RchiOBHm_Chr6g0282751

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000944
Physical Location & Seq
Forward (+)
45970689 .. 45972789
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25351

Sequence Viewer

Length: 570 bp
ATGTTGCTACTACAGTTCACTTCCAACTCCATACCCTTTCTCTGCTTCCTCTTACTGCTCCCGATCATGACCATTAGCTCCGATTCGTCCGTCTACGACGTCCTCCTGGCCCACGGCCTTCCGATGGGGCTGCTGCCTAAGGGTGTGACTCAGTTCGAAATCGACGAGGGCGGCCGGTTCCAGGTGTACCTGGATCAGGCCTGCAATGCCAAGTTCGAGAGTGAGTTGCATTACGACAGGAACGTGTCTGGGACTCTCAGTTACGGCCGGATCGGAGCCTTGTCGGGTATTTCGGCCCAGGAGCTCTTTCTCTGGTTTCCGGTCAAGGGTATTCGGGTCGATGTCCCCAGCTCCGGCATCATTTACTTCGATGTCGGCGTTGTTTTTAAGCAATTCTCGCTTTCGCTATTCGAAACACCGCCGGATTGTACTGCTGCCACCCCCGCCGCCCAGGCTGATGTCGGCGGCGGCGGCGGCAGGCTCATCGTTGACACCGTTTCCAAGAGTAAATCGGGCAAACTGCGGTATGGTCTCGATCAGGAGCAACATTTTGGGAGAGGTTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

189

Amino Acids

20.54

Weight (kDa)

5.15

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 31 - 138 2.6e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 93
AciI CCGC 9 cut(s) 171, 419, 444, 447, 465, 468, 471, 474, 523
AclWI GGATC 2 cut(s) 201, 278
AcoI YGGCCR 2 cut(s) 172, 265
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 188, 430
AfiI CCNNNNNNNGG 5 cut(s) 124, 181, 196, 326, 353
AflIII ACRYGT 1 cut(s) 243
AjnI CCWGG 5 cut(s) 105, 180, 189, 297, 450
AluBI AGCT 3 cut(s) 78, 304, 351
AluI AGCT 3 cut(s) 78, 304, 351
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 2 cut(s) 201, 278
AoxI GGCC 6 cut(s) 108, 115, 172, 198, 265, 294
ApeKI GCWGC 3 cut(s) 130, 133, 434
AspS9I GGNCC 2 cut(s) 109, 295
AsuII TTCGAA 2 cut(s) 156, 411
AxyI CCTNAGG 1 cut(s) 138
BanII GRGCYC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 117, 120, 421
BccI CCATC 1 cut(s) 118
BceAI ACGGC 2 cut(s) 130, 280
BcgI CGANNNNNNTGC 4 cut(s) 112, 146, 466, 500
BciT130I CCWGG 5 cut(s) 107, 182, 191, 299, 452
BcoDI GTCTC 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 11
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 9 cut(s) 131, 134, 172, 435, 447, 466, 469, 472, 475
BlsI GCNGC 9 cut(s) 132, 135, 173, 436, 448, 467, 470, 473, 476
Bme1390I CCNGG 5 cut(s) 107, 182, 191, 299, 452
BmgT120I GGNCC 2 cut(s) 109, 295
BmiI GGNNCC 2 cut(s) 179, 277
BmrFI CCNGG 5 cut(s) 107, 182, 191, 299, 452
BmsI GCATC 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 156, 411
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 3 cut(s) 112, 297, 450
BsaWI WCCGGW 1 cut(s) 319
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 181, 196, 326, 353
Bse118I RCCGGY 1 cut(s) 174
Bse21I CCTNAGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 5 cut(s) 107, 182, 191, 299, 452
BseDI CCNNGG 3 cut(s) 112, 297, 450
BseLI CCNNNNNNNGG 5 cut(s) 124, 181, 196, 326, 353
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 164, 271
BseX3I CGGCCG 2 cut(s) 172, 265
BseXI GCAGC 3 cut(s) 117, 120, 421
BseYI CCCAGC 1 cut(s) 347
Bsh1285I CGRYCG 2 cut(s) 175, 268
BshFI GGCC 6 cut(s) 110, 117, 174, 200, 267, 296
BsiEI CGRYCG 2 cut(s) 175, 268
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 5 cut(s) 175, 268, 320, 354, 422
BslFI GGGAC 2 cut(s) 265, 329
BslI CCNNNNNNNGG 5 cut(s) 124, 181, 196, 326, 353
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 2 cut(s) 265, 329
BsnI GGCC 6 cut(s) 110, 117, 174, 200, 267, 296
Bso31I GGTCTC 1 cut(s) 536
Bsp119I TTCGAA 2 cut(s) 156, 411
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 4 cut(s) 63, 193, 270, 535
BspACI CCGC 9 cut(s) 171, 419, 444, 447, 465, 468, 471, 474, 523
BspANI GGCC 6 cut(s) 110, 117, 174, 200, 267, 296
BspCNI CTCAG 2 cut(s) 163, 270
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 179, 277
BspPI GGATC 2 cut(s) 201, 278
BspT104I TTCGAA 2 cut(s) 156, 411
BspTNI GGTCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 3 cut(s) 112, 297, 450
BssMI GATC 4 cut(s) 63, 193, 270, 535
BssNI GRCGYC 1 cut(s) 99
Bst2UI CCWGG 5 cut(s) 107, 182, 191, 299, 452
Bst4CI ACNGT 2 cut(s) 15, 496
BstACI GRCGYC 1 cut(s) 99
BstBI TTCGAA 2 cut(s) 156, 411
BstC8I GCNNGC 2 cut(s) 202, 479
BstDEI CTNAG 3 cut(s) 138, 150, 257
BstDSI CCRYGG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 194
BstKTI GATC 4 cut(s) 66, 196, 273, 538
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 4 cut(s) 63, 193, 270, 535
BstMCI CGRYCG 2 cut(s) 175, 268
BstMWI GCNNNNNNNGC 6 cut(s) 206, 397, 443, 452, 471, 474
BstNI CCWGG 5 cut(s) 107, 182, 191, 299, 452
BstSCI CCNGG 5 cut(s) 105, 180, 189, 297, 450
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 117, 120, 421
BstZI CGGCCG 2 cut(s) 172, 265
Bsu36I CCTNAGG 1 cut(s) 138
BsuRI GGCC 6 cut(s) 110, 117, 174, 200, 267, 296
BtgI CCRYGG 1 cut(s) 112
Cac8I GCNNGC 2 cut(s) 202, 479
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 174
Cfr13I GGNCC 2 cut(s) 109, 295
Csp6I GTAC 2 cut(s) 187, 429
CviAII CATG 1 cut(s) 67
CviQI GTAC 2 cut(s) 187, 429
DdeI CTNAG 3 cut(s) 138, 150, 257
DpnI GATC 4 cut(s) 65, 195, 272, 537
DpnII GATC 4 cut(s) 63, 193, 270, 535
EaeI YGGCCR 2 cut(s) 172, 265
EagI CGGCCG 2 cut(s) 172, 265
Ecl136II GAGCTC 1 cut(s) 304
EclXI CGGCCG 2 cut(s) 172, 265
Eco147I AGGCCT 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 536
Eco52I CGGCCG 2 cut(s) 172, 265
Eco53kI GAGCTC 1 cut(s) 304
Eco81I CCTNAGG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoRII CCWGG 5 cut(s) 105, 180, 189, 297, 450
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 1 cut(s) 70
FaiI YATR 3 cut(s) 32, 68, 528
FaqI GGGAC 2 cut(s) 265, 329
FatI CATG 1 cut(s) 66
FauI CCCGC 1 cut(s) 451
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 9 cut(s) 131, 134, 172, 435, 447, 466, 469, 472, 475
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 9 cut(s) 131, 134, 172, 435, 447, 466, 469, 472, 475
GluI GCNGC 9 cut(s) 131, 134, 172, 435, 447, 466, 469, 472, 475
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 6 cut(s) 110, 117, 174, 200, 267, 296
HapII CCGG 5 cut(s) 175, 268, 320, 354, 422
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 1 cut(s) 70
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 3 cut(s) 83, 148, 253
HpaII CCGG 5 cut(s) 175, 268, 320, 354, 422
Hpy166II GTNNAC 4 cut(s) 18, 94, 187, 490
Hpy188I TCNGA 3 cut(s) 82, 123, 275
Hpy188III TCNNGA 5 cut(s) 61, 67, 217, 533, 539
Hpy8I GTNNAC 4 cut(s) 18, 94, 187, 490
Hpy99I CGWCG 2 cut(s) 101, 167
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 15, 496
HpyCH4IV ACGT 2 cut(s) 99, 243
HpyCH4V TGCA 2 cut(s) 204, 229
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 397, 443, 452, 471, 474
HpyF3I CTNAG 3 cut(s) 138, 150, 257
HpySE526I ACGT 2 cut(s) 99, 243
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 1 cut(s) 70
Kzo9I GATC 4 cut(s) 63, 193, 270, 535
LmnI GCTCC 6 cut(s) 63, 83, 275, 301, 356, 541
Lsp1109I GCAGC 3 cut(s) 117, 120, 421
LweI GCATC 1 cut(s) 366
MaeII ACGT 2 cut(s) 99, 243
MaeIII GTNAC 2 cut(s) 145, 260
MalI GATC 4 cut(s) 65, 195, 272, 537
MboI GATC 4 cut(s) 63, 193, 270, 535
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 1 cut(s) 392
MlyI GAGTC 2 cut(s) 142, 247
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 4 cut(s) 59, 113, 160, 551
MseI TTAA 1 cut(s) 387
MspI CCGG 5 cut(s) 175, 268, 320, 354, 422
MspR9I CCNGG 5 cut(s) 107, 182, 191, 299, 452
MvaI CCWGG 5 cut(s) 107, 182, 191, 299, 452
MwoI GCNNNNNNNGC 6 cut(s) 206, 397, 443, 452, 471, 474
NdeII GATC 4 cut(s) 63, 193, 270, 535
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 2 cut(s) 179, 277
NmuCI GTSAC 1 cut(s) 145
NspV TTCGAA 2 cut(s) 156, 411
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 200
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 240, 375, 492
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 9 cut(s) 132, 135, 173, 436, 448, 467, 470, 473, 476
PleI GAGTC 2 cut(s) 142, 247
PpsI GAGTC 2 cut(s) 142, 247
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 5 cut(s) 105, 180, 189, 297, 450
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 5 cut(s) 105, 180, 189, 297, 450
PspN4I GGNNCC 2 cut(s) 179, 277
PspPI GGNCC 2 cut(s) 109, 295
RsaI GTAC 2 cut(s) 188, 430
RsaNI GTAC 2 cut(s) 187, 429
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 9 cut(s) 131, 134, 172, 435, 447, 466, 469, 472, 475
Sau3AI GATC 4 cut(s) 63, 193, 270, 535
Sau96I GGNCC 2 cut(s) 109, 295
SchI GAGTC 2 cut(s) 142, 247
ScrFI CCNGG 5 cut(s) 107, 182, 191, 299, 452
SduI GDGCHC 1 cut(s) 306
SetI ASST 8 cut(s) 80, 102, 186, 192, 246, 306, 353, 562
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 1 cut(s) 11
SfuI TTCGAA 2 cut(s) 156, 411
Sse9I AATT 1 cut(s) 392
SseBI AGGCCT 1 cut(s) 200
SsiI CCGC 9 cut(s) 171, 419, 444, 447, 465, 468, 471, 474, 523
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 5 cut(s) 105, 180, 189, 297, 450
TaaI ACNGT 2 cut(s) 15, 496
TaiI ACGT 2 cut(s) 102, 246
TaqI TCGA 7 cut(s) 156, 162, 216, 339, 369, 411, 534
TasI AATT 1 cut(s) 392
TatI WGTACW 1 cut(s) 428
TauI GCSGC 6 cut(s) 174, 449, 468, 471, 474, 477
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 3 cut(s) 130, 133, 434
Tsp45I GTSAC 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 79
XagI CCTNNNNNAGG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 93
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.