pycom12419g00080

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012419
OG0000798
Physical Location & Seq
Forward (+)
100995 .. 106722
5728 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12419g00080.5

Sequence Viewer

Length: 4038 bp
ATGCTAAATAGGCTCCTGCGGCTCGTTCTTGACCATAATATTGCTGATTATGTCACTGCAAAGAACAATGTGGTGTTGTCCTACAAAGATATGAGTCATACAAATTCGTATGGTCTCATACGTGGTCTTCAGTTTGCATCTTTTGTAGTACAATATTATGGGCTTGTGTTGGATCTGTTACTTCTTGGGCTGACTCGAGCCAGTGAGATTGCCGGTCCACCCCAGATGCCAAATGAGTTCATCACTTATTGGGATACAAAGGTTGAGACTCGGCATCCAATCAGGCTGTATTCTAGATACATAGACAGGGTGCATATCTTGTTCCGCTTCACCCACGAGGAGGCTAGGGACCTTATTCAGCGATATCTTACTGAGCATCCGGACCCCAATAATGAAAATATGGTTGGATACAATAATAAGAAATGCTGGCCAAGAGATGCTAGAATGAGGCTTATGAAACATGATGTCAATCTTGGGAGGAGTGTTTTTTGGGATATGAAGAACCGTCTCCCTCGAAGCATCACAACTTTGGAGTGGGAGAACAGCTTTGTGTCTGTGTACAGCAAGGATAATCCAAATTTGCTTTTCAGTATGTCTGGATTTGAAGTTCGGATACTTCCGAAGATAAGAATGAGTCAAGAAGCATTTAGTAACACGAGGGATGGGGTTTGGAATTTGCAGAATGAGCAGACAAAGGAGAGGACTGCAGTTGCTTTCTTACGTGTTGATGATGAACACATGAAGGTGTTTGAGAATCGTGTGAGGCAGATTCTTATGTCTTCAGGGTCTACAACGTTTACAAAAATCGTTAACAAATGGAATACAGCGCTTATTGGTCTAATGACTTACTTCCGTGAAGCAACTGTTCATACACAAGAACTGTTGGATTTGCTCGTCAAATGTGAAAACAAAATTCAGACCCGCATTAAGATTGGGTTGAATTCAAAGATGCCTAGCAGGTTTCCACCTGTCATATTTTACACGCCAAAAGAAATTGGAGGACTTGGCATGTTATCTATGGGTCACATATTGATTCCACAAAGTGACCTCCGTTACAGTCAGCAGACAGATGTTGGTGTGACTCATTTTAGGAGTGGAATGAGTCATGAAGAGGACCAGCTGATCCCAAATCTTTACCGTTACATACAGCCATGGGAGAGTGAATTTATAGATTCTCAACGTGTTTGGGCAGAATATGCACTGAAAAGGCAGGAAGCTCAGGCACAAAATAGGCGTCTGACTCTTGAAGATTTGGAAGATTCATGGGATAGGGGAATACCACGCATAAATACTTTGTTCCAGAAGGACCGGCATACTTTAGCATATGACAAAGGATGGAGAGTTCGGACAGATTTTAAGCAGTACCAGGTGTTGAAACAAAATCCTTTCTGGTGGACACACCAGAGGCATGATGGAAAGTTATGGAACTTAAACAACTACCGAACTGATGTGATTCAAGCCCTTGGAGGTGTTGAAGGAATCCTTGAGCATACATTATTCAAAGGAACATACTTTCCTACATGGGAGGGGCTCTTCTGGGAGAAAGCATCTGGTTTTGAGGAGTCGATGAAGTATAAAAAGTTGACCAATGCACAGAGATCTGGGCTCAATCAAATCCCGAATCGTAGATTTACTCTTTGGTGGTCACCTACCATAAATCGGGCCAACGTATATGTTGGTTTCCAAGTGCAGCTAGATTTAACTGGAATATTTATGCATGGGAAGATTCCAACCTTGAAGATATCACTGATTCAGATATTCCGTGCTCACTTGTGGCAGAAGATTCATGAAAGTGTTGTCATGGATCTCTGTCAGGTTTTGGATCAAGAGTTGGATGCTTTGGAAATTGAAACTGTGCAGAAAGAAACGATACATCCCAGGAAGAGTTACAAAATGAACAGCTCTTGTGCTGACATTCTTCTGTTTGCTGCCCATCGATGGCCAATGTCAAAACCTAGTCTTGTAGCTGAGCCCAAGGATGTCTTTGACCAGAAAGCGAGCAATAAGTACTGGATAGATGTACAACTGCGTTGGGGAGATTATGATTCTCACGATATTGAGCGTTACACCCGAGCGAAGTTTATGGACTATACCACTGACAACATGTCCATCTATCCTTCTCCCACAGGGGTGATGATTGGTCTTGATTTGGCCTATAATTTGCATTCTGCATTTGGTAACTGGTTTCCTGGTTCAAAACCCTTGCTTCAACAGGCAATGAACAAGATCATGAAATCAAATCCAGCCTTATATGTCCTGAGGGAGCGAATAAGGAAAGGATTGCAGCTATATTCTTCTGAGCCTACAGAGCCATATTTATCATCCCAAAACTATGGGGAGATCTTCAGTAATCAAATTATTTGGTTTGTTGATGACACCAATGTGTATCGTGTTACAATCCATAAGACATTTGAAGGAAATCTCACTACAAAACCAATCAATGGTGCTATCTTCATATTCAATCCAAGGACGGGGCAGTTATTCCTGAAGGTTATCCACACTAGTGTCTGGGCAGGACAGAAGCGTCTTGGTCAGTTGGCAAAGTGGAAAACTGCAGAAGAAGTTGCTGCACTTGTGCGTTCTTTGCCAGTGGAAGAACAGCCAAAGCAAATTATTGTGACTCGTAAGGGAATGTTGGATCCCTTAGAGGTTCATTTGCTCGATTTCCCCAACATAGTCATAAAAGGAAGTGAGCTGCAGCTTCCTTTTCAAGCTTGTTTGAAGATTGAGAAGTTTGGTGATCTGATATTGAAGGCTACTGAACCACAGATGGTTCTTTTCAATATTTATGATGATTGGTTGAAGAGTATTTCATCATACACTGCATTCTCTCGGCTAATTTTGATTCTTCGTGCTCTTCATGTGAACAATGAGAAGGCAAAGATGTTATTGAAGCCTGATAAAACAGTCGTTACCGAGCCACATCACATCTGGCCTTCTCTTTCTGATGATCAATGGATGAAGGTTGAGGTTGCTCTTAGGGATCTCATATTGTCAGACTACGCAAAAAAGAACAATGTGAATACATCAGCCCTAACACAATCTGAGATCCGTGACATTATACTTGGAGCTGAGATCACCCCACCTTCGCAACAGAGGCAGCAAATTGCGGAAATAGAAAAACAGGCCAAAGAAGCCAGCCAGCTGACAGCAGTCACAACAAGGACTACAAATGTGCATGGTGATGAACTCATTGTCACCACTACTAGTCCCTATGAGCAAAGTGCATTTGGCTCCAAAACTGATTGGCGTGTGAGAGCAATATCAGCTACAAATCTATATCTCCGTGTCAATCATATATATGTGAATTCAGAAGATATTAAGGAAACGGGTTACACCTATATTATGCCAAAGAACATTTTAAAAAAGTTCATCTGCATTGCGGATCTGAGGACGCAAATAGCTGGTTACTTGTATGGCATAAGCCCACCCGATAACCCTCAGGTCAAGGAAATCCGCTGCATTGCAATGCCACCACAATGGGGTACCCACCAGCAAGTTCATCTTCCATCAGCTCTTCCTGATCATGATTTCCTCAACGATTTGGAGCCTCTAGGATGGATGCACACACAGCCGAATGAGCTTCCTCAGCTTTCTCCTCAGGATCTCACTTCTCATGCTAAGATTTTAGAGAACACCAAGCAATGGGATGGGGAGAAATGCATCATTTTAACCTGCAGTTTCACTCCAGGTTCCTGCTCCTTAACTGCGTACAAGCTTACTCCATCTGGTTATGAATGGGGACGTGTCAATAAAGACACAGGAAGCAATCCTCACGGATACCTTCCCACTCATTACGAGAAGGTGCAGATGCTTCTCAGTGACCGCTTCCTTGGTTTCTATATGATTCCGGACACTGGTCCATGGAACTACAACTTCATGGGGGTGAAGCATACGCCGAGCATGAAGTACGGCATCAAGCTTGGAACACCCAGGGAGTACTATCATGAGGACCACCGGCCGACCCATTACCTTGAGTTCAGCAACCTGGAGGAGGGTGATACAGTGGAAGGAGATCGCGATGATACTTTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

1346

Amino Acids

156.08

Weight (kDa)

8.29

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2523
Acc36I ACCTGC 2 cut(s) 948, 3683
Acc65I GGTACC 1 cut(s) 3485
AccB1I GGYRCC 1 cut(s) 3485
AccB7I CCANNNNNTGG 2 cut(s) 2441, 3645
AccI GTMKAC 1 cut(s) 788
AccII CGCG 1 cut(s) 4020
AccIII TCCGGA 2 cut(s) 379, 3850
AciI CCGC 7 cut(s) 19, 325, 922, 3110, 3383, 3457, 3826
AclI AACGTT 1 cut(s) 794
AcoI YGGCCR 3 cut(s) 428, 1940, 3959
AcsI RAATTY 7 cut(s) 103, 577, 673, 912, 940, 1163, 3307
AcuI CTGAAG 4 cut(s) 113, 765, 2329, 2507
AcyI GRCGYC 1 cut(s) 1234
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 9 cut(s) 150, 560, 1364, 2009, 2022, 3487, 3713, 3911, 3941
AfeI AGCGCT 1 cut(s) 828
AfiI CCNNNNNNNGG 9 cut(s) 340, 1659, 1938, 2441, 2471, 3482, 3645, 3857, 3994
AflIII ACRYGT 4 cut(s) 721, 1180, 2103, 3745
AhdI GACNNNNNGTC 1 cut(s) 2104
AhlI ACTAGT 2 cut(s) 2501, 3206
AjiI CACGTC 1 cut(s) 3746
AjnI CCWGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AleI CACNNNNGTG 3 cut(s) 2129, 2381, 2502
AloI GAACNNNNNNTCC 6 cut(s) 591, 623, 1326, 1358, 1498, 1530
Alw21I GWGCWC 2 cut(s) 1768, 2857
Alw26I GTCTC 3 cut(s) 119, 260, 512
Ama87I CYCGRG 2 cut(s) 195, 2070
Aor13HI TCCGGA 2 cut(s) 379, 3850
Aor51HI AGCGCT 1 cut(s) 828
AoxI GGCC 7 cut(s) 428, 1662, 1940, 2151, 2933, 3126, 3959
ApeKI GCWGC 8 cut(s) 1690, 1928, 2284, 2567, 2695, 2698, 3100, 3459
ApoI RAATTY 7 cut(s) 103, 577, 673, 912, 940, 1163, 3307
ArsI GACNNNNNNTTYG 2 cut(s) 1219, 1251
Asp718I GGTACC 1 cut(s) 3485
AspLEI GCGC 1 cut(s) 829
AspS9I GGNCC 8 cut(s) 215, 349, 382, 1114, 1306, 1662, 3860, 3952
AsuHPI GGTGA 9 cut(s) 322, 1638, 2143, 2750, 3070, 3190, 3194, 3898, 4010
AvaI CYCGRG 2 cut(s) 195, 2070
AvaII GGWCC 7 cut(s) 215, 349, 382, 1114, 1306, 3860, 3952
AxyI CCTNAGG 3 cut(s) 2258, 3441, 3600
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BalI TGGCCA 2 cut(s) 430, 1942
BamHI GGATCC 1 cut(s) 2638
BanI GGYRCC 1 cut(s) 3485
BanII GRGCYC 3 cut(s) 1533, 1608, 1974
BarI GAAGNNNNNNTAC 2 cut(s) 597, 629
BauI CACGAG 2 cut(s) 335, 655
BbsI GAAGAC 2 cut(s) 119, 771
Bbv12I GWGCWC 2 cut(s) 1768, 2857
BbvCI CCTCAGC 1 cut(s) 3588
BbvI GCAGC 8 cut(s) 1702, 1915, 2296, 2554, 2682, 2710, 3112, 3446
BceAI ACGGC 1 cut(s) 3928
BciT130I CCWGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
BciVI GTATCC 4 cut(s) 247, 401, 606, 3773
BclI TGATCA 2 cut(s) 2950, 3523
BcoDI GTCTC 3 cut(s) 119, 260, 512
BcuI ACTAGT 2 cut(s) 2501, 3206
BfaI CTAG 9 cut(s) 294, 345, 441, 954, 1694, 1956, 2502, 3207, 3554
BfmI CTRYAG 5 cut(s) 705, 2304, 2553, 2696, 3676
BfoI RGCGCY 1 cut(s) 830
BfuAI ACCTGC 2 cut(s) 948, 3683
BfuI GTATCC 4 cut(s) 247, 401, 606, 3773
BglII AGATCT 2 cut(s) 1598, 2340
BisI GCNGC 9 cut(s) 20, 1691, 1929, 2285, 2568, 2696, 2699, 3101, 3460
BlpI GCTNAGC 1 cut(s) 1968
BlsI GCNGC 9 cut(s) 21, 1692, 1930, 2286, 2569, 2697, 2700, 3102, 3461
BmcAI AGTACT 2 cut(s) 2009, 3941
Bme1390I CCNGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
Bme18I GGWCC 7 cut(s) 215, 349, 382, 1114, 1306, 3860, 3952
BmeRI GACNNNNNGTC 1 cut(s) 2104
BmeT110I CYCGRG 2 cut(s) 195, 2070
BmgBI CACGTC 1 cut(s) 3746
BmgT120I GGNCC 8 cut(s) 215, 349, 382, 1114, 1306, 1662, 3860, 3952
BmiI GGNNCC 8 cut(s) 14, 350, 384, 2640, 3235, 3487, 3549, 3694
BmrFI CCNGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
BoxI GACNNNNGTC 2 cut(s) 3152, 3858
BpiI GAAGAC 2 cut(s) 119, 771
BpmI CTGGAG 2 cut(s) 3672, 4010
Bpu10I CCTNAGC 2 cut(s) 1218, 3588
Bpu1102I GCTNAGC 1 cut(s) 1968
BpuEI CTTGAG 2 cut(s) 1505, 3995
Bsa29I ATCGAT 1 cut(s) 1936
BsaAI YACGTR 2 cut(s) 122, 722
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 1234
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 9 cut(s) 1151, 1462, 1877, 1974, 2465, 3832, 3863, 3932, 3933
BsaWI WCCGGW 2 cut(s) 379, 3850
Bsc4I CCNNNNNNNGG 9 cut(s) 340, 1659, 1938, 2441, 2471, 3482, 3645, 3857, 3994
Bse118I RCCGGY 3 cut(s) 212, 1308, 3957
Bse1I ACTGG 6 cut(s) 201, 1708, 2015, 2186, 2588, 3862
Bse21I CCTNAGG 3 cut(s) 2258, 3441, 3600
Bse3DI GCAATG 5 cut(s) 2223, 3378, 3462, 3474, 3650
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 2 cut(s) 379, 3850
BseBI CCWGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
BseCI ATCGAT 1 cut(s) 1936
BseDI CCNNGG 9 cut(s) 1151, 1462, 1877, 1974, 2465, 3832, 3863, 3932, 3933
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 9 cut(s) 340, 1659, 1938, 2441, 2471, 3482, 3645, 3857, 3994
BseMI GCAATG 5 cut(s) 2223, 3378, 3462, 3474, 3650
BseNI ACTGG 6 cut(s) 201, 1708, 2015, 2186, 2588, 3862
BseRI GAGGAG 5 cut(s) 353, 493, 1574, 3588, 4007
BseX3I CGGCCG 1 cut(s) 3959
BseXI GCAGC 8 cut(s) 1702, 1915, 2296, 2554, 2682, 2710, 3112, 3446
BsgI GTGCAG 4 cut(s) 1709, 1877, 2553, 3827
Bsh1236I CGCG 1 cut(s) 4020
Bsh1285I CGRYCG 1 cut(s) 3962
BshFI GGCC 7 cut(s) 430, 1664, 1942, 2153, 2935, 3128, 3961
BshNI GGYRCC 1 cut(s) 3485
BshVI ATCGAT 1 cut(s) 1936
BsiEI CGRYCG 1 cut(s) 3962
BsiHKAI GWGCWC 2 cut(s) 1768, 2857
BsiHKCI CYCGRG 2 cut(s) 195, 2070
BsiSI CCGG 5 cut(s) 213, 380, 1309, 3851, 3958
BslFI GGGAC 3 cut(s) 362, 3195, 3756
BslI CCNNNNNNNGG 9 cut(s) 340, 1659, 1938, 2441, 2471, 3482, 3645, 3857, 3994
BsmAI GTCTC 3 cut(s) 119, 260, 512
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 3 cut(s) 362, 3195, 3756
BsmI GAATGC 2 cut(s) 2164, 2825
BsnI GGCC 7 cut(s) 430, 1664, 1942, 2153, 2935, 3128, 3961
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 2 cut(s) 195, 2070
Bsp1286I GDGCHC 5 cut(s) 1533, 1608, 1768, 1974, 2857
Bsp13I TCCGGA 2 cut(s) 379, 3850
Bsp1407I TGTACA 2 cut(s) 558, 2020
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 2 cut(s) 1151, 3863
Bsp68I TCGCGA 1 cut(s) 4020
BspACI CCGC 7 cut(s) 19, 325, 922, 3110, 3383, 3457, 3826
BspANI GGCC 7 cut(s) 430, 1664, 1942, 2153, 2935, 3128, 3961
BspDI ATCGAT 1 cut(s) 1936
BspEI TCCGGA 2 cut(s) 379, 3850
BspFNI CGCG 1 cut(s) 4020
BspHI TCATGA 5 cut(s) 1105, 1786, 2229, 3526, 3946
BspLI GGNNCC 8 cut(s) 14, 350, 384, 2640, 3235, 3487, 3549, 3694
BspMAI CTGCAG 4 cut(s) 709, 2557, 2700, 3680
BspMI ACCTGC 2 cut(s) 948, 3683
BspQI GCTCTTC 3 cut(s) 1538, 2862, 3522
BspT107I GGYRCC 1 cut(s) 3485
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 5 cut(s) 2223, 3378, 3462, 3474, 3650
BsrFI RCCGGY 3 cut(s) 212, 1308, 3957
BsrGI TGTACA 2 cut(s) 558, 2020
BsrI ACTGG 6 cut(s) 201, 1708, 2015, 2186, 2588, 3862
BssAI RCCGGY 3 cut(s) 212, 1308, 3957
BssECI CCNNGG 9 cut(s) 1151, 1462, 1877, 1974, 2465, 3832, 3863, 3932, 3933
BssNI GRCGYC 1 cut(s) 1234
BssSI CACGAG 2 cut(s) 335, 655
BssT1I CCWWGG 6 cut(s) 1151, 1462, 1974, 2465, 3832, 3863
Bst2BI CACGAG 2 cut(s) 335, 655
Bst2UI CCWGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
Bst4CI ACNGT 8 cut(s) 506, 865, 882, 1058, 1139, 1855, 2908, 4006
Bst6I CTCTTC 6 cut(s) 1104, 1538, 1877, 2798, 2862, 3522
BstACI GRCGYC 1 cut(s) 1234
BstAPI GCANNNNNTGC 1 cut(s) 1196
BstAUI TGTACA 2 cut(s) 558, 2020
BstBAI YACGTR 2 cut(s) 122, 722
BstC8I GCNNGC 4 cut(s) 428, 1999, 3139, 3143
BstDSI CCRYGG 2 cut(s) 1151, 3863
BstEII GGTNACC 1 cut(s) 1644
BstENI CCTNNNNNAGG 1 cut(s) 3992
BstFNI CGCG 1 cut(s) 4020
BstH2I RGCGCY 1 cut(s) 830
BstHHI GCGC 1 cut(s) 829
BstMAI GTCTC 3 cut(s) 119, 260, 512
BstMCI CGRYCG 1 cut(s) 3962
BstNI CCWGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
BstNSI RCATGY 2 cut(s) 1012, 2107
BstPAI GACNNNNGTC 2 cut(s) 3152, 3858
BstPI GGTNACC 1 cut(s) 1644
BstSCI CCNGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
BstSFI CTRYAG 5 cut(s) 705, 2304, 2553, 2696, 3676
BstUI CGCG 1 cut(s) 4020
BstV1I GCAGC 8 cut(s) 1702, 1915, 2296, 2554, 2682, 2710, 3112, 3446
BstV2I GAAGAC 2 cut(s) 119, 771
BstX2I RGATCY 9 cut(s) 172, 1598, 1804, 2340, 2638, 2983, 3048, 3385, 3604
BstXI CCANNNNNNTGG 2 cut(s) 2333, 3480
BstYI RGATCY 9 cut(s) 172, 1598, 1804, 2340, 2638, 2983, 3048, 3385, 3604
BstZI CGGCCG 1 cut(s) 3959
Bsu15I ATCGAT 1 cut(s) 1936
Bsu36I CCTNAGG 3 cut(s) 2258, 3441, 3600
BsuI GTATCC 4 cut(s) 247, 401, 606, 3773
BsuRI GGCC 7 cut(s) 430, 1664, 1942, 2153, 2935, 3128, 3961
BsuTUI ATCGAT 1 cut(s) 1936
BtgI CCRYGG 2 cut(s) 1151, 3863
BtrI CACGTC 1 cut(s) 3746
BtsI GCAGTG 2 cut(s) 54, 2820
BtuMI TCGCGA 1 cut(s) 4020
BveI ACCTGC 2 cut(s) 948, 3683
Cac8I GCNNGC 4 cut(s) 428, 1999, 3139, 3143
CciI TCATGA 5 cut(s) 1105, 1786, 2229, 3526, 3946
CfoI GCGC 1 cut(s) 829
Cfr10I RCCGGY 3 cut(s) 212, 1308, 3957
Cfr13I GGNCC 8 cut(s) 215, 349, 382, 1114, 1306, 1662, 3860, 3952
ClaI ATCGAT 1 cut(s) 1936
CseI GACGC 3 cut(s) 1223, 2513, 3403
CsiI ACCWGGT 1 cut(s) 1365
Csp6I GTAC 9 cut(s) 149, 559, 1363, 2008, 2021, 3486, 3712, 3910, 3940
CviQI GTAC 9 cut(s) 149, 559, 1363, 2008, 2021, 3486, 3712, 3910, 3940
DraI TTTAAA 1 cut(s) 3363
DraIII CACNNNGTG 1 cut(s) 1043
DrdI GACNNNNNNGTC 1 cut(s) 2523
DriI GACNNNNNGTC 1 cut(s) 2104
DseDI GACNNNNNNGTC 1 cut(s) 2523
EaeI YGGCCR 3 cut(s) 428, 1940, 3959
EagI CGGCCG 1 cut(s) 3959
Eam1104I CTCTTC 6 cut(s) 1104, 1538, 1877, 2798, 2862, 3522
Eam1105I GACNNNNNGTC 1 cut(s) 2104
EarI CTCTTC 6 cut(s) 1104, 1538, 1877, 2798, 2862, 3522
EclXI CGGCCG 1 cut(s) 3959
Eco130I CCWWGG 6 cut(s) 1151, 1462, 1974, 2465, 3832, 3863
Eco24I GRGCYC 3 cut(s) 1533, 1608, 1974
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 2 cut(s) 365, 1743
Eco47I GGWCC 7 cut(s) 215, 349, 382, 1114, 1306, 3860, 3952
Eco47III AGCGCT 1 cut(s) 828
Eco52I CGGCCG 1 cut(s) 3959
Eco57I CTGAAG 4 cut(s) 113, 765, 2329, 2507
Eco81I CCTNAGG 3 cut(s) 2258, 3441, 3600
Eco88I CYCGRG 2 cut(s) 195, 2070
Eco91I GGTNACC 1 cut(s) 1644
EcoNI CCTNNNNNAGG 1 cut(s) 3992
EcoO109I RGGNCCY 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 1644
EcoRI GAATTC 2 cut(s) 940, 3307
EcoRII CCWGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
EcoRV GATATC 2 cut(s) 365, 1743
EcoT14I CCWWGG 6 cut(s) 1151, 1462, 1974, 2465, 3832, 3863
EcoT22I ATGCAT 2 cut(s) 1719, 3665
EcoT38I GRGCYC 3 cut(s) 1533, 1608, 1974
ErhI CCWWGG 6 cut(s) 1151, 1462, 1974, 2465, 3832, 3863
Esp3I CGTCTC 1 cut(s) 512
FalI AAGNNNNNCTT 6 cut(s) 1467, 1499, 1967, 1999, 3489, 3521
FaqI GGGAC 3 cut(s) 362, 3195, 3756
FauI CCCGC 1 cut(s) 929
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 2 cut(s) 2950, 3523
FblI GTMKAC 1 cut(s) 788
Fnu4HI GCNGC 9 cut(s) 20, 1691, 1929, 2285, 2568, 2696, 2699, 3101, 3460
FriOI GRGCYC 3 cut(s) 1533, 1608, 1974
Fsp4HI GCNGC 9 cut(s) 20, 1691, 1929, 2285, 2568, 2696, 2699, 3101, 3460
FspBI CTAG 9 cut(s) 294, 345, 441, 954, 1694, 1956, 2502, 3207, 3554
GlaI GCGC 1 cut(s) 828
GluI GCNGC 9 cut(s) 20, 1691, 1929, 2285, 2568, 2696, 2699, 3101, 3460
GsuI CTGGAG 2 cut(s) 3672, 4010
HaeII RGCGCY 1 cut(s) 830
HaeIII GGCC 7 cut(s) 430, 1664, 1942, 2153, 2935, 3128, 3961
HapII CCGG 5 cut(s) 213, 380, 1309, 3851, 3958
HgaI GACGC 3 cut(s) 1223, 2513, 3403
HhaI GCGC 1 cut(s) 829
Hin1I GRCGYC 1 cut(s) 1234
Hin6I GCGC 1 cut(s) 827
HinP1I GCGC 1 cut(s) 827
HincII GTYRAC 2 cut(s) 811, 1584
HindII GTYRAC 2 cut(s) 811, 1584
HindIII AAGCTT 3 cut(s) 2712, 3716, 3920
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 5 cut(s) 213, 380, 1309, 3851, 3958
HphI GGTGA 9 cut(s) 322, 1638, 2143, 2750, 3070, 3190, 3194, 3898, 4010
Hpy166II GTNNAC 8 cut(s) 218, 559, 789, 798, 811, 1395, 1584, 2866
Hpy8I GTNNAC 8 cut(s) 218, 559, 789, 798, 811, 1395, 1584, 2866
HpyCH4III ACNGT 8 cut(s) 506, 865, 882, 1058, 1139, 1855, 2908, 4006
HpyCH4IV ACGT 6 cut(s) 121, 721, 794, 1180, 1668, 3745
HpySE526I ACGT 6 cut(s) 121, 721, 794, 1180, 1668, 3745
Hsp92I GRCGYC 1 cut(s) 1234
HspAI GCGC 1 cut(s) 827
Kpn2I TCCGGA 2 cut(s) 379, 3850
KpnI GGTACC 1 cut(s) 3489
Ksp22I TGATCA 2 cut(s) 2950, 3523
KspAI GTTAAC 1 cut(s) 811
LguI GCTCTTC 3 cut(s) 1538, 2862, 3522
LmnI GCTCC 6 cut(s) 18, 2263, 3068, 3239, 3547, 3704
Lsp1109I GCAGC 8 cut(s) 1702, 1915, 2296, 2554, 2682, 2710, 3112, 3446
MabI ACCWGGT 1 cut(s) 1365
MaeI CTAG 9 cut(s) 294, 345, 441, 954, 1694, 1956, 2502, 3207, 3554
MaeII ACGT 6 cut(s) 121, 721, 794, 1180, 1668, 3745
MflI RGATCY 9 cut(s) 172, 1598, 1804, 2340, 2638, 2983, 3048, 3385, 3604
MhlI GDGCHC 5 cut(s) 1533, 1608, 1768, 1974, 2857
MlsI TGGCCA 2 cut(s) 430, 1942
MluNI TGGCCA 2 cut(s) 430, 1942
MlyI GAGTC 9 cut(s) 103, 187, 262, 643, 1075, 1111, 1234, 1571, 2614
MmeI TCCRAC 6 cut(s) 150, 385, 864, 1754, 1812, 2616
Mox20I TGGCCA 2 cut(s) 430, 1942
Mph1103I ATGCAT 2 cut(s) 1719, 3665
MroI TCCGGA 2 cut(s) 379, 3850
MscI TGGCCA 2 cut(s) 430, 1942
MseI TTAA 9 cut(s) 810, 927, 1356, 1430, 1700, 3321, 3362, 3671, 3704
Msp20I TGGCCA 2 cut(s) 430, 1942
MspA1I CMGCKG 3 cut(s) 1120, 3145, 3459
MspI CCGG 5 cut(s) 213, 380, 1309, 3851, 3958
MspR9I CCNGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
Mva1269I GAATGC 2 cut(s) 2164, 2825
MvaI CCWGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
MvnI CGCG 1 cut(s) 4020
NcoI CCATGG 2 cut(s) 1151, 3863
NdeI CATATG 1 cut(s) 1324
NlaIV GGNNCC 8 cut(s) 14, 350, 384, 2640, 3235, 3487, 3549, 3694
NmeAIII GCCGAG 3 cut(s) 250, 2812, 3924
NruI TCGCGA 1 cut(s) 4020
NsiI ATGCAT 2 cut(s) 1719, 3665
NspI RCATGY 2 cut(s) 1012, 2107
OliI CACNNNNGTG 3 cut(s) 2129, 2381, 2502
PaeR7I CTCGAG 1 cut(s) 195
PagI TCATGA 5 cut(s) 1105, 1786, 2229, 3526, 3946
PasI CCCWGGG 1 cut(s) 3933
PciI ACATGT 1 cut(s) 2103
PciSI GCTCTTC 3 cut(s) 1538, 2862, 3522
PctI GAATGC 2 cut(s) 2164, 2825
PflMI CCANNNNNTGG 2 cut(s) 2441, 3645
PkrI GCNGC 9 cut(s) 21, 1692, 1930, 2286, 2569, 2697, 2700, 3102, 3461
PleI GAGTC 9 cut(s) 102, 187, 262, 642, 1075, 1110, 1234, 1570, 2614
PpsI GAGTC 9 cut(s) 102, 187, 262, 642, 1075, 1110, 1234, 1570, 2614
Ppu21I YACGTR 2 cut(s) 122, 722
PpuMI RGGWCCY 1 cut(s) 349
PscI ACATGT 1 cut(s) 2103
PshAI GACNNNNGTC 2 cut(s) 3152, 3858
Psp1406I AACGTT 1 cut(s) 794
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
PspEI GGTNACC 1 cut(s) 1644
PspGI CCWGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
PspN4I GGNNCC 8 cut(s) 14, 350, 384, 2640, 3235, 3487, 3549, 3694
PspPI GGNCC 8 cut(s) 215, 349, 382, 1114, 1306, 1662, 3860, 3952
PspPPI RGGWCCY 1 cut(s) 349
PspXI VCTCGAGB 1 cut(s) 195
PstI CTGCAG 4 cut(s) 709, 2557, 2700, 3680
PsuI RGATCY 9 cut(s) 172, 1598, 1804, 2340, 2638, 2983, 3048, 3385, 3604
PvuII CAGCTG 2 cut(s) 1120, 3145
RruI TCGCGA 1 cut(s) 4020
RsaI GTAC 9 cut(s) 150, 560, 1364, 2009, 2022, 3487, 3713, 3911, 3941
RsaNI GTAC 9 cut(s) 149, 559, 1363, 2008, 2021, 3486, 3712, 3910, 3940
SapI GCTCTTC 3 cut(s) 1538, 2862, 3522
SaqAI TTAA 9 cut(s) 810, 927, 1356, 1430, 1700, 3321, 3362, 3671, 3704
SatI GCNGC 9 cut(s) 20, 1691, 1929, 2285, 2568, 2696, 2699, 3101, 3460
Sau96I GGNCC 8 cut(s) 215, 349, 382, 1114, 1306, 1662, 3860, 3952
ScaI AGTACT 2 cut(s) 2009, 3941
SchI GAGTC 9 cut(s) 103, 187, 262, 643, 1075, 1111, 1234, 1571, 2614
ScrFI CCNGG 6 cut(s) 1367, 1879, 2190, 3690, 3934, 3989
SduI GDGCHC 5 cut(s) 1533, 1608, 1768, 1974, 2857
SexAI ACCWGGT 1 cut(s) 1365
SfcI CTRYAG 5 cut(s) 705, 2304, 2553, 2696, 3676
Sfr274I CTCGAG 1 cut(s) 195
SinI GGWCC 7 cut(s) 215, 349, 382, 1114, 1306, 3860, 3952
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 3 cut(s) 195, 1484, 3974
SmoI CTYRAG 3 cut(s) 195, 1484, 3974
SpeI ACTAGT 2 cut(s) 2501, 3206
SsiI CCGC 7 cut(s) 19, 325, 922, 3110, 3383, 3457, 3826
SspI AATATT 4 cut(s) 40, 155, 1710, 2785
SspMI CTAG 9 cut(s) 294, 345, 441, 954, 1694, 1956, 2502, 3207, 3554
StyD4I CCNGG 6 cut(s) 1365, 1877, 2188, 3688, 3932, 3987
StyI CCWWGG 6 cut(s) 1151, 1462, 1974, 2465, 3832, 3863
TaaI ACNGT 8 cut(s) 506, 865, 882, 1058, 1139, 1855, 2908, 4006
TaiI ACGT 6 cut(s) 124, 724, 797, 1183, 1671, 3748
TaqI TCGA 5 cut(s) 196, 514, 1565, 1936, 2661
TatI WGTACW 5 cut(s) 148, 558, 2007, 2020, 3939
TauI GCSGC 1 cut(s) 22
Tru1I TTAA 9 cut(s) 810, 927, 1356, 1430, 1700, 3321, 3362, 3671, 3704
Tru9I TTAA 9 cut(s) 810, 927, 1356, 1430, 1700, 3321, 3362, 3671, 3704
TseI GCWGC 8 cut(s) 1690, 1928, 2284, 2567, 2695, 2698, 3100, 3459
TspGWI ACGGA 6 cut(s) 842, 1040, 1751, 3041, 3275, 3792
Van91I CCANNNNNTGG 2 cut(s) 2441, 3645
VpaK11BI GGWCC 7 cut(s) 215, 349, 382, 1114, 1306, 3860, 3952
XagI CCTNNNNNAGG 1 cut(s) 3992
XapI RAATTY 7 cut(s) 103, 577, 673, 912, 940, 1163, 3307
XbaI TCTAGA 1 cut(s) 293
XceI RCATGY 2 cut(s) 1012, 2107
XcmI CCANNNNNNNNNTGG 2 cut(s) 1409, 2928
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 788
XspI CTAG 9 cut(s) 294, 345, 441, 954, 1694, 1956, 2502, 3207, 3554
ZrmI AGTACT 2 cut(s) 2009, 3941
Zsp2I ATGCAT 2 cut(s) 1719, 3665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.