Rw1G005660

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000944
Physical Location & Seq
Forward (+)
11359164 .. 11359781
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005660.1

Sequence Viewer

Length: 585 bp
ATGGCATTGGGATTGCAGGGTTCAAAATCCCTCGGGGGCTTTGTTCTTCTGCTCCTCATTGATGCGATCGGCGCAGTGGCCGGAGCATCGCCAGCCCCAAAATCCAGTAACAAAACAAGCATCGGAAAAGGAGGAGGAGGAAGCATCTACGATCACCTCCGGCAATATGGGTTACCCATCGACCTCCTCCCCAAAGGCATCACGGAATATTCCCTCGACGATTCCAGCGGCGAATTCCTGGTGTTTCTGCCCCAGCCTTGCCGTACCAAGTTTGATAATCAAGTCTGGTACGATTTCAACGTCTCTGGGACTCTCACTTTAGGTCGAATTGCCGACTTATCTGGTGTCTCCTCTCAGGAACTCTTCCTCTGGTTTCTGGTGAAAGGTATCCGAGTCGATGTGCCCAGCTCCGGCCTCATTTACTTCAATGTCGGAGTTACTCCACCCGATTTCACTGCTCTTGATCCTTTTGATCCCTCATCTCCTTCTTCTTCTTCCAATTCCCCATCTGAGCTCAAGCTCAAGGTAGATTGTGATTCTGATTCCAATTCCAATTCTCAATTCGATTCCATTGCTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

194

Amino Acids

20.54

Weight (kDa)

4.51

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 49 - 148 3.9e-27 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 228
AclWI GGATC 2 cut(s) 458, 467
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 233
AfaI GTAC 2 cut(s) 265, 290
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 24, 298, 427
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 545, 577
AluBI AGCT 3 cut(s) 408, 514, 520
AluI AGCT 3 cut(s) 408, 514, 520
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 2 cut(s) 307, 352
AlwI GGATC 2 cut(s) 458, 467
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 2 cut(s) 78, 412
ApoI RAATTY 1 cut(s) 233
AspLEI GCGC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 146, 391
AvaI CYCGRG 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 516
BccI CCATC 2 cut(s) 185, 514
BceAI ACGGC 1 cut(s) 246
BcgI CGANNNNNNTGC 3 cut(s) 437, 471, 554
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 398
BcoDI GTCTC 2 cut(s) 307, 352
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 5 cut(s) 52, 95, 129, 153, 207
BpuEI CTTGAG 2 cut(s) 500, 506
BsaJI CCNNGG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 2 cut(s) 368, 501
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 5 cut(s) 44, 147, 150, 176, 340
BseSI GKGCMC 1 cut(s) 405
BseYI CCCAGC 2 cut(s) 252, 404
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 2 cut(s) 80, 414
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 3 cut(s) 81, 160, 411
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 307, 352
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 2 cut(s) 80, 414
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 2 cut(s) 405, 516
Bsp143I GATC 4 cut(s) 66, 151, 463, 472
BspACI CCGC 1 cut(s) 228
BspANI GGCC 2 cut(s) 80, 414
BspCNI CTCAG 2 cut(s) 367, 502
BspPI GGATC 2 cut(s) 458, 467
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 4 cut(s) 66, 151, 463, 472
Bst2UI CCWGG 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 354, 510
BstEII GGTNACC 1 cut(s) 171
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 4 cut(s) 69, 154, 466, 475
BstMAI GTCTC 2 cut(s) 307, 352
BstMBI GATC 4 cut(s) 66, 151, 463, 472
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 2 cut(s) 71, 92
BstNI CCWGG 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 405
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 2 cut(s) 80, 414
BtgZI GCGATG 1 cut(s) 72
BtsI GCAGTG 2 cut(s) 81, 453
BtsIMutI CAGTG 2 cut(s) 81, 453
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 264, 289
CviJI RGCY 8 cut(s) 39, 80, 95, 256, 408, 414, 514, 520
CviKI_1 RGCY 8 cut(s) 39, 80, 95, 256, 408, 414, 514, 520
CviQI GTAC 2 cut(s) 264, 289
DdeI CTNAG 2 cut(s) 354, 510
DpnI GATC 4 cut(s) 68, 153, 465, 474
DpnII GATC 4 cut(s) 66, 151, 463, 472
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 514
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 307
FaiI YATR 1 cut(s) 168
FaqI GGGAC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 229
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 1 cut(s) 229
GlaI GCGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 229
GsaI CCCAGC 2 cut(s) 256, 408
HaeIII GGCC 2 cut(s) 80, 414
HapII CCGG 3 cut(s) 81, 160, 411
HhaI GCGC 1 cut(s) 74
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 6 cut(s) 221, 310, 393, 536, 542, 566
HpaII CCGG 3 cut(s) 81, 160, 411
HphI GGTGA 2 cut(s) 146, 391
Hpy188I TCNGA 5 cut(s) 125, 392, 434, 511, 541
Hpy188III TCNNGA 2 cut(s) 356, 461
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 495
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 92
HpyF3I CTNAG 2 cut(s) 354, 510
HpySE526I ACGT 1 cut(s) 300
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 4 cut(s) 66, 151, 463, 472
LmnI GCTCC 3 cut(s) 57, 83, 413
LweI GCATC 5 cut(s) 52, 95, 129, 153, 207
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 107, 171, 436
MalI GATC 4 cut(s) 68, 153, 465, 474
MboI GATC 4 cut(s) 66, 151, 463, 472
MboII GAAGA 5 cut(s) 38, 355, 480, 483, 486
MhlI GDGCHC 2 cut(s) 405, 516
MluCI AATT 6 cut(s) 233, 327, 499, 547, 553, 560
MlyI GAGTC 2 cut(s) 304, 402
MmeI TCCRAC 1 cut(s) 412
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 3 cut(s) 81, 160, 411
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 71, 92
NdeII GATC 4 cut(s) 66, 151, 463, 472
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 4 cut(s) 221, 536, 542, 566
PkrI GCNGC 1 cut(s) 230
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 2 cut(s) 304, 401
PpsI GAGTC 2 cut(s) 304, 401
Psp124BI GAGCTC 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 171
PspFI CCCAGC 2 cut(s) 252, 404
PspGI CCWGG 1 cut(s) 237
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 2 cut(s) 265, 290
RsaNI GTAC 2 cut(s) 264, 289
SacI GAGCTC 1 cut(s) 516
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 4 cut(s) 66, 151, 463, 472
SchI GAGTC 2 cut(s) 304, 402
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 405, 516
SetI ASST 9 cut(s) 159, 186, 303, 325, 388, 410, 516, 522, 528
SfaNI GCATC 5 cut(s) 52, 95, 129, 153, 207
SmlI CTYRAG 2 cut(s) 515, 521
SmoI CTYRAG 2 cut(s) 515, 521
Sse9I AATT 6 cut(s) 233, 327, 499, 547, 553, 560
SsiI CCGC 1 cut(s) 228
SspI AATATT 1 cut(s) 209
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 237
TaiI ACGT 1 cut(s) 303
TaqI TCGA 5 cut(s) 180, 216, 325, 396, 564
TasI AATT 6 cut(s) 233, 327, 499, 547, 553, 560
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 4 cut(s) 221, 536, 542, 566
TscAI CASTG 2 cut(s) 81, 460
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 81, 460
XapI RAATTY 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.