pycom10g13050

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000944
Physical Location & Seq
Forward (+)
16524640 .. 16526612
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g13050.4

Sequence Viewer

Length: 1059 bp
ATGCCCATGATGGCTGATCTATGCCCACAACAGTCAGGGGGTGGAGGTTTATGGATATTGTCGGAAGAGAAAGAGTCGCAAGGCAGAAGAGTGCAGAGAAGGAAGAAGGTCTTAGGAGTTCCCCTCCTTTTCAAGCTCTCACGAAGAACTTTTGTGAAGCGATTAAACTTAGTCCCACAGTATCTTGCTCCTCGTGCCCAATCTCTTCTCCAAAACTCTATCCCAAATCTCTCCACCTCCTCCGCCGCCTCCTTTTCCTCCTCCTCGTGCTTATCAGATTCCACGACAAAAATACCACAAAATTCCCGCCAAATAACCACAACTACCGTTTACGTACCCACATGGCAGGCCCCCATTCGGCATCCACTTACACACTACAAGTCAAAAAACACAGAGATGCACAGCCCCGCGGGCCCCACCTTCCCACCACCACTAATCGATCACTCTCCTAATCCTCTCTGTACTCAAGCTGGCACCGCCGTCTCCTTCTCCACTTCTACTAGATCGCATTTTAACAAACACTTCAACATGCTGCGGCTGCCGCAATTCACCTGCTGTTTCCTCCTCTTACTGCTCCCGTTGATCACGTGCTCGAATTCGAAGCCGACGACGGTGTACGACGTGCTGCGGGCCCACGGGCTTCCGATGGGACTGCTGCCGAAAGGGGTCGTCGATTTCGAGGTCGACGATACCGGCAGGTTTACGGCCCACCTGGATCAGGCCTGCAATGCCAAGTTCGAGAGCGAGTTGCATTACGACAGAAACGTTTCGGGGATGCTGAGTTTCGGGCGGATCGGATCCTTGTCGGGTATTTCGGCGCAGGAGCTGTTCCTCTGGTTTCCGGTTAAGGGTATCCGGATCGACGTGCCCAACTCCGGCATCATTTACTTCGATGTCGGCGTCGTGTATAAGCAGTTCTCGCTGTCGCTGTTCGAAACGCCGCCTGATTGCACGGCCGCCTCCGGCAGCTTTGAAGCCATTGGAACCGTTTCCAAGAGTAAACCGGGGAAATTGCGGTCCAAGCTCAATTATGAACTCGGTGGGAGATATTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

353

Amino Acids

38.85

Weight (kDa)

9.53

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 560
Acc36I ACCTGC 2 cut(s) 560, 687
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 684
AccII CGCG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 855
AclI AACGTT 1 cut(s) 765
AclWI GGATC 5 cut(s) 723, 792, 800, 805, 866
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 2 cut(s) 301, 595
AcvI CACGTG 1 cut(s) 588
AcyI GRCGYC 1 cut(s) 900
AfaI GTAC 3 cut(s) 336, 463, 617
AfiI CCNNNNNNNGG 4 cut(s) 357, 718, 848, 875
AgsI TTSAA 3 cut(s) 133, 526, 974
AjiI CACGTC 2 cut(s) 622, 865
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 5 cut(s) 136, 470, 826, 969, 1024
AluI AGCT 5 cut(s) 136, 470, 826, 969, 1024
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 1 cut(s) 487
AlwI GGATC 5 cut(s) 723, 792, 800, 805, 866
AlwNI CAGNNNCTG 1 cut(s) 826
Aor13HI TCCGGA 1 cut(s) 855
AoxI GGCC 6 cut(s) 348, 412, 630, 705, 720, 954
ApaI GGGCCC 2 cut(s) 416, 634
ApeKI GCWGC 5 cut(s) 532, 538, 625, 655, 966
ApoI RAATTY 2 cut(s) 301, 595
Asp700I GAANNNNTTC 2 cut(s) 766, 988
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 7 cut(s) 349, 412, 413, 630, 631, 706, 1017
AsuC2I CCSGG 1 cut(s) 1005
AsuHPI GGTGA 1 cut(s) 541
AsuII TTCGAA 2 cut(s) 599, 933
AvaII GGWCC 1 cut(s) 1017
BaeGI GKGCMC 4 cut(s) 199, 416, 634, 870
BamHI GGATCC 1 cut(s) 797
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 2 cut(s) 416, 634
BauI CACGAG 2 cut(s) 192, 265
BbrPI CACGTG 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 5 cut(s) 519, 525, 612, 642, 978
BccI CCATC 2 cut(s) 4, 640
BceAI ACGGC 3 cut(s) 464, 720, 969
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 1 cut(s) 713
BciVI GTATCC 1 cut(s) 863
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 1 cut(s) 1005
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 1 cut(s) 501
BfuAI ACCTGC 2 cut(s) 560, 687
BfuI GTATCC 1 cut(s) 863
BglI GCCNNNNNGGC 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 713, 1005
Bme18I GGWCC 1 cut(s) 1017
BmgBI CACGTC 2 cut(s) 622, 865
BmgT120I GGNCC 7 cut(s) 349, 412, 413, 630, 631, 706, 1017
BmiI GGNNCC 7 cut(s) 351, 414, 415, 475, 632, 799, 985
BmrFI CCNGG 2 cut(s) 713, 1005
BmsI GCATC 4 cut(s) 370, 387, 765, 888
BplI GAGNNNNNCTC 2 cut(s) 108, 140
Bpu14I TTCGAA 2 cut(s) 599, 933
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 1 cut(s) 1005
Bsa29I ATCGAT 1 cut(s) 438
BsaAI YACGTR 2 cut(s) 334, 588
BsaHI GRCGYC 1 cut(s) 900
BsaJI CCNNGG 3 cut(s) 408, 634, 1004
BsaWI WCCGGW 2 cut(s) 841, 855
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 718, 848, 875
Bse118I RCCGGY 1 cut(s) 692
Bse3DI GCAATG 1 cut(s) 733
BseAI TCCGGA 1 cut(s) 855
BseBI CCWGG 1 cut(s) 713
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 408, 634, 1004
BseGI GGATG 2 cut(s) 361, 780
BseLI CCNNNNNNNGG 4 cut(s) 357, 718, 848, 875
BseMI GCAATG 1 cut(s) 733
BseMII CTCAG 1 cut(s) 770
BseRI GAGGAG 5 cut(s) 180, 229, 250, 253, 554
BseSI GKGCMC 4 cut(s) 199, 416, 634, 870
BseX3I CGGCCG 1 cut(s) 954
BseXI GCAGC 5 cut(s) 519, 525, 612, 642, 978
BsgI GTGCAG 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 6 cut(s) 350, 414, 632, 707, 722, 956
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 438
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 6 cut(s) 693, 842, 856, 876, 963, 1004
BslFI GGGAC 2 cut(s) 158, 663
BslI CCNNNNNNNGG 4 cut(s) 357, 718, 848, 875
BsmAI GTCTC 1 cut(s) 487
BsmBI CGTCTC 1 cut(s) 487
BsmFI GGGAC 2 cut(s) 158, 663
BsnI GGCC 6 cut(s) 350, 414, 632, 707, 722, 956
Bsp119I TTCGAA 2 cut(s) 599, 933
Bsp120I GGGCCC 2 cut(s) 412, 630
Bsp1286I GDGCHC 5 cut(s) 199, 416, 593, 634, 870
Bsp13I TCCGGA 1 cut(s) 855
Bsp143I GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
BspANI GGCC 6 cut(s) 350, 414, 632, 707, 722, 956
BspCNI CTCAG 1 cut(s) 771
BspDI ATCGAT 1 cut(s) 438
BspEI TCCGGA 1 cut(s) 855
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 7 cut(s) 351, 414, 415, 475, 632, 799, 985
BspMI ACCTGC 2 cut(s) 560, 687
BspPI GGATC 5 cut(s) 723, 792, 800, 805, 866
BspT104I TTCGAA 2 cut(s) 599, 933
BspT107I GGYRCC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 733
BsrFI RCCGGY 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 3 cut(s) 408, 634, 1004
BssMI GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
BssNI GRCGYC 1 cut(s) 900
BssSI CACGAG 2 cut(s) 192, 265
Bst2BI CACGAG 2 cut(s) 192, 265
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 5 cut(s) 33, 180, 328, 613, 988
Bst6I CTCTTC 3 cut(s) 60, 82, 210
BstACI GRCGYC 1 cut(s) 900
BstBAI YACGTR 2 cut(s) 334, 588
BstBI TTCGAA 2 cut(s) 599, 933
BstC8I GCNNGC 5 cut(s) 348, 412, 472, 630, 724
BstDEI CTNAG 3 cut(s) 112, 169, 779
BstDSI CCRYGG 2 cut(s) 408, 634
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 2 cut(s) 361, 780
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 8 cut(s) 19, 442, 506, 585, 718, 795, 800, 861
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 8 cut(s) 194, 411, 476, 538, 541, 728, 919, 1021
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 1 cut(s) 532
BstSCI CCNGG 2 cut(s) 711, 1003
BstSLI GKGCMC 4 cut(s) 199, 416, 634, 870
BstSNI TACGTA 1 cut(s) 334
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 5 cut(s) 519, 525, 612, 642, 978
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BstZI CGGCCG 1 cut(s) 954
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 1 cut(s) 863
BsuRI GGCC 6 cut(s) 350, 414, 632, 707, 722, 956
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 2 cut(s) 408, 634
BtrI CACGTC 2 cut(s) 622, 865
BtsCI GGATG 2 cut(s) 361, 780
BveI ACCTGC 2 cut(s) 560, 687
Cac8I GCNNGC 5 cut(s) 348, 412, 472, 630, 724
CaiI CAGNNNCTG 1 cut(s) 826
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 7 cut(s) 349, 412, 413, 630, 631, 706, 1017
Cfr42I CCGCGG 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 889
Csp6I GTAC 3 cut(s) 335, 462, 616
CviAII CATG 3 cut(s) 7, 342, 529
CviQI GTAC 3 cut(s) 335, 462, 616
DdeI CTNAG 3 cut(s) 112, 169, 779
DpnI GATC 8 cut(s) 18, 441, 505, 584, 717, 794, 799, 860
DpnII GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
EaeI YGGCCR 1 cut(s) 954
EagI CGGCCG 1 cut(s) 954
Eam1104I CTCTTC 3 cut(s) 60, 82, 210
EarI CTCTTC 3 cut(s) 60, 82, 210
EciI GGCGGA 2 cut(s) 232, 805
EclXI CGGCCG 1 cut(s) 954
Eco105I TACGTA 1 cut(s) 334
Eco147I AGGCCT 1 cut(s) 722
Eco24I GRGCYC 2 cut(s) 416, 634
Eco47I GGWCC 1 cut(s) 1017
Eco52I CGGCCG 1 cut(s) 954
Eco72I CACGTG 1 cut(s) 588
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoO109I RGGNCCY 2 cut(s) 349, 413
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 1 cut(s) 711
EcoT38I GRGCYC 2 cut(s) 416, 634
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 3 cut(s) 10, 345, 532
FaiI YATR 7 cut(s) 8, 22, 52, 343, 530, 909, 1032
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 158, 663
FatI CATG 3 cut(s) 6, 341, 528
FauI CCCGC 4 cut(s) 314, 403, 415, 621
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 1 cut(s) 684
FokI GGATG 2 cut(s) 348, 787
FriOI GRGCYC 2 cut(s) 416, 634
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 819
HaeIII GGCC 6 cut(s) 350, 414, 632, 707, 722, 956
HapII CCGG 6 cut(s) 693, 842, 856, 876, 963, 1004
HgaI GACGC 1 cut(s) 889
HhaI GCGC 1 cut(s) 820
Hin1I GRCGYC 1 cut(s) 900
Hin1II CATG 3 cut(s) 10, 345, 532
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 2 cut(s) 74, 278
HpaII CCGG 6 cut(s) 693, 842, 856, 876, 963, 1004
HphI GGTGA 1 cut(s) 541
Hpy166II GTNNAC 5 cut(s) 331, 616, 685, 702, 1001
Hpy188I TCNGA 4 cut(s) 64, 277, 645, 797
Hpy188III TCNNGA 3 cut(s) 141, 739, 856
Hpy8I GTNNAC 5 cut(s) 331, 616, 685, 702, 1001
Hpy99I CGWCG 7 cut(s) 610, 613, 623, 674, 689, 866, 905
HpyAV CCTTC 4 cut(s) 93, 100, 430, 496
HpyCH4III ACNGT 5 cut(s) 33, 180, 328, 613, 988
HpyCH4IV ACGT 5 cut(s) 333, 587, 621, 765, 864
HpyCH4V TGCA 5 cut(s) 94, 400, 726, 751, 951
HpyF10VI GCNNNNNNNGC 8 cut(s) 194, 411, 476, 538, 541, 728, 919, 1021
HpyF3I CTNAG 3 cut(s) 112, 169, 779
HpySE526I ACGT 5 cut(s) 333, 587, 621, 765, 864
Hsp92I GRCGYC 1 cut(s) 900
Hsp92II CATG 3 cut(s) 10, 345, 532
HspAI GCGC 1 cut(s) 818
Kpn2I TCCGGA 1 cut(s) 855
Ksp22I TGATCA 1 cut(s) 582
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
LmnI GCTCC 3 cut(s) 193, 579, 823
Lsp1109I GCAGC 5 cut(s) 519, 525, 612, 642, 978
LweI GCATC 4 cut(s) 370, 387, 765, 888
MaeI CTAG 1 cut(s) 501
MaeII ACGT 5 cut(s) 333, 587, 621, 765, 864
MalI GATC 8 cut(s) 18, 441, 505, 584, 717, 794, 799, 860
MboI GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
MboII GAAGA 5 cut(s) 77, 99, 115, 156, 197
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 5 cut(s) 199, 416, 593, 634, 870
MluCI AATT 5 cut(s) 301, 545, 595, 1010, 1027
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 42
MroI TCCGGA 1 cut(s) 855
MroXI GAANNNNTTC 2 cut(s) 766, 988
MseI TTAA 3 cut(s) 164, 513, 846
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 6 cut(s) 693, 842, 856, 876, 963, 1004
MspR9I CCNGG 2 cut(s) 713, 1005
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 8 cut(s) 194, 411, 476, 538, 541, 728, 919, 1021
NciI CCSGG 1 cut(s) 1005
NdeII GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
NlaIII CATG 3 cut(s) 10, 345, 532
NlaIV GGNNCC 7 cut(s) 351, 414, 415, 475, 632, 799, 985
NspI RCATGY 1 cut(s) 532
NspV TTCGAA 2 cut(s) 599, 933
PaqCI CACCTGC 1 cut(s) 560
PceI AGGCCT 1 cut(s) 722
PcsI WCGNNNNNNNCGW 5 cut(s) 605, 675, 684, 762, 897
PdmI GAANNNNTTC 2 cut(s) 766, 988
PfeI GAWTC 1 cut(s) 278
PleI GAGTC 1 cut(s) 82
PmaCI CACGTG 1 cut(s) 588
PmlI CACGTG 1 cut(s) 588
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 2 cut(s) 334, 588
Psp1406I AACGTT 1 cut(s) 765
Psp6I CCWGG 1 cut(s) 711
PspCI CACGTG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 7 cut(s) 351, 414, 415, 475, 632, 799, 985
PspOMI GGGCCC 2 cut(s) 412, 630
PspPI GGNCC 7 cut(s) 349, 412, 413, 630, 631, 706, 1017
PsrI GAACNNNNNNTAC 2 cut(s) 899, 931
PstNI CAGNNNCTG 1 cut(s) 826
PsuI RGATCY 1 cut(s) 797
RsaI GTAC 3 cut(s) 336, 463, 617
RsaNI GTAC 3 cut(s) 335, 462, 616
RseI CAYNNNNRTG 1 cut(s) 395
SacII CCGCGG 1 cut(s) 411
SalI GTCGAC 1 cut(s) 683
SaqAI TTAA 3 cut(s) 164, 513, 846
Sau3AI GATC 8 cut(s) 16, 439, 503, 582, 715, 792, 797, 858
Sau96I GGNCC 7 cut(s) 349, 412, 413, 630, 631, 706, 1017
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 713, 1005
SduI GDGCHC 5 cut(s) 199, 416, 593, 634, 870
SfaNI GCATC 4 cut(s) 370, 387, 765, 888
Sfr303I CCGCGG 1 cut(s) 411
SfuI TTCGAA 2 cut(s) 599, 933
SgrBI CCGCGG 1 cut(s) 411
SinI GGWCC 1 cut(s) 1017
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 465
SmoI CTYRAG 1 cut(s) 465
SnaBI TACGTA 1 cut(s) 334
Sse9I AATT 5 cut(s) 301, 545, 595, 1010, 1027
SseBI AGGCCT 1 cut(s) 722
SspMI CTAG 1 cut(s) 501
StuI AGGCCT 1 cut(s) 722
StyD4I CCNGG 2 cut(s) 711, 1003
TaaI ACNGT 5 cut(s) 33, 180, 328, 613, 988
TaiI ACGT 5 cut(s) 336, 590, 624, 768, 867
TasI AATT 5 cut(s) 301, 545, 595, 1010, 1027
TatI WGTACW 1 cut(s) 461
TauI GCSGC 5 cut(s) 248, 538, 544, 943, 959
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 3 cut(s) 164, 513, 846
Tru9I TTAA 3 cut(s) 164, 513, 846
TseI GCWGC 5 cut(s) 532, 538, 625, 655, 966
TspDTI ATGAA 1 cut(s) 1047
VpaK11BI GGWCC 1 cut(s) 1017
XagI CCTNNNNNAGG 1 cut(s) 716
XapI RAATTY 2 cut(s) 301, 595
XceI RCATGY 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 684
XmnI GAANNNNTTC 2 cut(s) 766, 988
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.