AT3G07460

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000944
Physical Location & Seq
Reverse (-)
2384375 .. 2385982
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07460.1

Sequence Viewer

Length: 534 bp
ATGCTTCGTATAGTTCAAATCACGCTTCTTTGTTTCGTTCTCGCCGCCGGGATCTCGATCTCCGCCGTGATCGCCGAGAATGAATCTATAGACGAGATCCTTCTAGCGAATGGATTACCTTTGGGGCTATTTCCTAAAGGCGTGAAAGGATTTACCGTTAATGGCGAAACGGGACGGTTCTCGGTTTATTTGAATCAGTCATGCCAGGCTAAGTATGAGACAGAGCTGCATTACGATGAGATTGTTTCGGGAACGATAGGTTATGCTCAAATCAGGGATTTATCGGGAATCTCAGCGCAAGAGCTGTTCTTGTGGTTACAGGTGAAAGGAATCCGAGTCGACGTGCCTAGCTCTGGTCTTATATTCTTCGACGTTGGTGTTCTTCGCAAGCAATACTCTTTGTCTTTGTTCGAGACACCAAGAGATTGTGTCGCGGTTCGTGGCGATGCCGAGTTTATTGGTGAAAACAAGGTTCAGTCTTCGATGTTGCCATTGTATCAAGTAGATCAAACTCTTGGAAGAAACACTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

177

Amino Acids

19.44

Weight (kDa)

4.88

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 138 3.4e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 339
AccII CGCG 1 cut(s) 434
AciI CCGC 3 cut(s) 45, 63, 434
AclWI GGATC 2 cut(s) 59, 91
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 17, 193
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 3 cut(s) 226, 304, 351
AluI AGCT 3 cut(s) 226, 304, 351
Alw26I GTCTC 2 cut(s) 212, 407
AlwI GGATC 2 cut(s) 59, 91
ApeKI GCWGC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 334, 473
BbsI GAAGAC 1 cut(s) 471
BbvI GCAGC 1 cut(s) 213
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 206
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 212, 407
BfaI CTAG 2 cut(s) 104, 348
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 2 cut(s) 45, 227
BlsI GCNGC 2 cut(s) 46, 228
Bme1390I CCNGG 2 cut(s) 49, 206
BmgBI CACGTC 1 cut(s) 343
BmrFI CCNGG 2 cut(s) 49, 206
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 56
BseBI CCWGG 1 cut(s) 206
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 1 cut(s) 306
BseXI GCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 434
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 2 cut(s) 212, 407
BsmFI GGGAC 1 cut(s) 186
Bsp143I GATC 5 cut(s) 51, 57, 69, 96, 505
BspACI CCGC 3 cut(s) 45, 63, 434
BspCNI CTCAG 1 cut(s) 305
BspFNI CGCG 1 cut(s) 434
BspPI GGATC 2 cut(s) 59, 91
BssMI GATC 5 cut(s) 51, 57, 69, 96, 505
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 3 cut(s) 157, 177, 529
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 210, 292
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 5 cut(s) 54, 60, 72, 99, 508
BstMAI GTCTC 2 cut(s) 212, 407
BstMBI GATC 5 cut(s) 51, 57, 69, 96, 505
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 47, 204
BstSFI CTRYAG 1 cut(s) 87
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 2 cut(s) 51, 96
BstYI RGATCY 2 cut(s) 51, 96
BtgZI GCGATG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 525
Cac8I GCNNGC 1 cut(s) 389
CfoI GCGC 1 cut(s) 298
CviAII CATG 1 cut(s) 201
CviJI RGCY 5 cut(s) 127, 209, 226, 304, 351
CviKI_1 RGCY 5 cut(s) 127, 209, 226, 304, 351
DdeI CTNAG 2 cut(s) 210, 292
DpnI GATC 5 cut(s) 53, 59, 71, 98, 507
DpnII GATC 5 cut(s) 51, 57, 69, 96, 505
EciI GGCGGA 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 204
FaeI CATG 1 cut(s) 204
FaiI YATR 6 cut(s) 11, 89, 202, 216, 264, 362
FaqI GGGAC 1 cut(s) 186
FatI CATG 1 cut(s) 200
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 2 cut(s) 45, 227
Fsp4HI GCNGC 2 cut(s) 45, 227
FspBI CTAG 2 cut(s) 104, 348
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 45, 227
HapII CCGG 1 cut(s) 48
HhaI GCGC 1 cut(s) 298
Hin1II CATG 1 cut(s) 204
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 5 cut(s) 83, 193, 288, 330, 336
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 334, 473
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 335
Hpy188III TCNNGA 4 cut(s) 55, 249, 285, 412
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 2 cut(s) 344, 374
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 3 cut(s) 157, 177, 529
HpyCH4IV ACGT 2 cut(s) 342, 372
HpyCH4V TGCA 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 210, 292
HpySE526I ACGT 2 cut(s) 342, 372
Hsp92II CATG 1 cut(s) 204
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 5 cut(s) 51, 57, 69, 96, 505
LpnPI CCDG 6 cut(s) 61, 191, 218, 259, 305, 339
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 1 cut(s) 436
MaeI CTAG 2 cut(s) 104, 348
MaeII ACGT 2 cut(s) 342, 372
MaeIII GTNAC 1 cut(s) 315
MalI GATC 5 cut(s) 53, 59, 71, 98, 507
MboI GATC 5 cut(s) 51, 57, 69, 96, 505
MboII GAAGA 4 cut(s) 358, 374, 471, 531
MflI RGATCY 2 cut(s) 51, 96
MlyI GAGTC 1 cut(s) 345
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 49, 206
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 49
NdeII GATC 5 cut(s) 51, 57, 69, 96, 505
NlaIII CATG 1 cut(s) 204
NmeAIII GCCGAG 2 cut(s) 100, 475
PfeI GAWTC 4 cut(s) 83, 193, 288, 330
PkrI GCNGC 2 cut(s) 46, 228
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PsuI RGATCY 2 cut(s) 51, 96
RseI CAYNNNNRTG 1 cut(s) 234
SalI GTCGAC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 2 cut(s) 45, 227
Sau3AI GATC 5 cut(s) 51, 57, 69, 96, 505
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 2 cut(s) 49, 206
SetI ASST 9 cut(s) 121, 228, 262, 306, 324, 345, 353, 375, 474
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 234
SsiI CCGC 3 cut(s) 45, 63, 434
SspMI CTAG 2 cut(s) 104, 348
StyD4I CCNGG 2 cut(s) 47, 204
TaaI ACNGT 3 cut(s) 157, 177, 529
TaiI ACGT 2 cut(s) 345, 375
TaqI TCGA 5 cut(s) 56, 339, 369, 411, 482
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 4 cut(s) 83, 193, 288, 330
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 532
TseI GCWGC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 2 cut(s) 104, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.