RLG00000033948

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000944
Physical Location & Seq
Reverse (-)
34605395 .. 34606012
618 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033948

Sequence Viewer

Length: 618 bp
ATGGCACTGGGATTGCAAGGTTCAAAATCCCTAGGGGGCTTTTTTCTTCTGCTCTTCCTTGCTGCGATCGACGCCATGGCCGGAGCATCGCCGGCCCCAAAATCCAGTAACAAAACAAGCATCGAAGAAGGAGGAAAAGGAAGCATCTACGATCACCTCCGGCAATATGGGTTACCCATCGACCACCTCCCCAAAGGCATTACGGAATATTCCCTCGACGATTCTAGTGGCGAATTCCTGGTGTTTCTGCCCCAGCCTTGCCGCCCCAAGTTCGAGAATCAGGTCCGGTACGATTTCAACGTCTCTGGGACTCTCACTTTCGGCCGAATCGTCTACTTATCCGGCGTCTCTGCTCAGGAACTCTTCCTCTGGTTTCCGGTGAAAGGTATCCGAGTCGATGTGCCCAGCTCCGGCCTCATTTACTTCAATGTCGGCGTTGTCGATAAGCAGTTCTCTTTGTCCCTCTTCGAGTCTCCGCCCGATTGCACTGCTCTTGATCCTTCTGATCCCTCCTCTCCTTCTTCTTCTTCCAATTCCCCATCCGAGCTCAAGCTCAAGGTAGAATGTGATTCTGATTCCAATTCCAATTCTCAATTCGATTTCGTTGCTTCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

206

Amino Acids

22.11

Weight (kDa)

4.68

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 49 - 157 3.7e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 333
AciI CCGC 2 cut(s) 262, 476
AclWI GGATC 2 cut(s) 491, 500
AcoI YGGCCR 2 cut(s) 78, 322
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 2 cut(s) 72, 345
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 2 cut(s) 383, 410
AgsI TTSAA 3 cut(s) 24, 298, 427
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 3 cut(s) 408, 547, 553
AluI AGCT 3 cut(s) 408, 547, 553
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 3 cut(s) 307, 352, 477
AlwI GGATC 2 cut(s) 491, 500
AoxI GGCC 4 cut(s) 78, 93, 322, 412
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 1 cut(s) 233
AspA2I CCTAGG 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 94, 283
AsuHPI GGTGA 2 cut(s) 146, 391
AvaII GGWCC 1 cut(s) 283
AvrII CCTAGG 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 49
BccI CCATC 2 cut(s) 185, 547
BcgI CGANNNNNNTGC 3 cut(s) 470, 504, 587
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 398
BcoDI GTCTC 3 cut(s) 307, 352, 477
BfaI CTAG 2 cut(s) 32, 225
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 2 cut(s) 63, 262
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 2 cut(s) 64, 263
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 94, 283
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 239
BmrI ACTGGG 1 cut(s) 17
BmsI GCATC 3 cut(s) 95, 129, 153
BmuI ACTGGG 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 533, 539
BsaHI GRCGYC 2 cut(s) 72, 345
BsaJI CCNNGG 2 cut(s) 31, 75
BsaWI WCCGGW 2 cut(s) 285, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 410
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 12, 105
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 31, 75
BseGI GGATG 1 cut(s) 539
BseLI CCNNNNNNNGG 2 cut(s) 383, 410
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 2 cut(s) 12, 105
BseRI GAGGAG 1 cut(s) 502
BseSI GKGCMC 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 322
BseXI GCAGC 1 cut(s) 49
BseYI CCCAGC 2 cut(s) 252, 404
Bsh1285I CGRYCG 2 cut(s) 69, 325
BshFI GGCC 4 cut(s) 80, 95, 324, 414
BsiEI CGRYCG 2 cut(s) 69, 325
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 7 cut(s) 81, 92, 160, 286, 342, 377, 411
BslFI GGGAC 2 cut(s) 322, 445
BslI CCNNNNNNNGG 2 cut(s) 383, 410
BsmAI GTCTC 3 cut(s) 307, 352, 477
BsmBI CGTCTC 2 cut(s) 307, 352
BsmFI GGGAC 2 cut(s) 322, 445
BsnI GGCC 4 cut(s) 80, 95, 324, 414
Bsp1286I GDGCHC 2 cut(s) 405, 549
Bsp143I GATC 4 cut(s) 66, 151, 496, 505
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 2 cut(s) 262, 476
BspANI GGCC 4 cut(s) 80, 95, 324, 414
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 2 cut(s) 491, 500
BspQI GCTCTTC 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 12, 105
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 31, 75
BssMI GATC 4 cut(s) 66, 151, 496, 505
BssNI GRCGYC 2 cut(s) 72, 345
BssT1I CCWWGG 2 cut(s) 31, 75
Bst2UI CCWGG 1 cut(s) 239
Bst6I CTCTTC 3 cut(s) 59, 368, 470
BstACI GRCGYC 2 cut(s) 72, 345
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 75
BstEII GGTNACC 1 cut(s) 171
BstF5I GGATG 1 cut(s) 539
BstKTI GATC 4 cut(s) 69, 154, 499, 508
BstMAI GTCTC 3 cut(s) 307, 352, 477
BstMBI GATC 4 cut(s) 66, 151, 496, 505
BstMCI CGRYCG 2 cut(s) 69, 325
BstMWI GCNNNNNNNGC 2 cut(s) 71, 92
BstNI CCWGG 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 49
BstZI CGGCCG 1 cut(s) 322
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 4 cut(s) 80, 95, 324, 414
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 539
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 5, 486
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 94, 283
CseI GACGC 2 cut(s) 80, 334
Csp6I GTAC 1 cut(s) 289
CviAII CATG 1 cut(s) 76
CviJI RGCY 9 cut(s) 39, 80, 95, 256, 324, 408, 414, 547, 553
CviKI_1 RGCY 9 cut(s) 39, 80, 95, 256, 324, 408, 414, 547, 553
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 354
DpnI GATC 4 cut(s) 68, 153, 498, 507
DpnII GATC 4 cut(s) 66, 151, 496, 505
EaeI YGGCCR 2 cut(s) 78, 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 3 cut(s) 59, 368, 470
EarI CTCTTC 3 cut(s) 59, 368, 470
EciI GGCGGA 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 547
EclXI CGGCCG 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 31, 75
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 283
Eco52I CGGCCG 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 547
Eco91I GGTNACC 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 171
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 31, 75
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 2 cut(s) 31, 75
Esp3I CGTCTC 2 cut(s) 307, 352
FaeI CATG 1 cut(s) 79
FaiI YATR 2 cut(s) 77, 168
FaqI GGGAC 2 cut(s) 322, 445
FatI CATG 1 cut(s) 75
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 63, 262
FokI GGATG 1 cut(s) 526
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 2 cut(s) 63, 262
FspBI CTAG 2 cut(s) 32, 225
GluI GCNGC 2 cut(s) 63, 262
GsaI CCCAGC 2 cut(s) 256, 408
HaeIII GGCC 4 cut(s) 80, 95, 324, 414
HapII CCGG 7 cut(s) 81, 92, 160, 286, 342, 377, 411
HgaI GACGC 2 cut(s) 80, 334
Hin1I GRCGYC 2 cut(s) 72, 345
Hin1II CATG 1 cut(s) 79
HinfI GANTC 8 cut(s) 221, 277, 310, 327, 393, 470, 569, 575
HpaII CCGG 7 cut(s) 81, 92, 160, 286, 342, 377, 411
HphI GGTGA 2 cut(s) 146, 391
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 392, 505, 544, 574
Hpy188III TCNNGA 3 cut(s) 274, 356, 494
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 2 cut(s) 74, 221
HpyAV CCTTC 3 cut(s) 122, 510, 528
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 16, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 92
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 2 cut(s) 72, 345
Hsp92II CATG 1 cut(s) 79
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 4 cut(s) 66, 151, 496, 505
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 2 cut(s) 83, 413
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 3 cut(s) 95, 129, 153
MaeI CTAG 2 cut(s) 32, 225
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 107, 171
MalI GATC 4 cut(s) 68, 153, 498, 507
MboI GATC 4 cut(s) 66, 151, 496, 505
MboII GAAGA 8 cut(s) 38, 46, 137, 355, 457, 513, 516, 519
MhlI GDGCHC 2 cut(s) 405, 549
MluCI AATT 5 cut(s) 233, 532, 580, 586, 593
MlyI GAGTC 3 cut(s) 304, 402, 479
MnlI CCTC 9 cut(s) 125, 167, 197, 224, 377, 425, 473, 520, 523
MroNI GCCGGC 1 cut(s) 91
MspI CCGG 7 cut(s) 81, 92, 160, 286, 342, 377, 411
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 71, 92
NaeI GCCGGC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 75
NdeII GATC 4 cut(s) 66, 151, 496, 505
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 96
PciSI GCTCTTC 1 cut(s) 59
PcsI WCGNNNNNNNCGW 3 cut(s) 297, 327, 438
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 5 cut(s) 221, 277, 327, 569, 575
PkrI GCNGC 2 cut(s) 64, 263
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 3 cut(s) 304, 401, 478
PpsI GAGTC 3 cut(s) 304, 401, 478
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 171
PspFI CCCAGC 2 cut(s) 252, 404
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 94, 283
PvuI CGATCG 1 cut(s) 69
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 59
SatI GCNGC 2 cut(s) 63, 262
Sau3AI GATC 4 cut(s) 66, 151, 496, 505
Sau96I GGNCC 2 cut(s) 94, 283
SchI GAGTC 3 cut(s) 304, 402, 479
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 405, 549
SfaNI GCATC 3 cut(s) 95, 129, 153
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 2 cut(s) 548, 554
SmoI CTYRAG 2 cut(s) 548, 554
Sse9I AATT 5 cut(s) 233, 532, 580, 586, 593
SsiI CCGC 2 cut(s) 262, 476
SspI AATATT 1 cut(s) 209
SspMI CTAG 2 cut(s) 32, 225
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 2 cut(s) 31, 75
TaiI ACGT 1 cut(s) 303
TasI AATT 5 cut(s) 233, 532, 580, 586, 593
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 5 cut(s) 221, 277, 327, 569, 575
TscAI CASTG 2 cut(s) 12, 493
TseI GCWGC 1 cut(s) 62
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 12, 493
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 32, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.