Rh6BG265200

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000944
Physical Location & Seq
Forward (+)
48040082 .. 48042725
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG265200.1

Sequence Viewer

Length: 639 bp
ATGATATATAAACACAACACCAAAATTCACTTCACTGATCACTCTCCCAACCTCACTCCAATCCCAAACACCATGTTGCTACTACAGTTCACTTCCAACTCCATACCCTTTCTCTGCTTCCTCTTATTGCTCCCGATCATGACCATTAGCTCCGATTCGTCGGTCTACGACGTCCTTCTGGCCCACGGCCTTCCTATGGGGCTGCTGCCTAAGGGTGTGACTCAGTTCGAAATCGACGAGGGCGGCCGGTTCCAGGTGTACCTGGATCAGGCCTGCAATGCCAAGTTCGAGAGTGAGTTGCATTACGACAGGAACGTGTCTGGGACTCTCAGTTACGGCCGGATCGGAGCCTTGTCGGGTATTTCGGCCCAGGAGCTCTTTCTCTGGTTTCCGGTCAAGGGTATTCGGGTCGATGTCCCCAGCTCCGGCATCATTTACTTCGATGTCGGCGTTGTTTTTAAGCAATTCTCGCTTTCGCTATTCGAAACACCGCCGGATTGTACTGCTGCCACCCCCGCCGCCCAGGCTGATGTCGACGGCGGCGGCAGGCTCATCGTTGACACCGTTTCCAAGAGTAAATCGGGCAAACTGCGGTATGGTCTCGATCAGGAGCAACATTTTGGGAGAGGTTTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

212

Amino Acids

23.34

Weight (kDa)

5.64

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 55 - 162 3.5e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccI GTMKAC 2 cut(s) 165, 534
AciI CCGC 7 cut(s) 243, 491, 516, 519, 540, 543, 592
AclWI GGATC 2 cut(s) 273, 350
AcoI YGGCCR 2 cut(s) 244, 337
AcsI RAATTY 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 260, 502
AfiI CCNNNNNNNGG 5 cut(s) 196, 253, 268, 398, 425
AflIII ACRYGT 1 cut(s) 315
AjnI CCWGG 4 cut(s) 252, 261, 369, 522
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 3 cut(s) 150, 376, 423
AluI AGCT 3 cut(s) 150, 376, 423
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 605
AlwI GGATC 2 cut(s) 273, 350
AoxI GGCC 6 cut(s) 180, 187, 244, 270, 337, 366
ApeKI GCWGC 3 cut(s) 202, 205, 506
ApoI RAATTY 1 cut(s) 24
AspS9I GGNCC 2 cut(s) 181, 367
AsuII TTCGAA 2 cut(s) 228, 483
AxyI CCTNAGG 1 cut(s) 210
BanII GRGCYC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 189, 192, 493
BceAI ACGGC 3 cut(s) 202, 352, 553
BcgI CGANNNNNNTGC 2 cut(s) 535, 569
BciT130I CCWGG 4 cut(s) 254, 263, 371, 524
BclI TGATCA 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 605
BfmI CTRYAG 1 cut(s) 83
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 7 cut(s) 203, 206, 244, 507, 519, 541, 544
BlsI GCNGC 7 cut(s) 204, 207, 245, 508, 520, 542, 545
Bme1390I CCNGG 4 cut(s) 254, 263, 371, 524
BmgT120I GGNCC 2 cut(s) 181, 367
BmiI GGNNCC 2 cut(s) 251, 349
BmrFI CCNGG 4 cut(s) 254, 263, 371, 524
BmsI GCATC 1 cut(s) 438
Bpu14I TTCGAA 2 cut(s) 228, 483
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 184, 369, 522
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 5 cut(s) 196, 253, 268, 398, 425
Bse118I RCCGGY 1 cut(s) 246
Bse21I CCTNAGG 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 4 cut(s) 254, 263, 371, 524
BseDI CCNNGG 3 cut(s) 184, 369, 522
BseLI CCNNNNNNNGG 5 cut(s) 196, 253, 268, 398, 425
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 236, 343
BseX3I CGGCCG 2 cut(s) 244, 337
BseXI GCAGC 3 cut(s) 189, 192, 493
BseYI CCCAGC 1 cut(s) 419
Bsh1285I CGRYCG 2 cut(s) 247, 340
BshFI GGCC 6 cut(s) 182, 189, 246, 272, 339, 368
BsiEI CGRYCG 2 cut(s) 247, 340
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 5 cut(s) 247, 340, 392, 426, 494
BslFI GGGAC 2 cut(s) 337, 401
BslI CCNNNNNNNGG 5 cut(s) 196, 253, 268, 398, 425
BsmAI GTCTC 1 cut(s) 605
BsmFI GGGAC 2 cut(s) 337, 401
BsnI GGCC 6 cut(s) 182, 189, 246, 272, 339, 368
Bso31I GGTCTC 1 cut(s) 605
Bsp119I TTCGAA 2 cut(s) 228, 483
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 5 cut(s) 37, 135, 265, 342, 604
BspACI CCGC 7 cut(s) 243, 491, 516, 519, 540, 543, 592
BspANI GGCC 6 cut(s) 182, 189, 246, 272, 339, 368
BspCNI CTCAG 2 cut(s) 235, 342
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 251, 349
BspPI GGATC 2 cut(s) 273, 350
BspT104I TTCGAA 2 cut(s) 228, 483
BspTNI GGTCTC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 246
BssECI CCNNGG 3 cut(s) 184, 369, 522
BssMI GATC 5 cut(s) 37, 135, 265, 342, 604
BssNI GRCGYC 1 cut(s) 171
Bst2UI CCWGG 4 cut(s) 254, 263, 371, 524
Bst4CI ACNGT 2 cut(s) 87, 565
BstACI GRCGYC 1 cut(s) 171
BstBI TTCGAA 2 cut(s) 228, 483
BstC8I GCNNGC 2 cut(s) 274, 548
BstDEI CTNAG 3 cut(s) 210, 222, 329
BstDSI CCRYGG 1 cut(s) 184
BstENI CCTNNNNNAGG 1 cut(s) 266
BstKTI GATC 5 cut(s) 40, 138, 268, 345, 607
BstMAI GTCTC 1 cut(s) 605
BstMBI GATC 5 cut(s) 37, 135, 265, 342, 604
BstMCI CGRYCG 2 cut(s) 247, 340
BstMWI GCNNNNNNNGC 4 cut(s) 278, 469, 515, 524
BstNI CCWGG 4 cut(s) 254, 263, 371, 524
BstSCI CCNGG 4 cut(s) 252, 261, 369, 522
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 189, 192, 493
BstZI CGGCCG 2 cut(s) 244, 337
Bsu36I CCTNAGG 1 cut(s) 210
BsuRI GGCC 6 cut(s) 182, 189, 246, 272, 339, 368
BtgI CCRYGG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 2 cut(s) 274, 548
CciI TCATGA 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 181, 367
Csp6I GTAC 2 cut(s) 259, 501
CviAII CATG 2 cut(s) 73, 139
CviQI GTAC 2 cut(s) 259, 501
DdeI CTNAG 3 cut(s) 210, 222, 329
DpnI GATC 5 cut(s) 39, 137, 267, 344, 606
DpnII GATC 5 cut(s) 37, 135, 265, 342, 604
EaeI YGGCCR 2 cut(s) 244, 337
EagI CGGCCG 2 cut(s) 244, 337
Ecl136II GAGCTC 1 cut(s) 376
EclXI CGGCCG 2 cut(s) 244, 337
Eco147I AGGCCT 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 605
Eco52I CGGCCG 2 cut(s) 244, 337
Eco53kI GAGCTC 1 cut(s) 376
Eco81I CCTNAGG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRII CCWGG 4 cut(s) 252, 261, 369, 522
EcoT38I GRGCYC 1 cut(s) 378
FaeI CATG 2 cut(s) 76, 142
FaiI YATR 7 cut(s) 7, 9, 74, 104, 140, 197, 597
FaqI GGGAC 2 cut(s) 337, 401
FatI CATG 2 cut(s) 72, 138
FauI CCCGC 1 cut(s) 523
FbaI TGATCA 1 cut(s) 37
FblI GTMKAC 2 cut(s) 165, 534
Fnu4HI GCNGC 7 cut(s) 203, 206, 244, 507, 519, 541, 544
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 7 cut(s) 203, 206, 244, 507, 519, 541, 544
GluI GCNGC 7 cut(s) 203, 206, 244, 507, 519, 541, 544
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 6 cut(s) 182, 189, 246, 272, 339, 368
HapII CCGG 5 cut(s) 247, 340, 392, 426, 494
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 2 cut(s) 76, 142
HincII GTYRAC 2 cut(s) 535, 559
HindII GTYRAC 2 cut(s) 535, 559
HinfI GANTC 3 cut(s) 155, 220, 325
HpaII CCGG 5 cut(s) 247, 340, 392, 426, 494
Hpy166II GTNNAC 5 cut(s) 90, 166, 259, 535, 559
Hpy188I TCNGA 2 cut(s) 154, 347
Hpy188III TCNNGA 5 cut(s) 133, 139, 289, 602, 608
Hpy8I GTNNAC 5 cut(s) 90, 166, 259, 535, 559
Hpy99I CGWCG 4 cut(s) 163, 173, 239, 539
HpyAV CCTTC 2 cut(s) 185, 200
HpyCH4III ACNGT 2 cut(s) 87, 565
HpyCH4IV ACGT 2 cut(s) 171, 315
HpyCH4V TGCA 2 cut(s) 276, 301
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 469, 515, 524
HpyF3I CTNAG 3 cut(s) 210, 222, 329
HpySE526I ACGT 2 cut(s) 171, 315
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 2 cut(s) 76, 142
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 5 cut(s) 37, 135, 265, 342, 604
LmnI GCTCC 6 cut(s) 135, 155, 347, 373, 428, 610
Lsp1109I GCAGC 3 cut(s) 189, 192, 493
LweI GCATC 1 cut(s) 438
MaeII ACGT 2 cut(s) 171, 315
MaeIII GTNAC 2 cut(s) 217, 332
MalI GATC 5 cut(s) 39, 137, 267, 344, 606
MboI GATC 5 cut(s) 37, 135, 265, 342, 604
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 2 cut(s) 24, 464
MlyI GAGTC 2 cut(s) 214, 319
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 62, 131, 232, 620
MseI TTAA 1 cut(s) 459
MspI CCGG 5 cut(s) 247, 340, 392, 426, 494
MspR9I CCNGG 4 cut(s) 254, 263, 371, 524
MvaI CCWGG 4 cut(s) 254, 263, 371, 524
MwoI GCNNNNNNNGC 4 cut(s) 278, 469, 515, 524
NdeII GATC 5 cut(s) 37, 135, 265, 342, 604
NlaIII CATG 2 cut(s) 76, 142
NlaIV GGNNCC 2 cut(s) 251, 349
NmuCI GTSAC 1 cut(s) 217
NspV TTCGAA 2 cut(s) 228, 483
PagI TCATGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 272
PcsI WCGNNNNNNNCGW 4 cut(s) 234, 312, 447, 561
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 7 cut(s) 204, 207, 245, 508, 520, 542, 545
PleI GAGTC 2 cut(s) 214, 319
PpsI GAGTC 2 cut(s) 214, 319
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 4 cut(s) 252, 261, 369, 522
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 4 cut(s) 252, 261, 369, 522
PspN4I GGNNCC 2 cut(s) 251, 349
PspPI GGNCC 2 cut(s) 181, 367
RsaI GTAC 2 cut(s) 260, 502
RsaNI GTAC 2 cut(s) 259, 501
SacI GAGCTC 1 cut(s) 378
SalI GTCGAC 1 cut(s) 533
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 7 cut(s) 203, 206, 244, 507, 519, 541, 544
Sau3AI GATC 5 cut(s) 37, 135, 265, 342, 604
Sau96I GGNCC 2 cut(s) 181, 367
SchI GAGTC 2 cut(s) 214, 319
ScrFI CCNGG 4 cut(s) 254, 263, 371, 524
SduI GDGCHC 1 cut(s) 378
SetI ASST 9 cut(s) 54, 152, 174, 258, 264, 318, 378, 425, 631
SfaNI GCATC 1 cut(s) 438
SfcI CTRYAG 1 cut(s) 83
SfuI TTCGAA 2 cut(s) 228, 483
Sse9I AATT 2 cut(s) 24, 464
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 7 cut(s) 243, 491, 516, 519, 540, 543, 592
SstI GAGCTC 1 cut(s) 378
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 4 cut(s) 252, 261, 369, 522
TaaI ACNGT 2 cut(s) 87, 565
TaiI ACGT 2 cut(s) 174, 318
TaqI TCGA 8 cut(s) 228, 234, 288, 411, 441, 483, 534, 603
TaqII GACCGA 1 cut(s) 151
TasI AATT 2 cut(s) 24, 464
TatI WGTACW 1 cut(s) 500
TauI GCSGC 4 cut(s) 246, 521, 543, 546
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 3 cut(s) 202, 205, 506
Tsp45I GTSAC 1 cut(s) 217
TspRI CASTG 1 cut(s) 40
XagI CCTNNNNNAGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 24
XmiI GTMKAC 2 cut(s) 165, 534
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.