e-88 U6-snRNA interacting domain of PrP8
PRP8_domainIV PF12134 1734 - 1963 1.8e-125 PRP8 domain IV core
JAB PF01398 2076 - 2172 9.6e-11 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
PROCT PF08084 2186 - 2308 8e-43 PROCT (NUC072) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1491, 2193
AasI GACNNNNNNGTC 2 cut(s) 266, 5798
Acc36I ACCTGC 5 cut(s) 334, 984, 3101, 3840, 6575
AccB1I GGYRCC 3 cut(s) 1822, 1917, 6377
AccB7I CCANNNNNTGG 5 cut(s) 379, 1927, 5363, 6680, 6877
AccI GTMKAC 2 cut(s) 270, 3680
AccII CGCG 2 cut(s) 165, 1191
AclI AACGTT 1 cut(s) 4954
AcoI YGGCCR 1 cut(s) 87
AcuI CTGAAG 5 cut(s) 1095, 1343, 2840, 5221, 5904
AcyI GRCGYC 2 cut(s) 1918, 4126
AdeI CACNNNGTG 6 cut(s) 304, 379, 2183, 2591, 3935, 4073
AflIII ACRYGT 5 cut(s) 234, 3613, 4995, 5752, 6516
AhdI GACNNNNNGTC 1 cut(s) 2238
AjiI CACGTC 2 cut(s) 2605, 4912
AjuI GAANNNNNNNTTGG 2 cut(s) 3370, 3402
AleI CACNNNNGTG 5 cut(s) 1828, 4663, 5394, 5396, 6065
Alw21I GWGCWC 8 cut(s) 228, 283, 2188, 4383, 5235, 5869, 6477, 6593
Alw26I GTCTC 9 cut(s) 858, 1359, 2525, 3029, 3158, 3410, 4427, 4736, 5757
AlwNI CAGNNNCTG 2 cut(s) 5363, 5894
Ama87I CYCGRG 3 cut(s) 317, 3093, 3243
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
Asp700I GAANNNNTTC 1 cut(s) 698
AspLEI GCGC 5 cut(s) 165, 1193, 1920, 5160, 6572
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 7 cut(s) 15, 75, 1070, 1472, 2322, 6040, 6493
AvaI CYCGRG 3 cut(s) 317, 3093, 3243
AxyI CCTNAGG 3 cut(s) 3673, 6333, 6492
BaeGI GKGCMC 3 cut(s) 1825, 5749, 6380
BaeI ACNNNNGTAYC 2 cut(s) 5259, 5292
BamHI GGATCC 1 cut(s) 967
BanI GGYRCC 3 cut(s) 1822, 1917, 6377
BanII GRGCYC 3 cut(s) 283, 5235, 6477
BarI GAAGNNNNNNTAC 8 cut(s) 2203, 2235, 3495, 3498, 3527, 3530, 3741, 3773
BauI CACGAG 2 cut(s) 6052, 6851
BbsI GAAGAC 4 cut(s) 68, 590, 1403, 5205
Bbv12I GWGCWC 8 cut(s) 228, 283, 2188, 4383, 5235, 5869, 6477, 6593
BbvCI CCTCAGC 1 cut(s) 6480
BceAI ACGGC 7 cut(s) 169, 176, 244, 287, 319, 427, 616
BcgI CGANNNNNNTGC 2 cut(s) 1220, 1254
BciVI GTATCC 1 cut(s) 6677
BclI TGATCA 2 cut(s) 594, 2692
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 9 cut(s) 858, 1359, 2525, 3029, 3158, 3410, 4427, 4736, 5757
BfaI CTAG 4 cut(s) 1902, 4848, 6677, 6893
BfmI CTRYAG 7 cut(s) 1420, 1664, 2220, 2751, 5196, 5445, 5588
BfoI RGCGCY 1 cut(s) 1921
BfuAI ACCTGC 5 cut(s) 334, 984, 3101, 3840, 6575
BfuI GTATCC 1 cut(s) 6677
BglI GCCNNNNNGGC 2 cut(s) 369, 763
BglII AGATCT 3 cut(s) 4260, 4490, 5875
BlpI GCTNAGC 1 cut(s) 4860
BmcAI AGTACT 3 cut(s) 4901, 5917, 6845
BmeRI GACNNNNNGTC 1 cut(s) 2238
BmeT110I CYCGRG 3 cut(s) 317, 3093, 3243
BmgBI CACGTC 2 cut(s) 2605, 4912
BmrI ACTGGG 2 cut(s) 1794, 5373
BmuI ACTGGG 2 cut(s) 1794, 5373
BoxI GACNNNNGTC 2 cut(s) 2721, 6044
BpiI GAAGAC 4 cut(s) 68, 590, 1403, 5205
BplI GAGNNNNNCTC 2 cut(s) 1087, 1119
BpmI CTGGAG 4 cut(s) 539, 2474, 3011, 6564
Bpu10I CCTNAGC 2 cut(s) 6414, 6480
Bpu1102I GCTNAGC 1 cut(s) 4860
BpuEI CTTGAG 8 cut(s) 269, 524, 1189, 1487, 2687, 2888, 4000, 4397
BpuMI CCSGG 1 cut(s) 30
Bsa29I ATCGAT 2 cut(s) 1638, 4457
BsaAI YACGTR 1 cut(s) 3614
BsaBI GATNNNNATC 3 cut(s) 2685, 2697, 3366
BsaHI GRCGYC 2 cut(s) 1918, 4126
BsaI GGTCTC 5 cut(s) 858, 1359, 2525, 3029, 4427
BsaWI WCCGGW 1 cut(s) 2125
BsaXI ACNNNNNCTCC 4 cut(s) 3009, 3039, 4040, 4070
Bse118I RCCGGY 4 cut(s) 85, 252, 414, 654
Bse21I CCTNAGG 3 cut(s) 3673, 6333, 6492
Bse3DI GCAATG 6 cut(s) 998, 2897, 2937, 5115, 5852, 6542
Bse8I GATNNNNATC 3 cut(s) 2685, 2697, 3366
BseCI ATCGAT 2 cut(s) 1638, 4457
BseJI GATNNNNATC 3 cut(s) 2685, 2697, 3366
BseMI GCAATG 6 cut(s) 998, 2897, 2937, 5115, 5852, 6542
BseRI GAGGAG 9 cut(s) 305, 407, 821, 2525, 3251, 3997, 4374, 4802, 6480
BseSI GKGCMC 3 cut(s) 1825, 5749, 6380
BseX3I CGGCCG 1 cut(s) 87
BseYI CCCAGC 3 cut(s) 1130, 3324, 6462
BsgI GTGCAG 3 cut(s) 4769, 4821, 4934
Bsh1236I CGCG 2 cut(s) 165, 1191
Bsh1285I CGRYCG 1 cut(s) 90
BshNI GGYRCC 3 cut(s) 1822, 1917, 6377
BshVI ATCGAT 2 cut(s) 1638, 4457
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 8 cut(s) 228, 283, 2188, 4383, 5235, 5869, 6477, 6593
BsiHKCI CYCGRG 3 cut(s) 317, 3093, 3243
BsiSI CCGG 7 cut(s) 29, 86, 253, 415, 655, 2126, 5135
BslFI GGGAC 6 cut(s) 46, 332, 1850, 2837, 3260, 5374
BsmAI GTCTC 9 cut(s) 858, 1359, 2525, 3029, 3158, 3410, 4427, 4736, 5757
BsmBI CGTCTC 1 cut(s) 3410
BsmFI GGGAC 6 cut(s) 46, 332, 1850, 2837, 3260, 5374
BsmI GAATGC 5 cut(s) 881, 5056, 5529, 5717, 6119
Bso31I GGTCTC 5 cut(s) 858, 1359, 2525, 3029, 4427
BsoBI CYCGRG 3 cut(s) 317, 3093, 3243
Bsp1407I TGTACA 4 cut(s) 306, 2628, 3046, 3757
Bsp1720I GCTNAGC 1 cut(s) 4860
Bsp19I CCATGG 2 cut(s) 4043, 6767
BspDI ATCGAT 2 cut(s) 1638, 4457
BspFNI CGCG 2 cut(s) 165, 1191
BspHI TCATGA 6 cut(s) 385, 3138, 3997, 4678, 4942, 5121
BspMAI CTGCAG 3 cut(s) 1668, 2755, 5449
BspMI ACCTGC 5 cut(s) 334, 984, 3101, 3840, 6575
BspQI GCTCTTC 5 cut(s) 450, 2735, 3999, 5240, 6414
BspT107I GGYRCC 3 cut(s) 1822, 1917, 6377
BspTNI GGTCTC 5 cut(s) 858, 1359, 2525, 3029, 4427
BsrDI GCAATG 6 cut(s) 998, 2897, 2937, 5115, 5852, 6542
BsrFI RCCGGY 4 cut(s) 85, 252, 414, 654
BsrGI TGTACA 4 cut(s) 306, 2628, 3046, 3757
BssAI RCCGGY 4 cut(s) 85, 252, 414, 654
BssNI GRCGYC 2 cut(s) 1918, 4126
BssSI CACGAG 2 cut(s) 6052, 6851
BssT1I CCWWGG 7 cut(s) 735, 1323, 2099, 4043, 6736, 6767, 6837
Bst2BI CACGAG 2 cut(s) 6052, 6851
BstACI GRCGYC 2 cut(s) 1918, 4126
BstAPI GCANNNNNTGC 2 cut(s) 3335, 4088
BstAUI TGTACA 4 cut(s) 306, 2628, 3046, 3757
BstBAI YACGTR 1 cut(s) 3614
BstDSI CCRYGG 4 cut(s) 151, 335, 4043, 6767
BstFNI CGCG 2 cut(s) 165, 1191
BstH2I RGCGCY 1 cut(s) 1921
BstHHI GCGC 5 cut(s) 165, 1193, 1920, 5160, 6572
BstMAI GTCTC 9 cut(s) 858, 1359, 2525, 3029, 3158, 3410, 4427, 4736, 5757
BstMCI CGRYCG 1 cut(s) 90
BstPAI GACNNNNGTC 2 cut(s) 2721, 6044
BstSFI CTRYAG 7 cut(s) 1420, 1664, 2220, 2751, 5196, 5445, 5588
BstSLI GKGCMC 3 cut(s) 1825, 5749, 6380
BstUI CGCG 2 cut(s) 165, 1191
BstV2I GAAGAC 4 cut(s) 68, 590, 1403, 5205
BstXI CCANNNNNNTGG 5 cut(s) 2020, 3554, 4355, 4493, 5225
BstZI CGGCCG 1 cut(s) 87
Bsu15I ATCGAT 2 cut(s) 1638, 4457
Bsu36I CCTNAGG 3 cut(s) 3673, 6333, 6492
BsuI GTATCC 1 cut(s) 6677
BsuTUI ATCGAT 2 cut(s) 1638, 4457
BtgI CCRYGG 4 cut(s) 151, 335, 4043, 6767
BtrI CACGTC 2 cut(s) 2605, 4912
BtsI GCAGTG 3 cut(s) 1122, 2952, 5712
BtsIMutI CAGTG 9 cut(s) 1122, 1886, 2025, 2071, 2229, 2952, 5093, 5712, 6730
BveI ACCTGC 5 cut(s) 334, 984, 3101, 3840, 6575
CaiI CAGNNNCTG 2 cut(s) 5363, 5894
CciI TCATGA 6 cut(s) 385, 3138, 3997, 4678, 4942, 5121
CfoI GCGC 5 cut(s) 165, 1193, 1920, 5160, 6572
Cfr10I RCCGGY 4 cut(s) 85, 252, 414, 654
ClaI ATCGAT 2 cut(s) 1638, 4457
CseI GACGC 3 cut(s) 154, 2561, 4115
CspCI CAANNNNNGTGG 2 cut(s) 3142, 3177
DinI GGCGCC 1 cut(s) 1919
DraIII CACNNNGTG 6 cut(s) 304, 379, 2183, 2591, 3935, 4073
DrdI GACNNNNNNGTC 2 cut(s) 266, 5798
DriI GACNNNNNGTC 1 cut(s) 2238
DseDI GACNNNNNNGTC 2 cut(s) 266, 5798
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
Eam1105I GACNNNNNGTC 1 cut(s) 2238
EciI GGCGGA 1 cut(s) 429
Ecl136II GAGCTC 3 cut(s) 281, 5233, 6475
EclXI CGGCCG 1 cut(s) 87
Eco130I CCWWGG 7 cut(s) 735, 1323, 2099, 4043, 6736, 6767, 6837
Eco147I AGGCCT 1 cut(s) 1318
Eco24I GRGCYC 3 cut(s) 283, 5235, 6477
Eco31I GGTCTC 5 cut(s) 858, 1359, 2525, 3029, 4427
Eco32I GATATC 2 cut(s) 3263, 4635
Eco52I CGGCCG 1 cut(s) 87
Eco53kI GAGCTC 3 cut(s) 281, 5233, 6475
Eco57I CTGAAG 5 cut(s) 1095, 1343, 2840, 5221, 5904
Eco81I CCTNAGG 3 cut(s) 3673, 6333, 6492
Eco88I CYCGRG 3 cut(s) 317, 3093, 3243
EcoICRI GAGCTC 3 cut(s) 281, 5233, 6475
EcoO109I RGGNCCY 2 cut(s) 346, 3247
EcoRI GAATTC 1 cut(s) 3832
EcoRV GATATC 2 cut(s) 3263, 4635
EcoT14I CCWWGG 7 cut(s) 735, 1323, 2099, 4043, 6736, 6767, 6837
EcoT22I ATGCAT 5 cut(s) 954, 2686, 4611, 6557, 6802
EcoT38I GRGCYC 3 cut(s) 283, 5235, 6477
EgeI GGCGCC 1 cut(s) 1919
EheI GGCGCC 1 cut(s) 1919
ErhI CCWWGG 7 cut(s) 735, 1323, 2099, 4043, 6736, 6767, 6837
Esp3I CGTCTC 1 cut(s) 3410
FaqI GGGAC 6 cut(s) 46, 332, 1850, 2837, 3260, 5374
FauI CCCGC 4 cut(s) 140, 258, 1399, 3821
FauNDI CATATG 2 cut(s) 3007, 4216
FbaI TGATCA 2 cut(s) 594, 2692
FblI GTMKAC 2 cut(s) 270, 3680
FriOI GRGCYC 3 cut(s) 283, 5235, 6477
FspBI CTAG 4 cut(s) 1902, 4848, 6677, 6893
GlaI GCGC 5 cut(s) 164, 1192, 1919, 5159, 6571
GsaI CCCAGC 3 cut(s) 1134, 3328, 6466
GsuI CTGGAG 4 cut(s) 539, 2474, 3011, 6564
HaeII RGCGCY 1 cut(s) 1921
HapII CCGG 7 cut(s) 29, 86, 253, 415, 655, 2126, 5135
HgaI GACGC 3 cut(s) 154, 2561, 4115
HhaI GCGC 5 cut(s) 165, 1193, 1920, 5160, 6572
Hin1I GRCGYC 2 cut(s) 1918, 4126
Hin6I GCGC 5 cut(s) 163, 1191, 1918, 5158, 6570
HinP1I GCGC 5 cut(s) 163, 1191, 1918, 5158, 6570
HincII GTYRAC 6 cut(s) 1590, 1695, 4476, 5263, 6184, 6597
HindII GTYRAC 6 cut(s) 1590, 1695, 4476, 5263, 6184, 6597
HindIII AAGCTT 2 cut(s) 1595, 6824
HpaI GTTAAC 3 cut(s) 1590, 1695, 6597
HpaII CCGG 7 cut(s) 29, 86, 253, 415, 655, 2126, 5135
HphI GGTGA 7 cut(s) 15, 75, 1070, 1472, 2322, 6040, 6493
Hpy99I CGWCG 2 cut(s) 170, 173
HpyCH4IV ACGT 8 cut(s) 2203, 2604, 2722, 2917, 3613, 4911, 4954, 6066
HpySE526I ACGT 8 cut(s) 2203, 2604, 2722, 2917, 3613, 4911, 4954, 6066
Hsp92I GRCGYC 2 cut(s) 1918, 4126
HspAI GCGC 5 cut(s) 163, 1191, 1918, 5158, 6570
KasI GGCGCC 1 cut(s) 1917
KroI GCCGGC 1 cut(s) 414
KroNI GCCGGC 1 cut(s) 416
Ksp22I TGATCA 2 cut(s) 594, 2692
KspAI GTTAAC 3 cut(s) 1590, 1695, 6597
LguI GCTCTTC 5 cut(s) 450, 2735, 3999, 5240, 6414
MaeI CTAG 4 cut(s) 1902, 4848, 6677, 6893
MaeII ACGT 8 cut(s) 2203, 2604, 2722, 2917, 3613, 4911, 4954, 6066
MfeI CAATTG 1 cut(s) 1649
Mly113I GGCGCC 1 cut(s) 1918
Mph1103I ATGCAT 5 cut(s) 954, 2686, 4611, 6557, 6802
MroNI GCCGGC 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 698
MspA1I CMGCKG 8 cut(s) 517, 545, 859, 3767, 4012, 4585, 5371, 6037
MspI CCGG 7 cut(s) 29, 86, 253, 415, 655, 2126, 5135
MunI CAATTG 1 cut(s) 1649
Mva1269I GAATGC 5 cut(s) 881, 5056, 5529, 5717, 6119
MvnI CGCG 2 cut(s) 165, 1191
NaeI GCCGGC 1 cut(s) 416
NarI GGCGCC 1 cut(s) 1918
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 2 cut(s) 4043, 6767
NdeI CATATG 2 cut(s) 3007, 4216
NgoMIV GCCGGC 1 cut(s) 414
NmeAIII GCCGAG 3 cut(s) 933, 3931, 5704
NmuCI GTSAC 8 cut(s) 81, 2573, 3914, 3935, 3970, 5509, 6046, 6803
NsiI ATGCAT 5 cut(s) 954, 2686, 4611, 6557, 6802
OliI CACNNNNGTG 5 cut(s) 1828, 4663, 5394, 5396, 6065
PaeI GCATGC 1 cut(s) 6800
PaeR7I CTCGAG 1 cut(s) 317
PagI TCATGA 6 cut(s) 385, 3138, 3997, 4678, 4942, 5121
PceI AGGCCT 1 cut(s) 1318
PciI ACATGT 4 cut(s) 234, 4995, 5752, 6516
PciSI GCTCTTC 5 cut(s) 450, 2735, 3999, 5240, 6414
PcsI WCGNNNNNNNCGW 1 cut(s) 2569
PctI GAATGC 5 cut(s) 881, 5056, 5529, 5717, 6119
PdiI GCCGGC 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 698
PflMI CCANNNNNTGG 5 cut(s) 379, 1927, 5363, 6680, 6877
PfoI TCCNGGA 1 cut(s) 5080
PluTI GGCGCC 1 cut(s) 1921
Ppu21I YACGTR 1 cut(s) 3614
PpuMI RGGWCCY 2 cut(s) 346, 3247
PscI ACATGT 4 cut(s) 234, 4995, 5752, 6516
PshAI GACNNNNGTC 2 cut(s) 2721, 6044
PsiI TTATAA 2 cut(s) 1491, 2193
Psp124BI GAGCTC 3 cut(s) 283, 5235, 6477
Psp1406I AACGTT 1 cut(s) 4954
Psp5II RGGWCCY 2 cut(s) 346, 3247
PspFI CCCAGC 3 cut(s) 1130, 3324, 6462
PspPPI RGGWCCY 2 cut(s) 346, 3247
PstI CTGCAG 3 cut(s) 1668, 2755, 5449
PstNI CAGNNNCTG 2 cut(s) 5363, 5894
PvuII CAGCTG 5 cut(s) 517, 4012, 4585, 5371, 6037
SacI GAGCTC 3 cut(s) 283, 5235, 6477
SapI GCTCTTC 5 cut(s) 450, 2735, 3999, 5240, 6414
ScaI AGTACT 3 cut(s) 4901, 5917, 6845
SfcI CTRYAG 7 cut(s) 1420, 1664, 2220, 2751, 5196, 5445, 5588
SfiI GGCCNNNNNGGCC 1 cut(s) 369
SfoI GGCGCC 1 cut(s) 1919
Sfr274I CTCGAG 1 cut(s) 317
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 9 cut(s) 284, 317, 503, 1168, 1502, 2666, 2867, 3979, 4376
SmoI CTYRAG 9 cut(s) 284, 317, 503, 1168, 1502, 2666, 2867, 3979, 4376
SphI GCATGC 1 cut(s) 6800
SseBI AGGCCT 1 cut(s) 1318
SspDI GGCGCC 1 cut(s) 1917
SspI AATATT 1 cut(s) 2095
SspMI CTAG 4 cut(s) 1902, 4848, 6677, 6893
SstI GAGCTC 3 cut(s) 283, 5235, 6477
StuI AGGCCT 1 cut(s) 1318
StyI CCWWGG 7 cut(s) 735, 1323, 2099, 4043, 6736, 6767, 6837
TaiI ACGT 8 cut(s) 2206, 2607, 2725, 2920, 3616, 4914, 4957, 6069
TaqII GACCGA 4 cut(s) 840, 1230, 1341, 5808
TauI GCSGC 2 cut(s) 1732, 2483
TscAI CASTG 9 cut(s) 1129, 1886, 2025, 2071, 2229, 2959, 5100, 5719, 6730
TseFI GTSAC 8 cut(s) 81, 2573, 3914, 3935, 3970, 5509, 6046, 6803
Tsp45I GTSAC 8 cut(s) 81, 2573, 3914, 3935, 3970, 5509, 6046, 6803
TspGWI ACGGA 6 cut(s) 69, 1613, 2578, 3734, 4910, 5322
TspRI CASTG 9 cut(s) 1129, 1886, 2025, 2071, 2229, 2959, 5100, 5719, 6730
Van91I CCANNNNNTGG 5 cut(s) 379, 1927, 5363, 6680, 6877
XcmI CCANNNNNNNNNTGG 2 cut(s) 4301, 5820
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 2 cut(s) 270, 3680
XmnI GAANNNNTTC 1 cut(s) 698
XspI CTAG 4 cut(s) 1902, 4848, 6677, 6893
ZrmI AGTACT 3 cut(s) 4901, 5917, 6845
Zsp2I ATGCAT 5 cut(s) 954, 2686, 4611, 6557, 6802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.