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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 251 |
| AccII | CGCG | 1 cut(s) | 147 |
| AciI | CCGC | 3 cut(s) | 116, 132, 284 |
| AclI | AACGTT | 1 cut(s) | 345 |
| AclWI | GGATC | 1 cut(s) | 306 |
| AluBI | AGCT | 1 cut(s) | 151 |
| AluI | AGCT | 1 cut(s) | 151 |
| AlwI | GGATC | 1 cut(s) | 306 |
| ApeKI | GCWGC | 2 cut(s) | 5, 113 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 291 |
| AsuHPI | GGTGA | 1 cut(s) | 229 |
| BbvI | GCAGC | 1 cut(s) | 100 |
| BceAI | ACGGC | 1 cut(s) | 331 |
| BfmI | CTRYAG | 1 cut(s) | 356 |
| BisI | GCNGC | 2 cut(s) | 6, 114 |
| BlsI | GCNGC | 2 cut(s) | 7, 115 |
| BpuEI | CTTGAG | 1 cut(s) | 206 |
| Bse1I | ACTGG | 1 cut(s) | 81 |
| BseNI | ACTGG | 1 cut(s) | 81 |
| BseXI | GCAGC | 1 cut(s) | 100 |
| Bsh1236I | CGCG | 1 cut(s) | 147 |
| Bsp143I | GATC | 1 cut(s) | 298 |
| Bsp68I | TCGCGA | 1 cut(s) | 147 |
| BspACI | CCGC | 3 cut(s) | 116, 132, 284 |
| BspFNI | CGCG | 1 cut(s) | 147 |
| BspPI | GGATC | 1 cut(s) | 306 |
| BsrI | ACTGG | 1 cut(s) | 81 |
| BssMI | GATC | 1 cut(s) | 298 |
| BstC8I | GCNNGC | 2 cut(s) | 35, 149 |
| BstDEI | CTNAG | 1 cut(s) | 152 |
| BstFNI | CGCG | 1 cut(s) | 147 |
| BstKTI | GATC | 1 cut(s) | 301 |
| BstMBI | GATC | 1 cut(s) | 298 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 122, 131 |
| BstNSI | RCATGY | 1 cut(s) | 37 |
| BstSFI | CTRYAG | 1 cut(s) | 356 |
| BstUI | CGCG | 1 cut(s) | 147 |
| BstV1I | GCAGC | 1 cut(s) | 100 |
| BtuMI | TCGCGA | 1 cut(s) | 147 |
| Cac8I | GCNNGC | 2 cut(s) | 35, 149 |
| CviAII | CATG | 2 cut(s) | 21, 34 |
| CviJI | RGCY | 3 cut(s) | 5, 151, 337 |
| CviKI_1 | RGCY | 3 cut(s) | 5, 151, 337 |
| DdeI | CTNAG | 1 cut(s) | 152 |
| DpnI | GATC | 1 cut(s) | 300 |
| DpnII | GATC | 1 cut(s) | 298 |
| FaeI | CATG | 2 cut(s) | 24, 37 |
| FaiI | YATR | 8 cut(s) | 22, 35, 47, 197, 237, 239, 251, 305 |
| FatI | CATG | 2 cut(s) | 20, 33 |
| Fnu4HI | GCNGC | 2 cut(s) | 6, 114 |
| Fsp4HI | GCNGC | 2 cut(s) | 6, 114 |
| GluI | GCNGC | 2 cut(s) | 6, 114 |
| Hin1II | CATG | 2 cut(s) | 24, 37 |
| HinfI | GANTC | 1 cut(s) | 244 |
| HphI | GGTGA | 1 cut(s) | 229 |
| Hpy166II | GTNNAC | 1 cut(s) | 166 |
| Hpy188I | TCNGA | 3 cut(s) | 16, 43, 64 |
| Hpy188III | TCNNGA | 3 cut(s) | 106, 146, 296 |
| Hpy8I | GTNNAC | 1 cut(s) | 166 |
| HpyAV | CCTTC | 2 cut(s) | 314, 321 |
| HpyCH4IV | ACGT | 3 cut(s) | 193, 289, 345 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 122, 131 |
| HpyF3I | CTNAG | 1 cut(s) | 152 |
| HpySE526I | ACGT | 3 cut(s) | 193, 289, 345 |
| Hsp92II | CATG | 2 cut(s) | 24, 37 |
| Kzo9I | GATC | 1 cut(s) | 298 |
| LpnPI | CCDG | 4 cut(s) | 62, 77, 193, 281 |
| Lsp1109I | GCAGC | 1 cut(s) | 100 |
| MaeII | ACGT | 3 cut(s) | 193, 289, 345 |
| MalI | GATC | 1 cut(s) | 300 |
| MboI | GATC | 1 cut(s) | 298 |
| MboII | GAAGA | 1 cut(s) | 343 |
| MluCI | AATT | 1 cut(s) | 87 |
| MnlI | CCTC | 5 cut(s) | 181, 213, 256, 267, 304 |
| MroXI | GAANNNNTTC | 1 cut(s) | 291 |
| MvnI | CGCG | 1 cut(s) | 147 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 122, 131 |
| NdeII | GATC | 1 cut(s) | 298 |
| NlaIII | CATG | 2 cut(s) | 24, 37 |
| NruI | TCGCGA | 1 cut(s) | 147 |
| NspI | RCATGY | 1 cut(s) | 37 |
| PaeI | GCATGC | 1 cut(s) | 37 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 315 |
| PdmI | GAANNNNTTC | 1 cut(s) | 291 |
| PfeI | GAWTC | 1 cut(s) | 244 |
| PkrI | GCNGC | 2 cut(s) | 7, 115 |
| PsiI | TTATAA | 1 cut(s) | 251 |
| Psp1406I | AACGTT | 1 cut(s) | 345 |
| RruI | TCGCGA | 1 cut(s) | 147 |
| SatI | GCNGC | 2 cut(s) | 6, 114 |
| Sau3AI | GATC | 1 cut(s) | 298 |
| SetI | ASST | 6 cut(s) | 96, 153, 196, 219, 292, 348 |
| SfcI | CTRYAG | 1 cut(s) | 356 |
| SmlI | CTYRAG | 1 cut(s) | 185 |
| SmoI | CTYRAG | 1 cut(s) | 185 |
| SphI | GCATGC | 1 cut(s) | 37 |
| Sse9I | AATT | 1 cut(s) | 87 |
| SsiI | CCGC | 3 cut(s) | 116, 132, 284 |
| TaiI | ACGT | 3 cut(s) | 196, 292, 348 |
| TaqI | TCGA | 1 cut(s) | 172 |
| TasI | AATT | 1 cut(s) | 87 |
| TfiI | GAWTC | 1 cut(s) | 244 |
| TseI | GCWGC | 2 cut(s) | 5, 113 |
| TspDTI | ATGAA | 2 cut(s) | 195, 212 |
| TspGWI | ACGGA | 1 cut(s) | 256 |
| XceI | RCATGY | 1 cut(s) | 37 |
| XmnI | GAANNNNTTC | 1 cut(s) | 291 |